http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ji-Hae Park,Do-Gyeong Lee,Seung-Woo Yeon,Hyuk-Sang Kwon,Jong-Hee Ko,Dong-Jin Shin,Han-Sol Park,Yong-Soon Kim,Myun-Ho Bang,NAM-IN BAEK 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4
The silkworm (Bombyx mori L.) droppings were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned in succession with EtOAc, n-BuOH, and H_2O. From the EtOAc fraction, five megastigmane sesquiterpenes were isolated through repeated silica gel and ODS column chromatography. According to the results of spectroscopic data, such as NMR, MS, and IR, the chemical structures of the isolated compounds were determined as (3S,5R,8R)-3,5-dihydroxymegastigma-6,7-dien-9-one (1), (S)-dehydrovomifoliol (2), (6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one (3), (3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one (4), (6R,9R)-9-hydroxy-4-megastigmen-3-one (5). Compounds 2 through 5 were isolated for the first time from silkworm droppings. GC/MS analysis indicated silkworm powder contained compound 3, and mulberry leaves contained compound 4. Compounds 1 and 5 increased the expression of heme oxygenase-1 and SIRT1 in HepG2 and HEK239 cells, respectively. Heme oxygenase-1 is considered to be an antioxidant enzyme that catabolizes heme to carbon monoxide, free iron and biliverdin, while SIRT1 is the mammalian homologue of the yeast silent information regulator (Sir)-2, which are involved in the suppression of inflammatory mediators or factors that may be used to improve atopy-related symptoms.
Baek, Du-San,Kim, Jeong-Ho,Kim, Ye-Jin,Kim, Yong-Sung American Chemical Society 2018 MOLECULAR PHARMACEUTICS Vol.15 No.2
<P>Neuropilin-1 (NRP1), which functions as a coreceptor for vascular endothelial growth factor (VEGF) and is implicated in vascular permeability and tumorigenesis, has been targeted by peptides that specifically bind to the VEGF-binding region on NRP1. Like natural VEGF ligands, all known peptides with NRP1-binding activity bind only through a carboxy (C)-terminal R/K-x-x-R/K sequence motif (x stands for any amino acids); this strict requirement is called the C-end rule (CendR). Here, we report immunoglobulin Fc-fused NRP1-specific peptides deviating from CendR. We screened a yeast surface-displayed Fc-fused non-CendR peptide library against NRP1 and isolated Fc-V12, wherein V12 peptide comprising 12 amino acids has a PPRV sequence at its C-terminal end. Although Fc-V12 lacked the CendR motif, it showed selective binding to the VEGF-binding region of NRP1 and triggered cellular internalization of NRP1, which resulted in enhanced extravasation into tumor tissues and tumor tissue penetration of the Fc-fused peptide along with the coinjected chemical drug in tumor-bearing mice. Through a saturation mutagenesis study, we identified that the Val residue at the C-terminus of Fc-V12 is crucial for NRP1 binding. We further improved NRP1 affinity of Fc-V12 (<I>K</I><SUB>D</SUB> = ∼761 nM) through directed evolution of the upstream sequence of PPRV to obtain Fc-V12–33 (<I>K</I><SUB>D</SUB> = ∼17.4 nM), which exhibited enhanced NRP1-mediated vascular permeability as compared with Fc-V12. Our results provide functional Fc-fused non-CendR peptides, which bind to the VEGF-binding region of NRP1 and enhance vascular permeability, expanding the sequence space of NRP1-targeting peptides.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2018/mpohbp.2018.15.issue-2/acs.molpharmaceut.7b00761/production/images/medium/mp-2017-007613_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/mp7b00761'>ACS Electronic Supporting Info</A></P>
Development of Tungsten Heavy Alloy with Hybrid Structure for Kinetic Energy Penetrator
Baek Woon-Hyung,Kim Eun-Pyo,Song Heung-Sub,Hong Moon-Hee,Lee Seong,Kim Young-Moo,Lee Sung-Ho,Noh Joon-Woong,Ryu Joo-Ha 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1
A new tungsten heavy alloy with hybrid structure was manufactured for the kinetic energy penetrator. The tungsten heavy alloy is composed of two parts: core region is molybdenum added heavy alloy to promote the self-sharpening; outer part encompassing the core is conventional heavy alloy to sustain severe load in a muzzle during firing. From ballistic test, it was found that the penetration performance of the hybrid structure tungsten heavy alloy is higher than that of conventional heavy alloy. This heavy alloy is thought to be very useful for the penetrator in the near future.
Development of Comprehensive Separation System for ASR Recycling of ELVs
( Ho-seok Jeon ),( Sang-ho Baek ),( Byung-hun Go ),( Seong-min Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-
The recycling of End-of-Life Vehicles (ELVs) is getting great concerns according to the increase of a tremendous amount of ELVs worldwide. This led to various research works on the recycling of the ELVs that have resulted in technologies being developed and transferred to the industrialization. Generally, the reusable parts can be taken away in advance on the dismantling process of ELVs and the remaining are shredded, then the iron and nonferrous metals are recovered from the debris. About 75% of the ELVs are recycled but the remaining 25% are yet to be reclaimed or incinerated. This remaining 25% are called as Automobile Shredder Residues (ASR). Many technologies for the recycling of the ASR, such as compaction, solidification, pyrolysis, and gasification, have been researched and industrialized. However, the methods of compaction and solidification carry environmental pollution problems. And the pyrolysis and gasification methods may have higher treatment costs besides incurring the air pollution problem. Which can be inferred that the physical separation techniques and processes have better advantages in recovering the metal and recycling of plastics and rubber from the ASR, especially for the nations that have limited material resources, e.g. Korea. In this study, for material separation of ASR, a comprehensive separation system combining unit separation technique has been developed. The recycling rate was calculated by mass balance analysis of the recovered products through this system, and the total recycling rate was about to 86%.
