Cloning and Characterization of Directly Amplified Antiviral Gene Interferon Alpha-2b (HulFN$\{alpha}$-2b) from Human Leukocytes Chromosomal DNA

Behravan, Javad,Ahmadpour, Hassan The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.7

Interferons are cytokines that confer resistance to viral infection and inhibit cellular proliferation. The interferon alpha gene from human blood samples was amplified, cloned and expressed in E. coli (BL21). Leukocyte chromosomal DNA was used as a source of template DNA. Using specific primers, the gene for HulFN$\{alpha}$-2b was amplified and inserted into the E. coli vector, pET21b, by ligation of the HindIII and BamHI linkers of the vector and insert. The insert was further analyzed by PCR, DNA restriction mapping and sequencing, and expressed in a suitable E. coli strain. The production of this important cellular protein in the laboratory has significant applications in production of the recombinant pharmaceutical proteins.

Static response of 2-D functionally graded circular plate with gradient thickness and elastic foundations to compound loads

Behravan Rad, A. Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.2

In this paper, the static behavior of bi-directional functionally graded (FG) non-uniform thickness circular plate resting on quadratically gradient elastic foundations (Winkler-Pasternak type) subjected to axisymmetric transverse and in-plane shear loads is carried out by using state-space and differential quadrature methods. The governing state equations are derived based on 3D theory of elasticity, and assuming the material properties of the plate except the Poisson's ratio varies continuously throughout the thickness and radius directions in accordance with the exponential and power law distributions. The stresses and displacements distribution are obtained by solving state equations. The effects of foundation stiffnesses, material heterogeneity indices, geometric parameters and loads ratio on the deformation and stress distributions of the FG circular plate are investigated in numerical examples. The results are reported for the first time and the new results can be used as a benchmark solution for future researches.

Cloning and Characterization of Directly Amplified Antiviral Gene Interferon Alpha-2b (HuIFNα-2b) from Human Leukocytes Chromosomal DNA

Javad Behravan,Hassan Ahmadpour 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.7

Interferons are cytokines that confer resistance to viral infection and inhibit cellular proliferation. The interferon alpha gene from human blood samples was amplified, cloned and expressed in E. coli (BL21). Leukocyte chromosomal DNA was used as a source of template DNA. Using specific primers, the gene for HuIFNa-2b was amplified and inserted into the E. coli vector, pET21b, by ligation of the HindIII and BamHI linkers of the vector and insert. The insert was further analyzed by PCR, DNA restriction mapping and sequencing, and expressed in a suitable E. coli strain. The production of this important cellular protein in the laboratory has significant applications in production of the recombinant pharmaceutical proteins.

Static response of 2-D functionally graded circular plate with gradient thickness and elastic foundations to compound loads

A. Behravan Rad 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.2

In this paper, the static behavior of bi-directional functionally graded (FG) non-uniform thickness circular plate resting on quadratically gradient elastic foundations (Winkler-Pasternak type) subjected to axisymmetric transverse and in-plane shear loads is carried out by using state-space and differential quadrature methods. The governing state equations are derived based on 3D theory of elasticity, and assuming the material properties of the plate except the Poisson’s ratio varies continuously throughout the thickness and radius directions in accordance with the exponential and power law distributions. The stresses and displacements distribution are obtained by solving state equations. The effects of foundation stiffnesses, material heterogeneity indices, geometric parameters and loads ratio on the deformation and stress distributions of the FG circular plate are investigated in numerical examples. The results are reported for the first time and the new results can be used as a benchmark solution for future researches.

Static analysis of non-uniform heterogeneous circular plate with porous material resting on a gradient hybrid foundation involving friction force

A. Behravan Rad,M.R. Farzan-Rad,K. Mohammadi Majd 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.64 No.5

This paper is concerned with the static analysis of variable thickness of two directional functionally graded porous materials (FGPM) circular plate resting on a gradient hybrid foundation (Horvath-Colasanti type) with friction force and subjected to compound mechanical loads (e.g., transverse, in-plane shear traction and concentrated force at the center of the plate).The governing state equations are derived in terms of displacements based on the 3D theory of elasticity, assuming the elastic coefficients of the plate material except the Poisson‟s ratio varying continuously throughout the thickness and radial directions according to an exponential function. These equations are solved semi-analytically by employing the state space method (SSM) and one-dimensional differential quadrature (DQ) rule to obtain the displacements and stress components of the FGPM plate. The effect of concentrated force at the center of the plate is approximated with the shear force, uniformly distributed over the inner boundary of a FGPM annular plate. In addition to verification study and convergence analysis, numerical results are displayed to show the effect of material heterogeneity indices, foundation stiffness coefficients, foundation gradient indices, loads ratio, thickness to radius ratio, compressibility, porosity and friction coefficient of the foundation on the static behavior of the plate. Finally, the responses of FG and FG porous material circular plates to compound mechanical loads are compared.

