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장주은,김인정,김수형,신자민,금세진,김수린 한국생물공학회 2022 KSBB Journal Vol.37 No.2
Sargassum horneri is a sea-drifting brown macroalga often found along the coast of East Asian countries. It was recently found to be drifting from China toward Jeju Island in South Korea, causing damage to fisheries and vessels. Being considered as a marine waste, a huge amount of S. horneri was collected in the past 5 years, but an efficient and proper way to treat it has still not been found. Therefore, it is required to develop technologies to tackle this issue. Here, we conducted bioconversion of S. horneri by the yeast Saccharomyces cerevisiae in order to utilize it as a biomass source for producing ethanol. First, S. cerevisiae was engineered to extend its substrate range to mannitol, which is one of the major components of brown algae. Activation of the native HXT17 and MAN2 genes enabled the yeast to metabolize mannitol as the sole carbon source. Impact of pretreatment conditions, the type of hydrolytic enzymes, and biomass solid loadings on the ethanol production by the yeast were evaluated. The highest ethanol productivity was obtained when the biomass was pretreated at 121 o C and ethanol concentration was the highest when the biomass loading was 24% (w/v), giving the maximum concentrations of monosaccharides and ethanol of 47.29 g/ L and 22.94 g/L, respectively. The results obtained from this study suggest possible utilization of S. horneri as a raw material for cellulosic bioethanol production.
Nithnilanch Phachanseesoulath,김수형,신자민,박종범,Ryeongeun Kim,금세진,정덕열,김인정,김수린 한국식품저장유통학회 2022 한국식품저장유통학회지 Vol.29 No.4
Cassava is one of the most commonly imported raw materials for ethanol fermentation for the manufacture of food-grade distilled spirits in Korea. In cassava-producing countries, such as the Lao PDR, cassava can be considered low-price biomass for the production of bioethanol. In this study, the commercial wine yeast Saccharomyces cerevisiae EC1118 was tested for ethanol fermentation using cassava powder at a high solids loading (30%, w/v). α-Amylase and glucoamylase were used for the hydrolysis of cassava starch into glucose. To identify a suitable fermentation process for cassava, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) were compared. From the complete enzyme hydrolysis of cassava, 254.1 g/L of glucose was obtained. SSF showed higher ethanol titer during the first 12 h of fermentation, but SHF showed a higher ethanol titer after 24 h of fermentation. Finally, there was no significant difference between SHF and SSF in the final ethanol titer after 48 h fermentation (133.6 and 130.6 g/L, respectively). In summary, both SHF and SSF are applicable for ethanol production with high solid cassava using wine yeast EC1118 under the test conditions.