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- 저자 : 안응남 ( An Eung Nam ) (검색결과 28 건)
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운동과 리포산 투여가 노화 유도 쥐의 해마에서 Caspase-3 및 Mn-SOD, Cu, Zn-SOD 발현에 미치는 영향
강성환(Kang, Sung-Hwan),안응남(An, Eung-Nam) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.2
In this study, we investigated the effects of exercise and DL-α-lipoic acid on caspase-3, Mn-superoxide dismutase(SOD), and CU,Zn-SOD levels in hippocampus of aged rats induced by D-galactose. Forty male Sprague-Dawley(SD) rats were divided into four goups: aging/control group(ACG, n=10), aging/exercise group(AEG, n=10), aging/lipoic acid group(ALG, n=10), aging/exercise/lipoic acid group(AELG, n=10). After dividing groups, all groups were injected D-galactose(50㎎/㎏/day) during 8weeks. ALG and AELG were injected DL-α-lipoic acid(100㎎/㎏/day) and ACG was injected vehicle. Rats were subjected to treadmill exercise for 5days a week over 8 weeks, and the speed of the treadmill was gradually increased. For results, significant increases in Mn-SOD protein levels were observed in the hippocampus of AEG, ALG and AELG compared to ACG(p<.05). CU,Zn-SOD protein levels significantly increased in the AEG and AELG compared to ACG(p<.05). Caspase-3 protein levels significantly decreased in the AEG, ALG and AELG compared to ACG(p<.05). Especially, AELG was significantly increased better than others in all protein levels(p<.05). These results show that exercise combined with DL-α-lipoic acid is more effective to improve Mn-SOD, Cu,Zn-SOD and caspase-3 levels in hippocampus of aged rats induced D-galactose.
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트레드밀 운동과 L-arginine 투여가 고지방식이 흰쥐의 성장호르몬, 체중 및 복부지방에 미치는 영향
우도영(Do Young Woo),안응남(Eung Nam An) 한국체육측정평가학회 2010 한국체육측정평가학회지 Vol.12 No.2
본 연구에서는 성장호르몬 대사에 관여하는 유산소 운동과 L-arginine에 대한 효과를 알아보기 위하여 고지방 식이 섭취로 유도된 비만 흰쥐에서 트레드밀 운동과 L-arginine (150 mg/kg) 투여가 체중, 식이섭취량, 성장호르몬, 그리고 복부비만에 미치는 영향을 관찰함으로써 비만에 대한 효과를 알아보고자 하였다. 본 연구의 대상은 6주령 된 Sprague-Dawley계 수컷 흰쥐 50마리를 구입하여 32마리를 무작위 추출하여, 지방이 전체 칼로리의 60%를 차지하는 고지방식이사료(high fat diet # D12492, Reserh Diets Inc., USA)를 섭취한 고지방식이군(high fat diet; HF)을 대조집단으로 하여 트레드밀 운동(EX)과 L-arginine 투여(LA)가 체중 및 지방대사에 미치는 영향을 알아보기 위하여 HF군(n=8), HF+EX군(n=8), HF+LA 섭취군(n=8) 및 HF+EX+LA 섭취군(n=8)의 각 4개 군으로 분류하여 12주간의 실험을 실시하였다. 체중의 변화와 식이섭취량은 사전, 4주, 8주, 12주를 측정하여, 집단 및 시기 간의 이원반복변량분석을 적용하였으며, 그룹별 성장호르몬 농도와 복부지방량 측정은 일원변량분석을 적용하였고, 사후검증으로 Tukey`s 테스트를 적용하였다. 통계적 유의수준은 5%를 기준으로 하였다. 식이섭취량에서는 각 실험집단의 차이가 없었으나, 체중은 트레드밀 운동집단과 L-arginine 투여집단 그리고 L-arginine 투여와 트레드밀 운동을 실시한 복합처치집단 모두 유의하게 감소하였다. 또한 복부지방 조직 무게에서도 트레드밀 운동집단과 L-arginine 투여와 트레드밀 운동을 병행한 복합처치집단에서 유의하게 감소하였다. 혈청 성장호르몬 농도는 대조집단에 비하여 트레드밀 운동집단과 L-arginine 투여집단 그리고 L-arginine 투여와 트레드밀 운동을 실시한 복합처치집단 모두에서 유의하게 증가하였다. 결론적으로 트레드밀 운동과 L-arginine 투여가 성장호르몬의 농도를 증가시키는 방법으로 체중 및 지질 대사에 영향을 미쳐 체지방 형성을 억제함으로써 항비만 효과를 나타낼 수 있을 것으로 사료된다. 또한 트레드밀 운동과 L-arginine 투여를 병행할 경우에 복부지방량에서 단독 투여군에 비하여 더 많은 감소를 보여 운동과 L-arginine 투여의 병행이 체지방 감소에 보다 긍정적으로 작용할 수 있을 것으로 사료된다. The aims of this investigation was to examine the anti-obesity effects of treadmill exercise and oral administration with L-arginine in the high-fat diet induced obestiy rats. It is to analysis changes in body weight, food intake, abdomianl fat and growth hormone. Experimental rats were divided into 4 different experimental groups incluing an HF (high fat control; n=8), HF+LA (high fat+L-arginine oral administration; n=8), HF+EX (high fat+treadmill exercise), and HF+LA+EX (high fat+L-arginine oral administration+tredmill exercise; n=8). Rats were fed experimental diets supplemented with L-arginine at the level of 150 mg/kg/day for 4 weeks. Rats of HF+EX group and HF+LA+EX group performed tredmill exercise training at 60 min/day for 12 weeks. The body weight and abdominal fat mass were significantly decreased in HF+LA, HF+EX, and HF+LA+EX compared with HF group despite that food intake was not differed amone the experimental groups. However, The plasma growth hormone was significantly increased in HF+LA, HF+EX, and HF+LA+EX compared with HF group. These results suggested that the L-arginine oral administration and the regular aerobic exercise made the increase of anti-obesity effect in high-fat diet induced obesity rats.
