RAPD기법을 이용한 갈파래목 해조류의 유전 변이 분석

조용철,박지원,진형주,남보혜,손철현,홍용기,CHO Yong-Chul,PARK Ji Won,JIN Hyung-Joo,NAM Bo-Hye,SOHN Chul Hyun,HONG Yong-Ki 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3

해조류중 우리나라 전연안에 가장 흔히 분포하는 갈파래목 녹조류인 참홑파래와 구멍갈파래, 참갈파래, 잎파래, 실파래, 가시파래등을 대상으로 RAPD방법을 이용한 각 지역 개체간의 유전적 유사도를 조사하였다. Total DNA는 엽체로부터 LiCl를 사용한 DNA추출방법으로 분리하였으며, 추출된 DNA는 $3ng/{\mu}\ell$되게 희석한후 in vitro에서 arbitrary primer와 함께 45회 PCR amplification시켰다. 그 결과 34종류 primer들로부터 240bp에서 1.5kb의 다양한 크기로 증폭된 1227개의 전기영동상의 band를 볼 수 있었으며, Jaccard공식에 따라 그 유사도를 구하였다. 파래목의 유전적 유사도는 $7\%$에서 $36\%$범위의 유사도를 보였다. 그중 참흩파래가 다른 갈파래과들로부터 가장 낮은 유사도값을 보여주었으며, 또한 primer OPB-01 (CATCCCCCTG)을 사용하였을때 갈파래과에서 공통적인 630bp크기의 생성물을 생산하지않는 특징을 나타내었다. The random amplified polymorphic DNAs (RAPD) technique was used to characterize seven isolates of the green seaweed Ulvales collected from Songjeng, Haeundae, Jumunjin, Dadaepo and Wando in Korea. Total DNA was extracted by the LiCl extraction method from thalli of green seaweed. The extracted DNA (3 ng) in $25{\mu}\ell$ reaction volume was amplified by 45 cycles of the polymerase chain reaction with arbitrary primers. Thirty-four primers resulted in 1227 PCR products ranged 240 bp to 1.5 kb of both conserved and polymorphic bands. Genetic similarities of the seven isolates calculated by Jaccard's equation were ranged from $7\%\;to\;36\%$. Monostroma nitidum (Wando) was shown to be most distantly related with the others based on genetic similarity and did not produce the amplified band of 630 bp, common in Ulvales using primer OPB-01 (CATCCCCTG).

해산 녹조류 참홑파래, Monostroma nitidum의 원형질체 분리와 분화

조용철,공용근,윤장택,선상미,정규화,CHO Yong Chul,GONG Yong Gun,YOON Jang-Taek,SUN Sang-Mi,CHUNG Gyu-Hwa 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1

해산 녹조류 참흩파래, Monostroma nitidum의 엽체를 효소처리하여 다량의 원형질체를 분리하였다. 최적 효소액의 조합은 $4\%$ R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power로서 생체조직 300mg 당 $4.41\times10^6$개의 원형질체가 분리되었다. 원형질체의 수율은 효소처리 270분에 최대였다. 분리 직후의 원형질체는 구형으로 직경 $13\~33\mu$m의 크기였다. 분리된 원형질체는 0.4M mannitol을 함유한 f/2배지에서 배양한 후 매주 mannitol이 함유되지 않은 f/2 배지로 절반씩 교환함으로서 분화율을 높일 수 있었다. f/2배지를 사용한 적정 배양조건에서 원형질체는 배양 3일 후 새로운 세포벽을 형성하였으며 10일 후 발아하기 시작하여 엽체로 발달하였다. 항생물질의 배지내 첨가는 배양체의 분화를 저해하였다. High yields of protoplasts were obtained following enzymatic digestion of the vagetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing $4\%$ Cellulase R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power produced $4.41\times10^6$ protoplasts per 300 mg of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between $13\~33\mu$m in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.

