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사람 장내세균군집 유래 Bacteorides stericoris HJ-15의 Chondroitinase ABC의 클로닝
방서현 ( Seo Hyeon Bang ),심주원 ( Juwon Shim ),현양진 ( Yang Jin Hyun ),김동현 ( Dong Hyun Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.2
사람의 분변으로부터 분리한 Bacteroides stericoris HJ-15로부터 chondroitinase ABC 유전자를 클로닝하였다. 클로닝한 chondroitinase ABC 유전자는 3,090 bp, 1,029 아미노산으로 구성되어 있었다. B. stercoris chondroitinase ABC 유전자는 이미 보고된 chondroitinase ABC 유전자들과 호몰로지가 없었으나, 아미노산서열에서는 82% 호몰로지를 보였다. T7 promoter를 가진 pET-26b+ expression vector에 클로닝한 chondroitinase ABC 유전자를 Escherichia coli BL21 (DE3)에서 발현하여 정제한 재조합 chondroitinase ABC는 chondroitin sulfate A, B 및 C를 모두 분해하였다. The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029-amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron. The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.
김진경,방서현,이상일 한국마린엔지니어링학회 2022 한국마린엔지니어링학회지 Vol.46 No.6
As the mandatory code for ships using gases or other low-flashpoint fuels came into force on January 1, 2017, the new training requirements for seafarers working on such ships have become a challenge for Maritime Universities. This study aimed to promptly support the seafarers’ training system in compliance with the Standards of Training, Certification and Watchkeeping (STCW) amendment. The methodology of this study is outlined in the following three major steps: first, the analysis of the relationship between international convention and domestic law concerning the International Code of Safety for Ships Using Gases or Other Low-flashpoint Fuels (IGF) Code; Second, the identification of the minimum requirement of education for seafarers on ships subject to the IGF Code; and Third, practical recommendations for updating the existing training system. In particular, the study suggests that critically pressing is the need for (ⅰ)simulator training for eco-friendly ships; (ⅱ) the construction of training ships applying the IGF code for Maritime Universities, and (ⅲ) collaboration with shipbuilding yards in providing cadet officers and engineers with training opportunities in bunkering. The overall objective is to contribute to the practical training system needs of Maritime Universities in response to the rapidly changing regulatory environment.