동일초점현미경을 이용한 비쇼그렌건성안 각막 미세구조의 변화 및 임상증상과의 관계

최은영(Eun Young Choi),김태임(Tae Im Kim),서경률(Kyoung Yul Seo),김응권(Eung Kweon Kim),이형근(Hyung Keun Lee) 대한안과학회 2015 대한안과학회지 Vol.56 No.5

목적: 동일초점현미경을 이용해 비쇼그렌건성안 환자에서 관찰되는 각막 상피세포와 기저하신경의 변화와 임상 증상과의 관계를 알아본다. 대상과 방법: 건성안 환자 40명과 정상인 18명이 연구에 참여했다. 설문을 통해 건성안 증상을 visual analogue scale (VAS) 통증지수로 평가해서 0점에서 5점을 낮은 통증지수군(low VAS score group, LVS), 6점에서 10점을 높은 통증지수군(high VAS score group, HVS)으로 구분했다. 모든 피험자의 양안을 대상으로 쉬르머 검사, 눈물막파괴시간 및 각막염색점수 검사를 시행한 후, 동일초점현미경을 이용해 각막 중심부에서 상피층과 기저하신경의 미세구조를 촬영했다. 정상안 23안과 건성안 54안이 분석에 포함되었다. ImageJ와 NeuronJ 프로그램을 이용해 세포의 밀도와 형태학적 특징을 분석했다. 결과: 대조군에 비해 두 건성안군에서 표층, 중간 및 기저상피세포의 밀도가 유의하게 감소해 있었다(p<0.001). 중간상피세포는 증상이 심한 건성안군에서 보다 낮은 밀도로 관찰되었다(p<0.0001). 기저하신경의 밀도는 높은 통증지수군에서 대조군 및 낮은 통증지수군에 비해 낮았고(p<0.005), 신경의 염주화, 비틀림 정도 및 반사성은 높은 통증지수군에서 대조군 및 낮은 통증지수군보다 높게 나타났다(p<0.05). 결론: 동일초점현미경으로 분석한 건성안 환자 각막의 세포 수준의 변화는 건성안의 병리 및 임상증상과 연관성이 있다. 이는 건성안환자의 진단 및 치료 반응 평가에 보다 객관적인 지표가 될 수 있다. <대한안과학회지 2015;56(5):680-686> Purpose: To investigate the relationship between changes of corneal epithelium and subbasal nerves in non-Sjögren dry eye us-ing in vivo confocal microscope (IVCM) and self-reported clinical symptoms. Methods: The present study included 40 patients with dry eye and 18 healthy control subjects. The dry eye group underwent an evaluation of dry eye symptoms using visual analogue scale (VAS) score and was subdivided into 2 groups; score 0-5 as the low VAS score (LVS) group and score 6 - 10 as the high VAS score (HVS) group. The tear film break-up time, fluorescein staining, Schirmer test and microstructural imaging of epithelium, and subbasal nerve at cornea center with IVCM were performed on both eyes of each patient. Twenty-three normal eyes and 54 eyes of dry eye patients were included in the study. Cell densities and morphological characteristics were analyzed using ImageJ and NeuronJ softwares. Results: Both LVS and HVS groups had decreased cell density of superficial, intermediate, and basal epithelium (p < 0.001). Intermediate epithelial cells were more decreased in the dry eye group with more severe symptoms (p < 0.0001). Subbasal nerve density (p < 0.005) was more decreased and nerve beadings, tortuosity, and reflectivity increased in the HVS group than both LVS and control groups (p < 0.05). Conclusions: The alterations of corneal cellular level in dry eye patients visualized using IVCM are correlated with pathology and clinical symptoms and may be useful objective criteria in diagnosis and monitoring treatment efficacy. J Korean Ophthalmol Soc 2015;56(5):680-686

신경외과 수술로 인한 신경영양각막염 환자에서 동일초점현미경을 이용한 각막미세구조 변화 분석

이동현*,최은영*,김응권,이형근,Dong Hyun Lee*,Eun Young Choi*,Eung Kweon Kim,Hyung Keun Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.12

Purpose: To investigate the changes of the corneal microstructure of neurosurgically-induced neurotrophic keratitis patients compared to normal human corneas using in vivo confocal microscope (IVCM). Methods: Ten eyes in the normal control group and 11 eyes in the neurosurgically-induced neurotrophic keratitis patient group were included in the present study. After corneal sensitivity tests were performed, thickness of each layer and number of endothelial cells and stromal keratocytes in the cornea were assessed using IVCM. Morphological characteristics of the corneal nerves were measured by ImageJ software. Results: After analysis of corneal thickness layer by layer, the Bowman’s layer was significantly reduced in the neurosurgicallyinduced neurotrophic keratitis patient group compared with the normal control group (<EM>p</EM> = 0.016) and the portion of Bowman’s layer was significantly reduced in the neurosurgically-induced neurotrophic keratitis patient group compared with the control group (<EM>p</EM> = 0.024). The nerve fiber length per square-millimeter became significantly shorter compared to the normal control group (<EM>p </EM>= 0.042). The nerve fiber length per square millimeter showed significant correlations with the number of fibers, number of beading, branching patterns, and nerve tortuosity (<EM>p</EM> = 0.002, 0.002, 0.013 and 0.034, respectively). The number of endothelial cells and stromal keratocytes, the number of nerve fibers and beading, and the pattern of branching and nerve tortuosity showed no significant differences between the normal and neurosurgically-induced neurotrophic keratitis patient groups. Conclusions: Our results showed that decreased thickness of Bowman’s layer may be related to the decreased corneal nerve distribution, secondary to the dysfunction of trigeminal nerve branch innervating the cornea. The microstructural changes of Bowman’s layer can help diagnose the disease and evaluate the current status in neurosurgically-induced neurotrophic keratitis patients. J Korean Ophthalmol Soc 2014;55(12):1765-1771

