一部再修生들의 行態와 心身健康狀態와의 關係

申重圭,李誠 國 대한보건협회 1984 대한보건연구 Vol.10 No.2

A survey was carried out on physical and mental unidentified complaints of 544 male repeaters who regularly attend a lecture in a preparatory school using a questionaire from March 10 to May 10, 1984. The unidentified complaints were evaluated by means of their behavior patterns such as sleeping condition, smoking, drinking, use of drugs and meal patterns. The purpose of this investigation is to determine the relationship between their behavior patterns and level of unidentified complaints. The results obtaind are summarized as follows: The level of unidentified complaints was classified into 3 groups: A group(under 5 items), B group (6~11 items), C group(12 items and over). Average number of unidentified complaints accounted for 3.7±1.3 items for A, 8.6±1.6 items for B, 12.6±0.9 items for C, and 8.1±3.1 items for the total. The higher frequency rate of complaints revealed 84.0% of "fatigue", 79.0% of "eye-strain", 64.3% of "headache", in physical complaints, and 73.7% of "depression", 67.5% of "anxiety", 55.7% of "inability to concentration", 51.1% of "irritability", in mental complaints. There was statistically significant between unidentified complaints and behavior patterns such as sleeping condition, sleeping hours, and use of drugs (p<0.01). Those who slept uncomfortable(27.8%), 8 hours and over(18.9%), and using a sedative(27.6%) had relatively more complaints in C group. The relationship between unidentified complaints and meal patterns also was statistically significant (p<0.01). Those who had frequent lack of breakfast and lunch, irregural dinner(18.7%) had relatively more complaints in C group. The better physical-mental health status appears to be affected by the better eating behavior. In summarizing, the result of the survey clearly indicates that there would be significant relationship between behavior patterns and physical-mental health status on repeaters.

染色공장의 염료의 비산 관리

신중규 한국염색가공학회 1995 韓國染色加工學會誌 Vol.7 No.2

흰쥐에 있어서 Bromobenzene전처치가 간조직 중 Glutathione 및 Glutathione S-transferase활성에 미치는 영향

신중규 한국환경보건학회 1997 한국환경보건학회지 Vol.23 No.2

To evaluate the effect of bromobenzene pretreatment on the bromobenzene metabolism, the animal group was induced the stage of slight liver damage with 7 times bromobenzene injection every two days (400 mg/kg body wt. i.p.). In the present experimental animal model, the single dose of bromobenzene(400 mg/kg body wt. i.p.) was injected to the bromobenzene-pretreated rats and the hepatic aniline hydroxylase(AH) activity, glutathione(GSH) content and glutathione S-transferase (GST) activity were determined at the intervals of 2, 4, 8, 24 hours throughout 24 hr. The activities of hepatic AH and GST were generally higher in bromobenzene-pretreated rats than those in normal group throughout the whole course of experiment. Furthermore, the decreasing rate of hepatic GSH content was also higher in bromobenzene pretreated rats than in normal rats. Moreover, the value of V$_{max}$ in hepatic GST was higher in bromobenzene pretreated rats than that in the normal rats. In conclusion, these results indicate that the pretreatment of bromobenzene may rather enhance the bromobenzene metabolism.

공무원 및 교직원의 HBsAg 및 Anti-HBs 양성율과 전이 효소 활성치와의 관련성

신중규 대한보건협회 1988 대한보건연구 Vol.14 No.1

This study was carried out in Taegu city for the some public officials and school personnel population to find out the prevalence of HBsAg and Anti-HBs among population, and particulary the relationship between the presence of HBsAg and liver function tests (AST, ALT) from April to July 1986. The HBsAg was detected in 91 among 878 with positive rate of 10.4%. The positive rate of HBsAg was higher in male (11.9%) than in female (7.3%). The positive rate of Anti-HBs was 33.5% and it was higher in male (34.3%) than in female (32.0%). Elevated AST (>40IU/l) and ALT (>35IU/1) were higher in HBsAg positives (respectively 11.0%, 12.1%) than that of Anti-HBs positives (respectively 6.7%, 7.1%) and both negatives (respectively 4.6%, 5.2%). The mean values of AST and ALT in HBsAg and Anti-HBs positives showed higher than that of both negatives.

Effect of Cyclohexane and Xylene Mixture Treatment on the Liver Damage in Rats

신중규 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.2

To investigate the cyclohexane and xylene mixture treatment on the liver damage, the rats were treated by the mixture of cyclohexane and xylene (CH+X) and then, liver damage was demonstrated by liver function findings based on liver weight/body weight, serum level of alanine aminotransferase (ALT), xanthine oxidase (XO) and then compared with cyclohexane treated group (CH group) and xylene-treated group (X). The CH+X group showed merely severer liver damge than CH or X group. On the other hand, CH+X group showed lower activity of hepatic cytochrome P-450 dependent aniline hydroxylase (CYPdAH) than CH or X group, but no statical differences were demonstrated among three experimental groups. Especially the hepatic GSH content was merely declined than CH or X group and the activity of hepatic GST was higher in CH+X group than CH or X group. In conclusion, cyclohexane and xylene mixture treated animals showed merely severer liver damage than cyclohexane or xylene treated group and such a fact may be caused by inhibition of cyclohexane or xylene metabolism and oxygen free radical.

