天皇巡幸의 展開와 民衆 : 천황순행의 전개와 민중

박진우 부산대학교 사학회 1999 釜大史學 Vol.23 No.-

정상 인체 멜라닌세포에서 endothelin-1 receptor antagonist가 melanin생성에 미치는 영향에 관한 연구

박진우,이종석,황규왕,김정하 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

In the normal skin, melanocytes maintain their constant population and their melanogenic activity in steady state, but respond to several stimulus such as UV radiation and chemical irritation, to accentuate proliferation and differentiation, leading to hyperpigmentation of the skin. Human Keratinocytes produce and secrete endothelin-1, which can be strong mitogens for human melanocytes. The paracrine linkage of endothelin-1 between keratinocytes and melanocytes suggested that endothelin-1 is intrinsic mediators for human melanocytes in UVB-induced pigmentation. The purpose of this study is to investigate the new whitening agent, which improve the hyperpigmentation by inhibiting the ET-1. So, we add ET-1 and five kinds of ETRA into the cultured normal human melanocyte and then three days later, each ETRA on ET-1 was measured melanocyte number and inhibitory effect of melanin synthesis, respectively. In this study, ETRA has a inhibitory effect on ET-1 induced melanocyte proliferation and melanogenesis. ETRA I has a strong inhibitory effect on melanocyte proliferation and ETRA Ⅱ has a strong inhibitory effect melanogenesis. western blotting results shows negative. It may be due to the processing error of protein denaturing or low antigen -antibody affinity. But, we also confirm that ETRA Ⅱ has a inhibitory effect of tyrosinae synthesis by RT-PCR

PDGF-BB 적용시간이 decalcified dentin에서의 치은섬유아세포의 증식에 대한 효과

박진우,이재목,서조영,Park, Jin-Woo,Lee, Jae-Mok,Sun, Jo-Young 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of deminera1ized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using deminera1ized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/I0% FBS at the $37^{\circ}C$, 5% CO2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCI(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/rnl PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of $1{\times}10^5$cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows : The application of PDGF-BB for 1, 2 min slightly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominently increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingival fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.

韓國人頭蓋骨의 X線學的 測定値

朴鎭雨,金斗祚 慶北大學校 1964 論文集 Vol.8 No.-

1. Statistical measurements are done on normal Korean adult skull, male 100 persons, female 40 persons, total 150 persons, for the length, breadth, cephalic index, heihgt, A-P diameter and depth of the sella turcica, basal angle, facial angle and calcification of teh pineal gland. 2. Most of the Korean skulls (85%) are brachycephalic skull. 3. Calcification of the pineal gland is seen in 18%.

새로운 출발을 위하여

박진우 샘터사 2003 월간 샘터 Vol.34 No.4

확률화 회답모형의 정도(精度)에 관한 연구

박진우 수원대학교 기초과학연구소 1993 基礎科學論文集 Vol.2 No.-

The performances of Randomized Response Models are investigated. RRM provides a method to get more reliable informations about highly personal matters. However, when the proportion of a sensitive attribute is very small, the precision of estimators from RRM may be poor. In that case use of RRM is restricted. In this paper, the performances of RRM are investigated when population proportion of the sensitive attribute changes.

