해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 계통학적 다양성

조현희,심은정,박진숙,Cho, Hyun-Hee,Shim, Eun-Jung,Park, Jin-Sook 한국미생물학회 2010 미생물학회지 Vol.46 No.2

2009년 4월 제주도의 운진항에서 채집한 해양 해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 주요 군집구조를 16SrDNA-denaturing gradient gel electrophoresis (DGGE) 방법을 이용하여 비교 분석하였다. S. abata와 Cinachyrella sp.는 각각 7개와 8개의 DGGE 밴드를 나타내었으며 이들을 적출하여 염기서열을 분석한 결과, NCBI에 등록된 서열들과 92-100%의 유사도를 나타내었다. S. abata의 주요 공생세균은 Alphaproteobacteria와 Deltaproteobacteria에 속하였으며, Cinachyrella sp.의 경우 Alphaproteobacteria, Gammaproteobacteria와 Actinobacteria에 속하는 세균으로 구성되었다. 두 종의 해면에서 공통되는 공생세균 그룹은 Alphaproteobacteria였으며 이 세균 그룹은 두 종의 해면 모두에서 우점하였다. Deltaproteobacteria는 S. abata에서, Actinobacteria와 Gammaproteobacteria는 Cinachyrella sp.에서만 관찰되었다. 동일지역에서 채집한 서로 다른 두 종의 해면은 각각 다른 공생세균 군집구조를 나타내었다. The bacterial community structure of two marine sponges, Spirastrella abata and Cinachyrella sp. collected from Jeju Island, in April 2009, was analyzed by 16S rDNA-denaturing gradient gel electrophoresis (DGGE). DGGE banding patterns indicated 8 and 7 bands for Spirastrella abata and Cinachyrella sp., respectively. Comparative sequence analysis of variable DGGE bands revealed from 92% to 100% similarity to the known published sequences. The bacterial groups associated with Spirastrella abata were Alphaproteobacteria and Deltaproteobacteria. The bacterial community of Cinachyrella sp. consisted of Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria. Alphaproteobacteria was common and predominant in both the sponge species. Deltaproteobacteria was found only in Spirastrella abata while Actinobacteria and Gammaproteobacteria were found only in Cinachyrella sp. The results revealed that though the common bacterial group was found in both the sponges, the bacterial community profiles differed between the two sponge species obtained from the same geographical location.

16S rDNA-RFLP에 의한 Spirastrella abata와 Spirastrella panis 해면에 서식하는 배양가능한 공생세균 군집의 비교

