의약분업평가단의 역할과 기능

이규식,Lee, Gyu-Sik 대한병원협회 2001 대한병원협회지 Vol.30 No.3

출토 인골의 유전자분석-나주 복암리 3호분 옹관 인골을 중심으로

이규식,정용재,한성희,이명희,한면수,최동호,Lee, Kyu-Sik,Chung, Yong-Jae,Han, Sung-Hee,Lee, Myong-Hee,Han, Myun-Soo,Choi, Dong-Ho 국립문화재연구소 1999 保存科學硏究 Vol.20 No.-

We have analyzed the allele and genotype frequencies from 10 fractions of ancient human skeleton in 3 pieces of Jar coffin excavated from Naju Bokamni3rd tumulus by PCR amplification, high resolution polyacrylamide gelelectorphoresis and silver staining. We could isolate human genomic DNA from 3 bone fractions but the rest of them could not be used as materials due to being decayed. We could detect sex determination as male and 3 genotypes of STR system, HUMTHO1, HUMTPOX and HUMC5F1PO from the bone fraction of left side in Jar coffin 3 and see the slightly reaction suggesting the sex as male from the bone fraction of the left side in Jar coffin 2 and female from the right side in Jar coffin 3.We have also analyzed the genotype frequencies of mitochondria from the bone fractions of the left side and the right side in jar coffin 3, respectively. From the result of indetifiying at nucletide position between 16018 and 16378of the base of hyper variable region(HV1) in the control region, We can presume that the both bones have the same maternal inheritance.

원각사지 십층석탑 오염물의 유기산 분석

이규식,한성희,Lee, Kyu-Shik,Han, Sung-Hee 국립문화재연구소 1995 保存科學硏究 Vol.16 No.-

TEN-STORIED STONE PAGODA ON THE SITE OFWON-GAKSA(Temple) which is one of three marble pagodas in South Korea, were dated from the thirteenth year of the reign of King Sejo(1467). On the roof and surface of each the stories, there were large amount of the contaminants such as pigeon′s excretions, dust and environmental elements for a long time. The pH of contaminants is not acid, but is 7.2, neutral. To find the species of organic acidscontained in the contaminants and the degree of damaging for a marble pagoda, we analyzed the contaminants using GC-MSD method by the following procedures. Organic acids were extracted by saponifying whole contaminants. After Saponification, the organic acids were mathylated to increase their volatility upon subsequent GC-MSD analysis. The mathyl esters of the organic acids are extracted from the acidified aqueous solution. And the organic extracts were washed with adilute base solution. The washed extract were analyzed by GC(Hewlett Packard 5890)with a nonpolar capillary column(Crosslinked 5% Ph Me Silicone, $50×0.2㎜×0.33\mum$film thickness, USA) and Mass Spectrometric Detector(Hewlett Packard 5970B).As the result, it was found that 12 organic acids were the main compound in pagoda′contaminants, and the amount of organic acid were negligible.

목조건조물의 흰개미 모니터링 및 방제방법

이규식,정소영,정용재,Lee, Kyu-Shik,Jeong, So-Young,Chung, Yong-Jae 국립문화재연구소 2001 保存科學硏究 Vol.22 No.-

The wooden building is mainly damaged by the termite which have an effect of the structure by making emptied inner part of wood. One class, Japanese termite, inhabits throughout Korea and is often detected. So the deterioration by termite attacking the wooden building needs to be controlled. Termites are over 2,800 species in the world, usually they inhabit in the tropical or subtropical region and don’t over winter in dormant condition. So their activity and distribution are chiefly restricted by temperature and humidity. The termite inhabiting in Korea is Reticulitermes speratus kyushuensis Morimoto, which known to have an optimal temperature range at $12~30^{\circ}C$ and minimum temperature at $6^{\circ}C$ for activation. These temperatures correspond to the mean temperature($5.6^{\circ}C$~$25.8^{\circ}C$) from March to November and the activity time of termitein Seoul. In addition, as a result of environmental pollution by industrial development, the climate of Korea is getting warm. The increase of mean temperature in Korea has been $1.1^{\circ}C$ for the past seventy five years, so it is expected that the damage of wooden building by termite will increase gradually. Therefore, in order to protect wooden building from damages by termite, it needs not only development of new pest control methods, but also studies on the control of environmental factors having an effect on the activity and growth of termite. For the conservation of the large cultural properties such as the wooden building in the open air, it would be effective to use the methods of fumigation, insecticidal and antiseptic chemical treatment of wood materials, soil termiticideinjection treatment, and termite colony elimination system.

