Extracolonic manifestations of Gardner syndrome: A case report

Blackwell McKenzie C,Thakkar Bhushan,Flores Andres,Zhang Wenjian 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.2

Gardner syndrome has head and neck manifestations that may be recognized during dental visits. Features such as multiple gnathic osteomas, impacted supernumerary teeth, and multiple foci of idiopathic osteosclerosis can be easily identified on dental radiographs, prompting the clinician to refer the patient for further investigation. A dental examination and routine radiographs play a vital role in revealing the extracolonic presentation of Gardner syndrome, which facilitates timely screening and detection of colorectal cancer and other malignancies associated with this condition. This report discusses the case of a 50-year-old Caucasian man who presented with a hard swelling of the left angle of the mandible and was diagnosed with Gardner syndrome based on abnormal findings from an oral examination, dental imaging, and medical and family history.

The Ecology of Vibrio vulnificus, Vibrio cholerae, and Vibrio parahaemolyticus in North Carolina Estuaries

Karen Dyer Blackwell,James D. Oliver 한국미생물학회 2008 The journal of microbiology Vol.46 No.2

While numerous studies have characterized the distribution and/or ecology of various pathogenic Vibrio spp., here we have simultaneously examined several estuarine sites for Vibrio vulnificus, V. cholerae, and V. parahaemolyticus. For a one year period, waters and sediment were monitored for the presence of these three pathogens at six different sites on the east coast of North Carolina in the United States. All three pathogens, identified using colony hybridization and PCR methods, occurred in these estuarine environments, although V. cholerae occurred only infrequently and at very low levels. Seventeen chemical, physical, and biological parameters were investigated, including salinity, water temperature, turbidity, dissolved oxygen, levels of various inorganic nutrients and dissolved organic carbon, as well as total vibrios, total coliforms, and E. coli. We found each of the Vibrio spp. in water and sediment to correlate to several of these environmental measurements, with water temperature and total Vibrio levels correlating highly (P<0.0001) with occurrence of the three pathogens. Thus, these two parameters may represent simple assays for characterizing the potential public health hazard of estuarine waters.

X-ray Analysis of the Structure of the Thermotropic Liquid Crystalline Copolyesters

홍성권,John Blackwell 한국고분자학회 1990 한국고분자학회 학술대회 연구논문 초록집 Vol.1990 No.10

Purification of the Clonorchis Antigen using a Diethylaminoethyl-Sephadex A50 Column

Kim, Je Hyun,Blackwell, R. Q. Yonsei University, College of Medicine 1963 Yonsei medical journal Vol.4 No.-

Biochars Influence Nitrogen Leaching and Availability to Wheat Plants

Zakaria Solaiman,Paul Blackwell,Lynette Abbott,Daniel Murphy 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

A Comparison of the Cationic Ring-Opening Polymerizations of 3-Oxetanol and Glycidol

A. Timothy Royappa,Mitra R. Vashi,Cholena L. Russo,Aaron C. Blackwell 한국고분자학회 2013 Macromolecular Research Vol.21 No.10

The technologically important polymers poly(3-oxetanol) and polyglycidol (sometimes referred to as polyglycerol) were both synthesized in high yield by a one-pot BF3-catalyzed cationic ring-opening polymerization of the respective monomers in dichloromethane at ambient temperature. The polymerization reactions and the resulting polymers were compared. The polymerization of 3-oxetanol was less exothermic than the polymerization of glycidol under identical reaction conditions, because of the lower ring strain in 3-oxetanol than in glycidol, confirmed by semi-empirical calculations. The resulting polymers were similar, i.e., they had similar Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectra, molecular weights, thermal properties and physical characteristics. However, the poly(3-oxetanol) had a higher ratio of secondary to primary alcohols than did polyglycidol.