필드하키 경기 시간 구조 및 특성 분석

김지응(Kim, Ji-Eung),이승훈(Lee, Seung-Hun),최은영(Choi, Eun-Young),박종철(Park, Jong-Chul) 한국체육과학회 2021 한국체육과학회지 Vol.30 No.5

The purpose of this study is to identify the feature of the Actual Playing Time (APT) and Duration of Out of play (DOP) between the national women’s hockey team and opponents team and identify the different factors in international games. The data was collected from 6 A matches in the World Finals held in 2017, and to confirm the difference between the two groups, t-verification was performed using SPSS 25.0. As a result, first, the APT of the national women’s hockey team was an average of 17 minutes and 15 seconds per game. On the other hand, the APT of the opponent was 23 minutes and 35 seconds. Second, the DOP of the national women’s hockey team was an average of 10 minutes 8 seconds, and the DOP of opponents was an average of 8 minutes 37 seconds. Third, there were significant differences in foul, Outlet, long-corner, and side-line situations during off-time. according to these results, combining time management operations with various tactical training in training situations will help improve women’s hockey performance.

간호직 근로자의 근골격계질환 및 직무스트레스 위험요인 : 종합병원 간 호작업 및 근골격계질환 증상

박정근(Jung-Keun Park),장승희(Seung-Hee Jang),김대성(Day-Sung Kim),허경화(Kyung-Hwa Hur),이혜영(Hea-Young Lee),최은영(Eung-Young Choi),조준희(Joon-Hee Cho),우희성(Hee-Sung Woo) 대한인간공학회 2010 대한인간공학회 학술대회논문집 Vol.2010 No.10

간호사의 근골격계질환(musculoskeletal disorders, MSDs) 및 직무스트레스 문제는 지속적으로 관심을 보여 왔는데 간호 업무 또는 작업을 이해하여 위험요인을 파악하는 노력은 부족했다. 본 연구는 종합병원 간호사를 대상으로 실시한 설문조사 자료를 바탕으로 간호사 업무에 대해 작업을 분석하고 MSD 증상호소의 실태를 살펴봄으로써 간호사의 주요 작업과 MSD 증상의 특징을 이해하고자 했다.

Characterization of Clones of Human Cell Line Infected With Porcine Endogenous Retrovirus (PERV) from Pocine Cell Line, PK-15

Kim, Jung Heon,Choi, Eun young,Jung, Eun-Suk,Kwon, Yejin,Lee, Dong Suk,Hwang, Do Yeong,Hwang, Eung Soo 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Porcine endogenous retroviruses (PERVs) form part of the chromosomes of all pigs. Since they can be produced as infectious virion and infect human cells, safety issues on PERVs infection to human are still controversial and is one of main hurdles of xenotransplantation using pig cells or organs, It has been reported that the established porcine cell line, PK-15, produces PERVs and can infect the human cell lines. Therefore, clonal analysis on human cell line infected with PERV is a prerequisite to characterize the infectivity to human cells and to investigate the harmfulness of PERVs to human. Materials and Methods : For the characterization of PERV that originates from porcine cell line, PK-15, full length PERV cloning from genomic DNA of PK-15 was performed and partial sequences of both ends were achieved. Cell clones from human cell line, 293, persistently infected with PERVs from PK-15 were established by the method of limiting dilution. Nested PCR and direct sequencing of PCR products in each clone were carried out so as to confirm the PERV genomes in each clone. The growth rate of each clone was checked using cell counting and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the infectivity by reverse transcriptase (RT) assay, and genetic analysis by karyotyping. Results : A total of 12 genomic PERV clones could be retrieved; 1 with full length, 4 with defective forms, and others with irrelevant sequences, Intact PERV was thought to be able to infect 293 and the PERV-infected cell clones were selected by limiting dilution. PCR results confirmed that nine cell clones were infected with PERV, and sequence alignment data on PCR products of pol region from PK-15 and human cell clones with PERV showed very similar results. Cell counting and MTT assay for growth kinetics of each clone indicated that two clones showed reduced growth rate. However, it was difficult to verify the effect of PERV infection on the cell growth because of the presence of many genetic alterations in 293 parental cells. No RT activities were detected in the culture supernatant from PERV-infected 293 cell clones. Conclusion : The sequences of PERVs were detected in human cell clones after PERV infection, but PERV virions could not be detected from the culture supernatant by RT assay.