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Enhancement of Capturing Efficacy for Circulating Tumor Cells by Centrifugation
방도연,이택수,박주형,이규도,함승주,박진성 한국바이오칩학회 2018 BioChip Journal Vol.12 No.1
Circulating tumor cells (CTCs), which are thought to be the main candidate for metastasis, are gaining importance owing to their potential impact on human health and public welfare. For capturing CTCs, antigen-antibody interaction has been used in which specific antigens expressed on cell surface of CTCs can be bound to antibodies immobilized on substrates. Conventional detection methods for CTCs have often suffered not only from relatively low antigen-antibody coupling efficiency but also from cumbersome fabrication processes of micro/nano-structures for CTCs capture. Herein, we report a facile, robust antibody-based CTCs detection technique using centrifugal force, which can enhance the capturing efficacy of CTCs. We validate chemical functionalization process of antibodies on a silica substrate by using atomic force microscopy. Furthermore, it turned out that the centrifugal force from a benchtop centrifuge is enough to produce a ~2.3-fold increase in capture yield of CTCs through enhancement of binding avidity between CTCs and the antibodies. Our result points out the great potential of our method for practical application in CTCs diagnostics and opens a new avenue for biological and chemical sensing.
악력과 정신 건강의 연관성: 국민건강영양조사(2015년, 2017년)
현보라,윤영숙,양윤준,이언숙,이준형,허 연,정창현,방도담,강보람 대한가정의학회 2021 Korean Journal of Family Practice Vol.11 No.5
Background: Handgrip strength (HGS) is a commonly measured indicator of physical activity. As decreases in HGS are associated with mortality andcardiovascular morbidity, it is an important biomarker of disease and a health status. Many studies have investigated the association between HGSand chronic diseases in the elderly, but the relationship of HGS with mental health has been less studied. Therefore, this study aimed to identify theassociation between HGS and psychological distress (depressive symptoms and suicidal ideation). Methods: Study subjects were 9,589 adults aged ≥19 years who participated in the Korean National Health and Nutrition Examination Survey in 2015and 2017. HGS was measured three times in alternating hands, and the maximum value of HGS of the commonly used hand was used as the finalgrip value. A logistic regression model was used to analyze the associations of HGS with depressive symptoms and suicidal ideation after adjustingfor covariates. Results: The prevalence of suicidal ideation and depressive symptoms was 4.1% and 9.4% in male and 5.2% and 14.9% in female, respectively. Femaleshowed significantly higher prevalence than male (all P<0.05). In multivariable logistic regression analysis, the odds ratios (95% confidence intervals)for suicidal ideation and depressive symptoms in the highest tertile of HGS relative to the lowest tertile were 0.54 (0.30–0.95) and 0.68 (0.47–0.99) inmale and 0.63 (0.43–0.93) and 0.72 (0.57–0.92) in female, respectively. Conclusion: HGS was inversely associated with depressive symptoms and suicidal ideation, and can thus serve as a useful predictor of psychologicaldistress 연구배경: 악력은 신체 활동의 지표이며 악력의 감소는 사망률이나심혈관 질환의 이환율과 관련되어 있다. 따라서 악력은 건강상태를나타내는 중요한 생체지표로 사용될 수 있다. 악력에 관한 많은 연구에서 노인의 악력과 만성질환과의 연관성을 조사하였으나 악력과정신건강과의 연관성에 대한 연구는 많이 이루어지지 않았다. 이에악력과 정신건강문제(우울감, 자살사고)와의 연관성을 확인하기 위해 이 연구를 시행하였다. 방법: 연구대상은 2015년, 2017년 국민건강영양조사에 참여한 19세이상 성인 9,589명이다. 악력은 양손을 번갈아서 3회씩 측정하였으며 주로 사용하는 손의 최댓값을 최종 악력 값으로 사용하였다. 악력과 우울감, 자살사고와의 연관성을 파악하기 위해 공변량을 보정한 후 로지스틱 회귀분석(logistic regression)을 시행하였다. 결과: 자살 사고와 우울증상의 유병률은 각각 남자에서 4.1%, 9.4%였고, 여자는 5.2%, 14.9%로 여성에서 유의하게 높았다(all P<0.05). 다변량 로지스틱 회귀 분석에서 악력이 가장 낮은 삼분위수군에 비해 가장 높은 삼분위수군의 자살사고와 우울증상의 위험은 남성에서 각각 0.54 (0.30–0.95) (odds ratio [95% confidence interval])와 0.68(0.47–0.99)로 나타났고 여성에서 각각 0.63 (0.43–0.93), 0.72 (0.57–0.92)로 나타났다. 결론: 한국 성인 남녀에서 악력은 자살사고와 우울감과 음의 연관성이 있었으며, 본 연구는 비용이 저렴하고 측정이 간단한 악력을자살사고나 우울감을 예측할 수 있는 도구로 사용할 수 있는 가능성을 보여주었다.