The Dietary Food Components Capric Acid and Caprylic Acid Inhibit Virulence Factors in Candida albicans Through Multitargeting

Ashwini Jadhav,Supriya Mortale,Shivkrupa Halbandge,Priyanka Jangid,Rajendra Patil,Wasudev Gade,Kiran Kharat,Sankunny Mohan Karuppayil 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.11

Capric acid and caprylic acid are the dietary food components. They are found to inhibit the virulence factors like morphogenesis, adhesion, and biofilm formation in the human pathogenic yeast Candida albicans. Our study demonstrated that yeast-to-hyphal signal transduction pathways were affected by capric acid and caprylic acid. The expression profile of genes associated with serum-induced morphogenesis showed reduced expressions of Cdc35, Hwp1, Hst7, and Cph1 by the treatment with both the fatty acids. Cell elongation gene, Ece1, was surprisingly downregulated by 5208-fold by the treatment of caprylic acid. Nrg1 and Tup1, negative regulators of hyphal formation, were overexpressed in presence of capric or caprylic acid. Cell cycle studies revealed that capric and caprylic acids arrested cell cycle at G2/M and S phase. Targeting the virulence factors like yeast-to-hyphal transition is efficacious for treatment of opportunistic fungal infections. This research suggests that both capric and caprylic acid may be effective interventions for treating C. albicans yeast infections.

A Novel Approach for Object Detection in VHR Images

Ashwini Kunte,Siddhesh Shirodker,Rakesh Menaria,Bhavesh Patel 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.4

Spherical crystallization of ezetimibe for improvement in physicochemical and micromeritic properties

Ashwini Patil,Yogesh Pore,Yogesh Gavhane,Shitalkumar Patil,Sachinkumar Patil 한국약제학회 2014 Journal of Pharmaceutical Investigation Vol.44 No.3

Spherical agglomerates of ezetimibe (EZT)were prepared with hydrophilic polymers; polyvinyl pyrrolidoneK30 (PVP) and/or poloxamer 188 (poloxamer) atdrug to polymer ratios of 1:1 (w/w) by spherical crystallizationtechnique, in order to improve its physicochemicaland micromeritic properties. Three different bridging liquids;chloroform, dichloromethane and/or ethyl acetatealong with good solvent acetone and poor solvent waterwere used to form six batches of agglomerates. Initialcharacterization of all batches in terms of micromeritic andphysicochemical properties resulted in optimization of (A3,EZT:PVP:ethyl acetate) and (B3, EZT:poloxamer:ethylacetate) batches and hence further investigated for drug–polymer interaction, crystallinity and morphology usingFTIR, XRPD, DSC and SEM techniques. The resultsindicated presence of hydrogen bonding, crystallinity andspherical shape in agglomerates. Therefore, the optimizedagglomerates (B3) were directly compressed into tablet. Unfortunately, drug release from the tablet was not satisfactory,suggesting a need of disintegrant from dissolutionpoint of view. Therefore, these agglomerates were recompressedincorporating certain excipients and evaluated asper pharmacopoeia. The dissolution rate of prepared tabletwas similar to that of marketed tablet (p[0.05). It couldbe concluded that spherical crystallization could be one ofthe effective and alternative approaches for improvedperformance of EZT and its tablet formulation.

Weave Design Aspects of 3D Textile Preforms Towards Mechanical Properties of Their Composites

Ashwini Kumar Dash,Bijoya Kumar Behera 한국섬유공학회 2019 Fibers and polymers Vol.20 No.10

In this research, six different weave designs of 3D fabric preforms were produced which were subsequentlyconverted to their composite forms using compression molding technique. The composite flat panels thus produced werecharacterized with their response to in-plane tension, bending, and impact testing. The stuffer tows per layer to binder ratiowere kept 1:1 for all the samples. One of the preforms was produced without straight stuffer tows in order to categorize theirrole to different mechanical properties under this study. The tensile performance was found to be affected by the lineardensities of the load carrying fibres and also to the distribution of resin rich regions, determined by weave design of thepreforms. The flexural analysis revealed that the binder tows did not straighten and induce cracks like tensile properties,instead, they acted as a truss and improved bending property. Interlock weave designs showed better flexural properties thancorresponding orthogonal weave designs. The impact properties were predominantly influenced by the number of crossoverpoints in the fabric structure.

Structural Characterization and Functional Evaluation of an Exopolysaccharide Produced by Weissella confusa AJ53, an Isolate from Fermented Uttapam Batter Supplemented with Piper betle L. Leaves

Ashwini Kumar Dubey,Kadirvelu Jeevaratnam 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Weissella confusa AJ53 isolated from fermented Uttapam batter supplemented with Piper betle L. leaves produced an exopolysaccharide (EPS) from sucrose. The optimum culture conditions were determined for maximum production. EPS AJ53 was characterized using spectral analysis as an unbranched linear (α-1→6 linked) dextran, homopolymer of D-glucose. Melting and degradation temperatures of EPS AJ53 were 103.84 and 265℃, respectively. EPS AJ53 exhibited antioxidant activities for scavenging of hydroxyl, DPPH, and nitric oxide radicals, and metal ion chelating and poor reducing abilities. A fat binding ability, oil emulsifying activity, and syneresis-prevention activity due to a water holding capacity were demonstrated. Application as an adjunct to provide value addition to food products is indicated. A potent flocculating ability enables EPS AJ53 to be used for water treatment and cryoprotectant applications as an alternative cryoprotective agent. The yield and properties of EPS AJ53 indicate multiple uses.