흰쥐에 있어서 간손상 정도에 따른 Bromobenzene 대사

신중규 한국독성학회 1997 Toxicological Research Vol.13 No.4

To compare the severe liver damage with the slight one on the bromobeazene metabolism in rats, the animal group described as B7 group was induced the stage of slight liver damage with 7 times bromobenzene injection every other day (400 mg/Kg body wt. i.p.), whereas B40 group was induced that of more severe liver damage with bromobeazene 40 times injection as identified with determination of serum levels of alanine aminotransferase(ALT) activity and the histopathological findings. In the present experimental animal model, the decreasing rate of glutathione(GSH) and the increasing rate of glutathione S-transferase activity to the control group were higher in B7 group than B40 group. Furthermore the single dose of bromobenzene was injected to the two groups and sacrificed at 8hr and the hepatic aniline hydroxylase(AH) activity, GSH content and GST activity were determined. The increasing rate of AH activity to the control was lower in B40 group than B7 group and the decreasing rate of GSH to the control was also lower in B40 than B7 group. Moreover, B7 group showed the increased activity of hepatic GST to the control whereas B40 group showed the decrease activity of the enzyme. And Vmax value in GST was more decreased in B40 group than B7 group.

흰쥐에 있어서 Toluene대사에 미치는 주.야 시차의 영향

류종일,윤종국,신중규 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1999 Journal of biomedical laboratory sciences Vol.5 No.1

주·야 (day, night phase) 시차에 따른 toluene 대사를 검토할 목적으로 흰쥐에 50% toluene (olive oil과 toluene의 동량혼합액)을 체중 100 g 당 0.2 ml씩 밤 12시, 낮 12시에 각각 투여한 다음 각각 8시간 후에 처치하여, 다음과 같은 결과를 얻었다. 간 microsomal aniline hydroxylase 활성은 night phase군이 day phase군 보다 대조군 및 toluene투여군 모두 높게 나타났으며, cytosol의 benzylalcohol dehydrogenase (BADH) 활성은 night phase군과 day phase군 간에 별다른 차이를 볼 수 없었으나, toluene 투여시에는 night phase군이 day phase군 보다 효소의 활성도가 오히려 억제되었다. 또한 간 조직의 benzaldehyde dehydrogenase (BALDH) 활성은 night phase군이 day phase군 보다 다소 높게 나타나는 경향을 보였으며, toluene 투여시에는 night phase군에서는 대조군 보다 오히려 감소되었으나 day phase군에서는 높게 나타나는 경향을 보였다. 그리고 in vitro 시험에서 benzylalcohol 및 benzaldehyde가 BADH 및 BALDH 활성을 억제시킴이 관찰되었다. 요 중 hippuric acid 함량은 대조군 및 toluene 투여군 모두 night phase군과 day phase군 간에는 별다른 차이를 볼 수가 없었다. 한편 toluene투여로 인한 체중 당간 무게 및 혈청 xanthine oxidase 활성 증가율은 night phase군이 day phase군 보다 높게 나타났다. 이상 실험결과를 종합해 볼 때 요 중 hippuric acid 함량이 주·야 시차에 따라 별다른 차이가 없는데도 불구하고 toluene으로부터 benzylalcohol 및 benzaldehyde 생성률이 day phase군 보다 night phase군이 높게 나타남으로서 toluene 대사산물에 의한 간 손상 정도가 day phase군 보다 night phase군이 높게 나타난 것으로 사료되어진다. To investigate the effect of the circadian variations on the toluene metabolism, 50% toluene in olive oil (0.2ml/100 g body weight) was intraperitoneally administered to the rats every other day for 6 days both in the night; 24:00 and the day; 12:00. Each group of animals was sacrificed at 8 hr after last injection of toluene. Hepatic microsomal aniline hydroxylase activity was more increased in control rats of night phase than those of day phase. On the other hand, the activities of hepatic benzylalcohol dehydrogenase in control rats of night phase showed the similiar value with that in those of day phase and in case of toluene treatment, these enzyme activities in rats of night phase were rather more decreased than those of day phase. Furthermore, hepatic benzaldehyde dehydrogenase activities were more or less higher in the control rats of night phase than those of day phase and by toluene treatment, enzyme activities of rats of night phase were somewhat decreased than those of day phase. In vitro, benzylalcohol or benzaldehyde inhibited the activities of benzylalcohol or aldehyde dehydrogenase prepared from the rats liver supernatant. There were no differences in urinary hippuric acid contents between the night phase and day phase both in the control and toluene treated group. The increasing rate of liver weight per body weight (%), serum xanthine oxidase activities were higher in rats of night phase than in those of day phase by toluene treatment. In conclusion, these results indicate that the producing rate of benzylalcohol and benzaldehyde from toluene may be higher in rats of night phase than those of day phase.

