β-Carotene Hydroxylase 관련 Chyb 유전자를 이용한 형질전환 Arabidopsis에서 Astaxanthin의 생합성

이호재,강권규,Lee, Ho-Jae,Kang, Kwon-Kyoo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

Oxycarotenoids는 녹색식물, 곰팡이, 효모, 버섯 및 세균 등이 만들어 내는 황색, 적색 또는 자색의 polyene계 색소로 분자내에 산소를 함유하며 생체내에서 중요한 역할을 담당하고 있다. 본 실험에서는 Oxycarotenoids의 생합성 경로상에 존재하는 $\beta$-carotene hydroxylase 유전자 (Chyb)가 재조합된 Ti-plasmid (pGCHYB)를 A. tumerfacience GV3101에 의해 Arabidopsis thaliana (cv. Columbia)에 형질전환하였다. 50 mg/L hygromycin 함유한 MS 배지에서 선발된 개체를 이용하여 Chyb 유전자의 도입여부를 PCR로 분석한 결과, 대조구에서는 Chyb 유전자의 증폭 되지 않았으나 형질전환체에서는 증폭 산물을 확인 할 수 있었다. 또한 형질전환체의 발현여부를 RT-PCR분석한 결과 도입된 Chyb 유전자가 안정적으로 발현되었다. 형질전환체의 carotenoids를 HPLC 분석한 결과 xanthophyll cycle carotenoids (violaxanthin과 zeaxanthin)의 함량 및 $\beta$-carotene 함량은 감소되었으며, 대조구 Arabidopsis에는 생합성되지 않는 astaxanthin이 생합성되었다. 따라서 본 실험에서 육성된 형질전환체를 이용하여 oxycarotenoids 생합성 과정상의 중간대사물질의 표지, 관여된 transcript 및 metabolite 분석 등을 통해 carotenoids 대사계의 연구소재로 활용 할 수 있을 것으로 기대한다. Oxycarotenoids are oxygenated carotenoids that perform critical roles in plants. $\beta$-Carotene hydroxylase adds hydroxyl groups to the $\beta$-rings of carotenes and has been cloned from several bacteria and plants including Arabidopsis. This study was carried out to investigate the effect of $\beta$-carotene hydroxylase gene (Chyb) on the oxycarotenoids biosynthesis in the transgenic Arabidopsis. Construct of pGCHYB containing Chyb was established onto Gateway vector system (pENTR3C gateway vector and pH2GW7 destination vector). Arabidopsis thaliana (cv. Columbia) was transformed with Agrobacterium tumerfacience GV3101 harboring pGCHYB construct driven by 35S promoter and hygromycin resistant gene. Seven hundred bases paired PCR products, indicating the presence of Chyb gene, were found in the transformants by PCR analysis using Chyb primers. Hygromycin resistance assay showed that transgenes were stably inherited to next generation. The overexpression of the Chyb gene resulted in the decrease carotenoid content. Especially, astaxanthin unusual oxycarotenoid in wild type Arabidopsis was detected in the transgenic plants. This means that decreased carotenoids might be converted into astaxanthin metabolism with the aid of silent gene in the host.

SDH기본 시스팀의 구현 및 망구성 전략

이호재,김홍주,김재근,Lee, Ho-Jae,Kim, Hong-Ju,Kim, Jae-Geun 한국전자통신연구원 1992 전자통신 Vol.14 No.2

본고에서는 CCITT 표준에 기본을 둔 다양한 전송서비스 능력을 갖는 SDH 망요소들의 모듈화 구현기법을 제시하고, 나아가 이들 망요소들을 응용한 동기식 전송망의 구조를 전송서비스의 보호특성을 강조하여 제시한다.