Poster Session : Immunology ; Analysis of tuberculosis susceptibility genes in the chemokine-genes cluster region of chromosome 17 in Chinese

( Nelson LS Tang ),( CC Leung ),( CM Tam ),( KM Kam ),( Kathy Kong ),( J Blackwell ),( CY Chan ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Overview of the HUPO Plasma Proteome Project: Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database

Omenn, Gilbert ,S.,States, David ,J.,Adamski, Marcin,Blackwell, Thomas ,W.,Menon, Rajasree,Hermjakob, Henning,Apweiler, Rolf,Haab, Brian ,B.,Simpson, Richard ,J.,Eddes, J WILEY-VCH 2005 PROTEOMICS -WEINHEIM- Vol.5 No.13

<P>HUPO initiated the Plasma Proteome Project (PPP) in 2002. Its pilot phase has (1) evaluated advantages and limitations of many depletion, fractionation, and MS technology platforms; (2) compared PPP reference specimens of human serum and EDTA, heparin, and citrate-anti-coagulated plasma; and (3) created a publicly-available knowledge base (www.bioinformatics.med.umich.edu/hupo/ppp; www.ebi.ac.uk/pride). Thirty-five participating laboratories in 13 countries submitted datasets. Working groups addressed (a) specimen stability and protein concentrations; (b) protein identifications from 18 MS/MS datasets; (c) independent analyses from raw MS-MS spectra; (d) search engine performance, subproteome analyses, and biological insights; (e) antibody arrays; and (f) direct MS/SELDI analyses. MS-MS datasets had 15 710 different International Protein Index (IPI) protein IDs; our integration algorithm applied to multiple matches of peptide sequences yielded 9504 IPI proteins identified with one or more peptides and 3020 proteins identified with two or more peptides (the Core Dataset). These proteins have been characterized with Gene Ontology, InterPro, Novartis Atlas, OMIM, and immunoassay-based concentration determinations. The database permits examination of many other subsets, such as 1274 proteins identified with three or more peptides. Reverse protein to DNA matching identified proteins for 118 previously unidentified ORFs.</P><P>We recommend use of plasma instead of serum, with EDTA (or citrate) for anticoagulation. To improve resolution, sensitivity and reproducibility of peptide identifications and protein matches, we recommend combinations of depletion, fractionation, and MS/MS technologies, with explicit criteria for evaluation of spectra, use of search algorithms, and integration of homologous protein matches.</P><P>This Special Issue of PROTEOMICS presents papers integral to the collaborative analysis plus many reports of supplementary work on various aspects of the PPP workplan. These PPP results on complexity, dynamic range, incomplete sampling, false-positive matches, and integration of diverse datasets for plasma and serum proteins lay a foundation for development and validation of circulating protein biomarkers in health and disease.</P>

Interhomolog polymorphism shapes meiotic crossover within the Arabidopsis <i>RAC1</i> and <i>RPP13</i> disease resistance genes

Serra, Heï,di,Choi, Kyuha,Zhao, Xiaohui,Blackwell, Alexander R.,Kim, Juhyun,Henderson, Ian R. Public Library of Science 2018 PLoS genetics Vol.14 No.12