유도지도자의 지도행동유형이 집단응집력 및 성원만족에 미치는 영향

조용철(Cho, Yong-Chul),최숙이(Choi, Sook-Ie) 용인대학교 무도연구소 2007 武道硏究所誌 Vol.18 No.1

The purpose of this study was to make a quantitative investigation into the impact of the leadership behavior style of Judo instructors on group cohesion and the satisfaction of group members in a bid to help determine what type of leadership style and profile Judo instructors should have. The subjects in this study were 278 players who were selected from a population that consisted of Judo players in Seoul and Gyeonggi province who joined Korea Judo Association. After a survey was conducted, the players who gave completed answer sheets were selected. The collected data were analyzed with SPSS PC+ Ver. 10.0 program in a way to serve the purpose of the study, and the findings of the study were as follows: First, there was a positive correlation between the leadership behavior style of Judo instructors and group cohesion. Regarding the impact of leadership behavior style on group cohesion, leadership behavior style exerted a positive influence at the 1% level of significance on social cohesion at a personal(training, social support and reward) and collective(authority, training, social support and reward) level, and on task cohesion at a personal(democracy, social support and reward) and collective(democracy and social support) level. Second, in terms of correlational relationship between the leadership behavior style of Judo instructors and the satisfaction of the group members, there was correlation among leadership behavior style, task reception and social interaction. The leadership behavior style had a positive impact at the 1% level of significance on their satisfaction with task reception (training and authority), social interaction(training and reward) and coach behaviors (training, democracy and reward). Third, as for the correlation of the extent of the workout of the Judo players to group cohesion and satisfaction, there was a positive correlation among their extent of exercise, satisfaction and coach behaviors. In regard to the influence of the extent of exercise on group cohesion, social cohesion at a personal level was under the influence of exercise hours and career at the 1% level of significance, and workout hours and career had a positive impact at the 1% level of significance on personal and group cohesion. Concerning the impact of the extent of workout on satisfaction, satisfaction with task reception was positively affected by workout hours at the 1% level of significance, and satisfaction with social interaction was positively influenced at the 1% level of significance by workout career and hours. Their satisfaction with coach behaviors was under the positive influence of workout hours at the 1% level of significance.

하천수질을 위한 SRT시스템을 이용한 비점오염물질 저감기술

조용철 ( Yong Chul Cho ),최이송 ( I Song Choi ),오종민 ( Jong Min Oh ) 한국물환경학회 2008 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2008 No.-

이종격투기 미디어 시청에 따른 공격성 및 스포츠가치관에 관한 연구

조용철 ( Yong Chul Cho ),공성배 ( Seong Bae Kong ) 한국스포츠리서치 2007 한국 스포츠 리서치 Vol.18 No.4

The purpose of this study is to determine the relationship with aggressiveness and the value of sports of adolescence coming from mass media, Mixed Martial Arts Matches. The subject of the study was based on the population of Young In and Seong Nam cities and in the Gyeonggi Province, who are middle school students using Systematic Stratified Cluster Random Sampling. The participants from them in the study consisted of 336 students from questionnaires. A method of measurement was based on questionnaires, and aggressiveness was questionnaires, which was developed by Buss and Perry in 1992. Amended and supplementary questionnaires, which was developed by Evarett in 1962 were used for the value of sports. The survey used a five-points Likert scale, and then the completed questionnaires were analyzed by conducting one-way ANOVA Analysis and Multiple Regression Analysis in SPSS 12.0 program in accordance with the purpose of data analysis. The findings of the study were as follows; First, teenagers` aggressiveness from TV sports program, Mixed Martial Arts Matches was a partially considerable difference. Second, adolescence`s the value of sports is a partially considerable difference. Third, the aggressiveness have an effect on the value of sports in part.

직장암 환자의 수술 전 병기 결정에서 경직장 초음파의 유용성

조용철(Yong Chul Cho),김호군(Ho Goon Kim),허정욱(Jung Wook Huh),주재균(Jae Kyun Ju),김형록(Hyeong Rok Kim) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.75 No.3

Purpose: The preoperative assessments of the depth of invasion in the rectal wall and the presence of lymph node metastasis are very important in determining the proper treatment modality for rectal cancer. The purpose of this study is to evaluate the accuracy of transrectal ultrasonography (TRUS) for preoperatively staging rectal cancer, as compared with computerized tomography (CT). Methods: 62 patients who were diagnosed with rectal cancer were staged by using TRUS and CT, preoperatively. The ultrasnonographic tumor stage (uT), the US nodal stage (uN) and the computerized tomographic tumor stage (cT) and the CT nodal (cN) stage were investigated. The accuracy, sensitivity, specificity, PPV (Positive predictive value) and NPV (Negative predictive value) were calculated and compared with the pathologic staging. Results: The accuracies of TRUS and CT in assessing the depth of rectal wall invasion were 82.2% and 79.0%, respectively. The sensitivity, specificity, PPV and NPV of TRUS were 68.1%, 81.9%, 70.4% and 85.4% and those of CT were 53.2%, 78.9%, 73.7% and 80.7%, respectively. The sensitivity of T1 was 77.8% with using TRUS and 33.3% with using CT, respectively. The incidence of over- and under-staging was 17.8% and 9.7% with using TRUS and 25.8% and 6.5% with using CT, respectively. The accuracies of TRUS and CT in assessing the involvement of lymph nodes were 62.4% and 68.8%, respectively. The incidence of over-staging for TRUS and CT was 41.9% and 21.0%, respectively. The incidence of under-staging for TRUS and CT was 20.1% and 25.8%, respectively. There was no meaningful factor influencing the accuracy of TRUS. Conclusion: TRUS is very useful tool for the preoperative assessment of the depth of rectal cancer invasion. However, the evaluation of lymph node involvement by TRUS has limitations.

