Vanadium이 신장 Na-K-ATPase에 미치는 영향

김인순,이상복,조규철,Kim, I.S.,Lee, S.B.,Cho, K.C. 대한약리학회 1983 대한약리학잡지 Vol.19 No.1

Vanadium is widely distributed in animal tissues and it is supposed to be a regulator of Na-K-ATPase activity. The effect of sodium orthovanadate on Na-K-ATPase activity in rabbit kidney was measured in vitro and compared with that of ouabain. The influence of sodium orthovanadate on the renal function of rabbits was also investigated. 1) Na-K-ATPase activity was decreased by sodium orthovandate at the concentrations of $10^{-7},\;10^{-6},\;10^{-5}\;and\;10^{-4}\;M$ to 73.89, 36.49, 6.50 and 4.99% of the control activity respectively. 2) Na-K-ATPase activity was decreased by ouabain at the concentrations of $10^{-4},\;10^{-3}\;and\;10^{-2}\;M$ to 69.52, 22.84 and 3.88% of the control activity respectively. 3) Urine volume, urinary excretion of $Na^+,\;K^+\;and\;Cl^-$, clearances of inulin and p-amino-hoppuric acid were decreased until after 60 minutes following the administration of sodium orthovanadate 0.5 mg/kg intravenously $Na^+\;reasorption$ rate was not changed and mean arterial pressure was significantly elevated during 60 minutes after the administration of sodium orthovanadate.

수종 생약제가 신기능 및 신장 $Na^+-K^+$ ATPase에 미치는 영향

김인순,염윤희,이상복,조병헌,조규철,Kim I.S.,Yeom Y.H.,Lee S.B.,Cho B.H.,Cho K.C. 대한약리학회 1980 대한약리학잡지 Vol.16 No.1

Phytolaccae Radix (PR), Brunella Herba (BH), Akebiae Lignum (AL) and Atractylis Rhizoma (AR) are some of the diuretic agents used in Chinese medicine and folk remedy. Water or methanol extracts of them (100mg/kg) were intravenously injected to rabbits in order to re-evaluate the effects on renal function. PR water extract elicited moderate diuresis while water extracts of BH, AL and methanol extract of AR had antidiuretic effects. Influence of PR on renal hemodynamics and $Na^+-K^+$-ATPase activity in rabbit kidney were observed in vivo and in vitro. The results were as follows: 1) Clearances of inulin and p-aminohippuric acid increased significantly after 15 minutes following the administration of PR water extract, but Na+ reabsorption rate was not changed. 2) The increase of $Na^+-K^+$-ATPase activity in renal cortex, outer and inner medulla was observed at 15 minutes after PR water fraction was given intravenously, and the change was most prominent in cortical area. 3) More than 50% of decrease in $Na^+-K^+$-ATPase activity in renal tissues was observed with PR water fraction $(10^{-2}g/ml)$ in vitro experiments. However, the inhibition of $Na^+-K^+$-ATPase activity was reversed with lower concentrations $(10^{-4}g/ml,\;10^{-6}g/ml)$ of PR water fraction in outer and inner medullary zone. These results suggest the diuretic effect of PR is due to improved renal hemodynamics, and contradictory reults concerning $Na^+-K^+$-ATPase activity require further investigation.

Penicillin G 주입이 Furosemide 이뇨작용에 미치는 영향

김인순,이상복,조규철,Kim I.S.,Lee S.B.,Cho K.C. 대한약리학회 1980 대한약리학잡지 Vol.16 No.2

The influence of penicillin G infusion(7.5 units/min/kg) on the diuretic action of furosemide$(250{\mu}g/kg)$ in rabbits was studied to investigate the drug interaction between penicillin G and furosemide. The results were as follows: 1) There was no significant change in renal function in the penicillin G infused rabbits compared with the normal ratbit. 2) In comparison with the control group, a significant increase in ${\triangle}U_{flow},\;{\triangle}U_{Na}V,\;{\triangle}U-kV\;and{\triangle}U_{cl}V$ was noted by 30 minutes following the administration of furosemide in the penicillin G infused group. There was no significant difference in ${\triangle}C_{In},\;{\triangle}C_{PAH}$ between the two groups and ${\triangle}Na^+$ reabsorption rate was significantly decreased in the latter. The potentiating effect of penicillin G on the diuretic action of furosemide should be due to the increased luminal concentration of free form of furosemide, and it may be related to the competitive inhibition of plasma protein binding between the two drugs.

