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가두리 양식장의 Vibrio vulnificus 검출 및 제어 방법
성치남,송계민,이규호,양성렬 한국미생물학회 2002 미생물학회지 Vol.38 No.4
2000년 1 월부터 2000년 10월까지 가두리 양식장에서 Vibrio vulnificus를 검출하였고 이들의 억제 방법을 연구하였다. 이 세균의 검출은 선택적 분리법과 vvhA 유전자를 확인하는 방법을 이용하였다. V. vulnificus는 수온이 $17^{\circ}C$이상인 5월부터 검출되었고 $19^{\circ}C$ 이상인 6월부터 9월까지는 대부분의 시료에서 검출되었다. V. vulnificus를 제어하기 위한 방법 중 냉동 및 냉장 처리는 살균효과를 나타내지 못했다. Citric acid도 균의 생장을 억제하지 못했으나, 500 mg/1이상의 EDTA가 첨가될 경우 균이 완전히 사멸되었다. 분말 광촉매인 산화티타니움은 자외선을 조사할 경우 15분~1 시간 이내에 이 세균을 완전히 사멸시키는 효과를 나타내었다. 산화티타니움을 유리 구슬에 코팅한 광촉매 장치를 이용하여 0.2/min의 turnover rate로 사멸효과를 얻었다. Detection of Vibrio vulnificus in fish farm and searching for the bactericidal methods on this bacteria were studied. To detect this microorganism in sea water, mud, fish and mussels, selective isolation methods and detection of vvhA gene were used from January to October,2000. V. vulnificus was detected from May when the water temperature was over $17^{\circ}C$. From June to September, higher than $19^{\circ}C$, this bacteria could be isolated from most of the samples. Freezing and refrigerating did not inhibit the growth of V. vulnificus. Citric acid did not show the bactericidal effect, but more than 500 mg/l of EDTA did. With the aid of UV and photocatalyst, $TiO_{2}$ showed bactericidal effect after 15 minute treatment. Photocatalytic system consisted of glass bead coated with $TiO_{2}$ and UV illumination showed bactericidal effect on V. vulnificus at the turnover rate of 0.2/min.
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혈액에서 Sucrose 양성 Vibrio vulnificus 분리 1예
김신무 ( Shin Moo Kim ),송계민 ( Kye Min Song ),김승아 ( Seung A Kim ),최수연 ( Su Youn Choi ),임효빈 ( Hyo Bin Im ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.2
Vibrio vulnificus is a halophilic bacterium frequently involved in human infection of seafood-associated primary septicemia and primary wound infection, mostly in men with over 40-years of age with underlying liver disease. The primary septicemia, which is the most common form of V. vulnificus infection in Korea, is defined as a systemic illness presenting fever or hypotension with recovery of V. vulnificus from blood or tissue without the apparent primary focus of infection. V. vulnificus typically do not produce acid from sucrose, but a case of primary septisemia was found in a patient at Chonnam K hospital in 1993 from whose blood a sucrose-fermenting strain was isolated. The patient was a 62-year-old man, heavy drinker, with underlying liver disease. He consumed a raw seafood dish two days before onset of the present illness. His symptoms were tenderness and swelling on the right foot. He rapidly developed septicemia, resulting in sudden death. V. vulnificus was isolated from the venous blood culture of the patient. On subculture, the isolate formed yellow colonies on TCBS and produced acid from sucrose. Because of these characteristics, species identification was not achieved by the API 20E and was delayed. Other characteristics of the isolate were identical to those of typical V. vulnificus. The isolate was common serotype O4A and possession of V. vulnificus-specific cytolysin gene was detected by PCR. The isolate was susceptible to all the antimicrobial agents tested including tetracycline, but was intermediate to colistin. In conclusion, it is important that microbiologists be aware of the presence of sucrose-positive V. vulnificus when he or she identifies gram-negative bacilli, which is isolated from the blood of patients with a recent history of raw seafood dish consumption.
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HeJicobacter pyJori의 신속한 진단을 위한 연구
김양호 ( Yang Ho Kim ),신명근 ( Myuong Geun Shin ),백근식 ( Keun Shik Baik ),송계민 ( Kye Min Song ),임준영,성치남 ( Chi Nam Seong ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Optimal conditions for the rapid diagnosis of Helicobacter pνlori were determined. Bacterial strains were isolated from biopsy where the u1cer was observed with endoscope. Morphological and cultural properties of the isolates were determined. Efficiency and srnsitivity of several diagnostic tests were compared. U1cer tissues as well as pure cultures of H. pνlori showed positive reaction within 5 min in urease test broth or semi-solid agar containing 10% urea and 0.1% phenol red. Urease test was highly reproducible and sensitive. Urease A gene extracted from pure culture and tissue was amplified by nested PCR using HEPY 1/2 and HEPY3 / 4 primers. Electrophoresis of PCR product confirmed that DNAs extracted from tissues and isolates which show positive reaction in urease test contain Urease A gene. Among several diagnostic methods used in general, urease test was recommended as a convenient, cheap and easy method.
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Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교
김신무(Shin-Moo Kim),소향아(Hyang-Ah So),송계민(Kye-Min Song),이영엽(Young-Youp Lee),임채원(Chae-Won Lim),이재형(Jae-Hyung Lee),소홍섭(Hong-Seob So),김진경(Jin-Kyung Kim),박래길(Rae-kil Park),박석돈(Seok-Don Park) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.4
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Vibrio vulnificus의 생물형, 혈청형, Repetitive Sequence-Based PCR 양상 및 항균제 감수성 시험
김신무 ( Shin Moo Kim ),소향아 ( Hyang Ah So ),최수연 ( Su Youn Choi ),심은숙 ( Eun Sook Shim ),송계민 ( Kye Min Song ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
This study was performed to provide c1arity on the environmental distribution of Vibrio vulnificus oysters, biological characteristics, distribution of biotypes and serotypes, the detection of the cytolysin gene by PCR, repetitive sequence-based PCR (rep-PCR) analysis and antimicrobial susceptibility of the isolates from oysters and patients in Korea. ν vulnificus was isolated from 67 (38.5%) of the 175 samples of oysters collected at a shoreline in the Kunsan city area in July and August, 2002. Oyster and patient isolates had similar biochemical characteristics, i.e., all were positive on an ONPG test, but not for sucrose. When comparing the identification of V. vulnificus obtained with the API 20E assay and serotyping, 87 API 20E profiles with both a separation test on the colony of TCBS agar and an ONPG test were obtained with 84 profiles (96.6%), but three isolates were not identifiable with the API 20E system. API 20E code numbers showed 13 different biotypes in 87 isolates. The lrighest rate of 48 (55.2%) isolates of API 20E code numbers was obtained with code No. 5146105 (biotype N). Oyster is이ates belonged to tlrirteen serotypes and patient strains belonged to 9 serotypes. Prevalent serotypes were 012, and 04A, 16 (18.6%) and 13 (15.1 %) from oysters, and 52 (67.5%) and 7 (9.1%) of 04A and 04AB from patients, respectively. 163 isolates of V. vulnificus cytolysin gene from oysters and patients were positive by PCR. 30 V. vulnificus isolates were divided into 5 genotypes using rep-PCR analysis. Only three bands have all of the isolates from the oysters by rep-PCR. on an antimicrobial susceptibility test they were susceptible to tetracyc1ine, chloramphenicol and ciprofloxacin, but none were susceptible to colistin.
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