http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
한우에 있어서 발정주기중 혈중 Progesterone 및 Insulin - like Growth Factor-I 농도의 변화
성환후,우제석,임석기,고응규,백광수,박진기,구용범,이장형 ( H . H . Seong,J . S . Woo,S . K . Im,Y . G . Ko,K . S . Baek,J . K . Park,Y . B . Koo,J . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
The objective of this study was to determine changes in circulating progesterone and insulinlike growth factor-I(IGF-I) concentrations during estrous cycles in Hanwoo. The serum progesterone and IGF-I concentrations during the estrous cycles in the apparently healthy normal cycles and freemartins of 670 days old were measured with a commercial Radio Immuno Assay(RIA) kit. Serum progesterone concentration was low from Day 1 to 3(about 0.25ng/㎖) and then increased gradually to 9.3ng/㎖ by Day 17, and decreased dramatically by Day 21 of the estrous cycle. However, serum progesterone concentration of freemartins was almost undetectable during the same periods of normal Hanwoo. Serum IGF-I concentrations from Day 3 to 16 of normal estrous cycle were higher than those of early luteal stage( Day 1∼2) and luteal regression stage (Day 19∼20). The lowest serum IGF-I concentration was observed on Day 21. In the freemartin of Hanwoo, serum IGF-I concentrations were 1/4 of those in the normal cycles. It is suggested that the serum progesterone concentration is closely related to the serum IGF-I concentration in Hanwoo.
Anti-Inhibin Serum 및 Bovine Follicular Fluld 처리가 한우 난소의 난포 발달과 혈중 호르몬 변화에 미치는 영향
성환후,양병철,황성수,박성재,고응규,노환국,양보석,Seong, H.H.,Yang, B.C.,Hwang, S.S.,Park, S.J.,Ko, Y.G.,Rho, W.K.,Yang, B.S. 한국수정란이식학회 2006 한국동물생명공학회지 Vol.21 No.4
본 연구는 한우 난소의 난포 발달에 있어서 bFF 또는 anti-inhibin serum(AI)의 생리적 역할을 검토하기 위해 수행하였다. Saline(0.95%, control), bFF 또는 AI를 경정맥 주사 처리한 9마리의 한우 암소에서 채혈하여 혈중 estradiol-17$\beta$(E2), inhibin 및 progesterone(P4) 농도를 분석하였으며, 이들 처리에 의한 난포의 발달은 초음파 진단기를 이용하여 관찰하였다. 본 연구에서 얻어진 결과는 다음과 같다. 혈중 inhibin 농도는 bFF 처리구에서 배란 후 102시 간부터 유의적으로 증가하여(p<0.05) 지속적으로 높은 수준을 유지하였다. 혈중 E2 농도는 saline 처리구의 경우 bFF 처리구와 비슷한 수준으로 배란 후부터 낮은 농도를 유지하였으나, AI 처리구는 배란 후 36시간 이후부터 108시간까지 유의적으로 높은 수준을 유지하다가(p<0.05) 점차적으로 감소되는 경향을 나타내었다. 혈중 P4 농도는 saline 처리구와 AI 처리구에서 배란 후 68시간째부터 증가하기 시작하였으나, bFF 처리구에서는 배란 후 84시간부터 180시간까지 saline 또는 AI 처리구에 비해 유의적으로 낮은 농도를 나타내었다(p<0.05). 한편, AI 또는 bFF 처리가 난포 발달에 미치는 영향을 알아보기 위하여, 초음파 진단기를 이용하여 난소의 난포 발달을 조사한 결과, bFF 처리구에 비해 AI 처리구에서 발달 난포가 많은 것을 확인할 수 있었다. 이상의 결과를 종합해 보면, 외인성 AI는 한우난소에서 분비하는 inhibin을 특이하게 억제하여, inhibin에 의해 분비가 억제되어 있던 FSH 분비를 촉진시킴으로써 난포 발달과 estrogen의 분비 증가를 유발하는 것으로 사료된다 따라서 한우에 있어서 배란 직후 새롭게 발달하는 난포에는 적어도 inhibin이 관여하고 있는 것으로 사료된다. This study was performed to investigate the physiological effects of bovine follicular fluid (bFF) or anti-inhibin serum (AI) on follicular development in Hanwoo. Saline (0.95%), bFF or AI (total of 40 ml) were administered into the jugular vein in 9 Hanwoo cows. The plasma inhibin, estradiol-17 $\beta$ (E2), and progesterone (P4) levels were measured using RIA or ELISA kit and the number of ovarian follicles was observed by ultrasonography at 72 hr after ovulation. The plasma inhibin level in bFF treatment group was significantly increased and maintained higher level from 102 hr after ovulation compared to that of saline and AI groups (p<0.05). In plasma E2 level, AI treatment group showed significantly higher level from 36 hr to 108 hr after ovulation than that of saline and bFF groups (p<0.05). After that it showed decreasing tendency. The plasma P4 level was increased in control and AI treatment groups at 