한우(韓牛)에 발생(發生)하는 Cystic Ovaries 에 관(關)한 연구(硏究)

변명대,장인호,Byun, Myung Dae,Jang, In Ho 대한수의학회 1971 大韓獸醫學會誌 Vol.11 No.1

During three months from December 1967 to February 1968, 1867 Korean native cows slaughtered in pusan abattoir were investigated for studying the incidence of cystic ovaries. The results obtained in this experiment are summarized as follows: 1. The average frequency of cystic ovaries in 1867 Korean cows was 17 cases, or 0.91%. 2. In 17 cases 1, or 0.3%, occurred in cattle from 1 to 3 years of age; 4 or, 1.02% in four; 7 or, 2.02% in five; 2 or, 0.63% in six; and 3, or 0.63% occurred between 7 and 9 years of age. 3. The incidence of cysts in the respective ovaries, the left ovary was cystic in 23.5% of the cases, the right one in 41.2% and both ovaries in 35.3%. 4. According to the histological findings of cystic ovaries, follicular cysts were 88.2% and luteal cysts 11.8%.

Prostaglandin F2α 및 Dexamethasone 의 투여가 자성 Rat 의 생식기에 미치는 영향

변명대 ( Myung Dae Byun ) 한국축산학회 1983 한국축산학회지 Vol.25 No.1

The present study was conducted to determine the effects of PGF₂α and dexamethasone on corpus luteum in pregnant rats. PGF₂α and dexamethasone were injected separately and simultaneously subcutaneously twice a day for 4 days beginning on day 10 of pregnancy. The plasma progesterone levels were measured following each injections. Also the ovarian, uterine, luteal and adrenal gland weights, number of corpora lutea, as well as number and size of implants were investigated. Ovaries and adrenals were examined by light microscopy. The results obtained were summarized as follows: 1. Treatment with PGF₂α or dexamethasone separately or in combination reduced significantly the plasma progesterone levels to 81.4 ± 0.7, 27.3 ± 0.6 and 28.6 ± 0.6 ng/㎖ respectively, compared to 135.7 ± 0.7 ng/㎖ in the control rats (p$lt;0.01). 2. The weights of ovaries and corpora lutea and the number of corpus luteum were slightly decreased as compared to the control group but the difference was not statistically significant in the groups. 3. The uterine weights and the number and size of implants were significantly decreased as compared to the control group (p$lt;0.01). They were decreased markedly in the two groups of rats receiving PGF₂α treatment alone and PGF₂α/dexamethasone combined as compared to those of rats treated with dexamethasone only. 4. The adrenal gland weights were not affected by treatment with PFG₂α or dexamethasone alone or in combination. 5. In the histological findings of adrenals of the dexamethasone treated rats, there was an atrophy of the adrenal cortex, hyperemia, hemorrhage, vacuolization and necrosis of the cortical cells. 6. In the histological findings of ovaries for the PGF₂αtreated rats, pyknotic nuclei and cytoplasrnic vacuolization of luteal cells were observed. In the dexamethasone treated rats, congestion and hemorrhage, varuolization and necrosis of luteal cells and necrosis of granulosa cells were observed. Also the corpus luteum contained necrotic foci.

한국재래산양(韓國在來山羊)에 있어서 Prostaglandin $F_{2{\alpha}}$의 투여(投與)가 난소(卵巢) 및 뇌하수체(腦下垂體) Hormone의 함량(含量)에 미치는 영향(影響)

권춘수,변명대,장인호,Kwon, Chun Su,Byun, Myung Dae,Jang, In Ho 경북대학교 농업과학기술연구소 1988 慶北大農學誌 Vol.6 No.-

