The differentiation of rat-induced pluripotent stem cells into alveolar type II epithelial cells with a three-step induction protocol

Bei Wu,Chen Wang,Feilong Hei,Cun Long,Mengmeng Chen,Shengnan Yang,Jie Yu,Zhihai Ju 한국통합생물학회 2015 Animal cells and systems Vol.19 No.1

Induced pluripotent stem (iPS) cells derive from autologous somatic cells, the application prospect of iPS cells forregenerative medicine and tissue engineering is better than embryonic stem cells (ESCs) to some extent. Alveolar type II(AT II) epithelial cells play key role in the injured lung tissue regeneration and function recovery. The differentiation of iPScells into AT II cells could provide available source for injured lung treatment. In this study, rat iPS (riPS) cells wereresuscitated and proliferated for 14 days before differentiation. A modified three-step induction protocol similar to thereported ESCs inducing procedure was used in this study for the differentiation groups. Routine cell culture was done to theriPS cell control group (riPS-con). At stage 3, cells of day 7 (Diff. 7) and day 14 (Diff. 14) were collected for the real-timepolymerase chain reaction tests for gene expressions of Oct4, Nanog, SPA, SPB, SPC, SPD, and CC10. Immunofluorescencestaining of SPC and SSEA-1 was conducted. At the end of the differentiation, cell morphology becameoutstretched and epithelium-like. Cells of the Diff. 14 group positively expressed SPC and negatively expressed SSEA-1,which is contrary to the riPS-con group. In the Diff. 7 and the Diff. 14 groups, the expression of Oct4, Nanog, and SPBdecreased (P < 0.05), whereas the expression of SPA, SPC, SPD (P < 0.05), and CC10 (P > 0.05) increased. This studyindicated that riPS cells can successfully differentiate into AT II epithelial cells with the three-step induction protocol andmay be further applied to implanting in decellularized rat lung scaffolds and building a bio-artificial lung.