황련성분의 혈압하강 작용

곽인숙,이상복,조병헌,조규철,Kwak I.S.,Lee S.B.,Cho B.H.,Cho K.C. 대한약리학회 1976 대한약리학잡지 Vol.12 No.2

Coptis rhizoma had been applied in the chines medicine as well as in the folk remidies and it was advocated that coptis rhizoma exerts good therapeutic effect in some case of alimentary disorders and other hypotensive ailments. However the basic pharmacology and the mechanism of hypotensive action are not clear. This experiment was carried out in order to reevaluate the pharmacology, especially hypotensive action of coptis rhizoma and to clarify the mechanism of action, in the rabbits and also $LD_{50}$ of coptis rhizoma was determined using mice as experimental animals. The results of the experiment were as follows. 1) $LD_{50}$ to the mice was 69 mg/kg. 2) Blood pressure manifested gradual responses by the fall of -17, -26, -62 mmHg in proportion to the administration of 3, 5, 10 mg/kg of coptis rhizoma extract respectively. 3) No significant differance was observed in heart rate between the normal control group and the coptis rhizoma extract treated group. 4) Administration of coptis rhizoma extract 5 mg/kg to the rabbit pretreated with atropine or avil did not show any significant difference in the blood pressure compared with that of control group. 5) In the rabbit pretreated with coptis rhizoma extract 5mg/kg, the hypotensive effect of epinephrine was significantly inhibited in comparison with that of normal rabbit.

심혈관계의 중추조절에 대한 Prostaglandin $F_{2{\alpha}}$의 영향

이상복,김인순,김옥녀,조규철,Lee, S.B.,Kim, I.S.,Kim, O.N.,Cho, K.C. 대한약리학회 1982 대한약리학잡지 Vol.18 No.1

This study was carried out in order to clarify whether the cardiovascular effect of prostaglandin(PG) $F_{2{\alpha}}$ might be centrally mediated. In unrestrained conscious rat, $PGF_{2{\alpha}}$ was administered into the lateral ventricle. The mechanism of action was also studied by observing the interaction with several adrenergic antagonists injected subcutaneously, Indomethacin was administered into lateral ventricle to investigate the role of endogenous $PGF_{2{\alpha}}$ on the central regulation of cardiovascular system. The results were as follows: 1) The intraventricular injection of $PGF_{2{\alpha}}$ produced an increase in blood pressure and heart rate. 2) The pretreatment with phenoxybenzamine (2 mg/g, s.c.) inhited pressor, but not heart rate responses to the intraventricular injection of $PGF_{2{\alpha}}$ $(2{\mu}g/kg)$. 3) The pretreatment with propranolol (1 mg/kg, s.c.) inhibited tachycardia, but not pressor responses to the intraventricular injection of $PGF_{2{\alpha}}(2{\mu}g/kg)$. 4) The intraventricular injection of indomethacin $(40{\mu}g/kg)$ could not induce significant changes in blood preesure and heart rate. 5) The result indicates that intraventricular injection of $PGF_{2{\alpha}}$ produces pressor and tachycardia responses in the unanesthetized rat, and it is mediated primarily by centrally increased sympathetic outflow. But the endogenous $PGF_{2{\alpha}}$ synthetized in the brain seems to play minor role in the direct regulation of cardiovascular system.

한국산 생약제들의 혈압강하작용에 대한 연구 II. 한국산 후박수피의 혈압강하 작용

조병헌,김인호,이상복,조규철,이종화,Cho B.H.,Kim I.H.,Lee S.B.,Cho K.C.,Lee J.H. 대한약리학회 1979 대한약리학잡지 Vol.15 No.1

With a view to searching after a new antihypertensive or hypotensive agents in the botanical crude plants, authors intended to reevaluate several natural products caltivated in Korea. This experiment was undertaken to compare pharmacogical actions of Machilus thunbergii Siebold et Zuccarini with those of Magnolia obovata Thunberg in anesthetized rats and in normal mice. Machilus thunbergii Sieb. et Zucc., a tree belonging to the Lauraceae family, is caltivated at Ull-ung Do, and their cortecies have been used as folk medicine mingled with those of Magnolia obovata Thunberg. These two cortecies have teen also applied in chinese medicine, it was advocated that these cortecies exerted good therapeutic effects on gastritis, convulsive abdominal pain, nausea, vomiting and urinary tract disorders. Therefore, we intended to determine the pharmacological action of two palnt of different family each other, especially their effects on blood pressure and heart rate, and also their mechanism of action were observed. We studied their action with extracts of hexane(MTHE), ether(MTEE), methanol(MTME) and water(MTWE) from Machilus thunhergii Sieb. et Zucc., and also fractionations of methanol(MOME), chloroform(MOCE) and water(MOWE) from Mapolia obovata Thunberg. The results of this experiment were as follows; 1) MTME, when intravenously administered to rats, elicited the significant hypotensive responses dependent on the administered dosage. 2) MOWE was also exhibited the hypotensive effect dependent on the treated dose. 3) Depressor effect of MTME was blocked by pretreatment with hexamethonium. 4) The hypotensive response of MOWE was blocked by pretreatment with hexamethonium or hrdralazine. 5) HTME and MOWE were also observed the anticonvulsive effect and sedative effect. These results suggested that MTME may induce the hypotensive response via central sympathetic effect, but the site of action in brain are not clarified, and the hypotensive effect of MOWE may be due to dual mechanism of central sympathetic action and direct vasodilation of blood vessel.