재래산양(在來山羊)에 있어서 prostaglandin $F_{2{\alpha}}$의 투여(投與)가 황체퇴행(黃體退行)시에 야기되는 호르몬 함량의 변화(變化)를 구명(究明)하기 위하여 발정주기의 10일에 $PGF_{2{\alpha}}$(Lutalyse) 5mg을 3시간 간격으로 2회 주사(注射)한 다음 경시적(經時的)으로 혈중(血中) 난소(卵巢) 및 뇌하수체(腦下垂體) hormone의 함량을 조사하였다. 본 연구에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 재래산양(在來山羊)에 있어서 발정주기(發情週期)의 10일에 $PGF_{2{\alpha}}$를 주사한 결과 혈중(血中) progesterone의 평균농도는 주사직전에 $4.15{\pm}1.8ng/ml$였으며 주사후(注射後) 3시간에 $2.52{\pm}1.2ng/ml$로 약 60% 감소(減少)하였으며 12시간에는 $0.81{\pm}0.3ng/ml$로 감소(減少)하여 72시간까지 1.02ng/ml 이하로 감소(減少)하였다. 혈중(血中) estradiol-$17{\beta}$ 함량은 $PGF_{2{\alpha}}$ 투여후(投與後) 6시간부터 유의(有意)하게(p < 0.05) 증가(增加)하여 72시간까지 유지되었다. 혈중(血中) LH함량은 주사직전에 $3.0{\pm}0.3{\mu}IU/ml$였으며 $PGF_{2{\alpha}}$ 투여후 3~12시간에는 증가(增加)하였으나 그후 24~72시간 사이에는 $4.1{\mu}IU/ml$에서 $2.5{\mu}IU/ml$로 감소(減少)하는 경향(傾向)을 보였다. 혈중(血中) FSH 함량은 투여전 $3.5{\pm}0.5{\mu}IU/ml$였으며 $PGF_{2{\alpha}}$ 투여후(投與後) 3시간부터 감소하기 시작하여 72시간까지 유의차없이 약간 감소(減少)를 나타내었다. 혈중(血中) prolactin의 함량은 유의성(有意性)은 없었으나 $PGF_{2{\alpha}}$ 투여후 약간 증가(增加)하는 경향(傾向)을 보였다. 이상의 결과(結果)에서 LH함량의 최초 증가는 혈장중(血漿中) progesterone의 감소로 기인된 것이며 성선척극(性腺刺戟) hormone의 분비형태(分泌形態)는 황체(黃體)의 퇴행시에 progesterone 또는 progesterone과 estradiol-$17{\beta}$의 함량비의 차이로 인하여 상위되었다고 본다. This experiment was conducted to examine the $PGF{2{\alpha}}$-induced changes in concentrations of ovarian and pituitary hormones of Korean native goats. Each goats received two injections of $PGF{2{\alpha}}$ (5mg each ; 3 hours apart) on day 10 of the estrous cycle. Jugular venous blood samples were collected at 0, 3, 6, 12, 24, 48 and 72 hours postinjection for quantification of LH, FSH, prolactin, progesterone and estradiol-$17{\beta}$. The results were summarized as follows ; The blood serum concentration of progesterone was decreased from pretreatment level of $4.15{\pm}1.8ng/ml$ to $2.52{\pm}1.2ng/ml$ (about 60%) within 3 hours and to $0.81{\pm}0.3ng/ml$ at 12 hours of the $PGF{2{\alpha}}$ injection. After 12 hours, the concentrations of progesterone were less than 1.02ng/ml by 72 hours postinjection. The concentrations of estradiol-$17{\beta}$ following treatment increased (p < 0.05) over the 72 hours. Initial concentration of LH was $3.0{\pm}0.3{\mu}IU/ml$. After treatment with $PGF{2{\alpha}}$, concentrations of LH increased within 12 hours but declined 12 and 72 hours from $4.1{\mu}IU/ml$ to $2.5{\mu}IU/ml$. Prior to administration of $PGF{2{\alpha}}$, mean concentration of FSH was $3.5{\pm}0.5{\mu}IU/ml$. Concentrations of FSH declined over time in goats treated with $PGF{2{\alpha}}$ on day 10 postestrus. The mean prolactin concentrations in the blood serum after $PGF{2{\alpha}}$ treatment were not significantly different from those of the pretreatment. It is concluded that the initial increase in LH is dependent on a decrease in serum progesterone and differences in patterns of secretion of gonadotropins might be caused by differences in progesterone or progesterone-estradiol ratio when luteal regression is induced on day 10 of the estrous cycle.