68 hr after ovulation. However, it was maintained significantly lower level in bFF group from 84 hr to 180 hr compared to that of saline and AI group (p<0.05). As a result of ultrasonography at 72 hr after ovulation, higher number of follicles was shown in AI treatment group compared to bFF groups, although the difference was not statistically significant. Taken together, it can be postulated that a treatment of synthesized AI inhibits the secretion of inhibin, stimulates FSH secretion inhibited by inhibin, and induces follicular development and estrogen secretion. According to these results, a development of ovarian follicle immediately after ovulation is associated closely with inhibin in Hanwoo heifers.
성환후,이장형,Seong, H.H.,Lee, J.H. 국립한국농수산대학교 교육개발센터 2002 현장농업연구지 = Journal of practical agricultural resear Vol.4 No.1
This study was conducted to investigate the physiology of optimal reproductive age in Hanwoo for protection to decrease of reproductivity and improvement of production of offspring. Thirty two cows were devided into 4 groups of treatments : T1(12 months of age and 0.5kg daily gain), T2(12 months of age and 0.8kg daily gain), T3(15 months of age and 0.5kg daily gain) and T4(18 months of age and 0.5kg daily gain). The days of the first heat of treated cows were 263.3±6.4 days and average weight was 181.1±11.3kg. The conception rates of first insemination were 25%(T1) and 75%(T4), and the number of insemination of T3 and T4(both 1.5) was lower than those of T1 and T2(2.3 and 2.4), respectively. With regard to estrus return after the first parturition, T1, T2, T3 and T4 showed 66.2,76.7, 62.4 and 68.5 days, respectively, indicating the average days of estrus return was 65.7. Plasma progesterone(P4) concentration was nearly the same during the observation periods of treated cows and P4 began to be detected after 12months. Only 5(15.6%) out of 32cows showed normal estrus cycle and ovulation before 12 months. During the peri-parturition period, P4 concentration was rapidly decreased and there was no detection of P4 from parturition to 40 days after milking. P4 would be released again on 45 day after parturition. The results imply that the optimal reproductive age of Hanwoo heifers would be around at the 14 months of age, 110cm height and 265kg weight.
흰쥐 분만의 인위적 조절에 관한 연구 4 . Aspirin 투여에 의한 분만지연 시험
윤창현(C . H . Yun),성환후(H . H . Seong),장규태(K . T . Chang) 한국축산학회 1988 한국축산학회지 Vol.30 No.2
This experiment was carried out to study efficacy of the exogenous Aspirin for prolongation of parturition in white rats. Sixty of the pregnant White rats used were injected with Aspirin. The survival rate of newborn, the administration time and the amount of dosage affecting on the prolongation of parturition were investigated. The results obtained were as follows: 1. The average survival rate of newborns in groups injected with Aspirin on day 15, 17 and 19 of gestation were 77.1%, 76.0% and 79.7% respectively. There were no differences by the amount of dosage of Aspirin. 2. Mean gestation length of rats injected Aspirin on day 15, 17 and 19 of gestation were 23.08±.22, 22.65±0.12 and 23.18±0.30 days, respectively. There were no significant differences (P$lt;0.05) among experimental groups. 3. For the amount of dosage of 50㎎/㎏ × ltime, 100㎎/㎏ × Mime and 150㎎/㎏ × Mime per ㎏ per day the average intervals from injection to parturition were 22.67±0.17, 22.97±0.25 and 23.28±0.22 days, respectively. There were no significant differences (P$lt;0.05) among experimental groups.
