두릅 에탄올 추출물의 Chlorogenic acid 함량 분석 및 생리활성

이정호,정경옥,임소연,진다몬,이왕로,Lee, Jeong Ho,Jeong, Kyoung Ok,Im, So Yeon,Jin, Da Mon,Lee, Wang Ro 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5

This study was conducted to quantify chlorogenic acid content and evaluate biological activity, such as antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity of Aralia elata ethanol extract (AEE). The SC₅₀ of DPPH and ABTS radical scavenging activities of AEE were 4.79±0.05 mg/mL, 5.79±0.05 mg/mL; total polyphenol and total flavonoid contents were 170.0±1.8 mgGAE/g, 105.5±4.1 mgQE/g, respectively. Nitric oxide (NO) was increased in RAW 264.7 cells and Caco-2 cells with treatment of LPS, and production of NO was inhibited by AEE in a concentration-dependent manner. Production of NO was reduced by 60.0±1.1% in RAW 264.7 cells and 50.7±2.8% in Caco-2 cells at of AEE. Similarly, the production of inflammatory cytokines (TNF-α, IL-1β and IL-6) was inhibited in a concentration dependent manner. Antibacterial activity increased as the dose concentration of AEE increased, and the MIC was 75 mg/mL for L. monocytogenes, and 100 mg/mL for S. typhimurium and H. pylori. In addition, amylase and protease enzyme activity was observed in AEE and increased enzyme activity was observed according to the concentration of the extract. AEE contained 7.06±0.01 mg/g of chlorogenic acid. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using Aralia elata.

복분자 추출물의 항산화 활성 및 지표성분 표준화를 위한 ellagic acid 검증

이정호(Jeong Ho Lee),정경옥(Kyoung Ok Jeong),임소연(So Yeon Im),이승제(Seung Je Lee) 한국식품저장유통학회 2021 한국식품저장유통학회지 Vol.28 No.5

대두 추출물의 Daidzin 및 Glycitin의 동시분석

임소연 ( So Yeon Im ),정경옥 ( Kyoung Ok Jeong ),이정호 ( Jeong Ho Lee ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.1

대두 추출물의 daidzin과 glycitin을 HPLC를 이용하여 동시분석하였다. HPLC 분석용매는 water/MeOH/acetic acid (88:10:2)와 MeOH/acetic acid (98:2)를 사용하여 C18 MGII컬럼 (4.6 × 250 mm, 5 μm), 유속 1.0 mL/min, 시료 10 μL를 주입하여 컬럼온도 40℃, PDA detector 검출파장 260 nm에서 검출 한계, 정량 한계, 정밀도, 정확성 및 회수율을 측정하였다. Daidzin과 glycitin 표준용액과 대두 추출물은 다른 물질의 간섭 없이 분리되었으며, 검출시간 및 피크 유지시간과 일치하는 스펙트럼이 나타났다. 검량선의 상관계수(R²)는 0.9999의 직선성이 나타났다. Daidzin의 검출한계는 0.1211 μg/mL, 정량한계는 0.3670 μg/mL, glycitin의 검출한계는 0.0493 μg/mL, 정량한계는 0.1494 μg/mL이었으며, 회수율은 daidzin는 97.36-100.29%, glycitin은 83.67-85.39%이었다. 일간 정밀성은 daidzin에서 1.3120 ± 0.0199 - 1.3348 ± 0.0070 mg/g (RSD 0.5214 - 2.2882%), glycitin은 0.4816 ± 0.0026 -0.4895 ± 0.0111 mg/g (RSD 0.5472 - 2.2752%)으로 분석되었다. 실험실내 정밀성은 daidzin에서 1.33762 ± 0.0079 mg/g (RSD 0.5923%), glycitin은 0.4913 ± 0.0101 mg/g (RSD는2.0549%)으로 나타났다. 대두를 추출온도 및 추출시간을 달리하여 추출한 추출물의 daidzin, glycitin의 함량을 측정한 결과 90℃, 3시간 추출물에서 daidzin의 함량이 0.70 ± 0.01 mg/g, glycitin의 함량이 0.25 ± 0.01 mg/g으로 분석되었으며, 추출 온도 및 추출 시간이 증가함에 daidzin과 glycitin의 함량도 증가하였다. 본 분석방법은 HPLC를 이용하여 대두 추출물의 daidzin과 glycitin를 동시에 분석할 수 있는 기초자료라 판단된다. Simultaneous analysis of daidzin and glycitin, the marker compound of soybean extract, was performed using HPLC. HPLC was analyzed with gradient elution of water/MeOH/acetic acid (88:10:2) and MeOH/acetic acid (98:2) and flow rate 1.0 mL/min, C18 MGII column (4.6 × 250 mm, 5 μm), column temperature 40oC, PDA detector 260 nm. Daidzin and glycitin was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, accuracy and recovery by HPLC relative to soybean extract. Daidzin, glycitin standard solution and soybean extract were separated without interference from other substances, and spectrum consistent with the peak retention time appeared. It showed a high linearity in calibration curve with coefficient of correlation (R2) 0.9999. The LOD of daidzin and glycitin were 0.1211 μg/mL, 0.0493 μg/mL, LOQ of daidzin and glycitin were 0.3670 μg/mL, 0.1494 μg/mL, respectively. Inter-day precision of daidzin and glycitin were 1.3120±0.0199- 1.3348±0.0070 mg/g (RSD 0.5214-2.2882%) and 0.4816±0.0026-0.4895±0.0111 mg/g (RSD 0.5472-2.2752%). Intermediate precision of daidzin and glycitin were 1.33762±0.0079 mg/g (RSD 0.5923%) and 0.4913±0.0101 mg/g (RSD 2.0549%). Daidzin 0.70±0.01 mg/g and glycitin 0.25±0.01 mg/g were analyzed in soybean extract extracted at 90oC for 3 h. As the extraction temperature and extraction time were increased, the contents of daidzin and glycitin also increased. This result confirmed that simultaneous analysis of daidzin and glycitin of soybean extract was possible using HPLC.

