신성 고혈압 백서 심방의 심방이뇨 호르몬분비 특성

조경우,설경환,김선희,설경미,고규영,Cho, Kyung-Woo,Seul, Kyung-Hwan,Kim, Suhn-Hee,Seul, Kyung-Mee,Koh, Gou-Young 대한생리학회 1990 대한생리학회지 Vol.24 No.2

It has long been suggested that the cardiac atrium is a low pressure volume receptor controlling body fluid volume and blood pressure. Recently, the cardiac atrium has been found to contain a family of powerful peptides. To clarify the relationship between high blood pressure and the biologically active atrial peptides, experiments were done to define the characteristics of atrial natriuretic peptide secretion in the isolated perfused atria of renal hypertensive rats. Higher concentrations of plasma atrial natriuretic peptide and renin activity were observed in the two-kidney, one clip hypertensive rat compared to the normotensive rat. Atrial volume changes in response to pressure elevations were attenuated in hypertensive rats compared to normotensive rats. Incremental response to atrial volume changes in ANP secretion was accentuated in hypertensive rats. These date suggest that the accentuated atrial natriuretic peptide response to volume changes of hypertensive rats may be a physiological or pathphysiological adaptation to the high blood pressure and may be, at least in part, responsible for the elevated levels of plasma atrial natriuretic peptide observed in hypertensive rats.

Changes in Cardiovascular and Renal Functions by Temperature and Epinephrine in the Freshwater Turtle, Amyda Japonica

김선희,조경우,설경환,고규영,Kim, Suhn-Hee,Cho, Kyung-Woo,Seul, Kyung-Hwan,Koh, Gou-Young The Korean Physiological Society 1987 대한생리학회지 Vol.21 No.2

수온변화에 따른 심맥관계 및 신장기능의 변화와 생체실험을 통한 온도에 의한 adrenoceptor의 변형을 알아보기 위해, 무마취 자라에서 $18^{\circ}C$에서 $25^{\circ}C$로 수온을 증가시 나타나는 혈압, 심박동수 및 신장기능의 변화를 관찰하고, epinephrine 1 ug/kg과 10 ug/kg을 상이한 온도에 노출된 자라의 정맥내 투여하여 나타나는 효과를 비교하였다. 1) $18^{\circ}C$에서 $25^{\circ}C$로 수온을 증가시킴에 따라 심박동수는 현저히 증가하여 일정하게 유지되었으나, 혈압 및 혈장 renin 활성도는 변화하지 않았다. 온도증가에 의해 뇨량, 사구체여과율 및 전해질 배설량의 현저한 증가를 보였으나 90분부터는 서서히 감소하기 시작하였다. 2) 수온 $18^{\circ}C$에 노출된 자라에서 epinephrine은 dose-dependent한 양상으로 혈압 및 심박동수를 증가시켰으며, 다량의 epinephrine 투여시 작용시간은 현저히 연장되어 있었다. $25^{\circ}C$에 노출된 자라에서는 epinephrine에 의한 혈압상승 효과 및 심박동수 증가는 나타났으나, dose dependency나 작용시간의 차이는 발견할 수 없었다. 3) 동량의 epinephrine에 의한 혈압 및 심박동수의 증가효과는 $18^{\circ}C$와 $25^{\circ}C$에 노출된 자라에서 유의한 차이를 발견할 수 없었으나, $18^{\circ}C$에 노출된 자라에서 epinephrine의 작용시간 및 반감기가 현저히 연장되어 있었다. 4) Epinephrine 투여에 의해 뇨량, 사구체여과율 및 전해질 배설량의 증가를 관찰하였으며, 이는 dose-dependent 양상이었다. 그러나, 신장효과의 유의한 차이는 상이한 온도에 노출된 두 군에서 발견할 수 없었다. 이상의 결과로, 온도증가에 의한 이뇨 및 sodium 배설효과는 혈관이완에 의한 사구체여과율의 증가에 기인한 것으로 사료되며, 상이한 온도에 노출된 자라에서 epinephrine 효과의 차이를 발견할 수 없었던 것은 본 실험에서 가한 좁은 범위의 온도의 변화 내에서는 adrenoceptor의 변형이 나타나지 않을 것이라고 추론하였다. 그러나 저온에서의 epinephrine의 작용시간의 연장은 아마도 epinephrine의 파괴 효소의 활성도의 감소인 것으로 사료된다.

