돼지 Lecithin : Cholesterol Acyltransferase 의 화학적 성질

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1

Amino acid residues of lecithin: cholesterol acyltransferase from hog plasma were modified with various chemicals in order to identify the functionally important amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentrations in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipopotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophobic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indicating an importance of those amino acid residues in the function of the enzyme.

한의약학의 서지학적(書誌學的) 연구(I) -상한론(傷寒論)을 중심으로-

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2006 약학회지 Vol.50 No.4

Three important books in the oriental medicine are Huangjenaekyung (medical book), Sanghanron (pharmacological book) and Shinnongbonchokyung (herbal book). Chang, joong-kyung who is the writer of Sanghanron would be recommended as a saint physician and his book, Sanghanron is granted for a best book in this field by Chinese people. Also, Japanese physicians would estimate Sanghanron as the most magnificent book ever written by a human being. Sanghanron contains a lot of confirmations (證) of diseases, their progressing courses and exact therapeutic ways. Therefore modern physicians and pharmacists who are interested in the oriental medicine might study and practice more scientific approach of therapy for patients. The aim of this bibliographical study on Sanghanron is to explain systematically theories of this book and harmonize them with the modern medicinal study: In this study we have acquired three main results, the first is that we could get the coincidence between western & oriental way on the five therapeutic principles-sweating, vomiting, harmonizing, excreting and bleeding : the second, notice the exclusion of the Five Element Theory(the fundamental one in oriental medicine) and the third, found out the possibility of the immunological and neuroscientific explanation for pharmacological application to the oriental medicine.

한의약학(韓醫藥學)의 서지학적(書誌學的) 연구(II)-[신상한론(新傷寒論)] - 노장사상(老莊思想)에 의한 한의약학의 과학적 통합이론 -

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2007 약학회지 Vol.51 No.1

Traditional Medicine (TM) is called as philosophical medicine in Korea. An unified theory named as Sihnsang-hanron (SSHR) was hypothesized through studies of scientific analyses on various theories of TM. SSHR has extracted seven concepts which are six common ones from the great three books (三大原典) and the Logic of Laotzu & Chuangtzu (老莊思想). Six common concepts are the affecting by cold (傷寒), qi (氣), cold or heat (寒熱), exterior & interior of body (表裏), deficiency or excessiveness (虛實), and yin & yang (陰陽). We have tried to apply these seven concepts to Physics and Life Science. The affecting by cold means anti-sunlight and the origin of all diseases. The difference between TM and modern medical science would be in diagnostic methods as well as their theoretical analyses for various diseases. The modern science follows Haeckel's positive dialectics applied by the biological monism, and oriental one(SSHR) does Yin-Yang monism from the studies of Logic of Laotzu (老子) & Chuangtzu (莊子). SSHR would make the theory of exterior & interior of body (表裏論) and six channels (六經) develope scientifically as a diagnostic technique of disease. This theory is an excellent one that can't be found out in modern medical science, and so it should be developed as a scientific theory by using modern mechanic instrument. Chuangtzu asserted that ai was the basic substance of the universe. It is hypothesized that qi (氣) is like small particles -higgs, with dynamic power in modern Physics. We consider cautiously qi could be calculated by mathematics through higgs' bosons in near future.

Effects of Human Breast Milk and Hyperlipidemic Serum on the Cholesterol Metabolism in Organ Culture of Fetal Aorta

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

출생 때에 토끼는 성숙한 토끼보다 비교적 높은 혈중 콜레스테롤을 포함하고 있으며 그 농도는 수유에 따라 증가하여 수유 2주일에 최대치를 보이다가 수유기간이 끝나는 4주째에는 정상농도로 돌아가게 된다. 사람의 모유 또는 와타나베토끼(유전적으로 혈중 콜레스테롤이 비정상적으로 높은 토끼)의 혈청으로부터 분리한 저밀도 리포단백질을 배지에 포함시켜 출생전 토끼의 대동맥을 조직 배양할 경우 대동액내에 포함되어 있는 콜레스테롤 양의 변화를 관찰하였다. 사람의 모유를 함유한 배지에 먼저 배양한 후 와타나베토끼의 저밀도 리포단백질을 함유한 배지로 옮겨 배양했을 때 출생전 토끼 대동맥내 콜레스테롤 농도는 사람의 모유에서만 같은 기간동안 배양한 대동맥에서와 비슷했다. 그러나 사람의 모유에서 먼저 배양하지 않고 와타나베토끼의 저밀도 리포단백질을 함유한 배지에서만 배양한 대동맥에서는 훨씬 높은 콜레스테롤 농도를 보여주었다. 이 결과로 미루어 보아 사람의 모유가 대동액내의 콜레스테롤 축척을 막아주는 역할을 한다는 사실을 추측할 수 있다. Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

