[지역뉴스 부문_제146회 이달의 방송기자상] 꼬리에서 몸통으로, ‘무모한’ 5개월의 추적 : 〈"내 차 정보가 샌다" 수백만 건 유출 단독 추적〉 2020년 11월 16일~20일 보도 MBC충북 조미애, 이채연, 신석호 기자

조미애 (사)방송기자연합회 2021 방송기자 Vol.58 No.-

Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산

조미애,송윤미,박윤옥,고석민,민성란,유장렬,이준행,최필선,Cho Mi-Ae,Song Yun-Mi,Park Yun-Ok,Ko Suck-Min,Min Sung-Ran,Liu Jang-Ryol,Lee Jun-Haeng,Choi Pil-Son 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4

Agrobacterium과 자엽절 공동배양으로 대두 형질전환체를 생산하였다. 멜론 (슈피VIP품종)의 자엽절 절편은 선발 마커로서 bar와 reporter로서 gus유전자가 포함된 pPTN289 또는 선발마커로서 nptII유전자와 reporter로서 gus유전자로 제작된 pPTN290벡터를 LBA4401, GV3101, EHA101에 각각 형질전환하여 공동 배양하였다. 최대 형질전환빈도(0.16%)는 EHA101 (pPTN289)균주로 공동배양한 자엽절 절편을 glufosinate가 첨가된 선발배지에서 얻을 수 있었으며, 최종적으로 glufosinate저항성과 잎 ($T_0$), 화기 ($T_0$), 종자 ($T_1$) 및 유식물체 ($T_1$)에서 GUS양성반응을 나타내는 5개체를 얻었다. Southern분석에 의하여 GUS유전자가 멜론 genomic DNA에 도입되어 있음을 확인하였다. Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산

조미애,박윤옥,김진석,박기진,민황기,유장렬,최필선,Cho Mi-Ae,Park Yun-Ok,Kim Jin-Suck,Park Ki-Jin,Min Hwang-Ki,Liu Jang-Ryol,Clemente Tom,Choi Pil-Son 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

옥수수 미숙배배양과 Agrobacterium tumefaciens공동배양법에 의해 형질전환체를 생산하였다. Hi II계통의 미숙배를 Ubiquitin 1 promoter-GUS유전자와 선발마커로서 nptII 유전자로 제작된 pPTN290벡터를 C58C1에 도입한 후 형질전환 균주로 사용하였다. 7개의 paromomycin저항성 배 발생캘러스를 얻었으며, GUS양성반응을 나타내는 7개의 독립적인 식물체를 얻었다. Southern분석법에 의하여 $T_1$세대 식물체로부터 nptII유전자가 안정적으로 도입되어 있음을 확인하였다. Agrobacterium tumefaciens-mediated immature embryo transformation was used to produce transgenic maize. Immature embryo of Hi II genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics. Histochemical GUS assay showed that 7 individual lines transformed with the GUS gene were positive response among the transformants. Southern blot analysis revealed that the nptll gene segregated and expressed in their progeny.

