Localization of Cyclooxygenase Isozymes in Skin Wound Healing in Mouse

고준혁(Jun Hyuk Koh),조백현(Bek Hyun Cho),허탁(Tag Heo),배미옥(Mi Ok Bae),이송은(Song Eun Lee),안규윤(Kyu Youn Ahn),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park),남광일(Kwang Il Nam) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.2

Cyclooxygenase (COX)-1 and -2 expressions in the incisional wound healing of mouse skin were determined by immunohistochemistry and Western blot analysis. By Western blotting, compared to normal skin, COX-2 activity was increased at days 1, 4, 8, and 12 and was maximal at 4 day after incisional wound of mouse skin whereas COX-1 was barely detectable. In normal skin, COX-1 immunostaining was observed among the basal cells of epidermis whereas COX-2 immunostaining was detected in the more differentiated, suprabasal keratinocytes. At 1~4 days after wound, COX-2 staining was particularly prominent in the inflammatory cells, and at day 8, many macrophage-like cells were stained positively. COX-2 immunoreactive fibroblast, macrophage-like cells, and newly formed vascular endothelial cells were increased in number at 12 days after incision. These data suggest that COX-2 is constitutively expressed, just as is COX-1, in epidermis and is associated with keratinocyte differentiation. In addition, these findings support the well-established role for COX-2, the prostaglandins that they generate, as mediators of inflammatory response. 생쥐 피부상처 치유과정에서 cyclooxygenase(COX)-1과 COX-2의 분포 및 역할을 Western blot 분석과 면역조직화학 염 색을 통하여 알아보고자 하였다. Western blotting 실험에서 COX-2 단백질은 정상 피부에 비해 손상 후 1일부터 증가하여 4일째 최고치를 나타내며 12일 까지 증가한 반면에, COX-1은 검출이 되지 않을 정도의 적은 단백량을 나타내었다. 면역조직화학 염색에서 COX-1은 정상 및 손상 피부의 표피 바닥층과 털주머니의 윗부위에서 드물게 양성 반응을 나 타내었다. COX-2는 정상 및 손상군 표피에서 바닥층 위의 각질세포와 털주머니의 윗부위 및 진피유두에서 양성이었다. 손상 후 1일과 4일째 진피의 염증세포에서 COX-2에 강한 양성 반응을 나타냈고, 8일째는 큰포식세포에서, 12일째는 큰포 식세포, 섬유모세포와 신생혈관 내피세포에서 양성 반응을 보였다. 이상의 결과로서, COX-2가 COX-1과 마찬가지로 표피에 상재하는 구성요소로서 각질세포의 분화를 조절할 것임을 암 시하였고, 진피에서는 염증과 같은 자극에 대한 반응을 매개함을 확인할 수 있었다.

백서피부의 화상 손상에 따른 효소조직화학적 변화

조백현,김영길,허우회,최석현 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.2

The purpose of this study was to investigate the sequential changes of enzyme activities in severely burned skin of rat at intervals of 2 hours, 8 hours, 1 day and 3 days by histochemical methods. The results obtained were as follows. 1. Acid phosphatase activity was found to be transient increase in dermis and mild decrease in epidermis, hair follicle and sebaceous gland at 2 hour after burning. The activity was not seen in skin after 8 hours. 2. Alkaline phosphatase was found to be decreased in blood vessel and dermal papillae of hair follicle at 2 hour after burning and then was progressively decreased after 8 hours. 3.α-naphtyl acetate esterase activity was found to be decreased in epidermis, dermis and hair follicle at 2 hours after burning and was not seen in skin after 8 hours. There was no significant changes in sebaceous gland after burning.

