http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
박예슬(Ye Seul Park),남건희(Gun He Nam),조경조(Kyung Jo Jo),곽혜원(Hye Won Kawk),김명진(Myeong Jin Kim),김종태(Jong Tae Kim),장승희(Seung Hee Jang),김민정(Min Jeong Kim),김영민(Young Min Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
Obesity is caused by a variety of reasons, such as diet, genetics, and environmental factors. Here the effect of Barley sprout extract on the differentiation of 3T3-L1 and the expression of adipogenesis was studied. MTT assay for cell toxicity was performed and found to be non-toxic at 3T3-L1, with a survival rate of 90% or higher in Barley sprout extract at all concentrations. The results of oil red O assay, after inducing differentiation by treating the fat formation with insulin, dexamethasone, and IBMX powder-inducing substances at 3T3-L1, showed that the Barley sprout extract inhibited fat formation. The results of a triglyceride assay for the determination of quantitative inhibition of fat formation showed 42.2% and 45.7% in Barley sprout extracts of 50% and 70%, respectively. Western blot was performed to identify the proteins involved in fat cell suppression, and it was found that there was a concentration-dependent decrease in C/EBPα, PPARγ, and p-ACC proteins. Thus, Barley sprout ethanol extract regulates the expression of protein involved in inhibiting fat cells, thereby reducing the differentiation of fat cells and triglyceride production and indicating that it is effective against obesity.
Ye Seul Park(박예슬),Gun He Nam(남건희),Kyung Jo Jo(조경조),Hye Won Kawk(곽혜원),Je-Geun Yoo(유제근),Jin Dong Jang(장진동),Sang Moon Kang(강상문),Sang Yong Kim(김상용),Young Min Kim(김영민) 한국생물공학회 2019 KSBB Journal Vol.34 No.3
Collagen is decomposed by MMP-1, an enzyme induced by transcription factor activator protein 1 (AP-1) and matrix metalloproteinase-9 (MMP-9). Action of MMPs in inflammatory response promotes inflammatory cell movement and secretion, resulting in wrinkles on the skin. After using Bacillus sp. fermentation system and water, Artemisia vulgaris was fermented to prepare different solvent fractions using water, dichloromethane, hex ane, n-butanol, and ethyl acetate. These fractions were used to assess their effects on cell survival, wound healing, MMP-1/MMP-9 and procollagen type I C-peptide (PICP) expression, and skin turnover. MTT assay showed that cell viability of each treated group was 103% to 121%, indicating that A. vulgaris fractions were not toxic compared to control (cell viability: 100%). Wound healing assay revealed that wound healing ability in each treated group was 51% to 61%. This was lower than wound healing area in the control. Using RT-PCR, inhibition of MMP-1/MMP-9 gene expression was examined. As a result, each group treated with fraction showed reduced expression of both MMP-1 and MMP-9 compared to tumor necrosis factor-α (TNF-α) treatment group. Effects on collagen biosynthesis were analyzed using a PICP ELISA Kit. The group in which Artemisia vulgaris was treated increased collagen synthesis from 141 to 262ng/mL compared to the control group. Three-dimensional cell culture revealed that each fraction could increase skin wall formation. These results suggest that each fractions has anti-aging and anti-wrinkle effects on the skin, indicating its suitability as a functional material.
주정 중독 흰쥐에서 총담관 결찰이 혈청 및 간의 Υ-Glutamyl Transpeptidase 활성에 미치는 영향
조경일,김여희,곽춘식 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.2
이 연구는 간담도 질환시 음주의 해로움에 대한 생화학적 배경의 일단을 밝히고자 시행한 실험으로서 급성 및 만성 주정 중독을 시킨 흰쥐에게 총담관결찰로 담즙울체를 야기시켜 혈청과 간의 γ-glutamyl transpeptidase(γ-GTP) 활성도를 측정한 것이다. 흰쥐에게 만성 주정 중독을 시켰을 때 간의 microsomal γ-GTP 활성도는 유의한 증가를 나타내었다. 만성 주정 중독 후 총담관을 결찰하여 담즙울체를 야기시켰을 때는 혈청과 간의 microsomal γ-GTP 활성도는 총담관만 결찰시켰을 때 보다 현저한 증가를 나타내었다. 그러나 간의 microsomal γ-GTP 활성도는 유의한 감소를 나타내었다. 총담관 결찰 후 급성 주정 중독을 시켰을 때는 간의 microsomal γ-GTP 활성도는 총담관만 결찰시켰을 때보다 현저한 감소를 나타내었다. 이상의 성적으로 보아 간의 microsomal γ-GTP는 만성 주정 중독시 담즙울체를 야기했을 때는 그 합성이 담즙울체만 있을 때보다 증가되는 효소로 생각된다. 그러나 간의 microsomal γ-GTP는 급성 및 만성 주정 중독시 담즙울체가 야기되면 담즙울체만 있을 때 보다 그 합성이 감소되는 효소로 생각된다. 특히 만성 주정 중독시 담즙울체가 야기되면 담즙울체만 있을 때보다 간손상이 증폭되므로 간에서 혈증으로 이 효소의 누출이 증가되는 것으로 생각된다. The activities of the serum and liver Υ-glutamyl transpeptidase(Υ-GTP) were studied in cholestasis induced by common bile duct(CBD) ligation and chronic ethanol intoxication and in cholestasis before acute ethanol intoxication to establish the biochemical background of alcoholic hazard in hepatobiliary disease. There was a significant increase in the hepatinc microsomal Υ-GTP activity of rats treated with only chronic ethanol intoxication. In the group with CBD ligation after chronic ethanol intoxication, the activities of serum and hepatic microsomal Υ-GTP showed a significant decrease than in the group with only CBD ligation. On the other hand, in the group with CBD ligation before acute ethanol intoxication, the activity of hepatic mitochondrial Υ-GTP decreased more markedly than in the group with only CBD ligation. According to the above results, the liver microsomal Υ-GTP seems to be and enzyme which increase in its activity in chronic ethanol intoxication with cholestasis more than in cholestasis. And the liver mitochondrial Υ-GTP seems to be and enzyme which decrease in its activities in both acute and chronic ethanol intoxication with cholestasis more than in cholestasis. The causes of the increase or the decreases seem to be the development or the retardment of biosynthesis. Especially, when the chronic ethanol intoxication with cholestasis occurred, the activity of serum Υ-GTP was higher than that of the cholestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity.