Dexamethasone , Nor - adrenalin 및 ACTH 의 투여가 자성 Rat의 생식기에 미치는 영향

함태수,변명대 ( Tae Su Ham,Myung Dae Byun ) 한국축산학회 1982 한국축산학회지 Vol.24 No.4

The present study was initiated to examine the effects of dexamethasone, nor-adrenalin and ACTH on the reproductive organs of immature female rats. 21day old female rats caged in groups of dexamethasone noradrenalin and ACTH, received oradexon, nor-adrenalin and ACTH subcutaneously daily for 10 days or 21 days, respectively. Rats were sacrificed 24 hr after the last injection. The results obtained in this experiment were as follows: 1. Body weight was significantly (p $lt;0.01) decreased in the dexamethasone-treated rats when compared with the control group. But noradrenalin and ACTH-treated groups were not affected significantly by chronic nor-adrenalin and ACTH treatment for 10 days or 21 days. 2. Mean ovarian weight of immature female rats injected with dexamethasone, nor-adrenalin and ACTH for 10 days were significantly (p $lt; 0.01) reduced as compared to that of control group. The ovarian weight of noradrenalin-treated group increased significantly (p$lt;0.05) after 21 days of treatment with nor-adrenalin. Dexamethasone ACTH-treated group had no significant difference, but they tend to decrease. 3. Uterine weight of female rats decreased significantly (p $lt; 0.01) after injection of dexamethasone for 10 days but noradrenalin treated group receiving nor-adrenalin increased significantly (p $lt; 0.01). Groups injected with dexamethasone, noradrenalin and ACTH for 21 days had no significance but dexamethasone-thasone-treated group tends to decrease in weight of uteri. 4. Adrenal weight of female rats receiving dexamethasone and noradrenalin for 10 days were significantly decreased, while ACTH-treated group were significantly greater (p$lt;0.01) than in rats receiving dexamethasone and nor adrenalin. Dexamethasone group were significantly decreased as compared to that of control group after 21 days of treatment of dexamethasone but ACTH-treated group were significantly increased. Noradrenalin treated group had no significance but it tends to decrease. 5. Compared to those of controls, Liver were 69.8 % lighter in 31-day old females, while spleens were 185.3% heavier. The weights of the liver and spleen were reduced by 64.5 % and 27.2 % respectively in 42-day old female. Kidney and heart weights were not affected significantly by chronic dexamethasone treatment. 6. In histological findings of rat ovaries treated with dexamethasone, granulosa cells had necrosis and the majority of follicles showed varying degrees of atresia. 7. Histological appearances of the ovaries showed numerous follicles after 10 days of treatment of nor-adrealin. Luteal cells showed proliferation of fibroblast and vacuolization after 21 days of treatment. Histological studies indicated that corpora lutea showed retrogressive changes. 8. In the overies of ACTH-treated rats for 10 days, granulosa cells of follicles were ruptured and follicular atresia were increased. Luteolysis was observed in rat ovaries treated with ACTH for 21 days but was not remarked as compared to that of noradrenalin treated group.

