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Current Strategies of Genomic Modification in Livestock and Applications in Poultry
박태섭 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.2
Since the development of the first genetically-modified mouse, transgenic animals have been utilized for a wide range of industrial applications as well as basic research. To date, these transgenic animals have been used in functional genomics studies, disease models, and therapeutic protein production. Recent advances in genome modification techniques such zinc finger nuclease (ZFN), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats (CRIPSR)-Cas9, have led to rapid advancement in the generation of genome-tailored livestock, as well as experimental animals; however, the development of genome-edited poultry has shown considerably slower progress compared to that seen in mammals. Here, we will focus primarily on the technical strategies for production of transgenic and gene-edited chickens, and their potential for future applications.
박태섭,김시원,이정효 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6
Objective: Transgenic technology is widely used for industrial applications and basic research. Systems that allow for genetic modification play a crucial role in biotechnology for a number of purposes, including the functional analysis of specific genes and the production of exogenous proteins. In this study, we examined and verified the cumate-inducible transgene expression system in chicken DF1 and quail QM7 cells, as well as loxP element-mediated transgene recombination using Cre recombinase in DF1 cells. Methods: After stable transfer of the transgene with piggyBac transposon and transposase, transgene expression was induced by an appropriate concentration of cumate. Additionally, we showed that the transgene can be replaced with additional transgenes by co-transfection with the Cre recombinase expression vector. Results: In the cumate-GFP DF1 and QM7 cells, green fluorescent protein (GFP) expression was repressed in the off state in the absence of cumate, and the GFP transgene expression was successfully induced in the presence of cumate. In the cumate-MyoD DF1 cells, MyoD transgene expression was induced by cumate, and the genes controlled by MyoD were upregulated according to the number of days in culture. Additionally, for the translocation experiments, a stable enhanced green fluorescent protein (eGFP)-expressing DF1 cell line transfected with the loxP66-eGFPloxP71 vector was established, and DsRed-positive and eGFP-negative cells were observed after 14 days of co-transfection with the DsRed transgene and Cre recombinase indicating that the eGFP transgene was excised, and the DsRed transgene was replaced by Cre recombination. Conclusion: Transgene induction or replacement cassette systems in avian cells can be applied in functional genomics studies of specific genes and adapted further for efficient generation of transgenic poultry to modulate target gene expression.
박태섭,김영란 명지대학교 예체능연구소 2003 藝體能論集 Vol.14 No.-
The purpose of this study was to determine health Index for elementary school students based on their health and physical-condition records in an effort to help them check heir health status, provide authentic health information and suggest how to take advantage of health records The subjects were 400 boy and girl students in their fifth and sixth grade of elementary school in Incheon. For data handing. t-test, ANOVA test, Pearson correlation coefficient, multiple regression analysis, principal component analysis and Cronbach's a were employed, And SPSS Win 100 program was utihzed. The findings of this study were as below. First, the boy students had a better physical condition in every area than the girls, and the slxth grades grew better than the fifth grades at the p,.001 level of significance. Second, concerning the development of physical fitness, the sixth grade boys excelled the fifth grade boys at every area except sit and reach, and the gsp between them was more significant in 50m run dash, standing broad jump and set-ups. In the case of the girls, the sixth grades outperformed the fifth grades in every area, but the disparity between the two was insignificant. By gender, the girls significantly outdid the boys in sit and reach, but the boys had a significantly better physical fitness than the girls in the other areas. Third, the Width and volume seemed to be more related to physical development than height and sitting height. Although the contribution of physical condition to physical fitness depends on school year and gender, height and sitting height made a less prediction of physical development than the width and volume did. Fourth, when their fitness level was assessed by physical-condition index, the width and volume developed better than height and sitting height, and the students made the best progress in endurance run and walk. Fifth, concerning the impact of health habits on fitness level, they were most healthy at the time of workout. Their gender made a significant difference to fitness level during exercise, as the boys were more healthy than the girls at the p<.001 level of significance.