토끼의 혈장내 Aldosterone 농도에 미치는 Aspirin과 Furosemide의 영향

서영진,이권행,김옥녀,이상복,조규철,Suh, Y.J.,Lee, K.H.,Kim, O.N.,Lee, S.B.,Cho, K.C. 대한약리학회 1984 대한약리학잡지 Vol.20 No.2

It has been generally recognized that the secretion of aldosterone is mainly regulated by angiotensin II in animals and humans, however, potassium and ACTH are also proposed as other humoral factors involved in the aldosterone secretory process. Recently, stress, anesthesia, adrenergic stimulation, low sodium intake or water deprivation stimulate plasma renin activity, while high sodium intake and deoxycorticosteroid have been reported to cause suppression of renin activity in animals. It seems that overall response of aldosterone secretory mechanisms reflects complex interactions both intrarenal and extrarenal components. Furosemide has been widely used to investigate the control of renin secretion by the kidney, and the relationship between diuretics and the disposition of endogenous aldosterone were reported (Oh, 1984). The sequential with 10 min interval samples of plasma were collected following administration of furosemide(1 mg/kg), aspirin(10 mg/kg), respectively. And also similar experiment was performed in the propranolol (10 mg/kg) pretreated rabbits. The results were as follows : 1) The concentration of plasma aldosterone was average of $426.I{\sim}485.5pg/ml$ in normal rabbits. Plasma concentrations of aldosterone rised significantly after injection of furosemide during 50 min, and the rise of plasma aldosterone was blocked by the propranolol pretreatment 2) Significant fall in the plasma level of aldosterone after injection of aspirin was noted. This result indicates that the increased secretion of aldosterone induced by furosemide administration is mediated through ${\beta}-receptors$, and the possible role of prostaglandin is substantiated.

심혈관계의 중추조절에 대한 Prostaglandin F_2α의 영향

이상복(S.B. Lee),김인순(I.S. Kim),김옥녀(O.N. Kim),조규철(K.C. Cho) 대한약리학회 1982 대한약리학잡지 Vol.18 No.1

This study was carried out in order to clarify whether the cardiovascular effect of prostaglandin(PG) F_2α might be centrally mediated. In unrestrained conscious rat, PGE_2α was administered into the lateral ventricle. The mechanism of action was also studied by observing the interaction with several adrenergic antagonists injected subcutaneously, Indomethacin was administered into lateral ventricle to investigate the role of endogenous PGE_2α on the central regulation of cardiovascular system. The results were as follows: 1) The intraventricular injection of PGE_2α produced an increase in blood pressure and heart rate. 2) The pretreatment with phenoxybenzamine (2 mg/g, s.c.) inhited pressor, but not heart rate responses to the intraventricular injection of PGE_2α (2μg/kg). 3) The pretreatment with propranolol (1 mg/kg, s.c.) inhibited tachycardia, but not pressor responses to the intraventricular injection of PGE_2α(2μg/kg). 4) The intraventricular injection of indomethacin (40μg/kg) could not induce significant changes in blood preesure and heart rate. 5) The result indicates that intraventricular injection of PGE_2α produces pressor and tachycardia responses in the unanesthetized rat, and it is mediated primarily by centrally increased sympathetic outflow. But the endogenous PGE_2α synthetized in the brain seems to play minor role in the direct regulation of cardiovascular system.

흰쥐의 신조직내 Cyclic Nucleotide함량에 대한 Aspirin, Indomethacin 및 Furosemide의 상호작용

이석용,이권행,김옥녀,이상복,조규철 최신의학사 1985 最新醫學 Vol.28 No.6

한국산 생약제들의 혈압하강작용에 대한 연구 : Ⅰ. 황련·택사·오가피

이상복,김옥녀,조병현,조규철 최신의학사 1978 最新醫學 Vol.21 No.4

For the purpose of searching the new antihypertensive agent in the botanical crude drugs, authors intended to determine whether several crude drugs might manifest the hypotensive effect and to clarify the mechanism of this hypotensive agent. This experiment was studied on the hypotensive action with Coptis rhizoma crystal(CR), ethanol extract Alismatis rhizoma (AREE) and ethanol extract of Acanthopanis cortex radicis (ACEE) in the rat. The results of the experiment were as follows: 1) Blood pressure manifested gradual responses by the fall of ?28.0mmHg, -64.5mmHg and -84.0mmHg in proportion to the intravenous injection of 2 mg/kg, 5mg/kg and l0mg/kg of CR respectively. 2) The intravenous injection of 50mg/kg and EOmg/kg of AREE manifested the fall of blood pressure as -51.2mmHg and -56.6mmHg respectively. 3) The intravenous injection of 40mg/kg and 80mg/kg of ACEE manifested the fall of blood pressure as -35.5mmHg and -53.5mmHg respectively. 4) The administration of epinephrine 50mg/kg to the rat pretreated with CR 5mg/kg revealed "epinephrine reversal action" by the fall of blood pressure. 5) The administration of AREE 50mg/kg to the rat pretreated with atropine or diphenhydramine inhibited significantly the hypotensive action of AREE slightly. 6) The administration of ACEE 80mg/kg to the rat pretreated with diphenhydramine inhibited the hypotensive action of ACEE significantly.