Attenuation of Peripheral Regulatory T-Cell Suppression of Skin-Homing CD8+T Cells in Atopic Dermatitis

Bao-Xiang Zhang,Gang Ding,Jun-Cheng Lyu,Hai-Bo Liu,Dian-Cai Zhang,Dian-Cai Zhang,Xing-Jie Bi,Zhi-Wu Duan 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.1

Purpose: Cutaneous lymphocyte-associated antigen (CLA)-expressing CD8+T cells have been known to play an important role in the pathogenesis of atopic dermatitis(AD). However, the mechanisms underlying the loss of self-tolerance remainunclear. Regulatory T cells (Tregs) play a key role in the development of homeostasisin the immune system. We, therefore, hypothesized that a reduced ability of Tregs to inhibit autologous CD8+CLA+T cells might be underlying mechanism in AD. Materials and Methods: CD8+CLA+T cells and Tregs were obtained from the peripheral blood of AD patients and control volunteers. The frequenciesof CD8+CLA+T cells were evaluated. The proliferative responses of CD8+CLA+T cells were assessed by flow cytometry, and the levels of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in culture supernatants were detected by enzyme-linked immunosorbent assay. Results: Our results revealed higher frequency and increased expression of perforin and granzyme-B in peripheralCD8+CLA+T cells in AD, and lower inhibitory ability of Tregs on proliferation of CD8+CLA+T cells in AD. Meanwhile, the levels of TGF-β1 produced by Tregs were significantly lower in AD, and anti-TGF-β1 abolished such suppression. Conclusion: The attenuated inhibitory ability of Tregs on hyper-activated autologousCD8+CLA+T cells, mediated by TGF-β1, plays an important role in the pathogenesis of AD.

Effect of carbon black content on microcellular structure and physical properties of chlorinated polyethylene rubber foams

Zhang, Bao Sheng,Lv, Xiu Feng,Zhang, Zhen Xiu,Liu, Yang,Kim, Jin Kuk,Xin, Zhen Xiang Elsevier 2010 Materials & design Vol.31 No.6

<P><B>Abstract</B></P><P>Chlorinated polyethylene rubber (CPE) foams filled with various content of carbon black were prepared by compression molding. The effect of carbon black content on the cure characteristics, foam characteristics and physical properties of the CPE compounds were investigated. The results showed that adding ZnSt as activator could lower the decomposition temperature of azodicarbonamide. The results of a moving die Rheometer suggested that the cure characteristics were influenced by the content of carbon black, and the cure rate index increased with carbon black content. The morphology and physical properties results indicated that the content of carbon black played important roles in the cell morphology and physical properties of CPE rubber foams.</P>

Genome-wide identification and analysis of long noncoding RNAs in longissimus muscle tissue from Kazakh cattle and Xinjiang brown cattle

Yan, Xiang-Min,Zhang, Zhe,Liu, Jian-Bo,Li, Na,Yang, Guang-Wei,Luo, Dan,Zhang, Yang,Yuan, Bao,Jiang, Hao,Zhang, Jia-Bao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11

Objective: In recent years, long noncoding RNAs (lncRNAs) have been identified in many species, and some of them have been shown to play important roles in muscle development and myogenesis. However, the differences in lncRNAs between Kazakh cattle and Xinjiang brown cattle remain undefined; therefore, we aimed to confirm whether lncRNAs are differentially expressed in the longissimus dorsi between these two types of cattle and whether differentially expressed lncRNAs regulate muscle differentiation. Methods: We used RNA-seq technology to identify lncRNAs in longissimus muscles from these cattle. The expression of lncRNAs were analyzed using StringTie (1.3.1) in terms of the fragments per kilobase of transcript per million mapped reads values of the encoding genes. The differential expression of the transcripts in the two samples were analyzed using the DESeq R software package. The resulting false discovery rate was controlled by the Benjamini and Hochberg's approach. KOBAS software was utilized to measure the expression of different genes in Kyoto encyclopedia of genes and genomes pathways. We randomly selected eight lncRNA genes and validated them by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: We found that 182 lncRNA transcripts, including 102 upregulated and 80 downregulated transcripts, were differentially expressed between Kazakh cattle and Xinjiang brown cattle. The results of RT-qPCR were consistent with the sequencing results. Enrichment analysis and functional annotation of the target genes revealed that the differentially expressed lncRNAs were associated with the mitogen-activated protein kinase, Ras, and phosphatidylinositol 3-kinase (PI3k)/Akt signaling pathways. We also constructed a lncRNA/mRNA coexpression network for the PI3k/Akt signaling pathway. Conclusion: Our study provides insights into cattle muscle-associated lncRNAs and will contribute to a more thorough understanding of the molecular mechanism underlying muscle growth and development in cattle.