Protective Effects of Aqueous and Ethanolic Extracts of Portulaca oleracea L. Aerial Parts on H_2O_2- Induced DNA Damage in Lymphocytes by Comet Assay

Javad Behravan,Fatemeh Mosafa,Negar Soudmand,Elahe Taghiabadi,Bibi Marjan Razavi,Gholamreza Karimi 사단법인약침학회 2011 Journal of Acupuncture & Meridian Studies Vol.4 No.3

The comet assay is a standard method for measuring DNA damage. In this study, the protective effects of ethanolic and aqueous extracts of Portulaca oleracea L. (P. oleracea)on human lymphocyte DNA lesions were evaluated with the comet assay. Lymphocytes were isolated from blood samples taken from healthy volunteers. Human lymphocytes were incubated in H_2O_2 (50,100, and 200 mM), aqueous extract (0.05, 0.1,0.5, 1, and 2.5 mg/ml), and ethanolic extracts (0.05, 0.1, 0.5, 1, and 2.5 mg/ml) of P. oleraceae aerial parts alone with a combination of H_2O_2 (100 mM) with either 1 or 2.5 mg/ml of both extracts at 4℃ for 30 minutes. The extent of DNA migration was measured using the alkaline single cell gel electrophoresis approach assay, and DNA damage was expressed as percentage tail DNA. We found that the aqueous extract of P. oleracea significantly inhibited DNA damage, while there was no effect of the ethanolic extract. These data suggest that the aqueous extract of P. oleracea can prevent oxidative DNA damage to human lymphocytes, which is likely due to antioxidant constituents in the extract.

Antigenotoxic Effects of Satureja hortensis L. on Rat Lymphocytes Exposed to Oxidative Stress

Mosaffa Fatemeh,Behravan Javad,Karimi Gholamreza,Iranshahi Mehrdad The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.2

The protective properties of Satureja hortensis L. on the rat lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy rats. DNA breaks and resistance to $H_{2}O_{2}$-induced damage were measured with the comet assay. Rat lymphocytes were incubated in S. hortensis ethanolic extract (SHE) (0.05, 0.1, 0.5, 1.0, and 2.5 mg/mL), essential oil (SHEO)(0.05, 0.1, 0.5, 1.0, and 2.5 ${mu}L/mL$), $H_{2}O_{2}$ (50, 100, and 200 ${\mu}M$), a combination of $H_{2}O_{2}$ (200 mM) with either SHE (1.0, 2.5 mg/mL) or SHEO (1.0, 2.5 ${\mu}L/mL$) at $4^{\circ}C$ for 30 min, and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Treatment of rat lymphocytes with SHE or SHEO resulted in significant reduction of $H_{2}O_{2}$-induced DNA damage compared to controls. SHE exhibited a significant (P<0.01) inhibitory effect on oxidative DNA damage at 2.5 mg/mL. SHEO (1.0 and 2.5 ${\mu}L/mL$) also showed significant inhibitory effects (P<0.01) on $H_{2}O_{2}$ induced chromosomal damage. In conclusion both the ethanolic extract and the essential oil of the plant reversed the oxidative damage to rat lymphocytes induced by hydrogen peroxide.