지류.섬유질 유물에 대한 대기유해가스($SO_2$, $NO_2$)의 영향

이규식,한성희,Lee, Kyu-Shik,Han, Sung-Hee 국립문화재연구소 1996 保存科學硏究 Vol.17 No.-

We exanmined the each effect of $NO_2$ and $SO_2$gas, the maincomponets of air pollutants, on the deterioration of 6 organic materials with Gasexposure cabinet. The organic materials were used 2 kind of papers(Korean paper, Oldbook paper) and 4 kind of textiles (Cotton, Silk, Hemp, ramie) in gas exposure experiments. In order to know how to change of physical conditions, the materials were exposed to 2000, 1000 ppm. h of $SO_2$dose, to 100 ppm. h of $NO_2$ dose at 65% RH. The color difference, tensile strength, elongation coeffient, mass reduction and fabric status of each materials were discussed the following below.1. The color difference of cotton and hemp was larger than that of silk and ramie in the presence of $SO_2$ gas. and the color difference of korean paper was less than that of the textile materials in the presence of $SO_2$ gas.2. The tensile strength of cotton fell suddenly at 100ppm.h of $SO_2$ dose and even became 56% of the unexposed cotton.3. The weight of 6 materials began to decrease in the presence of $SO_2$ gas.4. The tensile strength and elongation of the materials tended to decrease at 12.5ppm.h of $NO_2$ especially silk was the strongest tendency to decrease.5. Cotton, hemp and ramie were influenced by $SO_2$ gas more than by $NO_2$, but silk were influenced by $NO_2$ more thang by $SO_2$ at 10ppm.h of each noxious gas.

전력설비 예측정비기술

이규식,Lee, Gyu-Sik 대한전기협회 2009 전기저널 Vol.- No.385

한국의료 선진화를 위한 정책방안-2008년 새 패러다임 구축을 위한 보건의료개혁의 방향

이규식,Lee, Gyu-Sik 대한병원협회 2007 대한병원협회지 Vol.36 No.6

건강보험제도의 획기적인 개혁

이규식,Lee, Gyu-Sik 대한병원협회 2008 대한병원협회지 Vol.37 No.1

아데노바이러스 유전자치료벡터의 생식독성 연구

이규식,곽승준,김순선,이이다,석지현,채수영,정수연,김승희,이승훈,박귀례,Rhee, Gyu-Seek,Kwack, Seung-Jun,Kim, Soon-Sun,Lee, Rhee-Da,Seok, Ji-Hyun,Chae, Soo-Young,Chung, Soo-Youn,Kim, Seung-Hee,Lee, Seung-Hoon,Park, Kui-Lea 한국미생물학회 2007 미생물학회지 Vol.43 No.3