자라 신장기능에 미치는 Atrial Natriuretic Peptide의 효과

조경우,김선희,고규영,설경환,Cho, Kyung-Woo,Kim, Suhn-Hee,Koh, Gou-Young,Seul, Kyung-Hwan 대한생리학회 1987 대한생리학회지 Vol.21 No.1

Effects of synthetic atrial natriuretic peptide and furosemide on the cardiovascular and renal functions were examined in the freshwater turtle, Amyda japonica. Both atria and ventricle of turtle contained an immunoreactive atrial natriuretic peptide. Synthetic rat atrial natriuretic peptide (atriopeptin III) and turtle atrial extract caused a decrease in mean arterial blood pressure and the vasodepressor effect was dose-dependent. In hydrated turtles received either atriopeptin III or turtle atrial extract, no significant change in renal function was observed until 100 min except a slight natriuresis at 60 or 100 min after injection of 30 ug/kg atriopeptin III or atrial extract, respectively. However, furosemide, 2 mg/kg, caused marked diuresis, natriuresis and kaliuresis. In non-hydrated turtles, no significant change in renal function was observed until 6 hrs following injection of 30 ug/kg atriopeptin III. Plasma aldosterone decreased at 2 hr and increased at 24 hr after injection of atriopeptin III although plasma renin concentration did not change. But, furosemide caused persistent diuresis, natriuresis and kaliuresis. Additionally, plasma aldosterone and renin concentrations were significantly increased at 24 hrs after injection of furosemide. In conclusion, we suggest that the freshwater turtle may have an atrial natriuretic peptide in heart and vascular receptors for atrial natriuretic peptide, and that atrial natriuretic peptide is more important in the regulation of blood pressure rather than that of renal function in freshwater turtles. We also suggest that an increased plasma renin concentration caused by furosemide may not be due to the sodium concentration delivered to macula densa, but due to the dehydration caused by persistent diuresis and natriuresis.

신성 고혈압 백서에서 Atrial Natriuretic Peptide의 신장기능에 미치는 효과

조경우,김선희,소준노,류훈,설경환,Cho, Kyung-Woo,Kim, Suhn-Hee,So, June-No,Ryu, Hoon,Seul, Kyung-Hwan 대한생리학회 1989 대한생리학회지 Vol.23 No.1

Since the atrial receptor was suggested to be involved in the control of extracellular fluid volume, it has been shown that the granularity of atrial cardiocytes can be changed by water and salt depletion, and that an extract of atrial tissue, when injected intravenously into anesthetized rats, causes a large and rapid increase in renal excretions of sodium and water. The immunoreactive atrial natriuretic peptide (ANP) has been found in the plasma of patients suffering from various cardiovascular diseases. A high level of ANP in the plasma has been reported in essential hypertension. Several studies on the effects of ANP on renal function and arterial blood pressure have presented contradictory results showing attenuated or accentuated responses. Thus, involvement of the ANP in the development of hypertension remains unresolved. Present study was undertaken to investigate whether the ANP is involved in the development of hypertension in two-kidney one-clip Goldblatt hypertensive rats. The plasma concentration of immunoreactive ANP appeared to be significantly elevated in hypertensive rats as compared with normotensive Goldblatt operated and sham-operated rats. Plasma renin concentration was higher in hypertensive rats than in normotensive rats, as observed in earlier experiments. Intravenous infusions of ANP resulted in increases of urine flow and urinary excretions of sodium and potassium in both hypertensive and normotensive rats. The renal response to ANP was markedly accentuated in Goldblatt hypertensive rats. The plasma concentration of ANP showed a linear relationship with the arterial blood pressure. Infusions of ANP reduced blood pressure both in hypertensive and normotensive rats. These results suggest that in Goldblatt hypertensive rats an elevation of ANP level in the plasma may not be a cause, but instead a consequence of hypertension, and that the renal responsiveness to the ANP is accentuated by some unknown mechanisms.

수온변화와 Epinephrine에 의한 자라의 심맥관계 및 신장기능의 변화

김선희(Kim, Suhn-Hee),조경우(Cho, Kyung-Woo),설경환(Seul, Kyung-Hwan),고규영(Koh, Gou-Young) 대한생리학회 1987 대한생리학회지 Vol.21 No.2

수온변화에 따른 심맥관계 및 신장기능의 변화와 생체실험을 통한 온도에 의한 adrenoceptor의 변형을 알아보기 위해, 무마취 자라에서 18℃에서 25℃로 수온을 증가시 나타나는 혈압, 심박동수 및 신장기능의 변화를 관찰하고, epinephrine 1 ug/kg과 10 ug/kg을 상이한 온도에 노출된 자라의 정맥내 투여하여 나타나는 효과를 비교하였다. 1) 18℃에서 25℃로 수온을 증가시킴에 따라 심박동수는 현저히 증가하여 일정하게 유지되었으나, 혈압 및 혈장 renin 활성도는 변화하지 않았다. 온도증가에 의해 뇨량, 사구체여과율 및 전해질 배설량의 현저한 증가를 보였으나 90분부터는 서서히 감소하기 시작하였다. 2) 수온 18℃에 노출된 자라에서 epinephrine은 dose-dependent한 양상으로 혈압 및 심박동수를 증가시켰으며, 다량의 epinephrine 투여시 작용시간은 현저히 연장되어 있었다. 25℃에 노출된 자라에서는 epinephrine에 의한 혈압상승 효과 및 심박동수 증가는 나타났으나, dose dependency나 작용시간의 차이는 발견할 수 없었다. 3) 동량의 epinephrine에 의한 혈압 및 심박동수의 증가효과는 18℃와 25℃에 노출된 자라에서 유의한 차이를 발견할 수 없었으나, 18℃에 노출된 자라에서 epinephrine의 작용시간 및 반감기가 현저히 연장되어 있었다. 4) Epinephrine 투여에 의해 뇨량, 사구체여과율 및 전해질 배설량의 증가를 관찰하였으며, 이는 dose-dependent 양상이었다. 그러나, 신장효과의 유의한 차이는 상이한 온도에 노출된 두 군에서 발견할 수 없었다. 이상의 결과로, 온도증가에 의한 이뇨 및 sodium 배설효과는 혈관이완에 의한 사구체여과율의 증가에 기인한 것으로 사료되며, 상이한 온도에 노출된 자라에서 epinephrine 효과의 차이를 발견할 수 없었던 것은 본 실험에서 가한 좁은 범위의 온도의 변화 내에서는 adrenoceptor의 변형이 나타나지 않을 것이라고 추론하였다. 그러나 저온에서의 epinephrine의 작용시간의 연장은 아마도 epinephrine의 파괴 효소의 활성도의 감소인 것으로 사료된다.