직업가치관에 따른 피부미용인 재교육 관련 특성에 관한 연구

조미애,심은경 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.2

The purpose of this study is to figure out the related features of Esthetician’s re-education according to vocational values. To achieve this purpose, a survey was carried out on 350 beauticians in skin beauty job. Questionnaires of 303 copies were collected. The results of this study are summarized as follows. First, in questions for vocational value which asks of recognizing yourself as blue collar worker or professional value sales man. Many respondents answer that they are professional value sales man, those who are 30’s aged, college graduates, high work experienced, high position, high income and who have plan for future. People who response they are blue collar workers answer that all education is only for necessary practical training. In differences by demand re-education following by vocational value, 224 persons(73.9%) who response they are professional value sales man that they highly demand of re-education even they have a will to endure over-loaded work, pay for education fee, actively involve to reeducation, encourage re-education to colleges, and help to colleges who is in reeducation. In addition people who response they are professional value sales man highly satisfied. Therefore beautician is to be recognized professional value sales man by reeducation. Therefore, profession of Esthetician’s, simply is not a technician to sell beauty technology, and the opportunity of re-education know there is a need to transform the framework of recognition of occupational values as an expert in the value of the service would say. In other words, because it gives the needs original satisfaction significant impact of re-training on the basis of occupation values of Esthetician’s, technology that re-education is seems necessary, theory, etiquette, and programs such as customer service, management developed a program that places priority to the formation of values of the self-profession, it is thought that it is necessary to study. The purpose of this study is to figure out the related features of Esthetician’s re-education according to vocational values. To achieve this purpose, a survey was carried out on 350 beauticians in skin beauty job. Questionnaires of 303 copies were collected. The results of this study are summarized as follows. First, in questions for vocational value which asks of recognizing yourself as blue collar worker or professional value sales man. Many respondents answer that they are professional value sales man, those who are 30’s aged, college graduates, high work experienced, high position, high income and who have plan for future. People who response they are blue collar workers answer that all education is only for necessary practical training. In differences by demand re-education following by vocational value, 224 persons(73.9%) who response they are professional value The purpose of this study is to figure out the related features of Esthetician’s re-education according to vocational values. To achieve this purpose, a survey was carried out on 350 beauticians in skin beauty job. Questionnaires of 303 copies were collected. The results of this study are summarized as follows. First, in questions for vocational value which asks of recognizing yourself as blue collar worker or professional value sales man. Many respondents answer that they are professional value sales man, those who are 30’s aged, college graduates, high work experienced, high position, high income and who have plan for future. People who response they are blue collar workers answer that all education is only for necessary practical training. In differences by demand re-education following by vocational value, 224 persons(73.9%) who response they are professional value sales man that they highly demand of re-education even they have a will to endure over-loaded work, pay for education fee, actively involve to reeducation, encourage re-education to colleges, and help to colleges who is in reeducation. In addition people who response they are professional value sales man highly satisfied. Therefore beautician is to be recognized professional value sales man by reeducation. Therefore, profession of Esthetician’s, simply is not a technician to sell beauty technology, and the opportunity of re-education know there is a need to transform the framework of recognition of occupational values as an expert in the value of the service would say. In other words, because it gives the needs original satisfaction significant impact of re-training on the basis of occupation values of Esthetician’s, technology that re-education is seems necessary, theory, etiquette, and programs such as customer service, management developed a program that places priority to the formation of values of the self-profession, it is thought that it is necessary to study.

교실 밖 화학체험학습에 대한 교사들의 인식 -부산지역 중학교 과학교사를 대상으로-

조미애,최은경,윤진녀,문성배 한국과학교육학회 2005 한국과학교육학회지 Vol.25 No.7

국내 딸기 재배품종의 잎절편 배양으로부터 고빈도 식물체 재생

조미애,최규명,고석민,민성란,정화지,유장렬,최필선,Cho Mi-Ae,Choi Kyu-Myeong,Ko Suck-Min,Min Sung-Ran,Chung Hwa-Ji,Liu Jang-Ryol,Choi Pil-Son 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.3

To develop a high efficiency plant regeneration system from in vitro cultures of strawberry, cv. Yeobong, petiole and leaf explants were cultured on MS basal medium containing a combination of 0.5 mg/L IBA and 3.2 mg/L kinetin or zeatin or benzyl amino purine (BAP) for 6 weeks, and leaf explants with dark pretreatment for a week ($T_1$), 2 weeks ($T_2$), and 4 weeks ($T_3$) were cultured on medium supplemented with 0.5 mg/L IBA and 3.2 mg/L zeatin under 16 hr photoperiod for 6 weeks. Shoot organogenesis was observed from the greenish calli containing minimal anthocyanin formed at proximal cutting edges of the leaf explant (57%) when cultured adaxial side on the medium, whereas was directly formed from a cutting edges of petiole explant (6.3%). Frequency of callus formation and shoot organogenesis at large size of leaf explant ($1.0{\sim}1.5\;cm^2$) was higher than small size ($0.5{\sim}1.0\;cm^2$), and dark pretreatment significantly improved the frequency of leaf explants that produced calli and shoots. The maximum frequency (87%) for shoot organogenesis was obtained from the leaf explants that transferred to a 16 hr photoperiod condition after the initial 4 weeks dark period. The improved frequency (87%) in comparision with control without dark pretreatment (27%). When the shoots were transferred to 1/2 MS basal medium, formed roots with 20 d of culture. The rooted plants were subsequently transferred to the pots and to the field. 딸기 기내배양으로부터 고빈도 식물체재생 방법을 개발하기 위하여 여봉딸기의 잎과 엽병절편을 0.5 mg/L IBA와 3.2 mg/L kinetin, BAP, zeatin을 각각 조합 첨가한 MS배지에서, 그리고 1주, 2주, 4주동안 암 전처리한 잎 절편을 0.5 mg/L IBA와 3.2 mg/L zeatin이 첨가된 배지에서 6주동안 16시간 광주기 조건으로 배양하였다. 신초 발생은 잎을 adaxial상태로 치상하였을때 절단부위로부터 안토시아닌 색소를 함유한 녹색 캘러스로부터 유도되었고 (57.0%), 엽병의 경우 절단부위로부터 직접 발생되었다(6.3%). 캘러스와 신초발생율은 잎 절편 크기가 클수록 ($1.0{\sim}1.5\;cm^2$) 높았으며, 암 전처리는 그 발생율을 점차 증가시켜 4주동안 암 전처리한 후 16시간 광조건으로 옮겼을 때 가장 높은 발생율 (87.0%)을 얻을 수 있었다. 이러한 shoot발생율 (87%)은 암 전처리를 하지 않은 대조군 (27%) 에 비하여 현저하게 증가된 것이다. 신초를 분리하여 1/2 MS기본배지에 옮겨20동안 배양하였을 때 뿌리가 발생되었으며, 이때 소식물체를 토양에 옮겨 순화시켰다.