산수유 추출조건에 따른 Loganin 함량 분석 및 활성 평가

임소연 ( So Yeon Im ),정경옥 ( Kyoung Ok Jeong ),이정호 ( Jeong Ho Lee ) 한국키틴키토산학회 2020 한국키틴키토산학회지 Vol.25 No.3

산수유는 인체 건강에 유익하여 전북지역에서 재배가 증가하고 있어 기능성식품을 개발하고자 loganin의 함량과 항산화, 항당뇨 및 항염 활성, 일반성분을 분석하였다. 산수유에 함유된 loganin 함량을 분석한 결과 3시간, 100℃ 추출물에서 7.68 ± 0.06 mg/g, 9시간 100℃ 추출물에서 7.88 ± 0.04 mg/g 분석되었으며, 추출시간 및 추출온도가 증가함에 따라 loganin 함량도 증가하였다. 산수유 100oC 3시간 추출물 1,000 μg/mL에서 DPPH 라디칼 소거활성은 53.58 ± 3.35%, ABTS 라디칼 소거활성은 18.22 ± 0.62%, SOD 효소 활성은 69.6 ± 7.42%으로 측정되었으며, 추출물의 처리농도가 증가함에 따라 항산화 활성도 증가하였다. 세포 독성은 나타나지 않았으며, NO 생성 저해 활성은 1 μg/mL에서 12.88 ± 0.24, 10 μg/mL 12.89 ± 0.96으로 저해되었다. α-Glucosidase 저해 활성은 1,000 μg/mL에서 98.9 ± 5.95%, 500 μg/mL에서 91.9 ± 5.28%으로 분석되었다. 일반 성분은 열량 344.94 kcal/100 g, 탄수화물 80.83% 조단백질 3.29%, 조지방 0.94%, 총당 347.61 mg/g으로 분석되었다. 산수유에는 loganin이 함유되어 있고 항산화, 항암, 항당뇨 활성이 있어 기능성 소재로 개발이 가능할 것으로 판단된다. The aim of this study was to investigate the potential of Cornus officinals as a content of protocatechuic acid and physiological activities. The analyzed the content of protocatechuic acid of Cornus officinals water extracts according to different extract temperature and time for extraction. It was analyzed as 7.68 ± 0.06 mg/g in the extract at 100℃ for 3 h. Protocatechuic acid content increased as extraction time and temperature increased. The activity of the Cornus officinals water extract at 100℃ for 3 h was measured. Antioxidant activity against the concentration of Cornus officinals water extract 1,000 μg/mL was measured. DPPH radical scavenging activity was measured at 53.58 ± 3.35%, ABTS radical scavenging activity at 18.22 ± 0.62%, and SOD activity at 69.6 ± 7.42%, antioxidant activity also increased as the processing concentration of extracts increased. Cell viability was shown for RAW 264.7 cells, and nitric oxide(NO) production was also inhibited. The α-Glucosidase inhibitory activity was determined to be 98.9 ± 4.45% at concentration of 1000 μg/mL. The nutritional components were analyzed to be 344.94 kcal/100g of calories, 80.83% of carbohydrates, 3.29% of crude protein, 0.94% of crude fat, and 347.61 mg/g of total sugars. Cornus officinals is thought to be developed as a functional food material because it contains protocatechuic acid and has antioxidant activity, anti-inflammatory and antidiabetic activity.