ICT 환경에서 오피스텔개발사업 의사결정요인에 관한 연구

조용철(Yong Chul Cho) 한국전자통신학회 2014 한국전자통신학회 학회지 Vol.7 No.1

본 연구의 목적은 ICT 환경에서 오피스텔 개발사업의 의사결정에 영향을 미치는 주요 요인들을 도출하기 위한 것이다. 오피스텔 개발 사업에는 각 요인의 계층별로 다양한 의사결정 변수가 존재한다. 이러한 변수 요인들을 계층의 특성 요인별로 집단화하여 의사결정 요인간의 상관관계를 분석해 이에 대한 상대적 중요도와 복합가중치에 의한 요인별 우선순위를 도출해 공급자 입장에서 각 전문 직군별로 오피스텔 개발사업 시 합리적인 의사결정 기준을 제시하는 데 있다. 복합가중치 분석결과 현금흐름의 적절성이 가장 높게 나타났으며, 전체 순공사비의 적정성, 입지지역 및 미래가치의 적합성, 분양가와 임대가격 및 분양조건의 적정성, 대중교통 빛 보행자의 접근성 등이 중요한 요인으로 분석되었다.

무도수련이 청소년의 학교생활적응 및 사회성발달에 미치는 영향

조용철(Cho, Yong-Chul),김미정(Kim, Mi-Jeong) 용인대학교 무도연구소 2007 武道硏究所誌 Vol.18 No.1

The purpose of this study was to examine how martial-art training affected the school adjustment and sociability of teenagers in an effort to prove the effectiveness of martial-art training as part of ideal education. The subjects in this study were high school boys and girls in Gyeonggi province. After a survey was conducted, the answer sheets from 411 students were selected and analyzed by SPSS Ver. 12.0 program in a way to serve the purpose of the study. The findings of the study were as follows: First, concerning the school adjustment of the students by demographic characteristics, the boys and girls were different from each other according to their gender in relationship with teachers, learning attitude, rule abiding and school-event participation. Their gender made a significant difference to their rule abiding(P<.05), and their relationship with teacher and school-event participation varied with their gender as well. Martial-art training made a difference to their relations with peers and teachers, and they were significantly different from one another in rule abiding and school-event participation according to the type of martial art they learned(P<.01). Second, in terms of the development of sociability by demographic characteristics, their stability and autonomy differed with their gender, and their age made a difference to their stability, dominance and sociality. Their activity level, stability and dominance varied with martial-art training experience, and the type of martial art they learned made a difference to their activity level. All the differences among them were significant(P<.01). Third, as for correlation between the subvariables of martial-art training and those of school adjustment, there was a positive correlation between them. Concerning the influence of martial-art training and school adjustment, their relationship with teachers was affected by two factors that included training term and intensity. One factor, training frequency, had an impact on their peer relations, and their rule abiding was influenced by a factor, which was training term. Their school-event participation was under the influence of an element that was training term. All the impact of martial-art training was significant at the 5% level of significance. Fourth, regarding correlation between the subvariables of martial-art training and those of sociability development, a very strong positive correlation existed between them. As to the influence of martial-art training on sociability development, their activity level was affected by one factor, training frequency, and their stability was influenced by two factors that involved training intensity and frequency. A factor, training frequency, exerted an influence on their dominance, and their sociability was impacted by a factor, training intensity. A factor, training intensity, had an impact on their autonomy, and all the influence of martial-art training was significant at the 5% level of significance.