유전자치료W터의 주입시 생식세포를 통한 다음 세대로의 전달 가능성은 안전성 측면에서 관심을 중대시키고 있다. 특히 전립선암이나 난소암의 치료시 바이러스를 생식기관에 인접한 부위에 주입하여야 하므로 그 가능성이 높다. 따라서 본 연구에서는 유전자치료에 많이 이용되는 아데노바이러스를 매개로하여 tumor suppressor 유전자인 p53을 발현하는 아데노바이러스 벡터를 제조하여 이를 투여시 생식장기를 포함한 주요장기조직에의 분포와 germ cell을 통한 차세대로의 전달 가능성 등의 생식독성을 조사하였다. In vivo biodistribution study를 위하여 $Ad-CMV-{\beta}-gal$흑은 Ad-CMV-p53를 마우스 암 수의 복강에 주사한 후 생식장기를 포함한 주요 장기에서 아데노바이러스 유래 DNA검출 및 RNA발현 여부를PCR과 RT-PCR로 각각 확인하였다. 그 결과 간 및 비장과 같은 일반 장기에서도 주입한 외부유전자의 DNA가 검출되거나RNA가 발현되었을 뿐만 아니라, 정낭, 전립선, 부고환, 난소 및 자궁 등의 생식장기에서도 주입한 외부유전자가 검출되거나 발현되는 것으로 나타났다. Real-time PCR을 이용하여 각 장기에서의 투여된 아데노바이러스 벡터는 시간 의존적으로 감소되는 것을 정량하였다. Ad-CMV-p53를 암 수 마우스의 난소와 고환에 각각 직접 주사하여 교배시킨 후 그 후세대의 DNA를 분리하여 주입한 아데노바이러스 유래의 DNA를 검색한 결과, 어떠한 차세대에서도 주입한 아데노바이러스 유래의 DNA가 검출되지 않았다. 한편 생식장기에서의 PCR및 RT-PCR signal유래 vector의 위치를 확인하기 위해 매우 감도가 높은 in-situ PCR로 조사한 결과 고환의 경우 간질조직으로의 전달은 일어나나 정세관 내에는 아데노바이러스 벡터가 전달되지 않으며, 난소에서도 아데노바이러스벡터는 난포내의 난자에 전달되지 않고 기질조직에 존재하는 것으로 확인되었다. 결론적으로 복제 능력 이 결여된 아데노바이러스를 매개로 한 유전자치료제는 생식 장기에서 검출되더라도 다음 세대로 전달될 가능성은 대단히 낮음을 제시한다. The possibility of inadvertent introduction of therapeutic gene expressing viral vectors has raised safety concerns about germ-line infection. Particularly, for indications such as prostate cancer and ovarian cancer, the proximity of the point of viral administration to organs of the reproductive system raises concerns regarding inadvertent germ-line transmission of genes carried by the virus vector. To evaluate the safety of in vivo adenovirus mediated gene transfer, we explored the biodistribution, persistance and potential germ-line transmission of p53-expressing adenovirus (Ad-CMV-p53). Both male and female Balb/c mice were injected with $1{\times}10^9$ PFU of Ad-CMV-p53. The PCR analysis showed that there were detectable vector sequences in liver, kidney, spleen, seminal vesicle, epididymis, prostate, ovary, and uterus. The RT-PCR analysis for detecting inserted gene, p53 showed that Ad-CMV-p53 viral RNA were present in spleen, prostate and ovary. Direct injected male and female mice of adenovirus vector into testis and ovary were mated and their of offspring were evaluated for germ-line transmission of the adenoviral vector. The PCR and RT-PCR analysis showed no evidence of germline transmission, although vector sequences were detected in DNA extracted from gonadal tissues. Real-time PCR result confirmed a significant decrease of adenovirus in gonad tissues 1 week after injection. We have also analysed the cell specific localization of viral DNA in gonad tissues by using in-situ PCR. Positive signals were detected in interstitial tissue but not in seminiferous tubule in sperm. In the case of ovary, adenovirus signal were localized to the stromal tissue, but no follicular signals were observed. Together, these data provide strong evidence that the risk of the Inadvertent germ-line transmission of vector sequences following intraperitoneal or direct injection into genito-urinary system of adenovirus is extremely low.

목조문화재의 원형보존을 위한 충해 방제방안

이규식,정소영,정용재,Lee, Kyu-Sik,Jeong, So-Young,Chung, Yong-Jae 국립문화재연구소 2000 保存科學硏究 Vol.21 No.-

The cultural properties are damaged by various causes according to the characteristics of material, the condition of preservation, and the period of time. Especially, biodeterioration makes lots of damages in organic properties than inorganic ones. The damages of wooden cultural properties by insects usually are caused by the three orders; Isoptera, Coleoptera, and Hymenoptera. As the result of investigation on the state of 141 buildings of wooden cultural properties in 1999, some of them were damaged by many kinds off actors; wasp, powder post beetle, cigarette beetle, termite, decay, and physical cracking. And it was found that the patterns of damages were related to species-specific habits of insects. There are several methods of pest control for the prevention of wooden cultural properties from damages caused by insects. Those are as follows; physical control, chemical control, biological control, and integrated pest management. When insects and fungi were detected at the wooden buildings, the fumigation is best treatment to stop biodeterioration. And then, wood materials also need to be treated with insecticidal and antiseptic chemicals to avoid a reinfestation, because the fumigant is volatile. The six commercial chemicals which are applied to the insecticidal and antiseptic treatment of wooden cultural properties were purchased to test their abilities. According to the comparative results of efficacy of them in laboratory, chemical D showed excellent efficacy in all items, including antiseptic and termiticidal items. The goal of these pest controls is to protect wooden buildings from insects and microorganisms. The most effective method used currently is chemical control(fumigation, insecticidal and anticeptic chemical treatment), but it has to be treated periodically to control pest effectively. Recently environmentally-friendly control methods such as bait system or biological treatments are replacing traditional barrier treatments using large amounts of chemicals. Especially, termite is a social insect which makes a colony. Although a building with fumigation treatment is safe for a while, once attacked building has a risk of damage by reinfestation of termite. Therefore, to control termites from damaged building, the entire colony including reproductives(queen and king) and larvae around buildings must beeliminated. Bait system can be used as a preventive measure in early detection of them through termites colony monitoring and baiting. It would be the most effective for termite control if bait system would be used together with the chemical controls.