감국(Chrysanthemum indicum L.) 추출물과 분획물의 항산화 및 α-Glucosidase와 PTP1B 저해 활성

황성우,이훈연,차지윤,김보람,박민,이보리,문광현,이정호,김대근,김욱,정경옥,이영미,Hwang, Sung-Woo,Cha, Ji-Yun,Lee, Hoon-Yeon,Kim, Bo-Ram,Park, Min,Lee, Bo-Ri,Moon, Kwang- Hyun,Lee, Jeong-Ho,Kim, Dae-Geun,Kim, Wook,Jeong, Kyoung-Ok,Lee, 동아시아식생활학회 2017 동아시아식생활학회지 Vol.27 No.2

본 연구에서는 국화과에 속하는 Chrysanthemum indicum L.(CI)의 70% EtOH 추출물 및 분획물(dichloromethane, ethyl acetate 및 H2O)의 폴리페놀 함량, 플라보노이드 함량, 항산화 활성 및 생리활성을 평가하였다. 항산화 활성으로는 DPPH, ABTS 및 superoxide 소거 활성을 확인하였고, 생리활성 평가는 ${\alpha}$-glucosidase와 PTP1B 저해 활성을 측정하였다. 그 결과, CI 70% EtOH 추출물에서 폴리페놀과 플라보노이드의 함량은 각각 30.20 mg 및 31.58 mg인 것으로 확인되었다. 여러 분획물 중 EA 분획물이 폴리페놀(87.87 mg)과 플라보노이드(65.12 mg)의 함량이 가장 높았다. EA 분획물의 DPPH, ABTS 및 SOD 소거능은 각각 93.84%, 93.38% 및 84.68%의 억제를 나타냈다. EA 분획물은 70% EtOH 추출물 및 다른 분획과 비교하여 가장 높은 항산화 활성을 보였다. 또한 EA 분획물($20{\mu}g/mL$)의 ${\alpha}$-glucosidase 및 PTP1B 억제 결과는 다른 시료에 비해 각각 30.29% 및 99.24%의 가장 높은 억제를 나타내었다. 본 연구 결과를 통해 감국의 EA 분획물이 70% EtOH 추출물 및 다른 분획물보다 많은 항산화 및 항당뇨 성분을 함유하는 것으로 보여진다. 결론적으로 우리는 CI의 천연 항산화 물질 및 기능성 물질에 성능이 좋아 화장품 첨가제나 음료로도 개발이 가능할 수 있을 것으로 사료된다. In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of ${\alpha}$-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction ($20{\mu}g/mL$) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.

감국(Chrysanthemum indicum L.) 추출물과 분획물의 항산화 및 α-Glucosidase와 PTP1B 저해 활성

황성우(Sung-Woo Hwang),이훈연(Hoon-Yeon Lee),차지윤(Ji-Yun Cha),김보람(Bo-Ram Kim),박민(Min Park),이보리(Bo-Ri Lee),문광현(Kwang- Hyun Moon),이정호(Jeong-Ho Lee),김대근(Dae-Geun Kim),김욱(Wook Kim),정경옥(Kyoung-Ok Jeong),이영미(Young 동아시아식생활학회 2017 동아시아식생활학회지 Vol.27 No.2