용해된 시멘트 분말이 붕어에 미치는 영향

신명자,이종은,서을원 한국환경생물학회 2015 환경생물 : 환경생물학회지 Vol.33 No.1

In this study, we studied the effect of dissolved cement powder on Carassius auratus byanalysis of the morphophysiological changes. The gill exposed to dissolved cement powder showedthe thickened primary lamellae and the activity of chloride cells and mucous cells was also significantlyincreased and the proliferation, separation and clubbing of gill filament was observed inthe secondary lamellae. In the kidney tissue, the space in Bowman’s capsule was widen and thearrangement of dermis was irregular due to the thinned epidermis in the integument tissue. Theactivities of antioxidant enzymes and LDH tended to increase with the duration of cement exposure. It was confirmed that the up-regulated proteins were identified as involved in glycolysis andenergy metabolism and down-regulated proteins were myofibrillar proteins which were involvedin muscle contraction by the cement exposure to the integument. With these results, dissolvedcement powder was thought to be a big threat to the survival of the fish because it causes the morphologicalchanges and weakens the physiological activity in C. auratus tissues.

뷰티산업과 의료관광산업의 연계 발전 방안 연구

신명자 한국메이크업디자인학회 2013 한국메이크업디자인학회지 Vol.9 No.1

배추흰나비 용기 특이 큐티클 단백질의 시공간적 역할

신명자,박정남,서을원,Shin, Myung-Ja,Park, Jeong-Nam,Seo, Eul-Won 한국생명과학회 2007 생명과학회지 Vol.17 No.2

배추흰나비 날개 형성기 동안 용기 특이 큐티클 단백질의 국소부위에 따른 분포와 신생조직합성에 있어 이의 시공간적 역할을 조사하였다. 큐티클에서 27 kd 단백질은 국소 부위에 따른 질적 변화는 거의 없으나 용 초이게 비교적 높은 농도를 보이다가 성충기로 감에 따라 서서히 농도가 낮아지는 양적인 변화를 나타내고 있다. 지방체에서 27 kd 단백질은 용화 후 3일까지는 흉부와 복부에서 전시기에 걸쳐 확인되고 있지만 그 이후 부터는 농도가 감소하는 경향을 나타내고 있다. Western blot 상에서도 27 kd 큐티클 단백질의 흉부와 복부의 국소적인 분포에는 거의 차이가 없으나 용말기와 성충 날개큐티클에서는 동질성을 보여주고 있다. $^{3}H-leucine$은 흉부와 복부 큐티클의 27 kd 단백질에 시공간적인 차이를 두고 통합되고 있으며, 용말기와 우화직전 흉부 큐티클과 복부 큐티클의 27 kd 단백질에서는 확인되지 않았으나 우화직전과 직후의 날개큐티클에 통합되는 패턴을 보여주고 있다. 지방체와 표피에서도 27 kd 단백질에 대한 통합이 두드러지게 나타나고 있다. 27 kd 용기 특이 큐티클 단백질은 국소 부위에 따른 질적, 양적 차이는 나타나지 않았으나 날개 형성기 동안 부분적으로 27 kd 단백질이 포함된 endocuticle이 소화, 재흡수 과정을 통해 성충 날개 큐티클의 형성에 관여할 것으로 생각된다. Present study aims to investigate the topical distribution of pupal stage specific cuticle protein and its temporal and spatial role during the wing formation of Artogeia rapae. ArCP27(27 kd cuticle protein) was identified as pupal stage specific cuticle protein in cuticle tissues and has not shown any qualitative differences by local portions of body. ArCP27 maintained constant concentration just after pupal ecdysis to 5-day old pupal stage but thereafter decreased. In fat body, ArCP27 was found in both thoracic and abdominal fat body from the last larval to pupal stage. In wing cuticle, ArCP27 began to find from 5-day old pupal stage. Immunologically ArCP27 in thoracic and abdominal cuticle has the response against the ArCP27 at 5-day old pupa but since then has no response. But the antibody against ArCP27 has reacted to 5- and 7-day old pupal and adult wing protein. $^3H-leucine$ was not incorporated into ArCP27 in 5- and 7-day old thoracic and abdominal cuticle but was incorporated into ArCP27 in 7-day old wing cuticle and adult wing, suggesting that ArCP27 partly participates the wing cuticle formation by the process of digestion and reabsorption of old cuticle.