조현희,박진숙,Cho, Hyun-Hee,Park, Jin-Sook 한국미생물학회 2009 미생물학회지 Vol.45 No.2

계통적으로 근연하며 지리적 분포가 유사한 두 종의 Spirastrella 속의 해양 해면, S. panis와 S. abata의 배양 가능한 공생세균 군집구조를 16S rDNA-RFLP 방법에 의해 분석하였다. 공생세균의 배양은 해면추출물 3%를 포함하는 MA 배지를 사용하였다. 증폭된 16S rDNA의 RFLP (restriction fragment length polymorphism) 분석을 위한 제한효소로 HaeIII와 MspI을 이용하였으며, 그 결과 24개의 RFLP type을 구별할 수 있었다. 각 패턴별로 1~5개의 분리균주를 선별하여 부분 염기서열 분석 결과, 알려진 세균 종과 98.4% 이상의 유사도를 나타내었으며 2종의 Spirastrella 해면으로부터 분리된 세균들은 모두 Alphaproteobacteria, Gammaproteobacteria, Firmicutes, Actinobacteria 4개의 강(class)에 포함되었다. Alphaproteobacteria는 S. abata에서 39.3%, S. panis에서 47.6%가 관찰되어 두 해면에서 우점하는 세균 군집이었다. Gammaproteobacteria의 경우 S. abata에서 38.5%로 관찰된 반면 S. panis에서 1.6%의 아주 적은 비율로 관찰되었다. 또한 Bacillus (phylum Firmicutes) 종은 S. abata에서 9.7%를 나타낸 반면, S. panis에서는 44.3%의 분포를 나타내었다. Planococcus maritimus (8.1%, phylum Firmicutes)와 Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria)는 S. abata에서만 관찰되어 이들은 S. abata에 특이적인 세균 종임을 알 수 있었다. 같은 장소에 서식하는 계통적으로 근연한 두 종의 해면에서 공생세균의 군집 구조는 차이가 큰 것으로 나타났다. A cultivation-based approach was employed to compare the culturable bacterial diversity associated with two phylogenetically closely related marine sponges, Spirastrella abata and Spirastrella panis, which have geologically overlapping distribution patterns. The bacteria associated with sponge were cultivated using MA medium supplemented with 3% sponge extracts. Community structures of the culturable bacteria of the two sponge species were analyzed with PCR-RFLP (restriction fragment length polymorphism) based on 16S rDNA sequences. The RFLP fingerprinting of 16S rDNA digested with HaeIII and MspI, revealed 24 independent RFLP types, in which 1-5 representative strains from each type were partially sequenced. The sequence analysis showed >98.4% similarity to known bacterial species in public databases. Overall, the microbial populations of two sponges investigated were found to be the members of the classes; Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria. The Alphaproteobacteria were predominant in the bacterial communities of the two sponges. Gammaproteobacteria represented 38.5% of bacterial community in S. abata. Whereas only 1.6% of this class was present in S. panis. Bacillus species were dominat in S. panis. Bacillus species were found to be 44.3% of bacterial species in S. panis, while they were only 9.7% in S. abata. It is interesting to note that Planococcus maritimus (8.1%, phylum Firmicutes) and Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria) were found only in S. abata. This result revealed that profiles of bacterial communities from the sponges with a close phylogenetic relationship were highly species-specific.

보비탕(補脾湯)이 methotrexate로 유발(誘發)된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

조현희,윤혜진,서정민,백정한,Cho, Hyun-Hee,Yun, Hye-Jin,Seo, Jung-Min,Baek, Jung-Han 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In order to investigate the effect of Bobitang on SD rats with deteriorated immunity caused by methotrexate. Methods : The test sample were dosed once a day for 14 days by gastric gavage at a dosage 1,000, 500 and $250mg/kg/10m{\ell}$ from 2 days after last MTX-dosing, and the changes on body weight and gains, spleen weight and total blood leukocyte numbers, total lymphocyte numbers, the percentage of B-cell, T-cell, CD3+CD4+ T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the blood and spleen were observed. Results : The changes on body weight gains, the spleen weight, the total blood leukocyte numbers, the total lymphocyte numbers in the blood and spleen, the ratio of T-cell in the blood and spleen, the ratio of CD3+CD4+ T-cell in the blood and spleen were increased significantly in BBT Extracts groups as compared with control group. The ratio of B-cell in the blood and spleen was not increased significantly in BBT Extracts groups as compared with control group. The percentage of CD3+CD8+ T-cell in the blood and spleen was decreased significantly in BBT Extracts groups as compared with control group. The ratio of CD4+/CD8+ T-cell in blood and spleen was increased significantly in BBT Extracts group as compared with control group. Conclusions : According to the above results, Bobi-Tang has an effect of increasing immune responses on SD rats with deteriorated immunity caused by methotrexate.

꼬리뼈하 천골고정술: 자궁탈출증 치료의 단기성적보고

조현희 ( Hyun Hee Cho ),김미란 ( Mee Ran Kim ),황성진 ( Sung Jin Hwang ),김민정 ( Min Jung Kim ),김은중 ( En Jung Kim ),이진우 ( Jin Woo Lee ),김장흡 ( Jang Heub Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.6

Objective: To investigate the short term results of infracoccygeal sacropexy for the management of uterine prolpase or stump prolapse. Methods: Thirty-four Women who underwent infracoccygeal sacropexy for the management of uterine prolapse or stump prolpase were enrolled this study. Intraoperative or postoperative complications and results of the operation were evaluated and the recurrence rate of pelvic organ prolapse was checked 6 months after operation. Results: Changes of hemoglobin after infracoccygeal sacropexy was 1.7±0.9, mean duration of bladder drainage was 3.1±1.0 (days). After 2.1 days of operation, foley catheter was removed. Mean inpatient day was 5.2±1.0, patient discharged 3.2 days after operation. Recurrence rate after 6 months was 2.9%. Preoperative mean stage of prolapse was 2.7±0.7 (stage) and it decreased to 0.4±0.6 (stage) 6 months after operation. Conclusions: Infracoccygeal sacropexy was effective method for the management of uterine/ stump prolapse.