경북지방(慶北地方)의 육성우(育成牛) 및 비육우(肥育牛)에 있어서 기생충(寄生蟲)의 감염(感染)과 질병실태조사(疾病實態調査)

이차수,이재현,변명대,박청규,이희석,문무홍,Lee, Cha Soo,Lee, Jae Hyun,Byun, Myung Dae,Park, Cheong Kyu,Lee, Hi Suk,Moon, Moo Hong 대한수의학회 1980 大韓獸醫學會誌 Vol.20 No.2

A survey was made from March 1978 through Feb. 1979 to know infection rate of parasites and incidence of diseases in 1968 cattle of 240 rearing and fattening cattle herds (1746 Korean native cattle, 186 Holstein, 34 Charolais and 2 Hereford) raising in 28 cities and counties of Gyeongbug district. The results obtained are summarized as follows: 1. The rate of nematoda infection was about 49.0% in the examined cattle. These nematodes were identified as Mecistocirrus sp., Oesophagostomum sp., Bunostomum sp., Trichostrongylus spp., Strongyloides sp. and other 3 species. Infection rate of nematoda in fattening and breeding cattle was higher than that in rearing calves, 44.3% of the positive cattle were infected with 2 to 5 species, and incidence of mixed infection was high in fattening cattle. 2. The rate of coccidal infection was 10.9%. Eimeria $z{\ddot{u}}rni$, Eimeria bovis and Eimeria bukidnonensis were mainly found in the examined cattle. The infection rates of cocidia in Holstein, breeding Korean cows, rearing Korean calves and fattening Korean cattle were 27.3%, 15.8%, 11.2% and 9.1%, respectively. 3. The rate of trematoda was 14.6% with Fasciola spp. (11.2%), Eurytrema spp. (2.0%), and Paramphistomum spp. infections (1.4%). Breeding Korean cows, fattening Korean cattle, Holstein and rearing Korean calves indicated 47.4%, 26.4%, 18.2% and 6.3% incidence of trematoda infections, respectively. of trematoda positive cattle, 76.6% were infected with Fasciola spp., 4. The rate of Theileria and Babesia infection was 62.7% in the examined cattle. Of these positive cattle 5.4% were infected with both parasites, 57.3% with Theileria, and 1.3% of rearing Korean calves and 22.6% of Holstein were found to have parasitized erythrocytes over 1.0%. 5. The incidence of diseases in 1968 cattle of 240 herds was 564 cattle (28.7%) of 154 herds (64.2%). of the diseases observed of skin occurred in 354 cattle (18.0%) of 61 herds (25.4%), diseases of respiratory system in 121 cattle (6.1%) of 34 herds (14.2%), diseases of digestive system in 38 cattle (1.9%) of 22 herds (9.2%), diseases of eye in 21 cattle (1.1%) of 13 herds (5.4%), diseases of urogenital system in 8 cattle (0.4%) of 8 herds (3.3%) and the other diseases in 24 cattle (1.2%) of 22 herds (9.2%). 6. Diseases of skin in 1968 cattle of 240 herds werds were observed in the highest incidence. Ringworm was observed in 13.3% of the examined herds, rearing Korean calves and Holstein showed high incidence. Scabies caused by Chorioptes bovis and alopecia localis along with infestation of lice (Damalinia bovis, Linognathus vituli) were observed in fattening Korean cattlein winter. 7. Diseases of respiratory system occurred frequently in rearing Korean cattle and Holstein. In the diseases of digestive system acute indigestion occurred in fattening Korean cittle, enteritis in rearing Korean calves and Holstein, and coccidiosis in rearing Korean calves maninly. 8. Infectious keratoconjunctivitis occurred in a fattening Korean cattle herd, sterility (2.0%) in breeding Korean cows, and theileriosis in Holstein calves were also occurred. In addition, poisoning and heat stroke were observed in several cattle.

지하수와 지표수가 병아리의 발육에 미치는 영향

조헌조 ( Hean Jo Cho ),변명대 ( Myung Dae Byun ) 한국축산학회 1972 한국축산학회지 Vol.14 No.1

This experiment was an attempt to know whether surface water and ground water affect the growth rate of chicken. One hundred and twenty chicken were divided into two groups supplied with surface water and ground water, respectively, for 8 weeks. The results were summarized as follows: (1) The chicken supplied with ground water showed significantly higher growth rate than did another group. (2) The amounts of average daily consumption of water and feed were not significantly different from each other. (3) Neither the surface water nor the ground water affected the mortality rate.