Focus Improvement of High-Squint SAR Based on Azimuth Dependence of Quadratic Range Cell Migration Correction

Zhang, Shuang-Xi,Xing, Meng-Dao,Xia, Xiang-Gen,Zhang, Lei,Guo, Rui,Bao, Zheng IEEE 2013 IEEE geoscience and remote sensing letters Vol.10 No.1

<P>In this letter, we discuss the problem that linear range cell walk correction in the azimuth time domain may cause space variation along the azimuth not only to the quadratic phase but also to the quadratic range cell migration (QRCM) under the conditions of high resolution and large scene along the azimuth. Moreover, an algorithm is proposed to deal with this problem. The proposed algorithm adopts the azimuth space variation filtering in the range frequency domain. In addition, the range-dependence component of QRCM is corrected by linear chirp scaling, and the unified QRCM can be corrected in the 2-D frequency domain. The proposed algorithm, without interpolation, can be easily implemented by integrating with motion compensation for image processing. Simulation and airborne strip-map real data show the accuracy and efficiency of the proposed algorithm.</P>

Design, Synthesis and Antifungal Activities of Novel Strobilurin Derivatives Containing Pyrimidine Moieties

Xiang Zhang,Yong-Xin Gao,Hui-Jun Liu,Bao-Yuan Guo,Hui-Li Wang 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.8

Strobilurins are one of the most important classes of agricultural fungicides. To discover new strobilurin derivatives with high activity against resistant pathogens, a series of novel β-methoxyacrylate analogues were designed and synthesized by integrating substituted pyrimidine with a strobilurin pharmacophore. The compounds were confirmed and characterized by infrared, 1H nuclear magnetic resonance, elemental analysis and mass spectroscopy. The bioassays indicated that most of the compounds (1a-1h) exhibited potent antifungal activities against Colletotrichum orbiculare, Botrytis cinerea Pers and Phytophthora capsici Leonian at the concentration of 50 μg/mL. Exhilaratingly, compound 1d (R=3-trifluoromethylphenyl) showed better antifungal activity against all the tested fungi than the commercial strobilurin fungicide azoxystrobin.

Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2

Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

THEORETICAL INVESTIGATION OF SOLAR ENERGY HIGH TEMPERATURE HEAT STORAGE TECHNOLOGY BASED ON METAL HYDRIDES

XIANG-YU MENG,ZE-WEI BAO,FU-SHENG YANG,ZAO-XIAO ZHANG 대한설비공학회 2011 International Journal Of Air-Conditioning and Refr Vol.19 No.2

A solar energy storage system based on metal hydrides was proposed in this paper. The numerical simulation of processes of energy storage and thermal release were carried out. The dynamic behavior of heat and mass transfer in the metal hydride energy system were reported. Some factors which influence the whole system performance were discussed. The paper also made an economic analysis of the system, the results proved that the large amounts of metal hydride materials and the configurations of metal hydrides energy storage system involve a critical situation from an economical point of view. Then further analysis, particularly regarding the performance optimization and new plant arrangement of the metal hydrides energy storage system, has to be developed in order to attain the economical feasibility of the proposal.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens

Xiang-Dang Du,Xin-Sheng Li,Gui-Qin Wang,Bao-An Cui,Su-Mei Zhang,Jian-Zhong Shen 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.3