온라인 생방송 플랫폼 이용 동기와 생방송 특성이 제품만족도와 지속적 쇼핑의도에 미치는 영향

오소패(Wu, Xiao Bei),황진숙(Hwang, Jin Sook) 한국디자인문화학회 2018 한국디자인문화학회지 Vol.24 No.2

인터넷의 발전은 온라인 생방송 마케팅의 발전을 촉진시켜왔다. 인터넷 플랫폼과 운영기술의 지속적인 발전으로 온라인 생방송 마케팅은 대단한 상업기능과 잠재력을 갖고 있다. 지금 중국내 C2C 거래 방식은 전자상거래에서 중요한 위치를 차지하고 있다. 최근 몇 년간, 온라인 생방송 마케팅시대의 도래에 따라 온라인 생방송의 어떤 요소들이 소비자의 만족도와 구매의도에 대해 영향을 미치는지 연구할 필요가 있다. 본 연구는 온라인 생방송 플랫폼을 이용하는 중국소비자를 대상으로 온라인 생방송 플랫폼 이용 동기와 온라인 생방송의 특성이 소비자의 제품 만족도와 지속적 쇼핑의도에 미치는 영향에 대해 알아보고자 한다. 본 연구의 대상은 중국에 거주하는 성인 남녀로 최근 6개월 사이에 온라인 생방송을 이용해 제품을 쇼핑 및 구매한 경험이 있는 소비자를 대상으로 하였으며, 2017년 11월 25일부터 12월4일까지 온라인 리서치 업체를 통해 설문조사를 실시하였고, 총 400명의 설문응답을 분석에 활용하였다. 최종분석방법으로는 spss 21.0을 활용하여 기술통계 탐색적 요인분석, 신뢰도분석, 상관분석을 실시하였으며, AMOS 21.0을 이용하여 확인적 요인분석, 구조방정식 경로분석을 실시하였다. 연구 결과는 다음과 같다. 첫째, 생방송 플랫폼 이용동기가 제품 만족도에 미치는 영향을 본 결과, 정보공유, 오락성, 공감대가 유의한 영향을 미치는 것으로 나타났다. 둘째, 온라인 생방송 특성이 제품 만족도에 미치는 영향을 본 결과, 효용성, 편리성, 즐거움, 신뢰성이 유의한 영향을 미치는 것으로 나타났다. 셋째, 생방송 플랫폼 이용 동기 중 정보공유, 공감대, 의사소통은 지속적 쇼핑의도에 유의한 영향을 미치는 것으로 나타났다. 넷째, 온라인 생방송 특성 중 효용성, 즉시성, 신뢰성은 지속적 쇼핑의도에 유의한 영향을 미치는 것으로 나타났다. 다섯째, 제품 만족도는 지속적 쇼핑의도에 유의한 영향을 미치는 것으로 나타났다. 본 연구의 결과는 온라인 생방송의 적절한 전략수립에 도움을 줄 수 있다. The development of the Internet has promoted the progress of the marketing of online broadcasting. Owing to the continuous progress of Internet platforms and operation technologies, the online broadcasting marketing has immense commercial functions and potential. Currently, the C2C transaction method takes an important position in electronic commerce. In light of the advent of the online broadcasting marketing age in recent years, it is necessary to study which elements of online broadcasting influence the satisfaction and the purchase intention of consumers. This study aimed to identify the effects of social broadcasting platform use intention and online broadcasting on the product satisfaction and the continuous shopping intention of consumers by targeting the Chinese consumers who are using online broadcasting platforms. The targets of this study - the adults residing in China - were the consumers who had an experience of purchasing items by using online broadcasting. The questionnaire survey was conducted from 25 November 2017 to 4 December the same year through an online research company and the 400 sample responses were used in the analysis. As the final analysis methods, the descriptive statistics exploratory factor analysis, reliability analysis, and correlation analysis were conducted by using SPSS 21.0 and confirmatory factor analysis and structural equation path analysis were carried out by using AMOS 21.0. The following are the results of this study. First, the online platform use intention significantly influenced the information sharing, the amusement, and the sympathy of product satisfaction. Second, the online live broadcasting characteristics significantly influenced the utility, the convenience, the pleasure, and the reliability of product satisfaction. Third, the live broadcasting platform use intention significantly influenced the information sharing, the sympathy, and the communication of continuous shopping intention. Fourth, online live-broadcasting characteristics had significant influence on the utility, the instantaneity, and the reliability of continuous shopping intention. Fifth, product satisfaction had significant influence on continuous shopping intention. Through the aforementioned analysis results, this study tried to provide practical implications to establishing adequate target marketing strategies of online live-broadcasting, which is standing out in C2C electronic commerce transactions.

Centromere protein U enhances the progression of bladder cancer by promoting mitochondrial ribosomal protein s28 expression

Liu, Bei-Bei,Ma, Tao,Sun, Wei,Gao, Wu-Yue,Liu, Jian-Min,Li, Li-Qiang,Li, Wen-Yong,Wang, Sheng,Guo, Yuan-Yuan The Korean Society of Pharmacology 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.2

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

Co-amplification at Lower Denaturation-temperature PCR Combined with Unlabled-probe High-resolution Melting to Detect KRAS Codon 12 and 13 Mutations in Plasma-circulating DNA of Pancreatic Adenocarcinoma Cases

Wu, Jiong,Zhou, Yan,Zhang, Chun-Yan,Song, Bin-Bin,Wang, Bei-Li,Pan, Bai-Shen,Lou, Wen-Hui,Guo, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: The aim of our study was to establish COLD-PCR combined with an unlabeled-probe HRM approach for detecting KRAS codon 12 and 13 mutations in plasma-circulating DNA of pancreatic adenocarcinoma (PA) cases as a novel and effective diagnostic technique. Materials and Methods: We tested the sensitivity and specificity of this approach with dilutions of known mutated cell lines. We screened 36 plasma-circulating DNA samples, 24 from the disease control group and 25 of a healthy group, to be subsequently sequenced to confirm mutations. Simultaneously, we tested the specimens using conventional PCR followed by HRM and then used target-DNA cloning and sequencing for verification. The ROC and respective AUC were calculated for KRAS mutations and/or serum CA 19-9. Results: It was found that the sensitivity of Sanger reached 0.5% with COLD-PCR, whereas that obtained after conventional PCR did 20%; that of COLD-PCR based on unlabeled-probe HRM, 0.1%. KRAS mutations were identified in 26 of 36 PA cases (72.2%), while none were detected in the disease control and/or healthy group. KRAS mutations were identified both in 26 PA tissues and plasma samples. The AUC of COLD-PCR based unlabeled probe HRM turned out to be 0.861, which when combined with CA 19-9 increased to 0.934. Conclusions: It was concluded that COLD-PCR with unlabeled-probe HRM can be a sensitive and accurate screening technique to detect KRAS codon 12 and 13 mutations in plasma-circulating DNA for diagnosing and treating PA.