韓國在來山羊에 있어서 Prostaglandin F₂α의 投與가 卵巢 및 腦下垂體 Hormone의 含量에 미치는 影響

權春水,張仁浩,邊明大 慶北大學校 農業科學技術硏究所 1988 慶北大農學誌 Vol.6 No.-

在來山羊에 있어서 prostaglandin ??의 投與가 黃體退行시에 야기되는 호르몬 함량의 變化를 究明하기 위하여 발정주기의 10일에 ??(Lutalyse) 5mg을 3시간 간격으로 2회 注射한 다음 經時的으로 血中 卵巢 및 腦下垂體 hormone의 함량을 조사하였다. 본 연구에서 얻어진 結果를 要約하면 다음과 같다. 在來山羊에 있어서 發情週期의 10일에 ??를 주사한 결과 血中 progesterone의 평균농도는 주사직전에 4.15±1.8ng/ml였으며 注射後 3시간에 2.52±1.2ng/ml로 약 60% 減少하였으며 12시간에는 0.81±0.3ng/ml로 減少하여 72시간까지 1.02ng/ml 이하로 減少하였다. 血中 estradiol-17β 함량은 ?? 投與後 6시간부터 有意하게(p <0.05) 增加하여 72시간까지 유지되었다. 血中 LH함량은 주사직전에 3.0±0.3μIU/ml였으며 ?? 투여후 3∼12시간에는 增加하였으나 그후 24∼72시간 사이에는 4.1μIU/ml에서 2.5μIU/ml로 減少하는 傾向을 보였다. 血中 FSH 함량은 투여전 3.5±0.5μIU/ml였으며 ?? 投與後 3시간부터 감소하기 시작하여 72시간까지 유의차없이 약간 減少를 나타내었다. 血中 prolactin의 함량은 有意性은 없었으나 ?? 투여후 약간 增加하는 傾向을 보였다. 이상의 結果에서 LH함량의 최초 증가는 血漿中 progesterone의 감소로 기인된 것이며 性腺刺戟 hormone의 分泌形態는 黃體의 퇴행시에 progesterone 또는 progesterone과 estradiol0-17β의 함량비의 차이로 인하여 상위되었다고 본다. This experiment was conducted to examine the ??-induced changes in concentrations of ovarian and pituitary hormones of Korean native goats. Each goats received two injections of ??(5mg each; 3hours apart) on day 10 of the estrous cycle. Jugular venous blood samples were collected at 0, 3, 6, 12, 24, 48 and 72hours postinjection for quantification of LH, FSH, prolactin, progesterone and estradiol-17β. The results were summarized as follows; The bolld serum concentration of progesterone was decreased from pretreatment level of 4.15±1.8ng/ml to 2.52±1.2ng/ml (about 60%) within 3 hours and to 0.81±0.3ng/ml at 12 hours of the ?? injection. After 12 hours, the concentrations of progesterone were less than 1.02ng/ml by 72 hours postinjection. The concentrations of estradiol-17β following treatment increased (p <0.05) over the 72 hours. Initial concentration of LH was 3.0±0.3μIU/ml. After treatment with ??, concentrations of LH increased within 12 hours but declined 12 and 72 hours from 4.1μIU/ml to 2.5μIU/ml. Prior to administration of ??, mean concentration of FSH was 3.5±0.5μIU/ml. Concentrations of FSH declined over time in goats treated with ?? on day 10 postestrus. The mean prolactin concentrations in the blood serum after ?? treatment were not significantly different from those of the pretreatment. It is concluded that the initial increase in LH is dependent on a decrease in serum progesterone and differences in patterns of secretion of gonadotropins might be caused by differences in progesterone or progesterone-estradiol ratio when luteal regression is induced on day 10 of the estrous cycle.