Seventy Escherichia coli isolates recovered from diseased chickens diagnosed with colibacillosis in Henan Province, China, between 2004 and 2005 were characterized for antimicrobial susceptibility profiles via a broth doubling dilution method. Overall, the isolates displayed resistance to trimethoprim-sulfamethoxazole (100%), oxytetracycline (100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively. Molecular detection revealed that the incidence rates of the floR, cmlA, cat1, cat2 and cat3 were 29, 31, 16, 13, and 0%, respectively. Additionally, 10% of the isolates were positive for both floR and cmlA. As these antimicrobial agents may potentially induce cross-resistance between animal and human bacterial pathogens, their prudent use in veterinary medicine is highly recommended.

Effects of confinement on physiological and psychological responses and expression of interleukin 6 and brain derived neurotrophic factor mRNA in primiparous and multiparous weaning sows

Zhang, Mingyue,Li, Xiang,Li, Jianhong,Sun, Hanqing,Zhang, Xiaohui,Bao, Jun Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

Objective: The present study aimed to investigate whether the long-lasting, recurrent restricting of sows leads to the physiological and psychological reaction of discomfort. Methods: Sows (Large White) that had experienced restricting for about 0.5 or 3 years and agematched sows kept in a group housing system (loose sows) were compared. Pupillary light reflex parameters were measured at the weaning stage. Immediately after slaughter, blood samples were taken to measure serum cortisol levels, and the brain was dissected, gene expression in the hippo-campus, frontal cortex and hypothalamus was analyzed. Results: The serum cortisol levels were higher in the confined sows than in the loose sows. The full maturity, but not the young adolescent, confined sows had longer latency time in the onset of pupil constriction than their loose counterparts. Real-time polymerase chain reaction analyses revealed an increased expression of interleukin 6 mRNA in the hippocampus and decreased expression of brain derived neurotrophic factor mRNA in hippocampus and hypothalamus and to a lesser extent in the frontal cortex of the full maturity confined sows, compared with the full maturity loose sows. Conclusion: Taken together, these data indicated that recurrent restricting stress in full maturity sows leads to the physiological and psychological reaction of discomfort.

Transgenic NfFeSOD Sedum alfredii plants exhibited profound growth impairments and better relative tolerance to long-term abiotic stresses

Xiang Gao,Wen-Li Ai,Huan Gong,Li-Juan Cui,Bo-Xia Chen,Hong-Yi Luo,Zhong-Chun Zhang,Bao-Sheng Qiu 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.2

Transgenic research was preformed by transferring a cyanobacterial (Nostoc flagelliforme) iron superoxide dismutase gene (NfFeSOD) into heavy metal hyperaccumulator Sedum alfredii via Agrobacterium-mediated method. Beyond expectation, NfFeSOD-overexpressing S. alfredii plants exhibited profound impairments, including plant growth retardation, abnormal root architecture, and reduced leaf greenness, photosynthetic efficiency and metal accumulation efficiency. Although transgenic plants appeared physiologically sensitive to high temperature, a higher relative biomass growth was still observed under long-term high temperature and osmotic stresses. Further investigation found that reactive oxygen species (ROS) homeostasis of transgenic plants was significantly affected, being ~50 % reduction of H2O2 level relative to wild-type plants. Gene transcription including ROS responsive genes was overall attenuated in transgenic plants, being more significant at normal temperature than at high temperature. In addition, ascorbate peroxidase (APX) activity was increased nearly twofolds in transgenic plants as compared to wild-type control. It may be inferred that ectopic NfFeSOD overexpression gives rise to a substantial increase of APX activity and leads to a sharp reduction of H2O2 level, thus impairing basal ROS signaling and plant growth. Specific genetic background of S. alfredii may be responsible for this sharp reduction of H2O2 level induced by NfFeSOD overexpression. S. alfredii plant has acclimated to elevated levels of ROS induced by heavy metals in native habitats and should require high ROS levels for basal signaling. We thus suppose that a sustained disturbance of high basal ROS signaling in metal hyperaccumulators may instead incur very sensitive response and thus result in profound growth impairments.