용인대학교 60년사에 따른 무도체육박물관 기대효과

조용철(Cho, Yong-Chul),김도준(Kim, Do-Jun),송일훈(Song Il-Hun) 용인대학교 무도연구소 2013 武道硏究所誌 Vol.24 No.1

본 대학의 「용인대학교 60년사에 따른 무도체육박물관 전시실 보완 및 60년 역사연구」작업은 각 학과의 경기 종목 영역별에 내재된 의의와 가치를 도출하여 그 역사성과 정체성을 정립하고자 한다. 이와 같은 주체의식을 정립하기 위해 다음과 같은 기대효과를 도출할 수 있는데 이는 다음과 같다. 첫째, 학문적, 사회적 기여도이다. 이 전시실 보완 및 역사자료집 연구 작업을 통하여 각 학과의 경기 종목 영역별에 관한 「역사 정통성과 전통성 이념적 체제」를 학문적으로 재정립하는 연구가 진행되어야 한다. 둘째, 학문적 활용방안이다. 이 전시실 보완 및 역사자료집 연구 작업을 통하여 각 학과의 경기 종목 영역별에 관한 「철학 사상적 이념의 사유적 사고」를 학문적으로 재정립 하여야 한다. 셋째, 학문적 파급효과이다. 이 전시실 보완 및 역사자료집 연구 작업을 통하여 각 학과의 경기 종목 영역별에 관한 「무도체육학적 근본 개념 체계적 원리」를 밝혀, 이에 따른 의의와 가치를 명확히 제시할 수 있을 것이다. 이로 인해 교육 프로그램의 소중한 일환으로서 본 대학의 학문적 문화유산을 보습할 수 있을 것이다. The University`s “Yongin University 60 years history and the Martial Arts and sports museum exhibition 60 years history of complementary studies” majors in the work of each department to derive the intrinsic meaning and value that you want to establish a history and identity. In order to establish subject matter, such as the following potential benefits can be derived. First, the academic and social contribution is Historical data and complements the exhibition of work by each department majors through the “historical authenticity and traditional ideology system” to re-establish the academic research must be carried out. Second, take advantage of the academic approach. The historical data showrooms complements majors in each department through work “philosophy and ideological grounds thinking of ideas” should be reestablished and academically. Third, the impact of an academic. The historical data and showrooms complements majors in each department through job “systematic martial arts, physical education concepts and principles underlying biological” significance and value accordingly revealed will be able to clearly present. As a result of this training program as part of the University`s academic precious cultural heritage will be able to spread. <br/>

오피스텔개발사업 의사결정요인에 관한 연구

조용철(Yong Chul Cho),민규식(Guy Sik Min) 한국부동산학회 2014 不動産學報 Vol.59 No.-

본 연구의 목적은 오피스텔 개발사업의 공급주체로서 참여하는 각 전문가그룹별로 의사결정에 영향을 미치는 주요 요인들을 도출하여 합리적 의사결정기준을 제시하기 위한 것이다. 분석결과 전체 평가항목에 대한 최상위계층의 중요도는 사업성 및 재원조달이 가장 높게 나타났으며, 입지지역 및 시장여건, 개발 및 분양계획의 순으로 나타났다. 전체 평가항목에 대한 복합가중치 분석결과 현금흐름의 적절성, 전체 순공사비의 적정성, 입지지역 및 미래가치의 적합성 등이 중요하게 제시되었다. 직군별 복합가중치 분석결과 시공사는 투자수익률의 적절성, 입지지역 및 미래가치의 적합성이 높게 나타났고, 분양대행사는 분양가, 임대가격 및 분양조건의 적정성, 입지지역 및 미래가치의 적합성이 높게 나타났으며, 금융기관은 현금흐름의 적절성, 전체 순공사비의 적정성이 높게 분석되었다. 본 연구는 오피스텔 개발사업 의사결정을 위한 변수들을 각 계층의 특성 요인별로 집단화하여 의사결정 요인간의 상관관계를 분석해 이에 대한 상대적 중요도와 복합가중치에 의한 우선순위를 도출해 공급자 입장에서 의사결정기준을 제시하였다. 1. CONTENTS (1) RESEARCH OBJECTIVES The purpose of this research is to generate major factors to influence the decision making process of officetel development project. The importance and the prioritized order of each factor generated by the complex weighting have been deduced through the comparative analysis of correlation between the decision making process factors by collectivizing the characteristics factors of hierarchy with these variable factors. (2) RESEARCH METHOD The research has been conducted on the document & data. Also, the AHP analysis has been conducted with an in-depth survey for experts in order to generate the relative importance of decision making factor. (3) RESEARCH FINDINGS As a result of complex weighting analysis, the adequacy of cash flow has been shown as the most important factor, and followed by the propriety of total construction cost, the suitability of location & future value, the propriety of sale price/condition & lease price as important factors. 2. RESULTS Based on the analysis of result significance, the importance of finance & business feasibility have been shown relatively to be the highest. The propriety of return of investment ratio has been shown as the highest item in case of the constructor, and sale price/condition & lease price in case of the sales agency, and the adequacy of cash flow in case of the financial institution.