흰쥐 분만의 인위적 조절에 관한 연구 3 . Indomethacin 투여에 의한 분만지연 시험
윤창현(C . H . Yun),성환후(H . H . Seong),장규태(K . T . Chang) 한국축산학회 1987 한국축산학회지 Vol.29 No.11
This experiment was carried out to study the efficacy of the exogenous indomethacin for prolongation of parturition in white rats. Sixty of the pregnant white rats used were injected with indomethacin. The survival rate of newborn, the administration time and the amount of dosage affecting on the prolongation parturition were investigated. The results obtained were as follows: 1. The average survival rates of newborn in groups injected with indomethacin on day 15, 17 and 19 of gestation were 78.2, 82.0 and 85.5%, respectively. There were no differences by the amount of dosage of indomethacin. 2. Mean gestation lengths of rats injected indomethacin on day 15, 17 and 19 of gestation were 23.39±0.18, 23.28±0.22 and 23.19±0.09 days, respectively. There were no significant differences (P$lt;0.05) among experimental groups. 3. For the amounts of dosage of 0.2㎎ × 1time, 0.4㎎ × 1time and 0.6㎎ × 1time per ㎏ per day the average intervals from injection to parturition were 22.98±0.05, 23.48±0.15 and 23.40±0.04 days, respectively. There were no significant differences (P$lt;0.05) among experimental groups.
PCR 기법을 이용한 소 Y- 염색체 특이적 DNA 단편 증폭에 의한 성판별 연구
오성종(S . J . Oh),이창수(C . S . Lee),양보석(B . S . Yang),정진관(J . K . Jung),성환후(H . H . Seong),임경순(K . S . Im) 한국축산학회 1993 한국축산학회지 Vol.35 No.5
This experiment was conducted to indentify the sex of cattle using PCR. Bovine Y-specific and bovine specific sequences as primer were synthesised and genomic DNA was extracted from blood of Korean Native cattle and Holstein. When the bovine specific sequences were added to the reaction buffer as primer, both male and female genomic DNAs were amplified .and formed bands(2166p) after the electrophoresis. However, when the Y-specific sequences were added, only male genomic DNA was amplified and formed bands(141hp). PCR products did not increase when the amount of bovine specific DNA as primer was more than 50ng. Two distinct DNA fragments were detected when amplified by two-step PCR method(that is, amplification with bovine specific DNA for 30 cycles after the ampilfication with Y-specific DNA for 30 cycles).
한우에 있어서 Rosette Inhibition Test 에 의한 조기 임신인자(Early Pregnancy Factor)의 검출
성환후,백광수,고응규,신기준,박용윤,신원집 한국축산학회 1997 한국축산학회지 Vol.39 No.4
Early pregnancy factor(EPF) is one of the pregnancy associated proteins, and it is detected in senun of many pregnant animals shortly after fertilization. The present study was conducted to establish a system of early pregnancy by EPF detection in Hanwoo cows. The EPF activity was measured by the rosette inhibition test on day 15 after artificial insemination or non-insemination. The rate of rosette formation between Hanwoo lymphocytes and sheep red blood cells was significantly(P$lt;0.05) higher compared to that between Hanwoo lymphocytes and goat, or rabbit red blood cells. The rosette formation between lymphocytes suspension and sheep red blood cells with anti-lymphocytes was not significantly(P$lt;0.05) changed until 5∼20 min. of culture, and then decreased dramatically by 30 min. There were significant(P$lt;0.05) differences in the rosette inhibition titer(RIT) between pregnant and non-pregnant Hanwoos on day 15 after artificial insemination. The results indicate that the expression of early pregnancy factor by the rosette inhibition test might be used to diagnosis of early pregnancy in Hanwoo.