돼지 Lecithin : Cholesterol Acyltransferase 의 화학적 성질

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1

Amino acid residues of lecithin: cholesterol acyltransferase from hog plasma were modified with various chemicals in order to identify the functionally important amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentrations in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipopotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophobic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indicating an importance of those amino acid residues in the function of the enzyme.

한의약학의 서지학적(書誌學的) 연구(I) -상한론(傷寒論)을 중심으로-

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2006 약학회지 Vol.50 No.4

Three important books in the oriental medicine are Huangjenaekyung (medical book), Sanghanron (pharmacological book) and Shinnongbonchokyung (herbal book). Chang, joong-kyung who is the writer of Sanghanron would be recommended as a saint physician and his book, Sanghanron is granted for a best book in this field by Chinese people. Also, Japanese physicians would estimate Sanghanron as the most magnificent book ever written by a human being. Sanghanron contains a lot of confirmations (證) of diseases, their progressing courses and exact therapeutic ways. Therefore modern physicians and pharmacists who are interested in the oriental medicine might study and practice more scientific approach of therapy for patients. The aim of this bibliographical study on Sanghanron is to explain systematically theories of this book and harmonize them with the modern medicinal study: In this study we have acquired three main results, the first is that we could get the coincidence between western & oriental way on the five therapeutic principles-sweating, vomiting, harmonizing, excreting and bleeding : the second, notice the exclusion of the Five Element Theory(the fundamental one in oriental medicine) and the third, found out the possibility of the immunological and neuroscientific explanation for pharmacological application to the oriental medicine.

사람의 모유가 대동맥내의 콜레스테롤대사에 미치는 영향

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

한의약학(韓醫藥學)의 서지학적(書誌學的) 연구(II)-[신상한론(新傷寒論)] - 노장사상(老莊思想)에 의한 한의약학의 과학적 통합이론 -

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2007 약학회지 Vol.51 No.1

Traditional Medicine (TM) is called as philosophical medicine in Korea. An unified theory named as Sihnsang-hanron (SSHR) was hypothesized through studies of scientific analyses on various theories of TM. SSHR has extracted seven concepts which are six common ones from the great three books (三大原典) and the Logic of Laotzu & Chuangtzu (老莊思想). Six common concepts are the affecting by cold (傷寒), qi (氣), cold or heat (寒熱), exterior & interior of body (表裏), deficiency or excessiveness (虛實), and yin & yang (陰陽). We have tried to apply these seven concepts to Physics and Life Science. The affecting by cold means anti-sunlight and the origin of all diseases. The difference between TM and modern medical science would be in diagnostic methods as well as their theoretical analyses for various diseases. The modern science follows Haeckel's positive dialectics applied by the biological monism, and oriental one(SSHR) does Yin-Yang monism from the studies of Logic of Laotzu (老子) & Chuangtzu (莊子). SSHR would make the theory of exterior & interior of body (表裏論) and six channels (六經) develope scientifically as a diagnostic technique of disease. This theory is an excellent one that can't be found out in modern medical science, and so it should be developed as a scientific theory by using modern mechanic instrument. Chuangtzu asserted that ai was the basic substance of the universe. It is hypothesized that qi (氣) is like small particles -higgs, with dynamic power in modern Physics. We consider cautiously qi could be calculated by mathematics through higgs' bosons in near future.

Effects of Human Breast Milk on Lipid Metabolism in Organ Culture of Fetal Rabbit Aorta

최명숙,박용복 ( Myung Sook Choi and Yong Bok Park ) 한국영양학회 1991 정기총회 및 심포지움 Vol.- No.26

Chemical Modifications of Lecithin: Cholesterol Acyltransferase from Hog Plasma

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1

Amino acid residues of lecithin:cholesterol acyltransferase form hog plasma were modified with various chemicals in order to identify the functionally important amino amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentration in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipoprotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophodic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indication an importance of those amino acid residues in the function of the enzyme. 돼지혈장으로부터 분리한 lecithin : cholesterol acyltransferase의 아미노산 잔기들을 여러 가지 화학물질로 변환시킴으로서 이 효소의 촉매기능에 중요한 역할을 담당하는 아미노산 잔기들을 규명하려 하였다. 최소한 한 개의 thiol기가 이 효소의 촉매반응에 필요하며 높은 효소농도에서는 네 개 모두의 thiol기가 분자간 disulfide 결합을 형성함으로서 효소분자간의 결합에 관여할 것으로 추정된다. 전체 tryptophan 잔기의 절반 이상이 효소분자의 표면에 노출되어 있으며 이러한 소수성 아미노산 잔기들이 이 효소의 기질인 high-density lipoprotein 표면의 apolipoprotein-AI과 소수성 결합에 관여함으로서 효소-기질 결합에 참여할 것으로 추정된다. 이 효소의 역가는 serine, thiol 그리고 histidine 잔기를 변환하는 화학물질에 의해 민감하게 저해됨으로서 이러한 아미노산 잔기들이 이 효소의 촉매반응에 관여함을 제시해주고 있다.

