체외수정 및 미세조작에 의한 가축배의 생산과 효율적 이용에 관한 연구 6. 소 핵이식배의 체외배양 , 동결보존 및 성판정에 관한 연구

정영채,김창근,윤종택,나광빈,오성종,이종완,김흥률,김광식,박선애,유영아 ( Y . C . Chung,C . K . Kim,J . T . Yoon,G . B . Luo,S . J . Oh,J . W . Lee,H . R . Kim,K . S . Kim,S . A . Park,Y . A . You ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3

This study was carried out to establish an effective method for the improvement of subsequent development after nuclear transfer(NT) in bovine embryo, for the extension of utility through freezing and sexing of NT embryos. The rates of cleaved and developed embryos upto morula-blastocyst stages after the NT in in vitro produced embryos were 60.6 and 3.7%, respectively. Whereas, in in vivo embryos, slightly higher developments were obtained(70.8 and 5.6%), respectively. No difference was found in embryonic development upto morulablastocyst stages among the different levels of EGF added to the serum containing medium. The rates of cleaved and developed embryos upto morula-blastocyst were higher when electrostimulation was performed either before fusion or after addition at 21h IVM. The survival rate of frozen NT embryos was higher in late morula than that of earlier embryos. PCR-based sexing of NT embryo demonstrated that higher proportion of embryos was male(1.9:1), instead of normal ratio(1:1).

한우와 샤로레 교잡종 빈우의 성성숙과 성호르몬 수준 변화에 관한 연구

정영채,김창근,이근상,Chung Y. C.,Kim C. K.,Lee K. S. 대한수의사회 1982 대한수의사회지 Vol.18 No.7

한우와 Charolais와의 교잡종 빈우에 있어서 초발정일령과 체중 및 성장중의 혈중 LH, FSH prolactin, progesterone과 estradiol수준의 변화를 알고저 본 시험을 시도하였다. 공시된 빈우는 한우 4두와 교잡종$(F_1)$, 4두로서 NRC 사용표준에 따라 사료를 급여하였고 군사하였다. 체중은 출생일로부터 1개월 간격으로 16개월령까지 측정하였으며 13개월령까지 체중측정시마다 채혈한 다음 분리된 혈청내의 호르몬을 RIA로 분석하였다. 초발정일령은 유의성이 없었으나 교잡종이 한우보다 빨랐다($326.7{\pm}51.6$일과 $372.5{\pm}31.9$일) 초발정시 체중은 교잡종이 한우보다 월등히 (p<0.01) 무거웠다 ($223.0{\pm}16.6kg$과 $175.3{\pm}10.3kg$). 혈청내 LH와 FSH는 1두의 교잡종에서 출생시와 3개월령에서 높게 나타난 것을 제외하고는 전빈우의 전월령에서 본 시험에서의 호르몬분석한계수준(LH, $1.5mIU/m\ell$ : FSH, $1.2mIU/m\ell$) 이하였다. 혈청내 prolactin은 다른 월령에서 보다 $5\~8$개월령에서 더욱 높았고 $11\~12$개월령에서는 개체간 차이가 많았다. 혈청내 progesterone은 출생시는 낮았으며 그 후 $6\~8$개월령에서 높았고 8개월령 이후에서 $0.1\~0.2ng/m\ell$으로 다시 감소하였다. 혈청내 estradiol수준은 전빈우의 모든 월령에서 본 시험의 호르몬분석 한계수준$(10pg/m\ell)$ 이하였다. 성성숙동안에 LH, FSH와 estradiol의 수준이 낮았던 원인은 이들 호르몬의 상승을 측정하기에는 너무 채혈간격이 길었던 것으로 추측된다. This experiment was conducted to determine the age and weight at first estrus and to characterize the serum LH, FSH, prolactin, progesterone and estradiol during growth and puberty in Korean native heifers and Charolais x native crossbred heifers. Four pu

생명과학을 위한 실험동물과 수의사의 역할

정영채,Chung Yung Chai 대한수의사회 1977 대한수의사회지 Vol.13 No.5

시내요식업소 식품 및 환경위생학적조사 -주로 대장균이 검출을 위하여-

정영채,CHUNG YUNG CHAI 대한수의사회 1963 대한수의사회지 Vol.7 No.1

During the period starting November 8th to 26th, 1960, 160 restaurunts located in Seoul area were inspected with special emphasis on the sanitary aspect of food and environment of each restaurant. The inspection was concerned with bacteriological test for