단백식이 조건을 달리하여 성장시킨 흰쥐에 Bromobenzene 투여가 간손상에 미치는 영향

신중규(Joong-Kyu Shin),채순님(Soon-Nim Chae),윤종국(Chong-Guk Yoon) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

식이성 단백질이 bromobenzene에 의한 간손상에 어떠한 영향을 미치는 지를 검토코저 흰쥐를 저(LP : casein 7%) 및 고(HP : casein 20%)단백식이로 1개월간 성장시킨 뒤 bromobenzene을 투여한 다음, 간무게, 혈청 alanineaminotransferase (ALT) 활성을 측정하여 간손상 정도를 두 군간에 비교하였다. 아울러 이에 대한 기전을 구명하는 일환으로 간조직 중 aniline hydroxylase (AH) 활성과 glutathione (CSH) 함량 및 이의 포합효소인 glutathione S-transferase (CST) 활성을 측정하였다. Bromobenzene 투여로 인한 대조군에 대한 간중량 및 혈청 ALT 활성 증가율은 LP군이 HP군 보다 높았다. 이때 간 AH 활성 증가율은 HP군 보다 LP군이 높았으나, 간 CSH 및 CST 활성은 LP군 보다 HP군이 높게 나타났다. 이상 실험결과를 종합하여 볼 때 bromobenzene에 의한 간독성을 식이 중 단백질 함량 감소에 영향을 받으며, 이는 LP군이 HP군 보다 bromobenzene 투여로 인한 간 AH 활성은 증가되나 bromobenzene 해독에 관여하는 간 glutathione 함량과 CSH 활성의 감소에 기인되기 때문인 것으로 생각된다. To evaluate an effect of dietary protein on the liver damage, the bromobenzene was intraperitoneally injected to the rats fed a low or high protein diet and then the liver weight per body weight and serum levels of alanine aminotransferase (ALT) activities were determined to demonstrate the differences in liver damage between the groups fed low or high protein diet. Hepatic aniline hydroxylase (AH), glutathione (CSH) content and glutathione S-transferase (CST) activity were also determined to clarify causes of liver damage between the two groups. Increases of liver weight per body weight and serum ALT activities were higher in bromobenzene treated rats fed low protein diet than those fed high protein diet. The increasing rate of hepatic AH activity was higher in bromobenzene-treated rats fed low protein diet than that in those fed high protein diet. Furthermore, hepatic glutathione contents and CST activities in bromobenzene-treated rats were higher in rats fed high protein diet than those fed low protein diet. In case of control group, the hepatic glutathione content and CST activity were also higher in rats fed high protein diet than those fed low protein diet.

랫드에 있어서 주야 시차가 Bromobenzene 대사에 미치는 영향

김광진,신중규,윤종국 한국독성학회 1997 Toxicological Research Vol.13 No.4

To investigate the circadian variation in the bromobenzene metabolism, bromobenzene(400 mg/kg body weight) was intraperitoneally administered to the rats every other day for 6 days both in the night; 24:00 and the day; 12:00. Each group of animals was sacrificed at 8hr after last injection of bromobenzene. The contents of hepatic CYP were more increased in control rats of night phase than those of day phase but in case of bromobenzene treatment there were no differences in hepatic CYP between rats of the night phase and those of day phase and the injection of prednisolon inhibited the hepatic CYP content in rats. Furthermore, the decreasing rate of hepatic glutathione contents to the control was higher in rats of day phase than those of night phase by the bromobenzene treatment. And the hepatic glutathione S-transferase activities were increased both in control and bromobenzene treated rats of the night phase than those of day phase. On the other hand, liver weight per body weight(%), hepatic lipid peroxide content, serum levels of alanine aminotransferase were more increased both in bromobenzene-treated and control rats of the night phase than those in the day phase. These results indicate that the rats of night phase may induce more accelerated formation of bromobenzene 3,4-oxide from bromobezene than those of day phase in rats.

치과기공사의 작업환경 개선 설비현황과 업무 스트레스 관련 연구

정인호,신중규,이송권 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

The purpose of this study was to find out a scheme to reduce a job stress of dental technicians and to increase and operation efficiency with the improvement of working environment. The method of study was by the questionnaire-survey from dental technicians working in the dental clinics or the dental department of clinics or hospitals in DaeguㆍKyungbuk, for the period of Jul 1 - Aug 2, 2002. Here 246 ones answered relative faithfully among 300 questionnaires were used. The results of study were as follows; 1. In the stress degree of dental technicians, stress by work load, job conflict, and pay showed significant results in crown jobs(p<0.001), long-time workers(p<0.0001) and discontinuing workers(p<0002), and technicians(p<0.007), respectively. 2. From looking over facility for improvement of working environment conditions of dental technicians, it's found that most of illumination and noise protection facilities and a part of respiratory protection facilities are not installed for dental technicians satisfactorily. 3. In relationship between installation for work environment improvement and stress, technicians who worked in good environment condition had lower stress degree than workers with unsatisfied work enrironment in most of all characteristics. Especially the result showed that installation for respiratory system is very important for reduction of factors causing stresses.