Prostaglandin F_2α의 投與가 姙娠 Rat의 黃體에 미치는 影響

邊明大,李熙碩 慶北大學校 1982 論文集 Vol.34 No.-

These experiments were carried out to investigate the weighs of the ovaries and corpora lutea, number of corpora lutea and luteal cells, weight of uterus and number of embryo, as well as morphological changes in corpus luteum from 30 minutes to 96 hours following a single subcutaneous injection of a luteolytic dose (1500 μg/rat) of prostaglandin F_2α(PG-F_2α) to pregnant rats (day 10 post coitum). Histological changes of corpora lutea in pregnant rats injected with PGF_2α were examined by light microscopy at the respective times. The results obtained in this study were summarized as follows; 1. The weights of the ovaries and corpora lutea were decreased slightly at 18 to 24 hours of PGF_2α administration but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12 to 48 hours and 18 to 24 hours of PGF_2α injection but there were no significant differences between control and treatment. 3. The weight of uterus and the number of embryo decreased slightly at 96 hours and at 18 to 96 hours of PGF_2α administration but no significant differences were obtained. 4. In the light microscopic observation of corpora lutea, moderately extented blood vessels of central portion, appearance of lipid droplets, cytoplasmic vacuolation and pyknosis in luteal cells were observed at 1.5 hours after PGF_2α injection. These changes were more evident at 24 hours and those findings recovered to nomal at 96 hours of treatment.

H.C.G.를 投與한 産羊에 잇어서 Anti-H.C.G.의 産成에 관한 硏究

邊明大 건국대학교 1978 論文集 Vol.8 No.1

Three goat were injected with H. C. G. to determine the effect of dosage and frequency of injection upon the production of antigonadotropins, The three female goat were treated once weekly with various doses of H. C. G. injected subcutaneously. The results obtained were summarized as follows : 1. Anti-HCG activity appeared by the 2nd week with injection of 500 1u (G), by the 3rd week with 1,000 1u (G), and by th 5 week with the highest dose (2,000 1u, G). 2. Anti-HCG activity in G treated with 2,000 1u of HCG did not reach a peak until about 7th to 9th week but maximum potency was reached in 3 to 7 weeks in goat (G , G) receiving continuous once-weekly injections. 3. Antigonadotrophins were not detected in the sera of untreated goat (control and goat before treatment). The lowest of HCG injected (500 1u per week) brought about a more rapid production and earlier peak activity than did the higher dose level (2,000 1u group). 4. Anti-HCG serum completely inhibited to the activity of as much as 5 1u of HCG. It is concluded that antihormane formation is a serous factor in the clinical use of HCG in animals if minimal physiological doses are not employed.

大邱近郊에 飼育되는 乳牛의 第 1 胃 內容物의 調査 分析 : pH, 纖毛蟲 및 揮發性脂肪酸 ph, Ciliate Protozoa and Volatile Fatty acids

李熙碩,邊明大,咸泰守 경북대학교 1981 새마을 硏究論叢 Vol.1 No.-

The Content of rumen were compared between two groups (A and B) of dairy cow fed different rations. Group A were fed on ration consisted exclusively of concentrates, and Group B were fed on ration consisted mainly of roughage. 1. The pH of content in the rumen showed that group A was 6.9 and group B was 6.5 on the average. 2. Total number of ciliates of both A and B groups on the average per ml showed normal range, displaying somewhat increased in group A than group B. Ten genera or sub-genera and twenty-five species of ciliates were detected from the content of the rumen both A and B group of dairy cow. Among them Betehella was detected for the first in Korea. Entodinium simplex keeps the highest appearance rate of all species of ciliates, showing above 60 per cent of them all, and the others showing below 7 per cent on average. 3. There was no significant difference in volatile fatty acid(VFA) concentration of the contents of the rumen between two groups. When the VFA composition of the contents of the rumen was compared, the acetic acid content was significantly higher (P<0.05) whereas the propionic acid content was significantly lower (P<0.05) in group A than in those of group B.