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가루깍지벌레(Pseudococcus comstocki (Kuwana))월동알의 온도발육 및 부화시기예찰모형
전흥용,김동순,임명순,이준호,Jeon, Heung-Yong,Kim, Dong-Soon,Yiem, Myoung-Soon,Lee, Joon-Ho 한국응용곤충학회 1996 한국응용곤충학회지 Vol.35 No.2
가루깍지벌레 월동안 부화시기 예찰모형을 수립하기 위하여 월동알에 대한 온도발육 실험이 수행된다. 5개온도(10, 15, 20, 25, 27$^{\circ}C$)와 채집시기별로 월동알 부화기간을조사하여 비교분석하였다. Wagner 등 (1984a)의 비선형발육모형이 온도별 평균발육률에 대하여 적용되었으며 ($R^2$=0.9729). 발육영점온도는 15~$25^{\circ}C$ 영역에서 얻은 직선회귀식에 의하여 $11.9^{\circ}C$로 추정되었으며, 발육완료를 위해서는 154.14일도가 필요하였다. 적산온도 모형과 발육률전산(Wagner 등 1985) 모형이 부화시기 예측에 이용된다. 적산온도 모형은 평균온도에서 발육영점온도 이상의 온도를 적산하는 방법(Mean-minus-base 추정법), Sine wave 추정법(Allen 1976), 그리고 Rectangle 추정법(Arnold 1960)을 잉요하여 계산하였다. 50% 부화예측일을 실측일과 비교한 결과 적산온도를 이용하는 경우 Mean-minus-base 추정법은 18~28일, Sine wave 추정법은 11~14일, 그리고 Rectangle 추정법은 3~5일의 편차를 보였고, 발육률 적산모형은 2~3일의 편차가 있었다. Temperature-dependent development study for overwintered eggs of Pseudococcus comstocki (Kuwana) wasconducted to develop a forecasting model for egg hatch date. Hatch times of overwintered eggs were comparedat five constant temperatures (10, 15, 20, 25, 27$^{\circ}$C) and different collection dates. A nonlinear, four-parameterdevelopmental model with high temperature inhibition accurately described (R2=0.9948) mean developmentalrates of all temperatures. Variation in developmental times was modeled(~~=0.972w9)it h a cumulative Weibullfunction. Least-squares linear regression (rate=O.O06358[Temp.]-0.07566)d escribed development in the linearregion (15-25$^{\circ}$C) of the development curve. The low development threshold temperature was estimated 11.9"Cand 154.14 degree-days were required for complete development. The linear degree-day model (thermal summation)and rate summation model (Wagner et al. 1985) were validated using field phenology data. In degreedaymodels, mean-minus-base method, sine wave method, and rectangle method were used in estimation of dailythermal units. Mean-minus-base method was 18 to 28d late, sine wave method was 11 to 14d late, rectanglemethod was 3 to 5d late, and rate summation model was 2 to 3d late in predicting 50% hatch of overwinteredeggs. hatch of overwintered eggs.
열처리와 생장점 배양 및 항바이러스제 처리에 의한 포도 GLRaV-3의 무독화효과
김현란,정재동,박진우,최용문,임명순,Kim, Hyun-Ran,Chung, Jae-Dong,Park, Jin-Woo,Choi, Yong-Mun,Yiem, Myoung-Soon 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
Grapevine leafroll-associated virus 3(GLRaV-3) is one of the most severe pathogens for viral diseases found in Korea. This study was conducted to establish the virus-free stock production system for the virus disease control. The effects of thermotherapy, merestem culture and chemotheratpy to eliminate the GLRaV-3 in gratevines were tested. Thermotherapy at 37$\pm$2$^{\circ}C$ for 6∼8 weeks combined with 0.5∼1.0mm size of meristem culture method was the most effective for virus elimination. Thermotherapy alone was not effective. In chemotheratpy, DHT and Amantadine (20, 40mg/L) treatment in medium was more effective than Ribavirin to eliminate the GLRaV-3 in grapevine. However, Ribavirin spraying to potted was not available for virus elimination. Therefore, virus-free stock production system using the thermotherapy combined with shoot apical meristem culture was the most effective in grapevine.