배추의 배축절편으로부터 캘러스와 뿌리 발생을 통한 안정적 형질전환

조미애,김춘해,민성란,고석민,유장렬,최필선,Cho, Mi-Ae,Kim, Choon-Ae,Min, Sung-Ran,Ko, Suck-Min,Liu, Jang-Ryol,Choi, Pil-Son 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.2

'정상' 배추의 배축절편을 선발마커로서 paromomycin 저항 성유전자를 갖고 있는 pPTN290으로 각각 형질전환된 EHA101, LBA4404, GV3101균주와 공동배양한 후 갤러스유도배지에서 형질전환캘러스를 얻은 후, 뿌리유도배지에서 부정근을 그리고 신초유도배지에서 신초를 각각 순차적으로 유도하였다. 형질전환캘러스를 얻은 후, 뿌리유도배지에서 부정근을 그리고 신초유도배지에서 신초를 가각 순차적으로 유도하였다. 형질전환캘러스 형성은 Agrobacterium균주에 따라 차이가 있었으며, 특히 EHA101균주에 공동배양된 배축절편으로부터 최대 6.1%까지 얻어졌다. 또한 각각의 형질전환캘러스 클론으로부터 형질전환 부정근과 신초 발생은 EHA101균주에서 60.7%와 38.2%, LBA4404에서 8.3%와 0%, GV3101에서 20.5%와 85.7%까지 각각 얻을 수 있었다. 형질전환식물체는 특별한 형태적 이상 없이 온실에서 정상적으로 자라 $T_{2}$종자를 얻을 수 있었다. GUS방법으로 7개의 후대 유식물체를 분석한 결과 gus유전자가 안정적으로 발현하고 있음을 확인하였고, 배추 genome에 single 또는 multiple copy로 전달되고 있음을 추측할 수 있었다. Hypocotyl explants of Chinese cabbage (cvs. "Jeong Sang") produced transgenic calli on callus induction medium (MS salt, B5 vitamin, 5 mg/L acetosyringone, 1 mg/L 2,4-D, 3% sucrose, 400 mg/L cefotaxime, 100 mg/L paromomycin, pH 5.8) after cocultivation with strains of Agrobacterium tumefaciens (EHA101, LBA4404, GV3101) harboring the pPTN290 containing paromomycin-resistance gene as a selectable marker, and then they transferred to root induction medium (1/2MS salt, MS vitamins, 2% sucrose, 100 mg/L paromomycin, 100 mg/L cefotaxime, pH 5.8) and shoot induction medium (MS salt, B5 vitamin, 4 mg/L $AgNO_3$, 4 mg/L 6-benzyladenine, 3 mg/L alpha-naphthaleneacetic acid, 100 mg/L paromomycin, 100 mg/L cefotaxime, 3% sucrose, pH 5.8) in order. There was a significant difference in the frequency of transgenic calli depending on Agrobacterium strains. In particular, the highest frequency (6.1%) of transgenic calli was obtained from the hypocotyls cocultivated with EHA101 strains. Also, the frequency (%) of transgenic root and plants from each transgenic callus clone were obtained with 60.7% and 38.2% in EHA101, with 8.3% and 0% in LBA4404, with 20.5% and 85.7% in GV3101 strains, respectively. They were grown to maturity in a greenhouse and normally produced $T_2$ seeds. GUS histochemical assay for progeny ($T_2$) revealed that the transgenes was expressed in the plant genome, and progeny analysis from 7 independent transgenic events demonstrated that the transformants transmitted the transgene as a single or multiple functional locus.

