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Ganoderma lucidum(Fr.) Karst의 배양적(培養的) 특성(特性)에 관(關)한 연구(硏究)
서건식,신관철,박종성,Seo, Geon Sik,Shin, Gwan Chull,Park, Jong Seong 충남대학교 농업과학연구소 1988 Korean Journal of Agricultural Science Vol.15 No.1
한국산(韓國産) 영지(靈芝)버섯의 계통분류(系統分類) 및 유전형질(遺傳形質)을 탐색(探索)하기 위하여 영지균(靈芝菌)의 배양적(培養的) 특성(特性)을 조사(調査)한 결과(結果)는 다음과 같다. 1. 한국산(韓國産) 자생영지균(自生靈芝菌)은 Oat meal agar 및 Rice bran extract agar에서 균사생장(菌絲生長)이 양호(良好)하고 Mycelial sector, Mycelial strand, Concentric zone의 형성(形成)은 배지(培地)의 영향(影響)을 받으나 균주(菌株)에 따라서도 차이(差異)를 보였다. 2. 영지균(靈芝菌)은 온도(溫度) $30^{\circ}C$, pH 5.5~6.0일때 균사생장(菌絲生長)이 좋았으며 백색형광등(白色螢光燈) 조사(照射)는 균사생장(菌絲生長)을 억제(抑制)하였고 광처리(光處理)의 균사생장억제기간(菌絲生長抑制時間)은 4~8시간(時間)사이였다. 3. 균사생장중(菌絲生長中)의 Mycelial strand의 형성(形成)에는 광(光)의 조사(照射) 그리고 Mycelial sector의 형성(形成)에는 배지(培地)의 영양원(營養源)과 광(光)의 조사등(照射等) 제(諸) 요인(要因)이 관여(關與)하였고 균주간(菌株間)에도 명확(明確)한 차이(差異)를 보였다. 4. 균배양중(菌培養中) 및 균사생장후(菌絲生長後) 광조사(光照射)는 자실체형성(子實體形成)을 유도하였는데 그 영향(影響)은 균주(菌株)에 따라 뚜렷한 차이(差異)를 보였고 균사생장중(菌絲生長中) 후막포자(厚膜胞子)의 형성(形成)은 인공재배시(人工栽培時) 편각형(扁角型) 자실체(子實體)를 형성(形成)하는 균주(菌株)에서 많이 발견(發見)되었고 녹각형(鹿角型) 자실체(子實體)를 형성(形成)하는 균주(菌株)에서는 많지 않았다. In order to elucidate the systematic taxonomy and genetic characters of Canoderma lucidum, cultural characteristics of the fungus were investigated. Mycelial growth of Ganoderma lucidum were favorable on oat meal agar medium, and optimum temperature and pH of the medium for mycelial growth were $30^{\circ}C$ and 5.5-6.0 respectively. Irradiation of white fluorescent lamp inhibited mycelial growth and critical time for inhibition of mycelial growth was 4-8 hours. Concentric zones and mycelial strands of Ganoderma lucidum was induced by irradiation of white fluorescent lamp and formation of mycelial sectors was influenced by nutrient source of media and irradiation of white fluorescent lamp. These characters were different among the isolates, but no relationship was observed between these characters and the fruiting body type of the fungus. Basidiospores were formed directly from the mycelium cultured on artificial media without producing fruit body.
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한국산(韓國産) 자생(自生) Ganoderma lucidum의 형태적(形態的) 특성(特性)
신관철,박용환,서건식,차동열,Shin, Gwan Chull,Park, Yong Hwan,Seo, Geon Sik,Cha, Dong Yeul 충남대학교 농업과학연구소 1986 Korean Journal of Agricultural Science Vol.13 No.1
A total number of 916 fruiting bodies of Ganoderma lucidum (Fr.) Karsten grown naturally in Korea were collected to investigate the morphological characters and some other useful characters related to the quality of the fungus and the results obtained are summarized as follows; The host tree showing the highest parasitic rate by the Genoderma lucidum(Fr.) Karsten was oak tree and the parasitic rate of the tree was 86.2%. The order of parasitic rate of the host trees by the fungus next to the oak was peach, chestnut, persimmon and acasia. The size, shape and color of fruiting bodies were varied according to the host trees. The average size of fruiting bodies collected was 40 to 100 mm by 30 to 80mm. The fruiting bodies with pileus size of 60 by 45 mm, pileus thickness of over 10mm, pileus minor axis/stipe length ratio of over 0.65 and pileus thickness/pileus minor axis ratio of over 0.22 were considered having higher commerical values. The external shape of the fruiting body was very important criterion to evaluate the quality of the Ganoderma lucidum (Fr.) Karsten. A great variation in characters such as pileus shape, color, marginal shape, zonation of pileus, pileus thickness, poroid layer of fruiting bodies and shape of stipe were observed from the fruiting bodies of G. lucidum collected in Korea. Further studies will be necessary for the genetic nature of these characters. Ganoderma lucidum (Fr.) Karsten (불로초(不老草), 영예)의 형태적(形態的) 특성(特性)을 구명(究明)하고 한국산(韓國産) 자생종(目生種)의 품질(品質)에 관계(關係)가 있는 형질(形質)을 조사(調査)하기 위하여 916점(點)의 영예를 수집(蒐集), 조사(調査)하였다. Ganoderma lucidum의 기생율(寄生率)은 참나무류가 전체의 86.2%였고 그 다음은 복사나무, 밤나무, 감나무, 아카시아나무 등(等)이었다. 