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기능성 식품 자원의 지질, 아미노산 및 식이 섬유의 조성 - 길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 -
황성원(Sung-Won Hwang),박무희(Moo-Hee Park),심호기(Ho-Ki Shim),배만종(Man-Jong Bae) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4
길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 등의 식이 섬유소, 지방산 조성 및 염용성 단백질의 아미노산을 분석 비교한 결과는 다음과 같다. 식이 섬유로서 neutral detergent fiber(N.D.F), acid detergent fiber(A.D.F), lignin, hemicellulose, cellulose 등의 함량은 달맞이꽃 종자가 cellulose를 제외하고는 많은 함량이었다. 총지질의 지방산조성에서 P/S 비율은 들깨 종자가 6.31로 높았으며, linolenic acid (n-3)는 들깨 종자에 있어 55.47%, linoleic acid (n-6)는 달맞이꽃 종자에 71.88%로 그 함유비가 높았다. 중성지질의 지방산 조성은 총지질의 지방산 조성과 비슷하였다. 당지질의 지방산 조성에서 PUFA의 함유비는 들깨 종자 61.76%, n-6/n-3 비율은 달맞이꽃 종자가 15.19이었다. 인지질의 지방산 조성은 당지질의 지방산과 조성과 비슷하며, 길경의 PUFA의 함량은 62.96%로 약간 증가되었다. 염용성 단백질의 아미노산 조성에서 필수아미노산은 길경이 47 ㏖e%로 높은 함유비를 나타내었으며, E/N 비율은 길경이 0.9, 알로에 베라가 0.66이었다. This study was conducted to investigate the contents of dietary fiber(DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food sources such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F), acid detergent fiber (A.D.F), lignin, hemicellulose and cellulose in evening primrose seed were higher than those of other samples, except the content of cellulose. The ratio of polyunsaturated/saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88%, which was higher than those of other samples. The fatty acid compositions in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the same as those of glycolipids. The contents of PUFA in Platycodi radix were 62.96%. The essential amino acid contents of salt-soluble protein were 47mole% in Platycodi radix, which was slightly higher than those other samples. The ratio of essential amino acid / nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.
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레지오넬라 폐렴의 진단용 바이오마커의 발굴 : A/J 마우스 감염 모델에서 Legionella pneumophila의 독력 유전자들의 발현양상 분석
김승민,심희선,김희남,심호기,윤영경,김정연,박윤선,박대원,손장욱,김민자 대한감염학회 2010 감염과 화학요법 Vol.42 No.1
Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www.tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified.
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