Characterization and Distribution of Transferrin from the Last Larval Haemolymph of Papilio xuthus

신명자,김경근,김정숙,임재환,서을원,Shin, Myung-Ja,Kim, Kyoung-Keun,Kim, Jeong-Sook,Lim, Jae-Hwan,Seo, Eul-Won Korean Society of Life Science 2007 생명과학회지 Vol.17 No.8

Transferrin은 저장된 철분자를 운반하고 살아있는 유기체에서 철의 항상성을 유지한다. 호랑나비의 종령 유충 혈림프내 철 운반 단백질인 transferrin을 KBr 밀도구배 초원심분리와 Superose 6 HR을 이용한 fast protein liquid chromatography법으로 분리 정제하였으며, transferrin의 조직에 따른 분포는 면역화학적 방법에 따라 확인하였다. 정제된 transferrin의 아미노산 조성은 아스파르트산(Asp), 발린(Val), 루이신(Leu), 글루타민(GIu)이 많이 존재하였으며, subunit의 분자량은 78, 80 kDa으로 확인되었다. Immuno-diffusion을 통해 각 조직에서 분포를 확인한 결과 혈림프와 지방체에서 transferrin이 전시기에 걸쳐 뚜렷한 동질성을 나타냈다. 또한 rocket immuno-electrophresis법에 의해 transferrin의 양적 분포를 살펴보면 혈림프에서 용화 3일과 용화 5일에 증가하였으며, 지방체에서는 전용기와 용화직후에 양적인 증가를 나타냈다. Transferrin is a molecule carrying iron to store and maintain for iron homeostasis of living organisms. In this study, we have purified transferrin, as an iron-binding protein, from the last larval haemolymph of Papilio xuthus by KBr density gradient ultracentrifugation and gel filtration (superose 6 HR) using fast protein liquid chromatography (FPLC) and transferrin containing iron was identified by Ferene S staining. The purified haemolymph transferrin was shown to have molecular mass of 78 and 80 kDa and amino acid composition of transferrin was rich in aspartic acid, valine, leucine and glutamic acid. With immuno-diffusion assay, we confirmed the existence of the transferrin in the haemo-lymph and fat body by detection of visible and clear positive reaction. From the quantitative comparison by rocket immuno-electrophoresis process, the amount of transferrin were increased in the haemolymph of 3 days after pupation and the whole 5 days after pupation. Here, with biochemical and immunohistochemical analysis, we speculate the relationship of transferrin between the physical characteristics and distribution during metamorphosis of P. xuthus.

도시와 빈곤 100호를 축하하며

신명자 한국도시연구소 2012 도시와 빈곤 Vol.100 No.-

우리나라 뷰티산업의 현황과 향후 발전 방안에 관한 연구

신명자 한국메이크업디자인학회 2014 한국메이크업디자인학회지 Vol.10 No.2

This study was conducted in order to examine the current state of Korean beauty businesses and to assist in creating solutions for further development. Periodicals, books, news and internet data were compiled and analyzed to understand current beauty industry trends. Additionally, related research papers, reports from government were cited for this study. The outcome revealed that market conditions were improved in terms of quality and availability. However, the number of consumers who felt distressed about their physical and emotional state rose sharply. These consumers responded by investing in industries such as beauty and tourism. In terms of current trends, simple, holistic approaches were favored along with natural and organic products. In the domestic market, service industries including spa, leisure, and vacation are steadily developing as the demand for ‘healing’ and ‘detoxing’ is on the rise. Products and services that combine different elements such as beauty, health, medical, culture and tourism have been popular. Along with Korean dramas and K-pop, these packages have helped create a ‘Korean wave’ internationally, positively affecting the market. Make-up exportation has been increasing in neighboring Asian nations as demand in American and European markets has also grown. Cosmetic and beauty industries such as hair, make-up and nails are being integrated, with positive results, into other industries such as health, fashion, medical, cultural, leisure, and tourism. This pattern is giving rise to beauty lifestyle and ‘value-mixed’ industries. In conclusion, the beauty business can continue to expect promising growth in the future.