고려엉겅퀴(Cirsium setidens (Dunn) Nakai)의 구성성분 및생리활성에 관한 리뷰

조미애,김범정,MiAe Cho,Bumjung Kim 대한본초학회 2023 大韓本草學會誌 Vol.38 No.4

Objectives : The objective of this study was to investigate the phytochemistry and pharmacological activities of Cirsium setidens. Methods : Domestic and international articles about Cirsium setidens were investigated. A review was perfoemed via DB searching engine such as Sci.Direct, Springer, DBpia, KISS, Google scholar, Kipris, and so on. Total 73 listed literature were classified by compound analysis and pharmacological efficacy. Results : C. setidens contains pectolinarin and its glycoside, pectolinarigenin as index compounds, and linarin, apigenin, diosmetin, scopoletin, acacetin, cirsimarin, cirsimaritin, setidenosides A and B, silymarin, hispidulin, 92 volatile compounds, and 15 fatty acids. The Pharmacological activities of C. setidens has been reported to inhibit of platelet aggregation and fat accumulation in the liver, inhibit to hepatitis, anti-cancer, antibacterial, skin improvement, hair growth, liver protection, anti-diabetic, anti-inflammatory, sedative. Also, It has been reported the effect of cholesterol-lowering and anti-obesity, neuroprotective effects, increasing human stem cell viability, inhibiting osteoclast formation and osteogenic differentiation. Conclusion : This reviews showed that C. setidens which has been traditionally used for the treatment of inflammation and hypertension, has anticancer and river protective effect, as well as hair loss and diet. In order to maximize the efficacy of C. setidens, research has also begun on the effect of processing processes such as fermentation or fine powdering and combining natural plant resources.

미래의 학교가 요구하는 중등교원 양성체제

조미애 참마음교육학회 2006 학술대회 자료집 Vol.16 No.-

국내 옥수수 순계주에서 CP4 5-Enol- Pyruvylshikimate-3- Phosphate Synthase 유전자의 발현

조미애,권석윤,김진석,이병규,문추연,최필선,Cho, Mi-Ae,Kwon, Suk-Yoon,Kim, Jin-Seog,Lee, Byoung-Kyu,Moon, Choo-Yeun,Choi, Pil-Son 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4

국내 옥수수 순계주에서 Agrobacterium 공동배양으로 CP4 5-Enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) 유전자가 도입된 제초제저항성식물체를 개발하였다. 5개의 순계주 (HW1, KL103, HW3, HW4, HW7)의 미숙배를 Ubiquitin promoter-CP4 EPSPS 유전자와 CaMV35S promoter-nptII 유전자가 발현되도록 제조된 pCAMBIA2300 벡터를 C58C1 Agrobacterium에 형질전환하여 공동 배양하였다. 항생제로 paromomycin이 첨가된 배지에서 선발된 옥수수 형질전환체를 PCR, RT-PCR 및 Northern 분석을 통하여 유전자의 도입과 발현을 확인하였다. 또한 형질전환 식물체의 glyphosate 처리에 따른 shikimate 축적반응을 확인하였다. Paromomycin 저항성 캘러스 형성빈도는 옥수수 각 순계주 HW1, KL103, HW3, HW4, HW7에서 각각 0.37%, 0.03%, 2.20%, 2.37%, 0.81%로 나타났으며, PCR분석을 통하여 최종적으로 2개의 옥수수 순계주 (HW3, HW4)의 paromomycin 저항성 캘러스로부터 분화된 식물체에서 확인하였다. 이러한 형질전환체중에서 RT-PCR 및 Nothern blot 분석을 통하여 CP4 EPSPS 유전자가 발현되는 2개의 계통 (M266, M104) 을 선발하였고, shikimate 축적반응을 통하여 glyphosate에 대한 저항성을 갖는 계통 (M266)을 최종적으로 선발하였다. 이러한 결과는 국내 옥수수 순계주에서 제초제저항성을 갖는 옥수수 형질 전환체를 개발할 수 있음을 시사한다. This study was conducted to develop herbicide-resistance domestic maize plants by introducing the CP4 5-enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) gene using Agrobacterium tumefaciens-mediated immature embryo transformation. Immature embryos of five genotypes (HW1, KL103, HW3, HW4, HW7) were co-cultivated with strains Agrobacterium tumefaciens (strain C58C1) containing the binary vector (pCAMBIA2300) carrying Ubiquitin promoter-CP4 EPSPS gene and Cauliflower mosaic virus 35S (CaMV35S) promoter-nptll gene conferring resistance to paromomycin as a selective agent. The presence and expression of CP4 EPSPS transgene were confirmed by PCR, RT-PCR and Northern blot analysis, respectively. Also, the resistance to glyphosate in the transgenic maize ($T_1$) was analyzed by shikimate accumulation assay. The frequency (%) of paromomycin-resistance callus was 0.37, 0.03, 2.20, 2.37, and 0.81% in pure lines HW1, KL103, HW3, HW4 and HW7, respectively. EPSP transgene sequences were amplified in putative transgenic plants that regenerated from paromomycin-resistance calli of two inbred lines (HW3, HW4). Of them, RT-PCR and Northern blot analyses revealed that the transgene was only expressed in two transgenic events (M266, M104) of HW4 inbred line, and a mild glyphosate resistance of transgenic event (M266) was confirmed by the lower shikimate accumulation in leaf segments. These results demonstrate that transgenic maize with herbicide-resistance traits in Korean genotype can be genetically obtained.