다문화가정 밀집 지역 중학교 교육과정 개선 방안 탐색: IB 교육과정 적용의 가능성과 한계를 중심으로

조현희 ( Hyunhee Cho ),장인실 ( Insil Chang ) 한국교육과정학회 2020 교육과정연구 Vol.38 No.1

With a growing number of foreign residents, South Korea is recently experiencing the emergence of these people in certain areas where they collectively inhabit. In response to these population­geographic shifts, Gyeonggi Ministry of Education has designated a total of twelve schools as the Multicultural International Innovative Schools (MIISs) in order to advocate educational equity and diversity for students in these areas. Yet, there has been lack of efforts to closely examine a set of curricula and programs implemented in the MIISs, which make it difficult to determine their possibilities and limitations in education reform. In this regard, the present study sought to explore the possibilities and limitations of MIISs by analyzing the current curricula and programs implemented in one of the MIISs with focus on its possibility to successfully utilize the curricular principles and instructional strategies embedded in the International Baccalaureate (IB). Methodologically, this study was guided by a qualitative case study. The findings highlight that the MIIS shares its vision, curriculum goal and content with the IB but it would need to wrestle with the rigidity of the nation-level curriculum and evaluation system, the teachers' low expectation for culturally and ethnically marginalized students, and the assimilation-oriented school and classroom climates so that the IB-based principles and strategies are successfully adopted. Based on the findings, suggestions for further improvement of the MIISs were provided.

생리전증후군 환자에서 식이 습관과 조직미네랄의 특성

조현희 ( Hyun Hee Cho ),정재은 ( Jae Eun Jung ),이주영 ( Ju Yong Lee ),김미란 ( Mee Ran Kim ),유영옥 ( Young Ok Lew ),임용택 ( Yong Taik Lim ),김은중 ( Eun Jung Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.4

About 80% of premenopausal women experience premenstrual syndrome (PMS), but the definite etiology or effective treatment is still under the investigation. In this study, serum and tissue mineral analysis, which are essential for biosynthesis and secretory regulation of neurotransmitters, We evaluate what kind of changes in mineral levels is correlated to the presence of PMS. This study also include the approaches that entail changes to diet and lifestyle so that symptoms of premenstrual disorders can be alleviated. 61 women who were recruited on volunteer basis and completed Modified Moss Menstrual Distress Questionnaire (MMDQ) and devided into 2 groups, scored less than 10 points, as control group, and women scored higher than 11 points, as PMS group. They were asked to fill out questionnaires about their diet patterns and submitted to tissue mineral and serum analysis to evaluate the levels of Ca, Mg, Na, K, Cu, and Zn. In the control group, serum Mg and Zn were relatively high but within normal reference range, but showed high level of Mg and Zn in tissue. For Mini Dietary Assessment Index (MDAI), the score of control group was 33.8 and that of PMS group 19.7, showed statistical significance (P<0.05). The score of daily calcium-taking group was 21.6±3.7 whereas that of seldom calcium-taking group was 39.6±6.2 (P<0.05). The score of daily magnesium-taking group was 9.75±2.7 whereas that of seldom magnesium-taking group was 26.0±4.9 (P<0.05). In this study, we conclude the importance of dietary and lifestyle modifications could prevent and treat symptoms of PMS and minerals had significant roles of regulating PMS especially Ca, Mg and Zn. Further studies about roles of these minerals and possible therapeutic applications using minerals in PMS are needed.

최신임상강좌 : 호르몬 피임약의 사용: 내과적 질환이 있는 경우

조현희 ( Hyun Hee Cho ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.11

Combination hormonal contraception is one of the effective contraceptive methods, but it can have many adverse effects especially in women with coexisting medical diseases. This clinical review will help you check the safety of hormonal contraception in women who have one of the chronic diseases such as venous thromboembolism, hypertension, dyslipidemia or diabetes. So you can choose the safest method of contraception in women with chronic medical conditions, and you will have some information about the interaction of oral contraceptives with other medications which can alter the blood level of concentrative steroids when administered together.

