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정현채(Hyun Chae Jung),김정목(Jung Mogg Kim),진영주(Yeoung Joo Jin),이경미(Kyung Mee Lee),김우호(Woo Ho Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 1997 대한소화기학회지 Vol.29 No.5
N/A Background/Aims: CagA or cytotoxin-positive H. pylori may be associated with gastroduodenal diseases. However, controversies about this association also exist. Moreover, there could be geographic differences in the prevalence of virulence factors such as cagA or cytotoxin. It is also not clear whether there are substantial differences among clinical isolates which differ in bearing cagA or cytotoxin in the ability to induce IL-8 gene expression and protein secretion from the gastric epithelial cells. We performed this study 1) to determine the prevalence rate of the genes of virulence factor such as cagA and cytotoxin in H. pylori, 2) to test the ability of cagA cytotoxin', cagA cytotoxin or cagA cytotoxin H. pylori strain to evoke the proinflammatory cytokine expression in cultured gastric epithelial cells respectively. Methods: 1) The prevalence of the cagA gene in H. pylori strains isolated from Koreans was determined by PCR analysis. 2) Cytotoxin assay was performed by the concentrated broth supematants and HeLa cells(ATCC CCL2). 3) Proinflammatory cytokine gene expression was observed by RT-PCR analysis using the RNA extracted from human gastric epithelial cells such as Hs746T(ATCC HTB 135) and AGS(ATCC CRL 1739) infected with H. pylori. Cytokine proteins were also measured by ELISA. Results: 1) 96.8% of H. pylori isolates from Korean adults possessed cagA gene. And 80.6% of H. pylori strains have expressed vacuolating cytotoxicity against HeLa cells within 24 hours. 2) All cagAcytotoxin', cagAcytotoxin or cagA cytotoxin H. pylori strains could evoke the expression of cytokines such as IL-la/p, IL-8, MCP-1 and GM-CSF in the gastric epithelial cells. In case of IL-8, number of molecules of the expressed cytokine transcripts was parallel to the amounts of protein secreted from the gastric epithelial cells infected with H. pylori. Conclusions: These results suggest that H. pylori itself could give rise to express the proinflammatory cytokines in gastric epithelial cells regardless of possessing the known viru1ence factors of H. pylori. (Korean J Gastroenterol 1997;29:579-590)
담관담석증 및 담관암 환자의 담즙에서의 Helicobacter의 검출에 관한 연구
김정원,신지현,임창영,이명인,이종화,송일한,김정택,노임환,양미라,진영주 대한소화기학회 1998 대한소화기학회지 Vol.30 No.5
Background/Aims: In Korea, the number of patients with hepatolithiasis and choledocho]ithiasis are much higher than those in western countries. Infection might play a major role in the pathogenesis of bile duct stones. Until now E.coli, Klebsiella sp.and Gram negative enteric organisms were considered as predominant organisms isolated from bile with bile duct stones. The prevalence of H. pylori infection in gastroduodenal disease is remarkable in Korea. Bile acid, at physiologic concentrations, is known to cause a growth inhibition in H. pylori in vitro. The in vivo inhibitory effect of the bile acids on K. pylori, however, is unclear. Therefore, we investigated if H. pylori might be associated with bile duct stones or cholangiocarcinoma. Methods: Fifty two bile samples of 47 cases of bile duct stones(18 of IHD stone, 22 of CBD stone, 7 of IHD and CBD stone) and 5 cases of cholangiocarcinoma proven by histology were evaluated. 32 bile samples were collected by endoscopic nasobiliary drainage tube and 12 by percutanous transhepatic bile duct drainage tube. These 44 bile samples were collected on 5 to 7 days after ERCP without endoscopic shincterotomy. 8 samples were obtained by needle aspiration of gallbladder during laparoscopic cholecystectomy. 26 kDa surface antigen of H. pylori was detected using polymerase chain reaction(PCR) amplification. Results: 7 of 52 cases(13,5%) were positive for H. pylori 26 kDa surface antigen. Of the 7 cases, 6 cases were bile duct stone(2 of IHD stone, 3 of IHD and CBD stone, 1 of CBD and GB stone) and 1 case was hilar cholan-giocarcinoma. Among 7 cases of 26 kDa surface antigen positive, 4 cases of H. pylori DNA was detected in the bile from PTBD(including one case of needle aspiration of GB), and 3 cases in the bile from ENBD tube. Conclusions: Therefore, we concluded that H.pylori could be detected in the biliary tree with hepatolithiasis, choledocholithiasis or cholangiocarcinoma in Korea. Further investigation will be required if H.pylori or its subtype could cause cholangitis.