하수체척출 흰쥐에 있어서 성Hormone 의 투여가 갑상선에 미치는 영향

정영채,이규승,김관영 ( Y . C . Chung,K . S . Lee,K . Y . Kim ) 한국축산학회 1974 한국축산학회지 Vol.16 No.2

The study was made to investigate whether there would be any direct relationship between. sexual gland and thyroid gland without the control mechanism from the hypophysis. After the administration of sex hormone to the albino rats (hexosterol to female and testosterone propionate to male), weights of thyroid glances, the uptake rate of radioactive iodine-131 (^(131)I) and histological changes were measured from the 1st day to 56th day cf the experimental period. The results obtained were as fo[lows; 1. For the weight changes cf thyroid gland, it shoved a similar changes between the hypophysectomized group and the sex hormone treated-hypophysectomized group. There were no sexual differences among the groups. However, the weights of thyroid gland for the treatment groups mere decreased as the time passed as compared to the control group, and the differences were highly significant at the 7th day and there on. 2. For the uptake rates of radioisotope-^(131)I the changes were similar between both sexes, but were decreased rapidly as time passed. The differences were significant at the first day and highly significant at the 7th day and there on for the hypophysectomized group. The changes for the sex hormone treated-hypophysectomized group were also similar to the hypophysectomized group. 3. In the histological changes of the thyroid gland, there mere no sexual differences among the groups. However, there were similar changes in both hypophysectomized groups and sex hormone treated-hypophysectomized group, showing slight squamous sign in follicular epithelial cells at the 7th day, but considerable degree of squamousl sign accompained with pyknosis of the cell and irregular enlargement of the follicles at the 14th day following the treatment. At the 28th day, it seemed that the degeneration was much progressed, and at the 43nd and 56th day, it showed karyorrhectic sign of follicular epithelial roils and therefore the loss of secretory function, histologically. 4. Since there were no differences in weight changes of thyroid gland, the uptake rates of radioisotope-^(131)I and histological change between the hypophysectomized and sex hormone treated-hypophysectomized groups, it would appear that there were no direct relationship between the sexual gland and the Thyroid gland. but the involvement of hypophysis might be necessary for the control mechanism.

방사성옥도(放射性沃度)(I¹³¹)의 주입량(注入量)이 기니픽 갑상선(甲狀腺)에의 섭취(攝取) 및 방출률(放出率)에 미치는 영향(影響)에 관(關)한 연구(硏究) 제(第)1보(報) 방사성옥도(放射性沃度)(I¹³¹)의 주입량(注入量)이 웅(雄)기니픽 갑상선(甲狀腺

정영채,심상칠,이흥식,Chung, Yung Chai,Shim, Sang Chil,Lee, Heung Shik 대한수의학회 1966 大韓獸醫學會誌 Vol.6 No.1

In order to observe the effect of the injected radioactive iodine-$I^{131}$ on the uptake in thyroid of normal male guinea pigs and P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum, 24 matured male guinea pigs were divided in 4 groups and $35{\mu}c$, $70{\mu}c$, $140{\mu}c$ and $280{\mu}c$ per kg of body weight respectively were injected subcutaneously. 1. The uptake rates of radioactveiodine-$I^{131}$ by external counts of thyroidal uptake reached the maximum level of uptake in 24 hours after injection. 2. As the injected amount increases, the uptake rates of maximum levels and release rate were increased. 3. Uptake rate in the removed thyroid have shown no statistical in the $35{\mu}c$ and $70{\mu}c$ groups of injected guinea pigs. 4. There was no statistical significance in $140{\mu}c$ and $280{\mu}c$ groups of injected guinea pigs. 5. P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum was not in proportion to injected amounts: 61.0%(35), 70.2%(70), 75.3%(140) and 64.8%(280).

