콜라겐 젤과 성장인자 전처치 및 피복재료가 다공성안와삽입물의 섬유혈관증식에 미치는 효과

이준영,김석영,유창국,이무석,이상렬,오중협,김성주.Joon-Young Lee. M.D.. Suk-Young Kim. Ph.D.. Chang-Kook You. Moo-Seok Lee. Sang-Yeul Lee. M.D.. Ph.D.. Jung-Hyub Oh M.D.. Ph.D.. Sung-Joo Kim M.D.. Ph.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.6

Purpose: To determine the effect of bFGF complexed collagen gel, which allows constant release of bFGF along with biodegradation of the collagen gel. The specific study purpose was to determine whether it can accelerate the fibrovascular ingrowth into wrapped HA-coated porous alumina and to verify the safety of new wrapping materials. Synthetic polyester-urethane (Neuropatch?) and lyophilized bovine pericardium (Lyoplant?) were compared to donor sclera for the fibrovascular ingrowth into HA-coated porous alumina. Methods: The experimental and control groups, each consisting of 9 rabbits were wrapped with each wrapping materials (3 rabbits per wrapping material). The experimental group underwent pretreatment of bFGF-collagen gel while the control group did not. The fibrovascular ingrowth was compared at 2 and 4 weeks after implantation. Western blot analysis was conducted at 4 weeks using antibodies against CD141 and laminin. The rate of fibrovascular ingrowth was fastest in orbital implant wrapped with Lyoplant?. Results: Histopathologic examinations at 2 weeks showed no differences in distance and percentage area of fibrovascular ingrowth. Histopathologic examinations at 4 weeks showed that pretreatment of bFGF-collagen gel increased the fibrovascular ingrowth in the experimental group. Western blot analysis on experimental group also showed that the expressions of CD141 and laminin were increased by bFGF-collagen gel, thereby indicating that the fibrovascular proliferations were accelerated by bFGF released from the complex. Conclusions: bFGF-collagen gel increased the rate and degree of fibrovascular growth into hydroxyapatite- coated porous alumina by releasing bFGF as the collagen gel biodegraded. Both Lyoplant? and Neuropatch? were evaluated as safe for substitution of the donor sclera.

자가혈청하에서 FGF-2와 덱사메타손에 의한 골수중간엽줄기세포의 증식과 분화에 대한 효과

손민정 ( Min Jung Shon ),이선영 ( Sun Young Lee ),김태호 ( Tae Ho Kim ),유창국 ( Chang Kook You ),김석영 ( Suk Young Kim ),정필훈 ( Phil Hoon Choung ),손영숙 ( Young Sook Son ),박의균 ( Eui Kyun Park ),김신윤 ( Shin Yoon Kim ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.4

Previously we have shown that heat-inactivated autologous serum (HAS) has a potential to stimulate proliferation and ostoblastic differentiation of bone marrow stromal cells (BMSCs). In the present study we investigated whether stimulatory effects of HAS on proliferation and osteoblastic differentiation of BMSCs are further potentiated by fibroblast growth factor-2 (FGF-2) and dexamethasone (Dex). As expected, FGF-2 and Dex stimulated proliferation of BMSCs up to 17% at 5 days and 26% at 7 days of culture compared to HAS control. These results suggest that FGF-2 and Dex in the presence of HAS further stimulate proliferation of BMSCs. In order to examine whether BMSCs expanded with FGF-2, Dex and HAS harbor multipotency, the expanded cells were stimulated with either osteogenic or adipogenic cocktails. BMSCs expanded with FGF-2, Dex and HAS for 7 days were able to be differentiated into either osteoblasts or adipocytes. Taken together, these results demonstrate that FGF-2 and Dex in combination with HAS further stimulates proliferation of BMSCs and these expanded cells maintain potentials to be differentiated into either osteoblasts or adipocytes.

혈관내피성장인자가 사람 지방간엽줄기세포와 골수간엽줄기세포의 골재생 유도에 미치는 영향

임지원 ( Ji Won Lim ),노혜정 ( Hey Jeong Noh ),양정호 ( Jung Ho Yang ),유창국 ( Chang Kook You ),윤희숙 ( Hui Suk Yun ),신홍인 ( Hong In Shin ),김신윤 ( Shin Yoon Kim ),박의균 ( Eui Kyun Park ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s

Human adipose tissue-derived stromal cells (ATSCs) and human bone marrow stromal cells (BMSCs) are promising stem cell sources for bone regeneration. However, implantation of ATSCs or BMSCs alone induces partial effect on bone formation. Angiogenesis and blood supply are essential in tissue survival after implantation. We investigated effects of vascular endothelial growth factor (VEGF), a well known angiogenic factor, on ATSCand BMSC-induced bone regeneration. In vitro effects of VEGF on the proliferation (MTS assay) and osteogenic differentiation (ALP and Alizarin Red staining) of ATSCs and BMSCs were examined. ATSCs and BMSCs were seeded on biphasic calcium phosphate (BCP), treated with VEGF, and implanted on calvarial defects of nude mice. Bone regeneration was assessed by Micro-CT and histology at 10 weeks after implantation. Proliferation and differentiation of ATSCs and BMSCs was not increased by VEGF in vitro. However, BMSCs and ATSCs treated with VEGF showed increased bone mineral density (BMD) and bone mineral content (BMC) as assessed by micro-CT. In addition, histomorphometric analysis showed that new bone formation was significantly increased in VEGFtreated ATSCs (30%) and BMSCs (21%) groups compared to untreated group. These results demonstrated that adult MSCs (ATSCs and BMSCs) treated with VEGF can promote bone regeneration in calvarial critical-sized defect model. Taken together, VEGF can be a useful factor for bone repair and regeneration.