우리나라 화장 산업의 현황과 발전 방안에 관한 연구

신명자 한국메이크업디자인학회 2015 한국메이크업디자인학회지 Vol.11 No.2

This study is on the current status and focus of make–up industry in Korea. Today, technology of Korean beauty and make–up are receiving attention from all over the world. Regardless of age or gender, the amount used of cosmetics is steadily increasing due to the change of consumption trend attaching great importance to well-being. In accordance with the survey on the 1st quarter in 2015 conducted by STATISTICS KOREA, retail sales in domestic demand in cosmetic industry increased by 8.9% compared to the previous year. Economy downturn could not stop the prosperity of cosmetic industry, regardless of aftermath caused by global depression. Imitating celebrities caused by strong desire to look younger has been the craze for the past few years according to commercially used mass media. However, excessive stress and depression originating from natural aging is the evidence of the lack of philosophy on beauty and self-esteem. Recent study shows that educational curriculum on makeup and skin care generates positive impact on self-esteem and self-efficacy. In other words, appearance management has effective consequences. Therefore, it is needed to train health professionals regarding make-up, skin care, cosmetic products and aesthetic point of view. Categorized diagnosis and feedback by age on current status of each individual would be necessary as a part of customized education. Developing a program which can lead to qualitative change is expected to contribute to make-up industry itself.

佛畵에 대한 소고

신명자 중앙대학교 예술대학 회화학과 1978 卒業作品硏究報告書集 - 中央大學校 藝術大學 繪畵學科 Vol.1978 No.-

탁도 변화가 참갈겨니(Zacco roreanus)조직에 미치는 영향

신명자,김정숙,황윤희,이종은,서을원 한국하천호수학회 2008 생태와 환경 Vol.41 No.1

20대 여성의 미용행태별 건강위해 실태조사

신명자 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.1

With the increasing number of women involved in social activities, beauty industry has flourished. More women than ever before are concerned about make-up, hair care, skin care, and weight control, etc. With the success and commercialization of beauty industry, unconfirmed beauty care services have been introduced in the market. However, the health hazard from diverse beauty care services has not been systematically addressed. This study has the following two purposes: 1) to examine the utilization pattern of diverse beauty care services; and 2) to examine the health hazard from beauty care services. For this study, two major universities located in Seoul area were selected. While 450 female college students were selected from one women's university, the other 450 were from mixed university. Using survey questionnaire, we interviewed with 900 female college students. The results are as follows: As for the beauty care patterns, makeup accounted for 78.6%, Skin care 49.5%, weight/body control 32.0%, hair care 81.5%, and cosmetic operation 14.9%, indicating significant high rates of makeup(x2=9.215, p=0.002) and hair care(x2=4.435, p=0.035) at both mixed and women's universities. The health hazard was measured using four questions. The past studies have not paid special attention to the methodological issue of how to ask health hazard. Depending on how to ask health hazard, the percentage of respondents reporting health problems varied. When the respondents were asked whether or not they had suffered health problems for a short period, over 60% of respondents reported health problems; when they were asked in other ways-1) whether or not they had suffered health problems for a long period, 2) whether or not they had suffered whole body problems, 3) whether or not they had permanent traces on skin due to beauty care activities-, the percentage of respondents reporting health problems dropped substantially to less than 10%, This finding indicates the importance of 'words' used in questions. The relationships between health problems and utilization of beauty care services were examined, using χ2 test or t test. We found that there was a significant relationship between health problem and beauty care.