사람의 모유가 대동맥내의 콜레스테롤대사에 미치는 영향

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

Effects of Human Breast Milk on Lipid Metabolism in Organ Culture of Fetal Rabbit Aorta

최명숙,박용복 ( Myung Sook Choi and Yong Bok Park ) 한국영양학회 1991 정기총회 및 심포지움 Vol.- No.26

Chemical Modifications of Lecithin: Cholesterol Acyltransferase from Hog Plasma

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1

Amino acid residues of lecithin:cholesterol acyltransferase form hog plasma were modified with various chemicals in order to identify the functionally important amino amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentration in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipoprotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophodic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indication an importance of those amino acid residues in the function of the enzyme. 돼지혈장으로부터 분리한 lecithin : cholesterol acyltransferase의 아미노산 잔기들을 여러 가지 화학물질로 변환시킴으로서 이 효소의 촉매기능에 중요한 역할을 담당하는 아미노산 잔기들을 규명하려 하였다. 최소한 한 개의 thiol기가 이 효소의 촉매반응에 필요하며 높은 효소농도에서는 네 개 모두의 thiol기가 분자간 disulfide 결합을 형성함으로서 효소분자간의 결합에 관여할 것으로 추정된다. 전체 tryptophan 잔기의 절반 이상이 효소분자의 표면에 노출되어 있으며 이러한 소수성 아미노산 잔기들이 이 효소의 기질인 high-density lipoprotein 표면의 apolipoprotein-AI과 소수성 결합에 관여함으로서 효소-기질 결합에 참여할 것으로 추정된다. 이 효소의 역가는 serine, thiol 그리고 histidine 잔기를 변환하는 화학물질에 의해 민감하게 저해됨으로서 이러한 아미노산 잔기들이 이 효소의 촉매반응에 관여함을 제시해주고 있다.

