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Provision of efficient online information for agricultural biotechnology
이범규,김종민 충남대학교 농업과학연구소 2020 Korean Journal of Agricultural Science Vol.47 No.2
This study identified consumer perceptions of biotechnology crops, provided the types and sources of information on agricultural biotech that consumers demand, and derived effective methods of providing agricultural biotech information by analyzing problems and improving the information available online regarding agricultural biotech. The analysis of sources of information on agricultural biotech showed that there were differences between preference and reliability. Respondents preferred the Internet (47.6%) and TV (36.3%), while they relied on TV (36.3%) the most, followed by the Internet (26.6%), and academic papers and technical books (23.1%). Only 27.1% of the respondents answered that they collect information on agricultural biotech proactively. The higher frequency of information collection indicated a higher satisfaction rate with the information that was being collected. Survey results for the websites that respondents preferred and relied on to collect information were that the most preferred websites were web portals (53.4%), while reliability rates across the various types of websites were relatively even: web portals (28.4%), academic institution websites (19.1%), and websites that provide professional information (18.2%). Surveys that examined factors that were important in choosing the websites for collecting information on biotech indicated that factors such as “Providing verified data and citation” and “Providing objectivity” were the most important. Examining the preferences and factors of preference by content type showed that the demand for visual aids, such as photos, tables, graphs, and videos, was high, and there were statistically significant differences between the factors of preference by content type.
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이범규,Park Jongsun 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5
Various cultivars of Zoysia japonica Steud. have been developed as genetically modified organisms for commercial purposes. The chloroplast genome was completed to investigate genetic diversity in Z. japonica isolated in Korea. The chloroplast genome of Z. japonica is 135,884 bp long (GC ratio is 38.4%) and has four subregions: 81,378 bp of large single copy (36.3%) and 12,582 bp of small single copy (32.7%) regions are separated by 20,962 bp of inverted repeat (44.1%) regions includ- ing 130 genes (83 protein-coding genes (PCGs), eight rRNAs, 38 tRNAs, and one pseudogene). 68 SNPs and 24 INDEL regions were identified from the two Z. japonica chloroplast genomes, similar to those of Z. matrella. 12 SNPs located in PCGs can be good candidates for developing molecular markers of Z. japonica. Based on nucleotide diversity analysis, atpB/ rbcL, rpl16/rps3, and rpl32/trnL regions exhibited the high nucleotide diversity, which can be used for developing species- specific marker sequences to distinguish their species. Phylogenetic analysis presented that two Z. japonica and Z. sinica were clustered together, indicating that they have been used for developing cultivars of Z. japonica.
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고무로 결합된 과산화수소 감응 탄소반죽 전극의 빠른 감응
이범규,윤길중 한국과학수사학회 2015 과학수사학회지 Vol.9 No.4
과산화수소 정량을 위하여 고추냉이의 과산화효소를 효소원으로, 클로로술폰화 고무를 결합재로 사용하여 효소전극을 만들고 전압전류법으로 전기화학적 특성을 살펴보았다. 계가 보여주는 Lineweaver-Burk 도시의 직선성은 H2O2의 분해가 전극표면에 고정된 HRP에 의하여 촉진되고 있었고, 얻어진 대칭인자(α) 및 교환전류밀도(i0)를 포함한 10개 이상의 전기화학 파라미터는 전극이 정량적으로 작동하고 있음을 확인하여 주었다. 특히 2초보다 짧은 전극신호의 포화시간은 지금까지 소개된 과산화수소 감응 탄소반죽전극 중에서 가장 빠른 감응시간이었다. 이 같은 실험적 결과는 법과학 기법처럼 많은 시료의 신속한 분석을 요구하는 화학분석 분야에 응용될 수 있는 이론적인 기초를 제공할 것이다.
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양이온 교환수지에 대한 희토류 원소의 흡수에 미치는 Coion의 영향
이범규,김인숙,김강진,Beom-Gyu Lee,In-sook Kim,Kang-Jin Kim 대한화학회 1983 대한화학회지 Vol.27 No.1
희토류 양이온이 4M 이상의 염산과 과염소산 용액에서 양이온 교환지수에 예상보다도 큰 흡수를 나타내는 현상을 알아보기 위하여, $1{\sim}12M 염산 또는 염산-과염소산 혼합용액에서 Dowex 50W-X2 양이온 교환지수에 흡수되는 Ce(III), Tb(III) 및 $Cl^-$이온의 양을 흡광분광법으로 측정하였다. 염산-과염소산 혼합용액에서 Ce(III)와 Tb(III)의 %S(흡수백분율) 값은 염산용액의 값에 비하여 모든 산농도에서 증가하였으며, 혼산중 과염소산의 분율이 증가함에 따라 과염소산만의 %S에 접근하는 것을 관찰하였다. 또 $Cl^-$ 이온의 흡수를 염산의 농도에 따라 측정해 봄으로써 수지내에서는 희토류 양이온과 $Cl^-$이온간의 착물로 존재하는 양은 많아야 흡수된 전체 희토류 이온의 10%에 불과해서 수지내에서의 이러한 착물형성이 중요하지 않은 것을 알았다. 혼산중의 과염소산 분율이 증가함에 따라 수지내 착물형성의 경향이 더욱 감소하였으므로 %S의 증가현상은 Cl-이온보다는 $ClO_4^-$이온의 영향이 더 클 것으로 예상된다. To understand the abnormal absorption behavior of rare earths in cation exchange resins, the absorption for Ce(III), Tb(III) and $Cl^-$ ions in Dowex 50W-X2 have been investigated by spectrophotometry in the concentration range of $1{\sim}12$ M HCI and $HCl-HClO_4$ mixed solutions. The amount of $Cl^-$ ion absorbed shows that the ratio of amount of $Cl^-$ ions to that of rare earths does not exceed 10% in the concentration range of $6{\sim}8M$ HCl and decreased gradually to 3% at 2M HCl and 6% at 12M HCl. The ratio is further decreased with the fraction of $HClO_4$ in $HCl-HClO_4$ mixed solutions and the decrease is presumably due to the weak tendency to form a complex between rare earths and $Cl^-$ ions in a cation exchange resin. The effect of $ClO_4^-$ is expected to play a more important role than that of $Cl^-$ ions in the large absorption of rare earths.
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이범규 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.3
There is a continuous rise in the commer- cialization of living modified (LM) organisms worldwide. While LM plants have not yet been cultivated in South Korea, research, development, import of products, and registration of related research facilities are progressing. LM plants should be tested in greenhouses and fields during development. Furthermore, environmental risk assessment and safety management should be performed before their release into the environment. Research on LM plant develop- ment is conducted in laboratories as well as confined green- houses and fields. Safety management regulations are pro- vided as combination standards for the LMO Act in each research district. The accidental release of the LM petunia in Japan was a significant incident related to LM plant research. It implies that normal plants within the distance of crossing should be regarded as LM plants. In the United States, LM creeping bentgrass was released into the environment, thus necessitating the establishment of stringent measures to prevent the scattering of LM plant seeds by wind or other mediums. In South Korea, LM Zoysia and LM cotton were released through rainwater. Therefore, safety measures that prevent LM seed mixing and plant vegetative propagules escaping into the environment via rainwater must be established. Preventing the dispersal of unapproved LM plants requires significant time, expenditure, and effort. Researchers should first identify the impact of LM plants on the ecosystem, and steps to avert their environmental release must be implemented.
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