자궁근종용해술 후 만족도에 영향을 주는 요인: 18개월 추적검사 결과

조현희 ( Hyun Hee Cho ),정재은 ( Jae Eun Chung ),황성진 ( Sung Jin Hwang ),정인철 ( In Cheul Jeung ),김수연 ( Sue Yeon Kim ),김미란 ( Mee Ran Kim ),김장흡 ( Jang Hub Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.11

Objective: To report 18 months follow up results of radiofrequency myolysis and analysis the factors which effect the success rate of myolysis. Methods: 153 patients who took radiofrequency myolysis between October 2004.~June 2006 in this hospital were enrolled this retrospective study. Except 14 patients which had incomplete data, total 139 patient`s charts were reviewed. Sonographic evaluations were checked after a week, 1 month, 3 months, 6 months, 12 months, and 18 months after myolysis. Questions about complications like vaginal bleeding, abdominal pain, feber and vaginal discharge were asked to patients in every visits. Volume of the myoma were checked via 3D ultrasonography. Results: Reduction rate of the volume of myoma after 18 months were 73% and reoperation rate were 4.3%. Improvement rates of patients symptom were 87% (menorrhagea 95%, dysmenorrheal 75%, pelvic pain 60%) The patients who had myomas sized over 100 ml before treatments showed statistically higher rate of reoperation, and decreased rate of satisfaction. Reoperation rate and reduction rate of myoma showed no difference through pathologic diagnosis. The patients who had initial symptoms showed higher rate of satisfaction after myolysis. Total satisfaction rate were 62%, and no serious complications like bowel injury, bladder injury, sepsis and peritonitis were not reported. Conclusion: Satisfaction rate after radiofrequency myolysis was increased in symptomatic leiomyoma especially smaller than 6.5 cm in diameter.

한국여성에서 조직 미네랄 농도에 따른 생리전증후군의 임상증상

조현희 ( Hyun Hee Cho ),정인철 ( In Chul Jung ),정재은 ( Jae En Jung ),최세경 ( Sae Kyung Choi ),김수연 ( Su Yeun Kim ),김미란 ( Mee Ran Kim ),임용택 ( Yong Taek Lim ),김은중 ( En Jung Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.1

Objective: Premenstrual syndrome (PMS) is a disease with specific psychologic and physical symptoms on luteal phase. Its incidence is variable in 20~80%, but its cause is not definitely proved. Because progesterone and estrogen affect the balance of the body mineral, the alteration of progestorone and estrogen in the patients with PMS may effect the imbalance of tissue mineral, that can induce the specific symptoms of PMS. This study examines the relationship between symptoms of PMS and mineral count by tissue mineral test. Methods: Women who volunteered for the tissue mineral test completed MMDQ questionnaire and checked blood test for Ca, Mg, Na, K, Cu, Zn. The tissue mineral test used the hair not treated within 3 weeks and not washed within 3 hours. The hair was send to TEI for the analysis. We used SPSS (14.0) for statistical analysis. Results: The MMDQ score of the normal Mg group is significantly higher than the high Mg group (22.5±17.8 vs. 13.9±11.1), and the behavioral disorder score of the normal Na group is significantly lower than the low Na group (2.2±1.7 vs. 3.4±2.2). The MMDQ score of the normal Cu group is significantly lower than abnormal group (15.7±11.9 vs. 24.9±16.9). Conclusions: Total score of MMDQ showed difference according to magnesium and copper concentrations in tissue, and scores of behavioral disorder was affected by sodium concentration of tissue. Additional study about cause and effect relationship is required.

최신임상강좌 : 간질성 방광염과 방광통증 증후군의 진단과 치료

조현희 ( Hyun Hee Cho ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.10

The symptoms of Interstitial cystitis and painful bladder syndrome (IC and PBS) usually provocated after gynecologic disease or treatment, but gynecologist could not notice these disease because there are no diagnostic findings in urine analysis and radiologic study. IC and PBS should be diagnosed with their clinical symptoms, and exclusion steps for differential diagnosis of other confusable diseases are essential. These diseases are characterized by periods of exacerbation followed by variable periods of remission. The therapy for IC and PBS begins with extensive patient education and the goal of the treatment is not cure, but the remission of the symptoms.