《금궤요략·부인임신병맥증치(婦人姙娠病脈證治)》편(篇)에 대(對)한 문헌적(文獻的) 고찰(考察)

이호재,김동희,류동열,Lee, Ho-Jae,Kim, Dong-Hui,Ryu, Dong-Yeol 대전대학교 한의학연구소 1998 한의학연구소 논문집 Vol.7 No.1

컴퓨터 맥진시스템

이호재,허웅,Lee, Ho-Jae,Huh, Woong 대한전자공학회 1996 전자공학회논문지-B Vol.b33 No.4

In this paper, we implemented a computerized pulse diagnosis system for the developement of pulse diagnosis algorithms and the generalization of the pulse diagnosis. The system consists of the hardware and software. The hardware detects pulse waves and inputs the waves into the computer system, while the software not only manages and analyzes the input pulse wave data but also privides the database. In order to clinically test the developed pulse diagnosis system, we applied the carotid-radial pulse diagnosis algorithm to the system. As the results, it is found that the genralizatio of the pulse diagnosis is possible.

테트라싸이클린 처리된 흡수성 및 비흡수성 조직유도재생술용 막에의 세균부착과 침투양상

이호재,정현주,Lee, Ho-Jae,Chung, Hyun-Ju 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1

The barrier membranes for GTR procedure could be affected bY bacterial contamination after exposure to oral environment. This study was done to evaluate whether the tetracycline impregnated barrier membranes could inhibit bacterial attachment and penetration into membranes. The resorbable membrane(polylactic and polyglycolide copolymer, $Resolute^{(R)}$, W.L Gore and Associates, Inc..USA) and the non-resorbable membrane(e-PTFE; Gore-TexTM, W.L. Gore & Associates, Inc.,USA) were cut into 4mm discs and trated with 5% tridodecylmethylammonium chloride solution in ethanol and dried in air. The membranes were immersed in tetracycline(TC) solution (100mg/ml, pH 8.0) and dried. To the maxillary canine-premolar region in six periodontally healthy volunteers, removable acrylic devices were inserted, on which 8 cylindrical chambers were glued with TC impregnated and non-impregnated discs, the membrane discs were examined for bacterial attachment and penetration, and structural changes under SEM and LM. From the 1st day to the 7th day, membranes showed bacterial plaque formation composed of cocci and rods. Thereafter, filamentous bacteria appeared and the plaque thickness increased. The TC impregnated e-PTFE membranes showed less bacterial attachment and delayed in bacterial plaque maturation than non-treated membranes. As for bacterial penetration, the TC impregnated e-PTFE membranes showed superficial invasion and infrequent presence of bacteria in unexposed inner surface at the 4th week. while the non-treated e-PTFE membranes showed deep bacterial invasion at the 2nd week and frequent presence of internal bacteria at the 4th week. The resorbable membranes started to be resorbed at the 2nd week and were perforated at the 4th week, regardless of TC treatment. In conclusion, bacterial plaque formation and penetration was efficiently delayed in TC impregnated e-PTFE membranes, whereas resorbable membranes were similar in bacterial invasion due to membrane degradation and perforation, regardless of TC treatment.

백서 치주인대세포의 분화에 대한 Bone morphogenetic protein-7의 영향

이호재,김영준,정현주,Lee, Ho-Jae,Kim, Young-Jun,Chung, Hyun-Ju 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.3

Periodontal therapy has dealt primarily with attempts at arresting progression of disease. however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. Recombinant human bone morphogenetic protein-7(rhBMP-7) can differentiate the osteoprogenitor cells and induce bone formation. The purpose of this study was to evaluate the effect of BMP-7 on rat periodontal ligament cells differentiation, in vitro. In the control group, cells was cultured with DMEM media. In the experimental groups, cells were cultured with rhBMP-7 in concentration of 10, 25, 50 and 100 ng/ml. Each group was characterized by examining alkaline phosphatase activity at 3 and 5 days of culture and the ability to produce mineralized nodules of rat calvarial cells at 14 days of culture. Synthesis of type I collagen(COL-I), osteocalcin(OCN), and bone sialoprotein(BSP) was evaluated by RT-PCR at 7 days of culture. Activation of Smad proteins and p38 MAP kinase was determined by western blot analysis of the cell lysates. Alkaline phosphatase activity was significantly increased in the concentration of BMP-7 50 ng/ml and 100 ng/ml compared to the control(p<0.05). The mineralized bone nodule formation was greater with addition of 50 ng/ml and 100 ng/ml BMP-7 than the control(p<0.01). In 7 days' culture, the expressions of COL-I, BSP, and OCN was increased by BMP-7 in concentration of 10 $ng/ml{\sim}100$ ng/ml. In western blot analysis, BMP-7 treated culture cells expressed Smad 1,5,8 in dose-dependent manner, whereas BMP-7 did not activate phosphorylated form of p38 MAP kinase. These result suggested that BMP-7 stimulate rat periodontal ligament cells to differentiate toward osteoblast phenotype and increase bone matrix production by activation of BMP-Smad pathway.

