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韓宗鉉,白承和,康成溶,宋昊埈 한국전통의학연구소 1997 한국전통의학지 Vol.7 No.2
시중에 유통되고 있는 4종류의 감초(특감초,원감초,일호감초,이호감초)에 대하여 유효약리성분 glycyrrhizin, 18α-glycyrrhetinic acid 및 glycyrrhizic ammonium salt의 함량을 대한약전의 규격기준에 준하여 검토한 결과는 다음과 같았다. 1. 감초의 수분함량은 7.00-10.16%범위 였다. 2. 감초의 회분 및 산불용성 회분 함량은 3.03-4.09%, 0.02-0.11 % 범위 였다. 3. 감초 품질의 지표성분인 glycyrrhizin,18α-glycyrrhetinic acid 및 glycyrrhizic ammonium sait 을 TLC로 각각 Rt값 0.399,0.932,0.394 위치에서 암자색의 반점을 확인할 수 있었는데 특 감초,원 감초,1호 감초,2호 감초 모두 같은 위치에서 뚜렷한 암자색 반점을 확인할 수 있었고,확인되지 않은 3종 화합 물의 Rt 값은 0.28,0.66,0.78 였다. 4. 감초품질의 지표성분인 glycyrrhizin,18 α-glycyrrhetinic acid 및 glycyrrhizic ammonium salt을 HPLC로 정량한 결과 glycyrrhizin은 4종류 감초에서 분석되어 2.93-4.48g% 범위였고, 18 α-glycyrrhetinic acid는 특감초 및 일호감초에서만 13.64,3.52 g% 함유되었다. 그러나 glycyrrhizic ammonium salt 분석되지 않았다. 5. 문헌상에 기록된 감초의 종류는 16종이었고 이들에서 분리된 화학물질은 약 170여 가지였다. This study was carried out to investigate the content of glycyrrhizin, 18 α-glycyrrhetinic acid, glycyrrhizic ammonium salt, moisture, ash, residue ash in four kinds(teuk-gamcho, won-gamcho, IIho-gamcho, Eho-gamcho) in Glycyrrhizae Radix(Glycyrrhiza sp.) used in Korea. The result were as follows ; The moisture content in four kinds of gamcho was ranged from 7.00-10.16%. The ash and ash of acid residue in all gamcho types ranged from 3.03-4.09% and from 0.02-0.11 %, respectivly. The glycyrrhizin, 18 α-glycyrrhetinic acid, glycyrrhizic ammonium salt in Teuk-gamcho, Won-gamcho, IIhogamcho, Eho-gamcho verified from line of dark purplue color by TLC and Rf value of glycyrrhizin, 18 α-glycyrrhetinic acid, glycyrrhizic ammonium salt were 0.399, 0.932, O. 394, respectivly, and the Rf value unknown compoud were 0.28, 0.66, 0.78. The glycyrrhizin, 18 α-glycyrrhetinic acid, glycyrrhizic ammonium salt content of all gamcho by HPLC were ranged from 2.93-4.48g%, 3.52-13.64g%, and trace, respectively. The 16 kinds of Glycyrrhiza (Licorice) were dassified, and that the identification about 170 chemicals were phamacology action substrate by reported references.
수종(數種) 한약재(韓藥材)가 기관지평골근(氣管支平滑筋)에 미치는 영향(影響)(I)
한종현,송호준,강성용,김공수,오광수,Han, Jong-Hyun,Song, Ho-Jun,Kang, Sung-Yong,Kim, Gong-Soo,Oh, Kwang-Soo 대한한방내과학회 1996 大韓韓方內科學會誌 Vol.17 No.1
Several medicinal Plants in Jeonbuk are screened for the contraction or relaxation to the acetylcholine and histamine induced contraction in the isolated rat and guinea pig's trachea smooth muscle. Contractions evoked by acetylcholine and histamine were inhibited by Flos Farfarae, Poria and Rhizoma alismatis, but contractions were increased Tuber Pinelliae, Herba Chelidonii, Fructus Qusqualis, Radix Asari, Semen Perillae, Folium Artemisiae and Fructus Schizamdrae increased the contractions evoked by acetylcholine and histamine. Radix Puerariae, Radix Osterici Koreani, Rhizoma Zingiberis siccatum, Radix Sanguisorbae, Rhizoma Atractylodis, Fructus gardeniae and Cortex Magnoliae did not effect the contractions evoked by acetylcholine and histamine.
관동화전탕액(款冬花煎湯液)이 기관지평골근(氣管支平滑筋)에 미치는 영향(影響)
한종현,강성용,유광석,진상식,하경화,이경자,Han, Jong-Hyun,Kang, Sung-Yong,Yu, Kwang-Suk,Jin, Sang-Sik,Ha, Kyung-Hwa,Lee, Kyung-Ja 대한한방내과학회 1996 大韓韓方內科學會誌 Vol.17 No.1
Farfarae Flos, a traditional herb medicine, has been used in Korea and China for many centuries as a treatment for respiratory disease. The purpose of the present study was to determine the effect of Farfarae Flos on histamine-induced tracheal smooth muscle contraction in rats. Guinea pigs(500g, female) were killed by CO2 exposure and a segment (8-10mm) of the thoracic trachea from each guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine which evoked 50% of maximal response (ED50) was obtained from cumulative dose response curves for histamine (10-7-10-4M). Contractions evoked by histamine(ED50) were inhibited significantly by Farfarae Flos. The mean percent inhibition was 8.7% after 1.5mg/ml Farfarae Flos, and 33.5% (p<0.05) after 5.0mg/ml Farfarae Flos. Propranolol (10-7M) slightly but significantly attenuated the inhibitory effects of Farfarae Flos. Following treatment with propranolol, the mean percent inhibition caused by 5.0mg/ml Farfarae Flos. Indomethacin and methylene blue (10-7M) did not significantly alter the inhibitory effect of Farfarae Flos. These results indicate that Farfarae Flos can relax histamine-induced contraction of guinea pig tracheal smooth muscle, and that this inhibition involves, in part, symphathetic nerve system.