Baek, Yonghyeon,Kim, Jinwoo,Hyunseok Kim,Jung, Seung Jin,Ryu, Ho,Kim, Suyeon,Son, Jeong-Yu,Um, Kyusik,Han, Sang Hoon,Seo, Hyung Jin,Heo, Juyoung,Lee, Kooyeon,Baik, Mu-Hyun,Lee, Phil Ho Royal Society of Chemistry 2019 Chemical Science Vol.10 No.9
<▼1><P>A novel method for the synthesis of acylmethyl-substituted 2-arylpyridine derivatives using 3-aryl-2<I>H</I>-azirines was developed.</P></▼1><▼2><P>A novel method for the synthesis of acylmethyl-substituted 2-arylpyridine derivatives using 3-aryl-2<I>H</I>-azirines was developed by exploring a prototype reaction using DFT-calculations and carrying out targeted experiments guided by the calculated mechanism. 2<I>H</I>-Azirine was initially hypothesized to ring-open at the metal center to furnish familiar metal nitrene complexes that may undergo C–N coupling. Computational studies quickly revealed and prototype experimental work confirmed that neither the formation of the expected metal nitrene complexes nor the C–N coupling were viable. Instead, azirine ring-opening followed by C–C coupling was found to be much more favorable to give imines that readily underwent hydrolysis in aqueous conditions to form acylmethyl-substituted products. This new method was highly versatile and selective toward a wide range of substrates with high functional group tolerance. The utility of the new method is demonstrated by a convenient one-pot synthesis of biologically relevant heterocycles such as pyridoisoindole and pyridoisoqunolinone.</P></▼2>
Anti-inflammatory activity of AP-SF, a ginsenoside-enriched fraction, from Korean ginseng
Baek, Kwang-Soo,Hong, Yong Deog,Kim, Yong,Sung, Nak Yoon,Yang, Sungjae,Lee, Kyoung Min,Park, Joo Yong,Park, Jun Seong,Rho, Ho Sik,Shin, Song Seok,Cho, Jae Youl The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.2
Background: Korean ginseng is an ethnopharmacologically valuable herbal plant with various biological properties including anticancer, antiatherosclerosis, antidiabetic, and anti-inflammatory activities. Since there is currently no drug or therapeutic remedy derived from Korean ginseng, we developed a ginsenoside-enriched fraction (AP-SF) for prevention of various inflammatory symptoms. Methods: The anti-inflammatory efficacy of AP-SF was tested under in vitro inflammatory conditions including nitric oxide (NO) production and inflammatory gene expression. The molecular events of inflammatory responses were explored by immunoblot analysis. Results: AP-SF led to a significant suppression of NO production compared with a conventional Korean ginseng saponin fraction, induced by both lipopolysaccharide and zymosan A. Interestingly, AP-SF strongly downregulated the mRNA levels of genes for inducible NO synthase, tumor necrosis factor-${\alpha}$, and cyclooxygenase) without affecting cell viability. In agreement with these observations, AP-SF blocked the nuclear translocation of c-Jun at 2 h and also reduced phosphorylation of p38, c-Jun N-terminal kinase, and TAK-1, all of which are important for c-Jun translocation. Conclusion: Our results suggest that AP-SF inhibits activation of c-Jun-dependent inflammatory events. Thus, AP-SF may be useful as a novel anti-inflammatory remedy.
Antibody response to pneumococcal vaccination in children with chronic or recurrent rhinosinusitis
Baek, Ji Hyeon,Seo, Hyun Kyong,Jee, Hye Mi,Shin, Youn Ho,Han, Man Yong,Oh, Eun Sang,Lee, Hyun Ju,Kim, Kyung Hyo The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.7
Purpose: Although chronic and recurrent rhinosinusitis is prevalent in children, little is known about its causes. Here, we investigated the humoral immunity in children with chronic or recurrent rhinosinusitis. Methods: We examined 16 children attending the outpatient clinic at the CHA Bundang Medical Center including 11 boys and 5 girls, aged 3-11 years (mean age, 5.6 years), who had rhinosinusitis for >3 months or >3 times per year. The complete blood count with differential and total serum concentrations of Immunoglobulin (Ig) E, IgA, IgD, IgM, IgG, and IgG subclasses ($IgG_1$, $IgG_2$, $IgG_3$, and $IgG_4$) of all children were measured. All subjects received 23-polysaccharide pneumococcal vaccination (PPV), and the levels of antibodies to 5 serologic types (4, 6B, 14, 18C, and 23F) of pneumococcal capsular polysaccharide antigens were measured before and after vaccination. Post-PPV antibody titers ${\geq}0.35{\mu}g/mL$ or with a ${\geq}4$-fold increase were considered as positive responses. Results: The titers of IgG, IgA, IgD, and IgM were within normal range in all 16 children, whereas the total IgE concentration was higher than normal in 2 children. $IgG_1$ deficiency was observed in 1 patient and $IgG_3$ deficiency in 3. After PPV, 1 patient failed to respond to all 5 serologic types, 2 failed to respond to 4 serologic types, and 2 failed to respond to 3 serologic types. Conclusion: Clinicians should consider the evaluation of humoral immune functions in children with chronic or recurrent rhinosinusitis who do not respond to prolonged antibiotic treatment.