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제지방체중에 의한 최대산소섭취량 판정의 타당성에 관한 연구
최성근(Choi Sung Keun),안응남(An Eung Nam) 경희대학교 스포츠과학연구원 1994 체육학논문집 Vol.22 No.-
The purpose of this study is to examine the validity in evaluation of maximal oxygen uptake by the lean body mass. Through tests and analyses of ten woman object students attending S college, we can draw following conclusions:<BR> 1) In evaluation of maximal oxygen uptake by the lean boby mass, the analised amount of maximal oxygen uptake is overestimated in case the rate of body fat is high.<BR> 2) Even in case of low scored physical fitness, the overestimated amount of maximal oxygen uptake per lean boby mass leads to misinterpretation of physical fitness.<BR> Consequently it follows that the evaluation of maximal oxygen uptake by the lean body mass dose not provide any useful variable in estimation of physical fitness, great care should be taken in applying to the analysis of exercise performance.
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지구성 운동시 당뇨병 흰쥐의 SOD, GSH-Px 활성도 및 산화 LDL의 변화
김현태 ( Kim Hyeon Tae ),안응남 ( An Eung Nam ) 한국운동영양학회 2003 Physical Activity and Nutrition (Phys Act Nutr) Vol.7 No.1
The purpose of this study was investigated the effect of 30minutes endurance exercise (16-17m/min) on vitamin E, selenium concentration. SOD, GSH-Px activity and oxidized LDL in streptozotocin induced diabetic rats. It were divided diabetes (n=12) and control group (n=12) for antioxidant capacity. The results were obtained which were analyzed vitamin E, selenium in liver, SOD, GSH-Px activity in mitochondria of liver and oxidized LDL in rest and post-exercise of each group, exercise time. The results of this study was summarized as the follows. Blood glucose and vitamin E, selenium concentration in liver showed significant differences. SOD, GSH-Px activity in mitochondria of liver showed any significant differences between two groups. Each exercise time, diabetes group in SOD decreased in post-exercise but control group increased GSH-Px of all group decreased any significant in post-exercise. When LDL was oxidized by each times showed any significant differences between two groups except 60minutes. Also, it was showed that diabetes group is decreased in post-exercise test. These results indicate that diabetes group was showed specific decrease antioxidant defense system by free radical of hyperglycemia. Especially 30minutes endurance exercise was decreased blood glucose concentration, Furthermore it was examined the effect of oxidized LDL which arteriosclerosis index decreased any significant. Future study is required to multiples research the effect of antioxidant and exercise teratment in diabetic rats.
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채창훈 ( Chang Hun Chae ),안응남 ( Eung Nam An ) 한국운동영양학회 2005 Physical Activity and Nutrition (Phys Act Nutr) Vol.9 No.1
The purpose of this study was to investigate the effect of long-term L-Carnosine Intake and regular aerobic exercise on blood MDA concentration and SOD, GPx, CK, LDH activity, regarding oxidative stress during exercise test for 30min (running at 16-17min). The rats were assigned randomly to 4 groups (control, n = 7; camosine intake, n = 7; exercise, n = 7; exercise + camosine intake, n = 7). Each regimen was 8 weeks long. MDA concentrations in exercise + camosine intake group was significantly lower compared to control group. SOD activity in exercise + carnosine intake group was significantly higher compared to the groups. GPx activity in exercise + camosine intake group was significantly higher compared to control group. CK activity in exercise and camosine intake, exercise + carnosine intake groups was lower compared to control group. LDH activity in exercise + carnosine intake group was significantly lower compared to the groups. These results indicated that long-time regular aerobic exercise and camosine intake may inhibit lipid peroxidation to oxidative stress and increase activity of SOD and GPx, but not CK and LDH
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