인영.촌구 대비법을 이용한 맥 진단 시스템 구현

이호재,박영배,허웅,Lee, Ho-Jae,Park, Young-Bae,Huh, Woong 대한의용생체공학회 1993 의공학회지 Vol.14 No.1

This paper describes the implementation of a computerized radial pulse diagnosis by Elds of a clinical expert. On this base, we composed of the radial pulse diagnosis system in korean traditional medicine. The system composed of a radial pulse wave detection system and a radial pulse diag nosis system. With a detection system, we detected Inyoung and Cheongu radial pulse wave and processed It. Then, we have got the characteristic parameters of radial pulse wave and also quantified thats according to the method of Inyoung-Cheongu Comparison Radial Pulse Diagnosis. We defined the jugement standard of radial Pulse diagnosis system and then we confirmed the PossibiliLy for realization of automatic radial Pulse diagnosis in korean traditional medicine.

기획논문: 신종교와 성소(1) : 중국 민간종교 한조묘의 종교문화적 함의

이호재 ( Ho Jae Lee ) 한국신종교학회 2015 신종교연구 Vol.33 No.-

이 글은 현대 중국의 종교정책의 변화에 따른 홍양교의 종교건축 구조에 나타난 종교 문화적 의미를 규명하는 데 있다. 현대 중국은 역대 왕조와 같이 종교 성소관리를 통해 민중의 종교를 체제안에서 관리하려는 시험을 하고 있는 현장의 역사적 맥락을 고찰해 보는 것과 동시에 명대 중기의 홍양교 성지인 한조묘의 복원과정에 나타난 종교성소의 실증적인 분석을 통하여 종교성소의 관리가 통치계급이 사회적 질서를 안정시키는데 유효한 수단으로 활용하고 있음을 확인해 볼 것이다. 이를 위해 한조묘의 종교 건축물의 구조 분석을 통해 한조궁의 벽화와 신상의 변화는 물론 각 개별 성전의 상세한 서술을 통해 종교건축물이 지닌 종교 문화적 의미를 밝혔다, 지방정부의 입장에서는 도관(道觀)의 모양새를 외형적으로나마 갖춘 형태이고, 향촌인의 입장에서는 민속신앙의 신상들이 한조묘에 수용된 종교적 질서구조로 이해할 수 있으며, 홍양교 관계자의 관점에서는 홍양교의 주요 신격을 한조묘의 중심에 두어 자신의 정체성을 상실하지 않는 절묘한 공존의 종교성소를 관찰할수 있다. 한편 한조묘는 1992년부터 원형복원에 치중한 재건 양상을 보이는 한편, 종교성소의 명칭을 변경하여, 신상의 형상을 변경하는 등의 중국 당국의 종교정책을 수용하여 변화된 종교적 환경에 적응해 가는 형태를 보이면서도, 민간종교의 정체성을 보존하고 계승하는 모습을 조명하였다. 특히 2008년에 공식적인 종교성소의 허가권이 박탈된 이후에도 정기적인 종교축제를 통하여 단절없는 종교 문화를 재현하지만, 민간위원회는 홍양교의 생존을 위해 도관으로서 면모를 갖추기 위해 삼청전과 전신전, 민속신앙의 용왕전을 신축하며 지방정부의 종교정책에 순응하고 타협하는 현장을 확인할 수 있으며, 이런 과정에서도 홍양교의 정체성을 지키는 민중 종교의 끈질긴 생명력을 확인할 수 있다. 또한 우리는 한조묘가 마스터플랜을 가진 체계적으로 복원되는 종교성소가 아니라, 관주도가 아닌 민간주도의 복원현장이기에 성소의 복원과정이 일관성이 부족하다는 것을 알 수 있지만 이런 통일성의 결여가 오히려 현대 중국의 종교정책 전망을 예측하는 하나의 도구이며, 중국의 전통 종교문화 보존과 계승을 하는 핵심적인 종교 문화요소로서 변화 중인 민간종교의 면모를 고찰할 수 있는 사례임을 이 글은 밝히고 있다. This writing aims at inquiring into religious, cultural meaning shown in religious architectural structure of Vast Yang Teaching (Hongyangjiao, 弘陽敎) consequent on the change of modern China’s religious policy. This writing is intending to consider the historical context of the real spot where modern China is doing atest attempting at control of popular religion under its national framework through Popular Temple management just as the former dynasties did before, and also for this purpose, this writing will confirm that the ruling class is utilizing Popular Temple management as an effective means of stabilizing social order through empirical analysis of a Popular Temple revealed in the restoration process of the Hanzu Temple(Hanzumiao, 韓祖廟) which was the holy place of Vast Yang Teaching in the middle of the Ming Dynasty. For this, this writing traced not only the change of mural and joss of Hanzu Palace(Hanzugong, 韓祖宮) through the structure analysis of religious architecture of Hanzu Temple but also the religious, cultural meaning of religious architecture through detailed description