<▼1><P>During meiosis, chromosomes undergo DNA double-strand breaks (DSBs), which can be repaired using a homologous chromosome to produce crossovers. Meiotic recombination frequency is variable along chromosomes and tends to concentrate in narrow hotspots. We mapped crossover hotspots located in the <I>Arabidopsis thaliana RAC1</I> and <I>RPP13</I> disease resistance genes, using varying haplotypic combinations. We observed a negative non-linear relationship between interhomolog divergence and crossover frequency within the hotspots, consistent with polymorphism locally suppressing crossover repair of DSBs. The <I>fancm</I>, <I>recq4a recq4b</I>, <I>figl1</I> and <I>msh2</I> mutants, or lines with increased <I>HEI10</I> dosage, are known to show increased crossovers throughout the genome. Surprisingly, <I>RAC1</I> crossovers were either unchanged or decreased in these genetic backgrounds, showing that chromosome location and local chromatin environment are important for regulation of crossover activity. We employed deep sequencing of crossovers to examine recombination topology within <I>RAC1</I>, in wild type, <I>fancm</I>, <I>recq4a recq4b</I> and <I>fancm recq4a recq4b</I> backgrounds. The <I>RAC1</I> recombination landscape was broadly conserved in the anti-crossover mutants and showed a negative relationship with interhomolog divergence. However, crossovers at the <I>RAC1</I> 5′-end were relatively suppressed in <I>recq4a recq4b</I> backgrounds, further indicating that local context may influence recombination outcomes. Our results demonstrate the importance of interhomolog divergence in shaping recombination within plant disease resistance genes and crossover hotspots.</P></▼1><▼2><P><B>Author summary</B></P><P>Sexually reproducing plants and animals produce gametes with half the number of chromosomes, which can participate in fertilization. A specialized cell division called meiosis generates gametes, where the chromosomes are copied once and segregated twice. A further key feature of meiosis is that chromosomes physically pair and undergo reciprocal exchanges, called crossovers. Due to independent chromosome segregation and crossovers, meiosis creates gametes that are genetically diverse, which has a major effect on patterns of sequence variation in populations. Interestingly, the frequency of crossover is also highly variable along the lengths of chromosomes and tends to be concentrated in narrow hotspots. Here we studied two crossover hotspots in detail that are located within disease resistance genes, using the model plant Arabidopsis. We show that within these hotspots, greater levels of genetic difference between the recombining chromosomes locally inhibits crossover formation. We also show that hotspots within one of these resistance genes are surprisingly resistant to genetic backgrounds that increase crossovers elsewhere in the genome. This indicates that patterns of polymorphism and hotspot location along the chromosome are both important for control of recombination activity.</P></▼2>

Suppressed ubiquitination of Nrf2 by p47^phox contributes to Nrf2 activation

Ha Kim, Kyun,Sadikot, Ruxana T.,Yeon Lee, Ji,Jeong, Han-Sol,Oh, Yu-Kyoung,Blackwell, Timothy S.,Joo, Myungsoo Elsevier 2017 FREE RADICAL BIOLOGY AND MEDICINE Vol.113 No.-

<P><B>Abstract</B></P> <P>Although critical in phagocytosis in innate immunity, reactive oxygen species (ROS) collaterally inflict damage to host phagocytes because they indiscriminate targets. Since Nrf2 increases the expression of anti-oxidant enzymes that nullifies ROS, ROS activating Nrf2 is a critical negative regulatory step for countering the deleterious effects of ROS. Here, we postulate whether, along with ROS activating Nrf2, NADPH oxidase components also participate in direct activation of Nrf2, contributing to protection from ROS. Our results show that the p47<SUP>phox</SUP> of the NADPH oxidase, but not p65<SUP>phox</SUP> or p40<SUP>phox</SUP>, physically binds to Nrf2, activating the Nrf2 function. p47<SUP>phox</SUP> binding to Nrf2/Keap1 complex suppresses the ubiquitination of Nrf2, while p47<SUP>phox</SUP> becomes ubiquitinated by Keap1. p47<SUP>phox</SUP> increases the nuclear translocation of Nrf2 and the expression of Nrf2-dependent genes, whereas genetic ablation of p47<SUP>phox</SUP> decreases the expression of those genes. In a lipopolysaccharide-induced acute lung inflammation mouse model, selective expression of p47<SUP>phox</SUP> in mouse lungs induces the expression of Nrf2-dependent genes and is sufficient to suppress neutrophilic lung inflammation. Therefore, our findings suggest that p47<SUP>phox</SUP> is a novel regulator of Nrf2 function.</P> <P><B>Highlights</B></P> <P> <UL> <LI> p47<SUP>phox</SUP> of NADPH oxidase binds to Nrf2, without disrupting Nrf2/Keap1 complex. </LI> <LI> p47<SUP>phox</SUP> binding to Nrf2 results in the ubiquitination of p47<SUP>phox</SUP> by Keap1. </LI> <LI> p47<SUP>phox</SUP> suppresses the ubiquitination of Nrf2. </LI> <LI> p47<SUP>phox</SUP> induces the expression of Nrf2-dependent genes in cells and mouse lungs. </LI> <LI> p47<SUP>phox</SUP> suppresses inflammation in a LPS-induced lung inflammation mouse model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>