Biodegradable Calcium Phosphate Bone Cement Incorporated with Antibiotics

Min-Ho Hong(홍민호),Kwang-Mahn Kim(김광만),Kang-Sik Lee(이강식),Chang-Kook You(유창국),Yong-Keun Lee(이용근) 대한치과재료학회 2011 대한치과재료학회지 Vol.38 No.4

본 논문에서는 신생골을 재생할 수 있고, 항균력을 갖는 골 시멘트에 관하여 연구하였다. 시멘트는 비정질 칼슘 포스 페이트 유리를 분말로 사용하고, 염기성 용액과 산-염기 반응하여 경화하였다. 항생제는 아미카신, 테트라사이클린 및 반코마이신을 사용하였으며, 경화 중에 열이 발생되지 않기 때문에 항생제를 변성없이 시멘트에 혼합할 수 있었다. 경화 후 시멘트의 물리화학적 특성과 칼슘 이온 및 항생제의 방출 속도를 측정하였으며, 항생제의 변성 여부를 확인하기 위하여 항균시험을 진행하였다. 항생제를 변성없이 최적량을 담지할 수 있음을 확인하였으며, 항생제의 종류와 첨가량에 따라 기계적 성질은 달라짐을 알 수 있었다. 항생제 방출 속도 시험결과 모든 군에서 초기 과다 방출현상을 보였는데, 항생제 담지법이 다르지 않는 이상 형태학적인 측면에서 보았을 때, 그 현상은 피할 수 없는 것으로 판단되었다.

조직공학용 스캐폴드의 강도 향상

김성민(Sung-Min Kim),김광만(Kwang-Mahn Kim),이강식(Kang-Sik Lee),유창국(Chang-Kook You),이용근(Yong-Keun Lee) 대한치과재료학회 2011 대한치과재료학회지 Vol.38 No.4

Calcium phosphate such as Hydroxyapatite (HA) and beta tri-calcium phosphate (β-TCP), due to its excellent biocompatibility and bioactivity, is widely used in the clinic of orthopedic as well as dental applic ations. The HA h as g ood biologic al p roperties like n on-immunogenicity, n on-inflammatory behavior, high osteoconductivity and osteoinductivity. The β-TCP shows the good biocompatibility and biodegradability. Especially, calcium phosphate scaffolds have interconnected pores that provide a favorable environment for bone i ngrowth, o sseointegration a nd b lood s upply. T he c alc ium phosphate sc affold, however, h as brittle property and low strength. The purpose of this study was to investigate the effects of a kind and each amount of binder and fabrication methods for high compressive strength of calcium phosphate scaffold. At HA scaffold, the maximum compressive strength was performed when combined binder of 3 wt% of polyvinyl alcohol and 1 wt% of methyl cellulose was selected. The β-TCP scaffold fabricated by novel template- casting has high compressive strength than sponge method.

XPS and FT-IR Study on BMP Coated Scaffolds for Bone Tissue Engineering

김성민(Sung-Min Kim),김광만(Kwang-Mahn Kim),이강식(Kang-Sik Lee),유창국(Chang-Kook You),이용근(Yong-Keun Lee) 대한치과재료학회 2012 대한치과재료학회지 Vol.39 No.1

수산화 아파타이트 스캐폴드를 스펀지 방법을 이용하여 제조 하였다. 제조한 수산화 아파타이트 스캐폴드를 침적코팅을 이용하여 표면에 젤라틴으로 코팅 하였다. 젤라틴을 코팅한 수산화 아파타이트 스캐폴드에 두 가지 다른 코팅 방법인 침적코팅과 항원-항체반응를 이용하여 골형성단백질을 코팅하였다. 제조한 스캐폴드의 상분석과 생물학적 특성을 코팅하지 않은 순수 수산화 아파타이트 스캐폴드와 비교, 분석 하였다. Fourier transform infrared spectroscopy와 X-ray photoelectron spectroscopy를 이용하여 상분석을 하였고, 세포의 증식 및 분화를 측정 하기 위해 cell counting kit와 alkaline phosphatase시험을 진행 하였다. 상분석 결과 수산화 아파타이트 표면에 젤라틴 및 골형성 단백질이 코팅이 되었다는 것을 알 수 있었으며, X-ray photoelectron spectroscopy결과에서 탄소와 칼슘의 양을 확인하여 침적코팅에 비해 항원-항체반응을 이용한 코팅 방법에서 골형성 단백질이 더 잘 코팅 되었다는 것을 확인 할 수 있었다. 또한, cell counting kit와 alkaline phosphatase시험에서 골형성 단백질을 코팅한 수산화 아파타이트 스캐폴드가 세포의 증식 및 분화에서 더 좋은 결과를 나타내는 것을 알 수 있었으며, 특히 3일 이후부터, 세포 분화에서는 항원-항체 반응을 이용한 코팅 방법에서의 스캐폴드에서 더 높은 세포 분화를 확인 할 수 있었다. 이 연구를 통해 수산화 아파타이트 스캐폴드에 골형성 단백질을 코팅 할 경우, 침적코팅 방법 보다 항원-항체 반응을 이용하여 코팅 했을 때, 세포의 분화가 더 향상 된다는 것을 알 수 있었다.