of individual Popular Temple. From the position of the local goverrnment, Hanzu Temple assumes the appearance is equipped with Taoist Temple(Daoguan, 道觀) outwardly though while from the position of the country villagers, it could be understood as the religious order structure, through which the joss of popular religion is accommodated in Hanzu Temple in addition, from the viewpoint of the interested parties of Vast Yang Teaching, Vast Yang Teaching doesn’t lose its identity by placing the major divinity of Vast Yang Teaching in the center of Hanzu Temple, through which it’s possible to observe the religious temple of exquisite coexistence. Meanwhile, Hanzu Temple has shown the reconstruction aspect with focus on restoration to its original state since 1992, and casts light upon the appearance of preserving and inheriting the identity of popular religion while showing the attitude of becoming adapted to the changed religious environment by accepting the Chinese government’s religion policy, such as alteration of the joss form by changing the name of the Popular Temple. Particularly, even after it was stripped of the official religion in 2008, Hanzu Temple reproduces non-interrupted religious culture through regular religious festivals; in addition, it is possible to confirm the real picture of the process, in which the private committee complies & compromises with the local government’s religion policy while constructing the Hall of the Three Pure(Sanqingdian, 三淸殿), the Hall of the All God(Quanshendian, 全神殿) and the Hall of the Dragon King (Longwangdianm 龍王殿) of popular religion in order to be equipped with the appearance as a Taoist Temple for survival of Vast Yang Teaching. Furthermore, it’s also possible to confirm the persistent life force of popular religion which keeps the identity of Vast Yang Teaching even in such process. We are able to perceive that Hanzu Temple is not the Popular Temple which is systematically restored under the master plan but the private-initiated restoration site other than the governmentinitiated one, and therefore the restoration process of the sanctuary lacks consistency; however, this writing is stating its view that such a lack of unity is rather a tool that can predict the outlook for modern China’s religion policy, and that Hanzu Temple is an example that makes it possible for us to consider the appearance of popular religion which is undergoing a change as a core religious culture element which preserves and inherits China’s traditional religious culture.

3D 프린팅 모르타르의 적층간 공극분포 연구

이호재(Lee, Ho Jae),김원우(Kim, Won Woo),서은아(Seo, Eun A),문재흠(Moon, Jae Heum) 한국콘크리트학회 2019 한국콘크리트학회 학술대회 논문집 Vol.31 No.1

운영비용을 고려한 농업용 양수장 계획의 최적화에 관한 연구

이호재 ( Yi Ho Jae ),이정재 ( Lee Jeong Jae ) 한국농공학회 1998 한국농공학회 학술대회초록집 Vol.1998 No.-

An optimization procedure was proposed to minimize construction and operation cost simultaneously. The global criterion method was exploited to find a Pareto optimum solution to the multi-objective functions. The result was compared to the data of a real pumping plant and it was found that the optimized plan of pumping plant could reduce both of the construction and operation cost.