The Storm of Violence, the Surge of Struggle

Kristine Aquino VALERIO 이화여자대학교 아시아여성학센터 2014 이화여자대학교 아시아여성학센터 학술대회자료집 Vol.2014 No.1

Characterization of the Phenol Monooxygenase Gene from Chromobacterium violaceum: Potential Use for Phenol Biodegradation

Elen Aquino Perpetuo,Regina Célia Pereira Marques,Maria Anita Mendes,Wanessa Cristina de Lima,Carlos Frederico Martins Menck,Claudio Augusto Oller do Nascimento 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6

In this work, the biodegradation mechanism of phenol and sub products (such as catechol and hydroquinone) in Chromobacterium coli was investigated by cloning and molecular characterization of a phenol monooxygenase gene in Escherichia coli. This gene (Cvmp) is very similar (74 and 59% of similarity and identity, respectively) to the ortholog from Ralstomia eutropha, bacteria capable of utilizing phenol as the sole carbon source. The phenol biodegradation ability of E.coli recombinant strains was tested by cell-growth in a minimal medium containing phenol as the sole source of carbon and release of intermediary metabolites (catechol and hydroquinone). Interestingly, during the growth of these strains on phenol, catechol, and hydroquinone accumulated transiently in the medium. These metabolites were further analyzed by HPLC. These results indicated that phenol can be initially orto or para hydroxylated to produce cathecol or hydroquinone, respectively, followed by meta-cleavage of aromatic rings. To verify this information, the metabolites obtained from HPLC were submitted to LC/MS to confirm their chemical structure, thereby indicating that the recombinant strains utilize two different routes simultaneously, leading to different ring-fission substrates for the metabolism of phenol In this work, the biodegradation mechanism of phenol and sub products (such as catechol and hydroquinone) in Chromobacterium coli was investigated by cloning and molecular characterization of a phenol monooxygenase gene in Escherichia coli. This gene (Cvmp) is very similar (74 and 59% of similarity and identity, respectively) to the ortholog from Ralstomia eutropha, bacteria capable of utilizing phenol as the sole carbon source. The phenol biodegradation ability of E.coli recombinant strains was tested by cell-growth in a minimal medium containing phenol as the sole source of carbon and release of intermediary metabolites (catechol and hydroquinone). Interestingly, during the growth of these strains on phenol, catechol, and hydroquinone accumulated transiently in the medium. These metabolites were further analyzed by HPLC. These results indicated that phenol can be initially orto or para hydroxylated to produce cathecol or hydroquinone, respectively, followed by meta-cleavage of aromatic rings. To verify this information, the metabolites obtained from HPLC were submitted to LC/MS to confirm their chemical structure, thereby indicating that the recombinant strains utilize two different routes simultaneously, leading to different ring-fission substrates for the metabolism of phenol

LIFESTYLE YOUTUBE INFLUENCERS: CURATORS OF LIFESTYLE BRANDING

Elaina Aquino,Kiseol Yang,Lynn Brandon 글로벌지식마케팅경영학회 2020 Global Marketing Conference Vol.2020 No.11

Characterization of tannin extracts derived from the bark of four tree species by HPLC and FTIR

Ruiz-Aquino Faustino,Feria-Reyes Rossy,Rutiaga-Quiñones José Guadalupe,Robledo-Taboada Luis Humberto,Gabriel-Parra Rosalío 한국산림과학회 2023 Forest Science And Technology Vol.19 No.1

The objective of this work was the extraction and quantification of tannins obtained from the bark of four tree species from the forests of Ixtlan de Juarez, Oaxaca, Mexico (Arbutus xalapensis Kunth., Prunus serotina Ehrh., Quercus crassifolia Humb. and Bonpl., and Quercus laurina Humb. and Bonpl.), In this extraction process, 90% ethanol was used as solvent at an extraction temperature of 70 C for 2 h. The quantification of phenolic compounds, condensed tannins, and percentage of total extract, were obtained using the Stiasny number. The total extracts were 12.87, 16.38, 19.31, and 25.68% for P. serotina, Q. laurina, Q. crassifolia, and A. xalapensis, respectively. The condensed tannins were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) where at 1626 cm1 is observed an isomerization that implies a rotation between the C and B rings that corresponding at elongation of bonds in benzene structure of catechin. The purity of the extracted tannins was analyzed by High Performance Liquid Chromatography (HPLC). The effectiveness of the extraction process was proven; the importance of knowing the amount of tannins will promote the utilization of the bark of these species. The results of the condensed tannin content place Quercus crasifolia (10.29%) and Arbutus xalapensis (13.12%) as potential sources of tannins.

Implementation of the modified compression field theory in a tangent stiffness-based finite element formulation

Wilkins Aquino,Ibrahim Erdem 국제구조공학회 2007 Steel and Composite Structures, An International J Vol.7 No.4

A finite element implementation of the modified compression field theory (MCFT) using a tangential formulation is presented in this work. Previous work reported on implementations of MCFT has concentrated mainly on secant formulations. This work describes details of the implementation of a modular algorithmic structure of a reinforced concrete constitutive model in nonlinear finite element schemes that use a Jacobian matrix in the solution of the nonlinear system of algebraic equations. The implementation was verified and validated using experimental and analytical data reported in the literature. The developed algorithm, which converges accurately and quickly, can be easily implemented in any finite element code.