안면부 피부암의 임상적 고찰

조백현,이삼용,최광림,김대영,이장혁 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.4

This retrospective clinical study includes 98 cases of facial skin cancers, which were managed in Chonnam University Hospital from 1986 to 1995 with histopathologic confirmation. 1. Of all cases, basal cell carcinoma was the most common type(74.5%), and squamous cell carcinoma(20.4%), malignant melanoma(2.0%), eccrine carcinoma(1.0%), rhabdomyosarcoma(1.0%), metastatic leiomyosarcoma(1.0%) were followed. The ratio of basal cell carcinoma to squamous cell carcinoma was 3.7:1. 2. In age and sex distribution, majority of facial skin cancers developed after 6th decade(83.7%). In basal cell carcinoma, female was more frequently affected than male and the ratio was 1:1.7. 3. The predilection site of basal cell carcinoman were lower eyelid, nose, cheek, and those of squamous cell carcinoma were lower lip, cheek and lower eyelid. 4. Of the reconstructive methods after tumor resection, local flaps were most commonly used(60.9%), and primary closure was possible only in 18.4% of all cases. 5. The duration between the presumed time of onset and the time of operation was between 6 months and two years in 76.0% of all cases, with an average of 3.2 years.

Superiorly Based Dermal Pedicle Technique을 이용한 거대유방증과 유방하수의 교정례

조백현,김광석,박진수,김병묵 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.5

The authors report five patients with gigantomastia and breast ptosis who underwent breast reduction and mastopexy using the superiorly based dermal pedicle, that is the Cramer & Chong's method. This techeque is based on the concept of transposing the nipple-areola complex on a vascular supply limited to the cutaneous vasculature, as pioneered by Strombeck. The cases involved 37 to 45-year-old women who had suffered from gigantomastia or breast ptosis. The authors chose the procedure using a superiorly based dermal flap, reported by Cramer & Chong in 1976. Total weights of breast reductions were 188g to 1422g. The migrations of nipples were 5.5cm to 12cm upward. After the following up for three months to two years, postoperative appearances were acceptable with fairly good breast cone and nipple-areola sensations were preserved. There are various techniques of reduction mammaplasty. For macromastia with marked ptosis like this case, breast amputation and free nipple graft may also be used. But the reason why the authors close this method is that a long and thin pedicle may be safely used for nipple transposition and sensation of the nipple may be preserved. On the ocher hand, the loss of lactation may be one of the disadvantages of this procedure. Although it is difficult to take care not to injury the dermal plexus of the pedicle in denuding, this method may also be useful for the treatment of gigantomastia breast ptosis.

생쥐 피부창상의 치유과정에 미치는 Glucan의 효과

조백현,허우회,최석현 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.2

In an attempt to investigate the effect of glucan, i.e., RES stimulating agent, on the repair process of skin wound, we made simple skin wound in mice and then glucan was given intraperitoneally at dose of 10mg until taking specimen. The specimen were taken from wound area at various intervals and histological and enzym histochemical stains were done for the light microscopic examination. The results obtained were as follows. 1. The glucan-treated wound showed more proliferation of macrophages and granulation tissue with decreased acute inflammatory exudates in the early than did control wound. 2. The glucan-treated wound showed more increase of acid and alkaline phosphatase and α-naphthyl acetate esterase activities than did control wound, but no changes in ATPase activities. From these results, it may be concluded that glucan promotes repair process of skin wound.

흉배혈관의 근피 천공분지에 기초한 유리피판의 임상적 이용

조규성,김대영,안호범,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The latissimus dorsi muscle or musculocutaneous flap is one of the most useful flaps in reconstructive surgery. This flap has many advantages, such as its reliable anatomy, long pedicle with large caliber vessels, minimal functional deficit of the donor site, and low incidence of donor site complications. However, the bulkiness of the flap has been considered a disadvantage, so various modifications of technique have been devised. The cutaneous portion of the flap can be safely elevated based on the cutaneous perforating branch of the thoracodorsal vessel. From March 1997 to February 1998, 10 patients underwent reconstructive procedures with thoracodorsal perforator-based free flaps. The composition of the flaps varied in accordance with the nature of the defect. The variances in the flaps were as follows; 3 were cutaneous, 6 musculocutaneous, and 1 osteomusculocutaneous including the rib. All flaps survived with good contour. We concluded that this thin and reliable flap was useful for reconstruction of various defects, and that the composition of the flap, such as subcutaneous, muscle and bone, gave it considerable flexibility as needed.

