확률모형을 이용한 오제스키병 혈청학적 모니터링 프로그램 평가

장기윤,박선일,박최규,이경기,주이석,Chang, Ki-Yoon,Pak, Son-Il,Park, Choi-Kyu,Lee, Kyoung-Ki,Joo, Yi-Seok 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.2

The objective of this study was to analyze data from the planned national serological monitoring program for Aujeszky's disease (AD) using a simulation model to evaluate probable outcomes expected in the sample derived from the simulated herds at predefined within-herd prevalence and herd prevalence. Additionally, prevalence at animal- and herd-level estimated by the stochastic simulation model based on the distributions of the proportion of infected herds and test-positive animals was compared with those of data from a national serological survey in 2006, in which 106,762 fattening pigs from 5,325 herds were tested for AD using a commercial ELISA kit. A fixed value of 95% was used for test sensitivity, and the specificity was modeled with a minimum, most likely and maximum of 95, 97 and 99%, respectively. The within-herd prevalence and herd prevalence was modeled using Pert and Triang distributions, respectively with a minimum, most likely and maximum point values. In all calculations, population size of 1,000 was used due to lack of representative information. The mean number of infected herds and true test-positives was estimated to be 27 herds (median = 25; 95% percentile 44) and 214 pigs (median = 196; 95% percentile 423), respectively. When testing 20 pigs (mean of 2006 survey) in each herd, there was a 3.3% probability that the potential for false-positive reactions due to less than 100% specificity of the ELISA test would be detected. It was found that the model showed prevalence of 0.21% (99% percentile 0.50%) and 0.5% (99% percentile 0.99%) at animal- and herd-level, respectively. These rates were much similar to data from the 2006 survey (0.62% versus 0.83%). The overall mean herd-level sensitivity of the 2006 survey for fattening pigs was 99.9%, with only a 0.2% probability of failing to detect at least one infected herd.

돼지에 殘留된 설파劑의 微生物學的 스크리닝法에 關한 硏究

Ki Yoon CHANG(張基允),Soon Jae KIM(金順在),Jong Myung PARK(朴鍾鳴) 한국예방수의학회 1991 예방수의학회지 Vol.15 No.1

A simplified procedure was described for screening swine urine for the presence of sulfonamide residues. The principle of method is synergism and antagonism of sulfonamide. When sulfonamide is combined with trimetoprim(TMP), antibacterial synergism is obtained. On the other hand, when excessp-aminobenzoic acid(PABA) is present the antibacterial activity of the sulfonamides is overcome. The method involved soaking two discs into swine urine. Each disc is placed on couple of test plates using EEC-four plate inoculated with Bacilus subtilis spore at pH7.2. One plate is incorporated with TMP(TMP-plate), another is PABA(PABA-plate). Diffusion of an antibacterial substance is shown by the formation of zones of inhibition(inhibition zone). Particularly inhibition zone on TMP-plate than PABA-plate considers good evidence to send the specimen for specific sulfonamide residues test. Of a total 220 urine samples tested by this screening method, 63 cases were showed the inhibition zones. Among them, inhibition zones were only shown on TMP-plete in 12 cases and inhibition zones on TMP-plate were much greater than PABA-plate in 8 cases. Accompanied with the microbioassay, thin-layer chromatographic(TLC) tests for screening the sulfonamide residues with Sulfa-On-site(SOS) kit were applied to same samples in which two positive cases were shown. The sulfamethazine residues in 2 TLC positive urine were identified by high performance liquid chromatographic(HPLC) test. One TLC positive sample is one of 12 cases in which inhibition zones on TMP-plate were only shown and another TLC positive sample was also included in much greater inhibition zones on TMP-plate cases. With this result, the possibility of screening the sulfonamide residues in swine urine by this cases. With this result, the possibility of screening the sulfonamide residues in swine urine by this microbioassay is obtained.

돼지 지방 조직 및 골수 유래 성체줄기세포의 성상분석과 다능성에 관한 연구

이아영,최경임,나진주,소병재,이경우,장기윤,송재영,차상호,Lee, Ah-Young,Choe, Gyeong-Im,Nah, Jin-Ju,So, ByungJae,Lee, Kyung-Woo,Chang, Ki-Yoon,Song, Jae-Young,Cha, Sang-Ho 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.

원유중의 잔류항생물질 및 썰파제 조사

박병옥 ( Byoung Ok Park ),백미순 ( Mi Soon Back ),권기호 ( Ki Ho Kwon ),우기방 ( Ki Bang Woo ),장기윤 ( Ki Yoon Chang ) 한국가축위생학회 1991 韓國家畜衛生學會誌 Vol.14 No.1

Antibiotic residues of raw milks collected in Anyang area were tested by TTC-reduction test, EEC-4plate method and TLC(SOS-kit) method to improve and monitor the quality of milk. Antibiotic substances were not detected from 100 raw milk samples, but sulfamethazine was detected from only one sample(1.3PPM) by SOS test. Unclassified anti-microbic substances were detected from 22 samples by EEC-4 plate method. EEC-4 plate test was useful to categorize the species of antibacterial substances and SOS test was useful to detect the sulfamethazine field screening test.

돼지에 殘留된 설파劑의 微生物學的 스크리닝法에 關한 硏究

張基允,金順在,朴鍾鳴 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.1

돼지에 잔류된 설파제의 미생물학적 스크리닝법에 관한 연구

장기윤,김순재 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

A simplified procedure is described for screening swine urine for the presence of sulfonamides residues. The principle of method is synergism and antagonism of sulfonamide. When sulfonamide is combined with trimetoprim(TMP), antibacterial synergism is obtained. On the other hand, when excess p-aminobenzoic acid(PABA) is present the antibacterial activity of the sulfonamides is overcome. THe method involved soaking two discs into pork urine. Each disc is placed on couple of test plates using EEC-four plate inoculated with Bacilus subtilis spore at pH 7.2. One plate is incorporated with TMP(TMP-plate), another is PABA(PABA-plate). Diffusion of an antibacterial substance is shown by the formation of ones of inhibition(inhibition zone). Particularly either the inhibition zone on TMP-plate only or much greater inhibition zone on TMP-plate than PABA-plate considers good evidence to send the specimen for specific sulfonamide residues test. Of a total 220 urine samples tested by this screening method, 63 cases were showed the inhibition zones. Among them inhibition zones were only shown on TMP-plate in 12 cases and inhibition zones on TMP-plate were much greater than PABA-plate in 8 cases. Accompanied with the microbioassay, thin-layer chromatographic(TLC) tests for screening the sulfonamide residues with Sulfa-On-Site(SOS) kit were applied to same samples in which two positive cases were shown . The sulfamethazine residues in 2 TLC positive urine were identified by high performance liquid chromatographic(HPLC) test. One TLC positive urine is one of 12 cases in which inhibition zones on TMP-plate were only shown and another TLC positive urine was also included in much greater inhibition zones on TMP-plate cases. With this result, the possibility of screening the sulfonamide residues in swine urine by this microbioassay is obtained.