만성 주정 중독 흰쥐에서 총담관 결찰이 UDP-Glucuronosyltransferase 활성에 미치는 영향
조경일,김여희 啓明大學校 醫科大學 1999 계명의대학술지 Vol.18 No.3
The alcoholic hazard in hepatobiliary disease was studied by the assays of liver microsomal UDP-glucuronosyltransferase activity in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication in rats. The microsomal UDP-glucuronosyltransferase activity and the Vmax value of the enzyme in the cholestatic rat liver combined with chronic ethanol intoxication showed further significant decrease compared to the activity from cholestasis alone. However, the Km value of th enzyme did not change. The results indicate that the biosynthesis of the liver UDP-glucuronosyltransferase is decreasing in chronic ethanol intoxication with cholestasis than in cholestasis alone.
만성 주정 중독 쥐에서 총담관 결찰이 간의 Aryl Sulfotransferase Ⅲ,Ⅳ 의 활성에 미치는 영향
조경일,김여희,곽춘식 계명대학교 의과학연구소 2001 계명의대학술지 Vol.20 No.1
The drinking hazard in hepatobiliary disease was studied by the assays of hepatic aryl sulfotransferase Ⅲ, Ⅳ isozyme activies in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication in rats. The activity of serum aryl sulfotransferase Ⅲ, Ⅳ isozyme was also measured. Liver cytosolic aryl sulfotransferase Ⅲ, Ⅳ isozyme activity in the group of CBD ligation after chronic ethanol intoxication showed a greater decrease between the 7th and the 14th day after CBD ligation than that in the group of CBD ligation alone. The mitochondrial aryl sulfotransferase Ⅲ, Ⅳ isozyme activity in the group of CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the group of CBD ligation alone. The microsomal arylsulfotransferase Ⅲ, Ⅳ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater decrease between the 2nd and 14th day after CBD ligation than that in the group of CBD ligation alone, The serum aryl sulfotrnasferase Ⅲ, Ⅳ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater increase between the 1st and 14th day after CBD ligation than that in the group of CBD ligation alone. The Vmax values of the liver cytosolic and mitochondrial aryl sulfotransferase Ⅲ, Ⅳ isozyme in the CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the group of CBD ligation alone. However, the Km values of the above hepatic enzymes did not change. The results indicate that the biosynthesis of the liver aryl sulfotransferase Ⅲ, Ⅳ isozyme is decreasing in chronic ethanol Intoxication with cholestasis than in cholestasis alone. The increased activity of the serum aryl sulfotransferase Ⅲ, Ⅳ isozyme in chronic ethanol intoxication with cholestasis reflects increased hepatic damages which cause this isozyme to leak into the blood in great quantity.
만성 주정 중독 쥐에서 총담관 결찰이 간의 Aryl Sulfotransferase I,II Isozyme의 활성에 미치는 영향
조경일,곽춘식 계명대학교 의과학연구소 2000 계명의대학술지 Vol.19 No.1
The drinking hazard in hepatobiliary disease was studied by the assays of liver cytosolic, mitochondrial, and microsomal aryl sulfotransferase Ⅰ, Ⅱ isozyme, in cholestasis induced by common bile duct (CBD) ligation after chronic ehtanol intoxication with rats. The activity of serum aryl sulfotransferase Ⅰ, Ⅱ isozyme was also measured. Liver mitochondrial aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in group of CBD ligation after chronic ethanol intoxication showed a greater decrease between the 3rd and the 14th day after CBD ligation than that in group of CBD ligation alone. However, the cytosolic Ⅰ, Ⅱ isozyme activity did not change. The nicrosomal aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in the CBD ligation after chronic ehanol intoxication showed a greater decrease between the 7th and the 14th day after CBD ligation than that in the CBD ligation alone. The serum aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater increase between the 2nd and the 14th day after CBD ligation than that in the CBD ligation alone. The Vmax values of the liver mitochondrial and microsomal aryl sulfotransferase Ⅰ, Ⅱ isozymes in the CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the CBD ligation alone. However, the Km values of the above hepatic enzymes did not change. The results indicate that the biosynthesis of the hepatic aryl sulfotransferase Ⅰ, Ⅱ isozyme is decreasing in chronic ethanol intoxiaction with cholestasis than is cholestasis alone. The increased activity of the serum aryl sulfotransferase Ⅰ, Ⅱ isozyme is chronic ethanol intoxication with cholestasis than in cholestasis alone reflect that increased hepatic damages which cause this isozyme to leak into the blood in great quantity.