Antigenotoxic Effects of Satureja hortensis L. on Rat Lymphocytes Exposed to Oxidative Stress

Fatemeh Mosaffa,Javad Behravan,Gholamreza Karimi,Mehrdad Iranshahi 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.2

The protective properties of Satureja hortensis L. on the rat lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy rats. DNA breaks and resistance to H2O2-induced damage were measured with the comet assay. Rat lymphocytes were incubated in S. hortensis ethanolic extract (SHE) (0.05, 0.1, 0.5, 1.0, and 2.5 mg/ mL), essential oil (SHEO)(0.05, 0.1, 0.5, 1.0, and 2.5 µL/mL), H2O2 (50, 100, and 200 µM), a combination of H2O2 (200 mM) with either SHE (1.0, 2.5 mg/mL) or SHEO (1.0, 2.5 µL/mL) at 4oC for 30 min, and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Treatment of rat lymphocytes with SHE or SHEO resulted in significant reduction of H2O2-induced DNA damage compared to controls. SHE exhibited a significant (P<0.01) inhibitory effect on oxidative DNA damage at 2.5 mg/mL. SHEO (1.0 and 2.5 µL/mL) also showed significant inhibitory effects (P <0.01) on H2O2 induced chromosomal damage. In conclusion both the ethanolic extract and the essential oil of the plant reversed the oxidative damage to rat lymphocytes induced by hydrogen peroxide.

Cyclooxygenase-2 inhibition by novel Bisaryl imidazolyl imidazole derivatives increases Bax/Bcl-2 ratio and upregulates Caspase-3 gene expression in Caco-2 colorectal cancer cell line

Reza Entezari Heravi,Farzin Hadizadeh,Mojtaba Sankian,Jalil Tavakol Afshari,Javad Behravan 한국유전학회 2012 Genes & Genomics Vol.34 No.2

Cyclooxygenase-2 (COX-2) inhibitors including celecoxib inhibit cell growth and induce apoptosis in cancer cells. In this study, the relation of Bax (an apoptosis promoter) to Bcl-2(an apoptosis inhibitor) ratio with the apoptosis co-ordination enzyme, caspase-3 was investigated in correlation with the treatment of 4,5-bisaryl imidazolyl imidazoles as novel selective COX-2 inhibitors in Caco-2 colorectal cancer cells . Recently, the organic reactions under microwave irradiation attracted attention of scientists due to their high reaction rate,mild reaction conditions and the formation of clean products. Therefore, a microwave-assisted method was used to synthesize our compounds. The effects of these COX-2 inhibitors on the proliferation of Caco-2 cells were evaluated by MTT assay. cDNA microarray and clustering analysis were used to evaluate effects of our synthetic compounds on gene expression pattern of 112 genes involved in apoptosis pathways. Bax, Bcl-2 and caspase-3 mRNA expression and their relationship were analyzed by quantitative real-time PCR. Results indicated that proliferation of Caco-2 cells after treatment with 4,5-bisaryl imidazolyl imidazoles on Caco-2 cells were time and dose dependent. We conclude that increase in Bax/Bcl-2ratio leads to an up-regulation in caspase-3 mRNA expression.

Phorbol Ester TPA Modulates Chemoresistance in the Drug Sensitive Breast Cancer Cell Line MCF-7 by Inducing Expression of Drug Efflux Transporter ABCG2

Kalalinia, Fatemeh,Elahian, Fatemeh,Hassani, Mitra,Kasaeeian, Jamal,Behravan, Javad Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Recent studies have indicated a link between levels of cyclooxygenase-2 (COX-2) and development of the multidrug resistance (MDR) phenotype. The ATP-binding cassette sub-family G member 2 (ABCG2) is a major MDR-related transporter protein that is frequently overexpressed in cancer patients. In this study, we aimed to evaluate any positive correlation between COX-2 and ABCG2 gene expression using the COX-2 inducer 12-O-tetradecanoylphorbol-13-acetate (TPA) in human breast cancer cell lines. ABCG2 mRNA and protein expression was studied using real-time RT-PCR and flow cytometry, respectively. A significant increase of COX-2 mRNA expression (up to 11-fold by 4 h) was induced by TPA in MDA-MB-231 cells, this induction effect being lower in MCF-7 cells. TPA caused a considerable increase up to 9-fold in ABCG2 mRNA expression in parental MCF-7 cells, while it caused a small enhancement in ABCG2 expression up to 67 % by 4 h followed by a time-dependent decrease in ABCG2 mRNA expression in MDA-MB-231 cells. TPA treatment resulted in a slight increase of ABCG2 protein expression in MCF-7 cells, while a time-dependent decrease in ABCG2 protein expression was occurred in MDA-MB-231 cells. In conclusion, based on the observed effects of TPA in MDA-Mb-231 cells, it is proposed that TPA up-regulates ABCG2 expression in the drug sensitive MCF-7 breast cancer cell line through COX-2 unrelated pathways.