Effects of Human Breast Milk and Hyperlipidemic Serum on the Cholesterol Metabolism in Organ Culture of Fetal Aorta

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

출생 때에 토끼는 성숙한 토끼보다 비교적 높은 혈중 콜레스테롤을 포함하고 있으며 그 농도는 수유에 따라 증가하여 수유 2주일에 최대치를 보이다가 수유기간이 끝나는 4주째에는 정상농도로 돌아가게 된다. 사람의 모유 또는 와타나베토끼(유전적으로 혈중 콜레스테롤이 비정상적으로 높은 토끼)의 혈청으로부터 분리한 저밀도 리포단백질을 배지에 포함시켜 출생전 토끼의 대동맥을 조직 배양할 경우 대동액내에 포함되어 있는 콜레스테롤 양의 변화를 관찰하였다. 사람의 모유를 함유한 배지에 먼저 배양한 후 와타나베토끼의 저밀도 리포단백질을 함유한 배지로 옮겨 배양했을 때 출생전 토끼 대동맥내 콜레스테롤 농도는 사람의 모유에서만 같은 기간동안 배양한 대동맥에서와 비슷했다. 그러나 사람의 모유에서 먼저 배양하지 않고 와타나베토끼의 저밀도 리포단백질을 함유한 배지에서만 배양한 대동맥에서는 훨씬 높은 콜레스테롤 농도를 보여주었다. 이 결과로 미루어 보아 사람의 모유가 대동액내의 콜레스테롤 축척을 막아주는 역할을 한다는 사실을 추측할 수 있다. Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

야투 자연미술이 가지는 미술교육적 함의

최명숙(Choi, Myung Sook) 한국미술교육학회 2021 美術敎育論叢 Vol.35 No.3

본 연구의 목적은 야투 자연미술이 가지는 미술교육적 함의를 찾는 것이다. 이를 위해 문헌 연구가 중심이 되었는데, 연구자는 야투의 자연미술 정신과 방법을 통해 미술교육에 주는 의미를 깨닫게 되었다. 첫째, 자연미술 정신은 미술교육에서 자연과 인간 이해의 확장을 이루게 한다. 미술교육에서 자연미술 방법으로 인간의 상실된 육신, 마음(정신), 영혼의 회복을 기대할 수 있다. 또한 학생들이 인간을 순수하게 본질의 눈으로 바라보도록 해준다. 이는 야투의 미적 태도로서 학생들이 자연에 몰입하여 미적 체험하며 자연의 본성을 깨닫기 때문이다. 그리하여 자연미술은 인간과 자연의 조화로운 공존을 통해 지속 가능한 미술교육의 새로운 방향을 제안한다. 둘째, 야투 자연미술은 미술과 교육과정에서 의미 있고 다양하게 수용될 수 있다. 지역, 위치, 장소성에 기반하여 학생들 삶의 터전에서만 가능한 자연미술 교육을 할 수 있으며 미술과 교육과정을 재구성할 수 있다. 그리고 미술과 교육과정에서 자연을 도구로만 삼지 않고 미술 놀이의 대상으로 보게 한다. 이때 자연미술 정신은 학생들에게 나도 미술을 잘할 수 있다는 창조적 활동으로 연결되어 자유를 획득할 수 있다. 그리하여 미술교육은 야투 자연미술을 통해 학생들에게 새로운 차원의 미적 경험을 제공하며 창의적인 미술 활동과 자연의 가치성을 깨닫도록 한다. The purpose of this study is to reveal the implications of Yatoo nature art in regards to art education. The literature research has been the main method of research in this study. The researcher realized the meaning of art education which is inspired by Yatoo nature art’s spirit and methods through literature research. The spirit of nature art could expand the understanding of nature and human beings in the area of art education. In art education, the methods of nature art enable the lost body, mind (spirit), and soul to be recovered. Also, it allows students to see humans through the eyes of pure essence. This is possible because students immerse themselves in nature, experience it s aesthetics, and realize the essence of nature in terms of Yatoo’s aesthetic attitude. Thus, nature art suggests a new direction for sustainable art education through the harmonious coexistence of humans and nature. Yatoo nature art could be accepted in meaningful and diverse ways in art curriculum. It not only allows students to experience nature art education based on region, location, and placeness, but also allows art curriculum to be reconstructed. In art curriculum, we should see nature as an object of art play rather than as a means. The spirit of nature gives students the opportunity to obtain freedom by connecting to the creative mindsets that ‘I can be good at art’ to the students. Therefor, art education through Yatoo nature art provides students with a new level of aesthetic experience and it helps them to understand the value of creative art activities and nature.

중년기 여성의 폐경에 대한 교육요구도, 지식과 태도에 대한 연구

최명숙 ( Myung Sook Choi ),박정원 ( Jeoung Weon Park ) 한국여성건강간호학회 2008 여성건강간호학회지 Vol.14 No.4

여대생의 외모에 대한 사회문화적 가치 내재화, 체형만족도, 분노 및 식이태도에 관한 연구

최명숙 ( Myung Sook Choi ),천숙희 ( Suk Hee Cheon ) 한국여성건강간호학회 2007 여성건강간호학회지 Vol.13 No.4