Food Cultural Landscapes: Toward the Valorization of Living Gastronomical Heritage

Raymond Aquino Macapagal 한국식생활문화학회 2017 한국식생활문화학회학술대회논문집 Vol.2017 No.11

Storm of Violence, Surge of Struggle: Women in the Aftermath of Typhoon Haiyan (Yolanda)

Kristine Aquino VALERIO 이화여자대학교 아시아여성학센터 2014 Asian Journal of Women's Studies(AJWS) Vol.10 No.1

Typhoon Haiyan, known locally as Yolanda, lashed the Philippines on November 8, 2013 affecting more than 10 percent of the country’s population. Although exploitation of the environment and unequal distribution of wealth in disaster situations are widely discussed among scholars, activists, humanitarian workers, and public intellectuals, issues pertaining to women and girls must be addressed as important as well. Thus, this paper problematizes the impact of typhoon Haiyan in the lives of women and girl survivors. It sees the effect of this calamity on the sexual and reproductive health and rights (SRHR) of women and girls and how further marginalization of women and girls during disasters increases their vulnerability to sexual and gender-based violence (SGBV). Making such an analysis, this paper responds to the call for solidarity among women, which will enable the demand for the fullest attainment of the SRHR needs of women and girls and the elimination of SGBV.

What is a traditional food? Conceptual evolution from four dimensions

Rocillo-Aquino Zeltzin,Cervantes-Escoto Fernando,Leos-Rodríguez Juan Antonio,Cruz-Delgado Daniela,Espinoza-Ortega Angélica 한국식품연구원 2021 Journal of Ethnic Foods Vol.8 No.-

The purpose was to specify the concept of traditional food and the dimensions that make it up, identifying: definitions, authors and research projects; as well as to determine what is known and possible topics for future research. A literature review of traditional foods was conducted that examined the conceptual development of the term. Social network analysis (SNA) was also used to identify the most relevant definitions and working groups on the topic. Twenty-three definitions were identified in the period 1995–2019. It reveals the difficulty of establishing one that ecompasses such a dynamic concept. Although there is variability in the specific characteristics of these foods, four dimensions have been established: time, place, know-how, and cultural meaning. It was found that their main characteristic is the transmission of knowledge and raw materials between generations. The conceptualization of the term has been developed mainly in Europe, based on the perspective of consumers. New trends in research include the contrast and complementarity of innovation in traditional foods and the difference between these products and those named with similar attributes such as typical, regional, ethnic, local, among others. Social network analysis (SNA) was used to study the definitions of a concept, something that had not been done with this approach. Suggestions are made for possible research on the subject, such as the conceptual delimitation of related terms and the compatibility between innovation and tradition. The purpose was to specify the concept of traditional food and the dimensions that make it up, identifying: definitions, authors and research projects; as well as to determine what is known and possible topics for future research. A literature review of traditional foods was conducted that examined the conceptual development of the term. Social network analysis (SNA) was also used to identify the most relevant definitions and working groups on the topic. Twenty-three definitions were identified in the period 1995–2019. It reveals the difficulty of establishing one that encompasses such a dynamic concept. Although there is variability in the specific characteristics of these foods, four dimensions have been established: time, place, know-how, and cultural meaning. It was found that their main characteristic is the transmission of knowledge and raw materials between generations. The conceptualization of the term has been developed mainly in Europe, based on the perspective of consumers. New trends in research include the contrast and complementarity of innovation in traditional foods and the difference between these products and those named with similar attributes such as typical, regional, ethnic, local, among others. Social network analysis (SNA) was used to study the definitions of a concept, something that had not been done with this approach. Suggestions are made for possible research on the subject, such as the conceptual delimitation of related terms and the compatibility between innovation and tradition.

The Factors that Lead to Inpatient Falls

Alison R Aquino 한국간호과학회 2011 한국간호과학회 학술대회 Vol.2011 No.10

Lived Experiences during the Enhanced Community Quarantine : Students’ Perspectives through Value Judgment Lenses

Avelina M. Aquino,Florinda G. Vigonte, MAE,Enrique A. De Jesus, MBS ASCONS 2020 INTERNATIONAL JOURNAL OF EMERGING MULTIDISCIPLINAR Vol.4 No.3

The spread of Corona virus (COVID-19) is pandemic. As the congress enacted the Bayanihan: to Heal as One Act, officially designated as Republic Act No. 11469, the president implemented the enhanced community quarantine (ECQ) in Luzon. Due to ECQ, there is the cessation of work, business, schooling and transportation; so people just stay at home to avoid contagion. It was in this contention that the researchers conducted this study to explore and navigate the thoughts and perspectives of the 15 graduating college students about their lived experiences during the ECQ. The only instrument employed in this research was the closed-fixed interview that covered both factual and meaning-generation level. There were no predetermined questions asked, in order to remain as open and adaptable as possible to the participants’ thoughts and perspectives on the enhanced community quarantine thru their value judgment lenses. The researchers prepared interview questions; participants were asked thru phone calls and skype. Responses were analyzed and categorized to extract their thoughts and perspectives. Conclusions were drawn and future research directions were offered.