Implementation of IoT Application using Geofencing Technology for Protecting Crops from Wild Animals

Ashwini L. Kadam,이훈,황민태 사단법인 미래융합기술연구학회 2020 아시아태평양융합연구교류논문지 Vol.6 No.6

In this paper, we propose a wild animal intrusion notification system using IoT and geofencing technology to protect crops from intruders such as wild animals. The system consists of an ultrasonic sensor to detect wild animals, LTE network communication for transmitting information about detection to a server system, geofencing technology that provides location information, and a mobile application that sends notifications to farmers in real-time. In this study, the prototype of the system was developed, and lab trials were carried out to analyze the time response performance between the hardware module and the mobile application used to receive the notification. It can be assumed that there will be no crop losses in farm fields if such a system is implemented. In the future, the system can also include wild animal intrusion prevention and prediction functions through big data analysis.

Understanding planform dynamics of the Ganga River in eastern part of India

Ashwini Kumar,Pathan Shehnaj Ahmed,Singh Ajai 대한공간정보학회 2021 Spatial Information Research Vol.29 No.4

To understand the natural processes of planform change, meandering and formation of braid bars, satellite sensor data of few decades play a significant role. The present research focuses on the planform dynamics of Ganga River from Sahibganj (Jharkhand) to Jalangi in West Bengal, India. This study is based on the sequential river planform maps. These maps were prepared using Landsat 1, Landsat 3, Landsat 4, Landsat 5 and Landsat 7 data for the period 1975–2015 at an interval of 5 years. The sinuosity index, braiding index and spatial analysis of maps were used to assess and classify the river into straight, braided and meander categories. This was observed that braiding index is continuously increasing and the meandering index has decreased from 1.6 to 1.24 during 1975 to 2015. The results showed the changes in channel migration with time and space. From the analysis, it is observed that the factors causing shifting of the Ganga River in some areas are natural and anthropogenic. Landsat images were found to be effective in determining the meandering index, braiding index and planform change of a river.

Micropropagation and DNA Delivery Studies in Onion Cultivars of Bellary, CO3

Ashwini Malla,Balamurugan Srinivasan,Bala Murugan Shanmugaraj,Sathishkumar Ramalingam 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.1

Onion is one of the most important vegetables grown all over the world. In the present study, rapidly growing and maintainable callus was accomplished using the basal meristem plate and twin scale leaves as explants in Bellary and CO3 varieties for callus induction and in vitro bulb formation. The two cultivars were transformed with Agrobacterium tumefaciens EHA 105 harboring the binary vector pCAMBIA 1301 containing the GUS gene with different concentrations of acetosyringone. The outcome of this study revealed that the frequency of callus induction was maximum on Murashige and Skoog medium (MS) fortified with B5 vitamins supplemented with 0.5 and 1 mg L-1 picloram. Regeneration of plantlets from the callus was observed on MS supplemented with 0.5 mg L-1 each of BAP and KIN and 0.1 mg L-1 NAA. In vitro bulb response was observed on MS with B5 vitamins supplemented with 2.0 mg L-1 BAP. GUS gene integration in the transgenic plants was confirmed by PCR. The maximum GUS gene expression was observed at a concentration of 150 μM acetosyringone in the transformed plants by histochemical assay. This protocol allows effective genetic transformation and regeneration system of onion via in vitro bulb development.

On the Alpha-Carbon-12 Elastic Scattering

Ashwini Kumar Behera,Ujjwal Laha,Madhura Majumder,Jhasaketan Bhoi 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.5

A closed form expression for the Fredholm determinant is constructed for motion in Coulomb-distorted nonlocal separable potential and expressed it in its maximal reduced form. We demonstrate the usefulness of our constructed expression by means of a model calculation.

Development of the Full Package of Gyrotron Simulation Code

Ashwini Sawant,EunMi Choi 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.11

A complete code-package for gyrotron simulation to analyze its performance is under development in UNIST, Korea. We first time report the present status of the code-package named as UNIST Gyrotron Design Tool (UGDT). It can perform design simulations for gyrotron's interaction cavity, RF window, and the essential mode calculations including the study of mode competition. We will discuss about its salient features, theory, numerical implementation, and its calculation result for 95 GHz UNIST Gyrotron. Moreover, we will validate its capability to perform the mode competition calculation for fundamental and second harmonic modes.