대학생의 성지식, 성태도, 성경험, 성적 자율성에 관한 연구

최명숙 ( Myung Sook Choi ),하나선 ( Na Sun Ha ) 한국여성건강간호학회 2004 여성건강간호학회지 Vol.10 No.4

대학생의 환경호르몬 노출 위험 행위와 우울, 불안, 스트레스의 관계

최명숙(Choi Myung Sook) 사단법인 안전문화포럼 2021 안전문화연구 Vol.- No.14

본 연구의 목적은 대학생의 환경호르몬에 대한 인식, 지식, 환경호르몬(내분비계 장애 물질) 노출 위험 행위와 정신건강 상태(우울, 불안, 스트레스)를 파악하고 그 관계를 확인하는데 있다. 본 연구의 대상자는 S시에 소재한 대학교에 재학 중인 대학생 243명으로 하였으며, 자료수집은 구조화된 설문지로 환경호르몬에 대한 인식과 지식, 환경호르몬 노출 위험 행위, 우울, 불안, 스트레스 척도-21(K-DASS-21)한글판을 사용하였다. 자료 분석은 IBM SPSS Statistics Ver 24를 이용하여, t-test와 ANOVA, Pearson correlation coefficient로 분석하였다. 연구 결과는 다음과 같다. 환경호르몬에 대한 인식 정도는 2.79점(점수 범위 1-5)으로 보통 정도인 것으로 나타났다.‘환경호르몬이 사람에게 위험하다고 생각한다’에는 평균 3.63점으로 점수가 가장 높았다. 연구 대상자의 환경호르몬에 대한 지식 정도는 총 30개 문항에 대한 정답률이 58.2%로 환경호르몬에 대한 지식수준이 높지 않은 것으로 나타났다. 환경호르몬 노출 위험 행위 점수는 2.78점(점수 범위 1∼5점)으로 보통 수준이었다. 대상자의 우울 평균 점수는 10.64점으로 가벼운 우울 상태, 불안 평균 점수는 8.72점으로 가벼운 불안, 스트레스 평균 점수는 12.01점으로 정상 범위였다. 환경호르몬 노출 위험 행위는 우울, 불안, 스트레스와 유의한 정적 상관관계가 있었다. 따라서 환경호르몬 노출 위험 행위를 많이 할수록 우울, 불안, 스트레스 증상을 많이 호소하는 것으로 나타났다. 환경호르몬 노출 위험 행위는 스트레스 정도 5단계에 따라 유의한 차이가 있었다. 우울, 불안의 5단계에 따른 환경호르몬 노출 위험 행위에는 유의한 차이가 없는 것으로 나타났으나, 항목별로 환경호르몬 노출 위험 행위에 차이가 있는 것으로 나타났다. 환경호르몬 노출 위험 행위에 따라 우울, 불안, 스트레스 증상에 차이가 있어, 환경호르몬 노출 위험 행위를 줄일 수 있는 프로그램개발 및 적용이 필요하다. The purpose of this study is to identify and confirm the relationship between university students awareness, knowledge, and risk behavior of exposure to environmental hormones (endocrine disruptors) and their mental health status (depression, anxiety, and stress). The subjects of this study were 243 university students enrolled at a university located in S city. Data collection was a structured questionnaire using awareness and knowledge of endocrine disruptors, risk behavior of exposure to endocrine disruptors, Korean version of depression, anxiety, and stress scale-21 (K-DASS-21). Data analysis was performed using IBM SPSS Statistics Ver 24, with t-test, ANOVA, and Pearson correlation coefficient. The study results are as follows. The degree of awareness of endocrine disruptors hormones was 2.79 points (score range of 1-5), which was found to be moderate. When asked, I think environmental hormones(endocrine disruptors) are dangerous to humans, the highest score was 3.63 out of 5. As for the degree of knowledge of endocrine disruptors of the study subjects, the correct answer rate for a total of 30 questions was 58.2%, and the level of knowledge of endocrine disruptors was not high. The environmental hormone(endocrine disruptors) exposure risk behavior score was 2.78 points (score range 1 to 5 points). The average score of the subject s depression was 10.64, indicating a mild depressive state, and the average score of anxiety was 8.72, and the average score of mild anxiety and stress was 12.01, which was within the normal range. Behaviors at risk of exposure to endocrine disruptors were significantly positively correlated with depression, anxiety, and stress. Therefore, it was found that the more risky behaviors exposed to endocrine disruptors, the more symptoms of depression, anxiety, and stress were reported. There was a significant difference in the risk behavior of exposure to endocrine disruptors according to the five levels of stress. There was no significant difference in the risk behavior of exposure to endocrine disruptors according to the five stages of depression and anxiety, but there was a difference in the risk behavior of exposure to environmental hormones by item. It is necessary to develop and apply a program to reduce the risk of exposure to endocrine disruptors, as the levels of depression, anxiety, and stress vary according to risky behaviors exposed to endocrine disruptors.

중국 신시기 ‘民族風’ 음악의 大衆화 연구

최명숙(Choi, Myung-Sook) 중국문화연구학회 2018 중국문화연구 Vol.0 No.41

After the Cultural Revolution, popular music from Hong Kong and Taiwan was introduced to the mainland through various media outlets and developed with an absolute impact on the industrialization, diversification, and commodification of native music. Moreover, with the introduction of a market economy and a political upheaval, the creation of music by mainland China, which had been suppressed for a long time, resumed again, bringing about new developments in the way music was created and the quality of music. In this issue, we will study the first generation composer, the people’s musician, and the singer of the time. Since the opening of the reform, it can be said that shiguangnan’s creative music has represented the achievements of the continental lyricism of the early New Year and the trend of the times. Most of his songs have the simple and intimate characteristics of private houses because they have been artistically processed by finding and applying the visitors of rich and beautiful minorities from various parts of China. In this study, the musical creation of shi-guangnan focuses on the musical characteristics of the “Minjufeng”, the most important phenomenon of Chinese culture, and on the folk music of Chinese minority Uighur people. It will also focus on looking at lyricism, which was popular in the mainland during the new era, and the phenomenon of nationalism in early days of pop music.