양성가속도가 백서의 A trial Natriuretic Peptide, Plasma Renin Activity에 미치는 영향

박종관 ( Jong Kwan Park ),서만욱 ( Man Wook Seo ),설경환 ( Kyung Hwan Seul ),이인기 ( In Kee Lee ),박병옥 ( Byung Ok Park ) 국군의무사령부 1991 대한군진의학학술지 Vol.22 No.1

Spontaneously Hypertensive Rat에 있어서 Atrial Natriuretic Peptide의 신장기능과 몇가지 호르몬 분비에 미치는 영향

김산호(Kim, San-Ho),김선희(Kim, Suhn-Hee),설경환(Seul, Kyung-Hwan),조경우(Cho, Kyung-Woo) 대한생리학회 1988 대한생리학회지 Vol.22 No.1

The present study was undertaken to clarify the involvement of atrial natriuretic peptide in the development of hypertension in spontaneously hypertensive rats. Plasma concentration of immunoreactive atrial natriuretic peptide was higher in spontaneously hypertensive rats than in normotensive Sprague-Dawley and Wistar rats. Plasma renin concentration was lower in SHR than in normotensive rats, as observed in earlier experiments. Hydration-induced increase in urine flow and urinary excretions of sodium and potassium were smaller in SHR than in normotensive control rats. Intraarterial infusion of atrial natriuretic peptide resulted in increases in urine flow, urinary excretions of sodium and potassium in both hypertensive and normotensive rats. Renal response to atrial natriuretic peptide was markedly suppressed in SHR. Plasma renin and aldosterone concentration were suppressed by atrial natriuretic peptide in both SHR and normotensive rats. The responses were not significantly different in both groups. These results suggest that the renal responsiveness to atrial natriuretic peptide may be suppressed in SHR by some mechanisms still remaining obscure.

양성중력가속도가 조종사의 심방이뇨호르몬(Atrial Natriuretic Peptide)에 미치는 영향

박종관 ( Jong Kwan Park ),서만욱 ( Man Wook Seo ),설경환 ( Kyung Hwan Seul ),박병옥 ( Byung Ok Park ),조경우 ( Kyung Woo Cho ) 국군의무사령부 1992 대한군진의학학술지 Vol.23 No.1

양성중력가속도가 조종사의 심방이뇨 호르몬(Artrial Natriuretic peptide)에 미치는 영향

박종관,서만옥,설경환,조경우,박병옥 공군본부 의무감실 1991 항공의학 Vol.39 No.1-2

자연발생 고혈압백서 신장절편의 Renin 유리에 미치는 TMB-8의 영향

박동수,김성진,김선희,조경우,설경환 대한내분비학회 1992 Endocrinology and metabolism Vol.7 No.3

In order to determine the alteration of renin release by nephron heterogeneity in spontane -ously hypertensive rats (SHR), the responsiveness of renin release to 8-(N-N-diethylamino) octyl-3, 4, 5-trimethoxy benzoate (TMB-8) from kidney slices of SHR, Wistar rats(WR) and Sprague-Dawley rats (SDR) was compared. The basal renin release from renal slices incubated with calcium free buffer was increased more than that incubated with regular buffer. The responsiveness of renin release to TMB-8 was attenuated in kidney slices incubated with calcium free buffer. Additionally, the responsiveness of renin release to TMB-8 was attenuated in SHR, especially inner cortex of kidney. With dehydration for 3 days, renin effect of TMB-8 was accenuated in inner cortex of SHR. The results indicate that the response of renin release to TMB-8 was attenuated in inner cortex of kidney from SHR. These altered responses may be related with abnormality of calcium metabolism in the kidney of SHR. (J Kor Soc Endocrinol 7:260~266, 1992)