일본뇌염(日本腦炎)에 대(對)한 한국가축(韓國家畜)에서의 혈청학적(血淸學的) 조사(調査) 연구(硏究)

정영채,문재봉,강병직,권혁진,최희인,Chung, Y.C.,Moon, J.B.,Kang, B.J.,Kwon, H.J.,Choi, H.I. 대한수의학회 1971 大韓獸醫學會誌 Vol.11 No.2

Since the report in 1946 on the first isolation of Japanese encephalitis virus in Korea, the disease is known to occur every year and has occasionally spread in epidemic proportion among human populations. Because of its public health importance, it was deemed highly desirable to gain specific information as to natural history of the disease in Korea if we are to accomplish our ultimate objective of controling the disease in Korea. There still remain, however, unknown factors as to the ecology of Japanese encephalitis virus in Korea. This paper presents the results of serologic study on Korean cattle, hog and horse by means of hemagglutination inhibition (H.I.) test. The serum specimens were collected from Korean cattle, hog and horse their estimated ages being 3 to 9 years, 6 to 24 months and 2 to 14 years respectively, by jugular puncture; in Seoul and Kyung-Ki are a from the first part of May to November last, 1968. The strain used was designated by M 5/596. The methods of H.I. test employed in this study were essentially simillar to those employed by Clark and Fred. The results obtained summarized as follows; 1. Of samples of cattle sera tested, 183 (98%) was found to be positive, H.I. antibodies showing the highest proportion, on September. The lowest proportion showed 81 (68%) of 117 samples, on June. 2. One hundred and ninety-five and 114 swine serum samples tested were all of positive in both on September and October respectively. The lowest proportion showed 92 (29%) of 314 on June too. 3. Ninety (99%) of 91 equine serum samples tested contained demonstrable H.I. antibodies the highest proportion, on August. The lowest proportion was 19 (27%) of 70 samples on June. 4. Implications of these findings in the ecology of Japanese encephalitis Seoul and Kyung-Ki area were discussed.

불임유우에 있어서 성 Steroid Hormone 수준과 내분비선의 변화에 관한 연구 1 . 저수태우 ( 低受胎牛 )와 무발정란소낭종 ( 無發情卵巢囊腫 ) 유우에 있어서 성 Steroid Hormone 수준의 특성과 Prostaglandin F2α유연물질의 투여효과

정영채,김창근,정영호 ( Y . C . Chung,C . K . Kim,Y . H . Chung ) 한국축산학회 1982 한국축산학회지 Vol.24 No.3

These experiments were carried out to characterize the levels of progesterone and estradiol in serum and milk of repeat breeder cows and to determine the effects of prostaglandin F₂α analogue administration on ovarian function, conception and progesterone levels in anestrous dairy cows. The progesterone levels in serum and milk and estradiol levesl in serum from 2 normal cows 4 repeat breeders were measured at follicular and luteal stage by radioimmunoassay. Eight anestrous cows with cystic corpus luteum or luteal cyst were given 500㎍/2㎖ of PGF₂α analogue followed by 2,000 IU of HCG 60 hr. later to induce oestrus and they were investigated for estrous sign, conception (60-day NR) and sequential progesterone levels after PGF₂α analogue administration. The average serum and milk progesterone level in repeat breeders was 0.38 and 3.02 ng/㎖ at day-1 to +1 (day 0=day of estrus), 1.19 and 6.94 ng/㎖ at day +4 to +9, 5.94 and 9.23 ng/㎖ at day +10 to +15, and 0.88 and 5.41 ng/㎖ at day +17 to +20 respectively. These levels were not significantly different from those of normal cows The average serum estradiol-17β level during follicular stage was 6.67 pg/㎖ at day-4 to -3, 17.19 pg/㎖ at day-2 to-1, 11.55 pg/㎖ at day 0 to +1 respectively. Extradiol-17β level at day 0 to-1 was higher (P$lt;0.05) than 4.91 pg/㎖ of normal cows. The average serum estradiol-17(3 level during luteal stage was 3.72 pg/㎖ at day +2 to +4 and 8.24pg/㎖ at day +10 to +15, respectively, but not significantly different from those of normal cows. The number of cows showed estrous sign within 7 days after PGF₂α administration was 4 out of 8 anestrous cows, 3 out of 4 cows showed good estrous sign and ovulation, and 2 of 3 cows inseminated at first estrus became pregnant. Average serum progesterone level within 8 days after PGF₂α analogue administration in 4 cows showed estrous sign decreased to 25.3% at day 1, 17.6% at day 2, and 26.2% at day 3 of the intitial level (2.21 ng/㎖) prior to administration, but the level at day 8 increased again to 0.81 ng/㎖. The average milk progesterone level at day 1 after PGF₂α analogue administration decreased to 34.6% of the intitial level (4.27 ng/㎖) prior to administration. and the level at day 8 was 2.62 ng/㎖. In 4 cows showed no estrous sign the serum and mi1k progesterone level at the following day of administration decreased also significantly in 3 cows, but increased in 1 cow. There were no marked histological changes in luteal cells within 3 days following PGF₂α analogue administration.

