http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Vibrio vulnificus의 생물형, 혈청형, Repetitive Sequence-Based PCR 양상 및 항균제 감수성 시험
김신무 ( Shin Moo Kim ),소향아 ( Hyang Ah So ),최수연 ( Su Youn Choi ),심은숙 ( Eun Sook Shim ),송계민 ( Kye Min Song ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
This study was performed to provide c1arity on the environmental distribution of Vibrio vulnificus oysters, biological characteristics, distribution of biotypes and serotypes, the detection of the cytolysin gene by PCR, repetitive sequence-based PCR (rep-PCR) analysis and antimicrobial susceptibility of the isolates from oysters and patients in Korea. ν vulnificus was isolated from 67 (38.5%) of the 175 samples of oysters collected at a shoreline in the Kunsan city area in July and August, 2002. Oyster and patient isolates had similar biochemical characteristics, i.e., all were positive on an ONPG test, but not for sucrose. When comparing the identification of V. vulnificus obtained with the API 20E assay and serotyping, 87 API 20E profiles with both a separation test on the colony of TCBS agar and an ONPG test were obtained with 84 profiles (96.6%), but three isolates were not identifiable with the API 20E system. API 20E code numbers showed 13 different biotypes in 87 isolates. The lrighest rate of 48 (55.2%) isolates of API 20E code numbers was obtained with code No. 5146105 (biotype N). Oyster is이ates belonged to tlrirteen serotypes and patient strains belonged to 9 serotypes. Prevalent serotypes were 012, and 04A, 16 (18.6%) and 13 (15.1 %) from oysters, and 52 (67.5%) and 7 (9.1%) of 04A and 04AB from patients, respectively. 163 isolates of V. vulnificus cytolysin gene from oysters and patients were positive by PCR. 30 V. vulnificus isolates were divided into 5 genotypes using rep-PCR analysis. Only three bands have all of the isolates from the oysters by rep-PCR. on an antimicrobial susceptibility test they were susceptible to tetracyc1ine, chloramphenicol and ciprofloxacin, but none were susceptible to colistin.
Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교
김신무(Shin-Moo Kim),소향아(Hyang-Ah So),송계민(Kye-Min Song),이영엽(Young-Youp Lee),임채원(Chae-Won Lim),이재형(Jae-Hyung Lee),소홍섭(Hong-Seob So),김진경(Jin-Kyung Kim),박래길(Rae-kil Park),박석돈(Seok-Don Park) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.4
환아의 혈액과 변에서 분리된 Shigella flexneri의 생화학적 성상, 항균제 감수성 및 Pulsed-Field Gel Electrophoresis 분석
김신무 ( Shin Moo Kim ),임채원 ( Chae Won Lim ),소향아 ( Hyang Ah So ),심은숙 ( Eum Sook Shim ),김은숙 ( Eun Sook Kim ),이규식 ( Kyu Sik Lee ),정윤섭 ( Yunsop Chong ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3
Shigellosis is the most common bacterial gastroenteritis both in developing and developed countries, but bacteremia due to Shigella spp. is very rare. In developed countries recent shigellosis is mostly caused by S. sonnei, but S. flexeri infection is rare. We had rare cases of S. flexeri infections in a family in the Jeonbuk Province: an 8-year-old boy with bacteremic shigellosis and 10- and 12-year-old brothers with diarrhea. The isolates had identical biochemical characteristics, and were resistant to ampicillin, chloramphenicol, and co-trimoxazole. PFGE pattern of NotⅠ-restricted genomic DNA suggested that the isolate from blood was closely related to the two strains isolated from stool which had an identical PFGE pattern.
임상 검체에서 분리된 Bacillus cereus 의 성상, 장독소 생성 및 항균제 감수성
김신무 ( Shin Moo Kim ),김은철 ( Eun Cheol Kim ),소향아 ( Hyang Ah So ),이규식 ( Gyu Sik Lee ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1
Biochemical characteristics, enterotoxin production and antimicrobial susceptibility were determined for 30 strains of Bacillus cereus isolated from stool specimens of diarrhea patients at an university hospital in Chulabuk-do province. Positive rate for VP reaction and citrate utilization were lower, (33 % and 40 % respectively) while the rates of acid production from mannitol, arabinose, and xylose were higher (17 %, 13 % and 3 % respectively) than those obtained by other investigators. The enterotoxin gene was detected in 18 of 30 isolates (60 %) by PCR, and the toxin was detected from all of the toxin gene-positive isolates by RPLA test. The agar dilution test showed that all isolates were resistant to penicillin G and 73 % were to cephalothin, but all were susceptible to ciprofloxacin, clindamycin, erythromycin, fusidic acid, gentamicin, rifampin, teracycline and vancomycin. We conclude that B. cereus isolates producing acid from mannitol, arabinose and xylose exist, that PCR can be used to detect enterotoxin genes rapidly and accurately, and that this organism is susceptible to various antimicrobial agents though not penicillin G and cephalothin.
엄상검체에서 분리한 Vibrio parahaemolyticus의 생 물학적성상, 혈청형, toxR 유전자의 검출 및 시험관내 항균제 감수성
김신무 ( Shin Moo Kim ),송남경 ( Nam Kyung Song ),정재옥 ( Jae Ok Chung ),신상희 ( Sang Hee Shin ),소향아 ( Hyang Ah So ),이규식 ( Gyu Sik Lee ),정윤섭 ( Yun Sop Chong ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Vibrio parahaemolyticus is one of the most important food-bome pathogens in Korea, Japan, and other cOlllltries with long coast1ines. The aim of this study was to determine the biochemical characteristics, serovar, antimicrobial, susceptibi1ity and possession of toxR gene of the 46 V. parahaemolyticus isolates from c1inical specimens. Biochemical characteristics of the these were typical, but 28 % and 4 % of isolates showed negative to Simmon citrate and omithine decarboxylase test, respectively. Six kinds of serovars were detected in the isolates. Serovar 03:K6 were most prevalent i.e., 85%. Other serovar inc1uding 03:K9, 03:K55, 03:K56, 03:KUT and R were veη rare. Most of the isolates were resistant to ampici11in, carbenici11in and cefazolin, but none were resistant to most of the other antimicrobial agents including tetracyc1ine, which is the drug of choice for the treatment of Vibrio infection. V. parahaemolyticus toxR gene was detected from all of the 46 isolates. It is conc1uded that the biochemical characteristics of most of the V. parahaemolyticlα of isolated Wonkwang University Hospital are typical, that prevalent serovar are 03:K6, and that isolates with antimicrobial resistance with the exception ampicillin, carbenicillin 뻐d cefazolin are very rare. Also toxR gene detection by the PCR test can be used for rapid identification of the V. parahaemolyticus in the c1inical laboratory.