화상 환자 531예에 대한 임상적 고찰

조백현,김병삼,임기영,류봉수,이삼용 大韓成形外科學會 1992 Archives of Plastic Surgery Vol.19 No.2

During the 10 years from January, 1981 to December, 1990, 531 cases of burn patients were admitted into the Department of Plastic & Reconstructiove Surgery, Chonnam Medical School Hospital. The following results were obtained after the analysis of these burn patients. 1) Male were affected more frequently than female, the incidence being 197 cases(60%) and 212 cases (40%). The male to female ratio was 1.5:1 2) The hightest incidence was noted in winter, 180, cases(33.9%) and the least in summer, 108 cases (20.4%) The highest incidence was noted in March, 68 cases(12.8%) and the least in May, 22 cases (4.1%) 3) The most common cause of the burns was scalding : 226cases(42.5%) 4) In 81.6% of all cases, the burned surfaces covered less than 30% of the total body and in 34.1% of them, the burned surfaces covered less than 10% of total body surface area. 5) 357 cases(67.2%) required fluid rescucitation, and 239 cases(66.9%) were rescucitated by the parkland formula. 6) The topical antibacterial agents were as follows: Sliver sulfadiazine cream 192 cases(36.2%), Sofra-tulle 150 cases, Gentamycin ointment 75 cases(14.1%), Cambison ointment? 47 cases(8.9%) in order of frequency. 7) The cultures of wound surface revealed Pseudomonas aeruginosa(41.0%), Staphylococcus aureus(16.0%), E-coli(14.0%), Staphylcoccus albus (77.5%) etc. in order of frequency. 8) The surgery was perfomed in 291 cases and the most frequent method of surgery was split thickness skin graft. 9) The graft loss rates of topical antimicrobial agents after grafting were as follows: Saline gauze(23%), Cambison ointment (15%), Gentamicin ointment(11%), Sofra tulle(8%) 10) The Complications were as follows 58 cases(14.8%), gastrointestinal tract complications 55 cases(14.0%), conjunctivitis 21 cases (5.4%), genitourinary tract infections 18 cases(4.6%) etc. in order of frequency. 11) The mortality rate and cause of death were as follows: (1) The total mortality rate was 3.2%(17cases). (2) The most common cause of death was sepsis in 8 cases(47.0%) (3) The mortality rate of the early children and old age group was high. (4) The most common period of intervals between the time of the accident and death was within 3days.

희귀두개안면열의 외과적 교정예

조백현,조상윤,안호범,김대영,이삼용 대한미용성형외과학회 1998 Archives of Aesthetic Plastic Surgery Vol.4 No.1

治癒過程에 있는 火傷皮膚의 fibronectin 分布에 關한 免疫細胞學的 檢索

曺伯炫 全南大醫大 1984 전남의대학술지 Vol.21 No.1

안검에 발생한 기저 세포암의 치험례

조백현,임기영,곽승호,김병삼,이삼용 大韓成形外科學會 1992 Archives of Plastic Surgery Vol.19 No.2

Basal cell carcinoma is the most common skin cancer. Although it rarely metastasizes to a distant site and rarely invades the deeper structure it occurs often in the face especially, the eyelids and the nasolabial fold. If left uncontrolled for a long time, reconstruction is be difficult, and severe complications such as visual and definitive treatment is essential. Currently accepted treatment modalities are curettage and electrodesiccation, radiation therapy, surgery, cryotherapy and application of 5-FU, etc. Among them, we preferred to use the surgical treatment. We underwent 11 basal cell carcinomas developed on eyelids between May of 1988 and April of 1990 and gained satisfactory results by wide excision of the tumors and reconstruction of eyelids using various techniques: As for the lower eyelid, we used cheek rotation skin flap or bilobed skin flap with chondromucosa graft, or bipedicled and nasolabial flaps with chondromucosal and fascial graft. When the upper eyelid was involved, we used Fricke flap with oral mucosal graft. In case of both upper and lower eyelids, we used median forehead, Fricke and cheek rotation flaps with oral and chondromucosal graft.