소의 다두분만에 관한 연구 1 . 소의 조기임신진단 방법에 관한 연구

정영채,김창근 ( Yung Chai Chung,Chang Keun Kim ) 한국축산학회 1978 한국축산학회지 Vol.20 No.4

Objectives of this study were to (1) determine the progesterone and oestradiol levels in peripheral plasma daring the estrous cycle in dairy and Korean native cows by radio-immunoassay and (2) apply it to early diagnosis of pregnancy and to hormonal infertility control. Jugular venous blood plasma of 21 dairy and 8 Korean native cows were sampled 11 times each cow at intervals of 2∼4 days from day 11 proestrus to day 25 postestrus and stored at -20℃ until prepared for assay. 1. The levels of progesterone during the estrous cycle in non-pregnant cows began to decline rapidly 5 days before estrus, remained below 1.2ng/㎖ for 1∼2 days before and after estrus, and increased thereafter from day 5 after estrus to a peak of 5∼6ng/㎖ on day 11 to 15. The levels during the luteal phase(day 2 to 15) in dairy cows were 19% higher than those of Korean native cows. 2. The changes in progesterone level of the pregnant cows were similar patterns to those of non-pregnant cows up to 12 days after estrus, but high levels were maintained until day 25 of pregnancy without differences of levels in two breeds. The progesterone levels from day 17 to 25 of pregnancy were about 4∼5 times higher than those of non-pregnant. 3. In changes of oestradiol, non-pregnant cows showed a sharp peak of 12.3∼13.5pg/㎖ at 1∼3 days before estrus and a small ranges of 3.0∼4.5pg/㎖ during the luteal phase of the cycle. The oestradiol levels in pregnant cows approximated that during the luteal phase of non-pregnant, although a little higher than those of non-pregnant cows after day 14 of pregnancy. 4. The progesterone levels on day 24 to 25 of pregnancy averaged 5.93±2.01ng/㎖ ($gt; 5ng/㎖ in 72.7% of cows) in 11 pregnant cows and 1.23±1.21ng/㎖ ($lt;2.5ng/㎖ in 77.8% of cows) in 9 non-pregnant cows. Fused upon the levels 3.4 (non-pregnancy) and 3.9ng/㎖ (pregnancy) on day 24 to 25 after breeding, the average accuracy of forecasting pregnant and non-pregnant was 89.5%.

소의 다두분만에 관한 연구 2 . 우유의 Hormone 분석법에 의한 소의 조기임신진단에 관한 연구

정영채,김창근 ( Yung Chai Chung,Chang Keun Kim ) 한국축산학회 1980 한국축산학회지 Vol.22 No.1

This experiment was carried out to determine the progesterone and oestradiol level in milk during the estrous cycle of dairy cows by radioimmunoassay and to apply it to early pregnancy diagnosis and control of infertility. Whole milk samples were collected at afternoon milking from 61 cows in the Korea-Germany Dairy Farm, National Agricultural Cooperative Federation in An-Seong. Nine cows were sampled 5 times at intervals of 5days during a single estrous cycle to establish the pattern of progesterone and oestradiol levels and 52 cows were sampled twice on day of estrus and day 23 to 26 after artificial insemination to diagnose the pregnancy and infertility. 1. The progesterone levels in milk during a single estrous cycle were the highest on day 15 with a mean of 3.2±0.4 ng/㎖ and were the lowest on day 0 and next estrus. The levels of progesterone on day 5 and day 10 Here found especially to be variable among individuals. There were no significant differences in progesterone levels between the length of estrous cycle. The oestradiol levels in milk were raised at the estrus with a mean of 34.5 to 37.0pg/㎖ and the levels of oestradiol during the luteal phase (day 5 to day 15) were not variable at level of 17.5 to 19.2pg/㎖. 2. The progesterone level, 4.2±0.5ng/㎖, of pregnant cows on day 23 to 26 after insemination was significantly higher than 1.6±0.9ng/㎖ of non-pregnant cows on same days. These levels in milk were significantly higher in pregnant cows that showed the first estrus within 51 to 80 days after calving and conceived within 60 to 90 days after calving and although there were no significant differences, the levels progesterone in milk were also higher in pregnant cows that showed the conception at the 1st insemination and was within the 3rd calving.