Enhanced adsorption capacity of dyes by surfactant-modified layered double hydroxides from aqueous solution

Bei Zhang,Zhihao Dong,Dejun Sun,Tao Wu,Yujiang Li 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.49 No.-

The utilization of organo-modified layered double hydroxides with anionic surfactants (organo-LDHs) asadsorbents were successfully carried out to remove various synthetic dyes from aqueous solution. Intercalation of anionic surfactants changed the surface properties of MgAl-LDH from hydrophilic tohydrophobic, and a charge inversion occurred with increasing the length of surfactant chains. Suchchanges in the surface properties of the organo-LDHs were important. Experimental data shown that theSNS-modified MgAl-LDH could be used as a broad-spectrum adsorbent to effectively remove anionic,non-ionic, and cationic dyes from aqueous solution.

Conventional Ultrasonography and Real Time Ultrasound Elastography in the Differential Diagnosis of Degenerating Cystic Thyroid Nodules Mimicking Malignancy and Papillary Thyroid Carcinomas

Wu, Hong-Xun,Zhang, Bing-Jie,Wang, Jun,Zhu, Bei-Lin,Zang, Ya-Ping,Cao, Yue-Long Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: To evaluate the diagnostic utility of conventional ultrasonography and real time ultrasound elastography in differentiating degenerating cystic thyroid nodules mimicking malignancy from papillary thyroid carcinoma. Methods: We retrospectively analyzed conventional ultrasonographic and elastographic characteristics of 19 degenerating cystic thyroid nodules mimicking malignancy in 19 patients, with 30 surgically confirmed PTCs as controls. Based on size, the nodules had been grouped into less than 10mm (group A) and greater than 10 mm (group B). We evaluated conventional parameters and elasticity pattern. Color-scaled elastograms were graded as to stiffness of nodules using an elasticity pattern from I (soft) to IV (stiff). Results: Degenerating cystic thyroid nodules were similar to PTCs in conventional ultrasonographic findings, but the former frequently showed oval to round in shape (group A, 69.2% vs 18.8%, P=0.017; group B, 66.7% vs 7.14%, P=0.017) and punctuate hyperechoic foci (group A, 61.5% vs 0, P<0.001; group B, 50% vs 0, P<0.001). On real time ultrasound elastography, 7 of 13 degenerating cystic thyroid nodules in group A were pattern I, 5 were pattern II, 1 was pattern III. One degenerating cystic thyroid nodule in group B was pattern II, 5 were pattern III. The area under the curve for elastography was 0.98 in group A (sensitivity 92.3%, specificity 100%, P = 0.002), and 0.88 in group B (sensitivity 16.7%, specificity 100%, P = 0.014). Conclusions: As a dependable imaging technique, elastography helps increase the performance in differential diagnosis of degenerating cystic thyroid nodule and malignancy.

Elevated PIVKA-II is Associated with Early Recurrence and Poor Prognosis in BCLC 0-A Hepatocellular Carcinomas

Wang, Bei-Li,Tan, Qi-Wen,Gao, Xing-Hui,Wu, Jiong,Guo, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: To investigate the prognostic value of serum PIVKA-II (prothrombin induced by the absence of vitamin K or antagonist-II) in BCLC (Barcelona Clinic Liver Cancer) 0-A hepatocellular carcinoma (HCC) patients after curative resection. Materials and Methods: Preoperative sera were collected from 140 patients with BCLC 0-A HCCs undergoing curative resection during 2011-2012 in Zhongshan Hospital. Follow-up ended on November 2013. ELISA was used to detect the serum concentrations of preoperative PIVKA-II. The prognostic value of PIVKA-II and other clinicopathological factors was analyzed by the Kaplan-Meier method and the multivariate Cox proportional hazards model. Results: During follow-up, 39 of 140 patients suffered recurrence and the 1-year recurrence rate was 27.9%. The high-PIVKA-II expression group had lower 1-year time to progression (TTP) compared with the low-expression group (54.8% vs 20.2%, p<0.001). Patients with high preoperative PIVKA-II expression showed a relatively higher risk of developing postoperative recurrence than those with low expression in the low-recurrence-risk subgroups, including ${\alpha}$-fetoprotein ${\leq}400ng/mL$ (45.4% vs 16.7%; p=0.006), tumor size ${\leq}5cm$ (54.2% vs 18.1%; p<0.001), single tumor (56.0% vs 19.1%; p<0.001), absence of satellite lesions (53.3% vs 19.8%; p=0.001), absence of vascular invasion (52.6% vs 14.9%; p=0.002), and Edmondson stage I/II (60.9% vs 20.3%; p<0.001). PIVKA-II was the strongest independent prognostic factor for TTP (hazard ratio, 2.877; 95% CI 1.524-5.429; p=0.001). Conclusions: Elevated PIVKA-II is associated with early recurrence of BCLC 0-A HCC after curative resection and can be considered a novel prognostic predictor.