기주(寄主)에 따라 자실체(字實體)의 크기, 형태, 색택에 차이가 있었다. 수집된 자실체(字實體)의 대부분(大部分)은 크기가 $40{\sim}100{\times}30{\sim}80mm$였고 자실체(字實體)의 갓의 크기는 $60{\times}45mm$ 이상(以上) 갓두께 10mm 이상(以上) 갓크기/대길이 율(率) 0.65 이상(以上) 갓두께/갓크기 율(率) 0.22 이상(以上)인 것이 상품가치가 높다. 영예자실체의 외형(外形)은 중요(重要)한 품질평가기준(品質評價基準)이다. 갓의 색택(色澤), 주연부의 모양, 갓의 윤문(輪紋), 갓의 두께 자실층형태(子實層形態), 대의 형태(形態)는 많은 변이(變異)를 보이고 있음이 관찰(觀察)되었다. 이 형질(形質)들이 유전성(遺傳性)인 것인지는 추후 구명(究明)되어야 한다.
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Buprofezin이 느타리버섯속의 원형질체 나출 및 재생에 미치는 영향
신관철,황의일,서건식 충남대학교 농업과학연구소 1990 농업과학연구 Vol.17 No.2
Effects of buprofezin, an inhibitor of chitin synthesis, on mycelial growth, protoplast formation and reversion of Pleurotus ostreatus and P. sajor-caju were investigated. The mycelial growth of Pleurotus ostreatus and P. sajor-caju was the inhibited by buprofezin treatment, and the inhibition rate was severer as the concentration of the buprofezin increased. Aerial mycelium formation was increased by buprofezin treatment, but mycelial morphology was not changed. Protoplast formation of Pleurotus ostreatus and P sajor-caju. was significantly increased when buprofezin was added to the culture medium at the concentration of 200-500 ppm and the protoplast reversion of the mushrooms was also increased by the treatment of the buprofezin.
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Ganoderma lucidum(Fr.) Karst의 培養的 特性에 關한 硏究
徐健植,申寬澈,朴鍾聲 충남대학교 농업과학연구소 1988 農業技術硏究報告 Vol.15 No.1
In order to elucidate the systematic taxonomy and genetic characters of Canoderma lucidum, cultural characteristics of the fungus were investigated. Mycelial growth of Ganoderma lucidum were favorable on oat meal agar medium, and optimum temperature and pH of the medium for mycelial growth were 30℃ and 5.5-6.0 respectively. Irradiation of white fluorescent lamp inhibited mycelial growth and critical time for inhibition of mycelial growth was 4-8 hours. Concentric zones and mycelial strands of Ganoderma lucidum was induced by irradiation of white fluorescent lamp and formation of mycelial sectors was influenced by nutrient source of media and irradiation of white fluorescent lamp. These characters were different among the isolates, but no relationship was observed between these characters and the fruiting body type of the fungus. Basidiospores were formed directly from the mycelium cultured on artificial media without producing fruit body.
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조수묵,서건식,유승헌,유익동,신관철 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.6
우리나라에서 재배되고 있는 영지버섯(적지)과 형태적으로 다른 백색변이주의 자실체를 수집하여 형태적인 특성을 조사하고 자실체로부터 순수분리한 균사체의 형태 및 최적배양조건을 조사하였다. 그 결과 영지버섯 백색변이주의 자실체 형태는 백색으로 대가 없고 포자는 타원형 이었으며 균사는 정상주에 비하여 가늘었다. 영지버섯 백색변이주의 최적 생육배지는 potato sucrose agar 배지이었으며 균사생육 최적 pH는 5.5이었다. 영양원으로 탄소원은 D-sorbitol과 carboxyl methyl cellulose를, 질소원으로는 무기태 질소인 Ca(NO_2)_2를, 비타민류는 nicotinic acid를 가장 잘 이용하였으며, 무기염류간에는 큰 차이가 없었다. A morphologically different form of Ganoderma lucidum was isolated from a cultivator’s farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white whereas those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5. The mutant preferred carboxyl methyl cellulose and calcium nitrate [Ca(NO_2)_2] as a carbon and nitrogen sources, respectively. Nicotinic acid was a favorable vitamin source for growth of the mutant. No significant differences were observed in utilization of inorganic salts.
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