A Rapid, Green and Controllable Strategy to Fabricate Electrodeposition of Reduced Graphene Oxide Film as Sensing Materials for Determination of Taxifolin

Yanju Wu,Mingxiu Lv,Bei Li,Junda Ge,Lin Gao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.3

The electrodeposition of a film of reduced graphene oxide (ERGO) with well-dispersed on a glassy carbon electrode (GCE) was achieved using cyclic voltammetry (CV) in a graphene oxide (GO) colloidal solution. Using square wave adsorptive stripping voltammetry (SWASV), the ERGO film was applied for the first time, in developing a high-sensitive electrochemical sensor for detection of taxifolin. Compared with bare GCE, the resulting electrodes (ERGO/GCE) exhibited excellent response toward the oxidation of taxifolin by significantly enhancing the oxidation peak currents and the decreased peak-to-peak separation. Under selected conditions, the reduction peak currents had linear relationship with taxifolin concentration in the range of 5.0 x 10-8 - 1.5 x 10-5 mol L-1, with detection limit of 4.0 x 10-9 mol L-1. Besides, the method was successfully applied to the quantitative determination of taxifolin in the Prince's-feather fruit with satisfactory results.

Effect of Metabolic Structures and EnergyRequirements on Curdlan Production byAlcaligenes faecalis

Xiao Bei Zhan,Zhi-Yong Zheng,Jin Woo Lee,Zhongping Shi,Lei Wang,Li Zhu,Jian-Rong Wu,Chi Chung Lin 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4

A comprehensive metabolic network was proposed for Alcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated for curdlan batch production. Various metabolic structures and metabolic pathway distributions related with the curdlan maximal yield was evaluated. The results showed that the energy efficiency rather than the substrate supply was the major constraint for the enhancement of curdlan production. The increase in specific rate of glucose uptake could enhance curdlan production yield due to the decrease of the ratio of metabolic maintenance to substrate consumption. However, some of the energy loss and nutrient limitation associated with the increase of metabolic maintenance would adversely affect the conversion efficiency of the substrate.

Experimental study on all-bolted joint in modularized prefabricated steel structure

Zhanjing Wu,Zhong Tao,Bei Liu,Heng Zuo 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.73 No.6

The research study is focuses on a form of all-bolted joint with the external ring stiffening plate in the prefabricated steel structure. The components are bolted at site after being fabricated in the factory. Six specimens were tested under cyclic loading, and the effects of column axial compression ratio, concrete-filled column, beam flange sub plate, beam web angle cleats, and spliced column on the failure mode, hysteretic behavior and ductility of the joints were analyzed. The results shown that the proposed all-bolted joint with external ring stiffening plate performed high bearing capability, stable inflexibility degradation, high ductility and plump hysteretic curve. The primary failure modes were bucking at beam end, cracking at the variable section of the external ring stiffening plate, and finally welds fracturing between external ring stiffening plate and column wall. The bearing capability of the joints reduced with the axial compression ratio increased. The use of concrete-filled steel tube column can increase the bearing capability of joints. The existence of the beam flange sub plate, and beam web angle cleat improves the energy dissipation, ductility, bearing capacity and original rigidity of the joint, but also increase the stress concentration at the variable section of the external reinforcing ring plate. The proposed joints with spliced column also performed desirable integrity, large bearing capacity, initial stiffness and energy dissipation capacity for engineering application by reasonable design.