탈회된 상아질면에 혈소판유래성장인자-BB적용시간이 치은섬유아세포의 증식에 미치는 영향

박진우 慶北大學校 齒科大學 1996 慶北齒大論文集 Vol.13 No.-

섬유아세포의 이주와 증식에 중요한 역할을 하는 것으로 알려진 PDGF-BB를 이용하여 테트라사이클린으로 탈회시킨 치근면에 PDGF-BB의 다양한 적용시간에 따른 증식세포수를 측정해 봄으로써, PDGF적용시간이 섬유아세포의 증식에 미치는 효과를 알아보기 위하여 본 실험을 실시하였다. 교정치료를 목적으로 내원한 환자의 발거한 소구치를 이용하여 상아질절편을 제작하여 시편으로 사용하였다. 100mg/ml 농도의 테트라사이클린으로 5분간 탈회시킨 시편을 50ng/ml 농도의 PDGF-BB로 30초, 1분, 2분, 4분, 8분 동안 처리하고 성장인자를 적용하지 않은 시편을 대조군으로 하여 각 시편을 24well 조직 배양접시에 넣고 1 × 10 exp (5)개의 치은섬유아세포를 가진 배양액 1ml 씩을 넣어 6시간 배양한 후 새로운 배양접시로 옮기고 24, 48, 72시간동안 배양하여 각 시간대별로 0.05% trypsin/0.02% EDTA로 처리하고 세포를 분리하여 광학위상차현미경하에서 세포수를 측정하고 시편의 단위면적당 세포수를 계산하여 다음과 같은 결과를 얻었다. 세포증식률의 측정실험에서 24, 48, 72시간 공히 대조군과 비교시 PDGF-BB 적용시간의 증가에 따라 증식세포수가 증가하였으며 4분 및 8분 적용군에서 세포증식률의 현저한 증가양상을 보였다. PDGF-BB적용군과 테트라사이클린만 처리한 대조군과 비교시 24, 48시간에서는 PDGF-BB 1분 적용군부터 세포수가 증가를 관찰할 수 있었으나 적용시간 2분까지는 그 정도가 미약하였으며 4,8분 적용군에서 24,48,72시간 모두에서 대조군과 비교시 세포수 증가가 현저한 것을 관찰할 수 있었다. PDGF-BB 적용군사이에서는 4분 적용군이 세포증식률이 가장 높은 것으로 나타났고, 8분 적용군에서는 4분 적용군에 비해 24, 48, 72시간 모두에서 세포증식률이 감소하는 경향을 보였다. 이상의 연구를 미루어 볼 때 치은섬유아세포의 증식률에서 PDGF-BB 4분 적용군이 24, 48, 72시간 모두에서 타군과 비교시 현저한 증가를 보였으므로 탈회된 상아질을 PDGF-BB의 주적용부로 하였을때 50ng/ml농도의 PDGF-BB사용시 4분정도의 적용시간이 섬유아세포의 증식을 이루는데 있어서 가장 적절한 시간이 될 것으로 사료된다. Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which requlates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of demineralized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using demineralized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/10% FBS at the 37℃, 5% CO_2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCl(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/ml PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of 1 × 10 exp (5) cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows: The application of PDGF-BB for 1, 2 min slightyly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominetly increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingval fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.

[논문]토사터널 굴착시 발생하는 구조물의 부등침하 억제용 마이크로파일의 효과에 관한 연구

박진우,권정근,임종철 釜山大學校生産技術硏究所 2007 生産技術硏究所論文集 Vol.66 No.-

도심지에서 건설되는 각종 터널 시공시 현장여건 동 그 특수성에 따라 지중 및 지상에 존재하는 각종 인접구조물에 대한 안정성 확보가 필수적인 요건으로 대두되고 있다. 따라서 이같은 문제를 극복하면서 깊은 기초로서의 역학적 특성을 가지며, 현장의 적용성 및 시공성/ 경제성 등 여러 가지 장점을 가진 마이크로파일을 적용함에 있어서,선행연구에서 규명되어진 역학적 특성을 통해 파일의 적절한 보강형식과 현장의 적용성 및 보강의 효율성을 높이기 위한 수치해석을 실시하고/ 터널 인접구조물의 부등침하 안정검토 해석결과와 현장여건을 고려한 마이크로파일의 보강각도와 길이에 대한 효율적인 보강대책을 제시 하고자 한다.

폴 오스터의 『우연의 음악』에 나타난 정의로운 우연

박진우 慶熙大學校 大學院 2008 高凰論集 Vol.43 No.-

最近 5年間의 本院 X-線 治療患者의 統計的 考察

朴鎭雨 慶北大學校 醫科大學 1961 慶北醫大誌 Vol.3 No.1

1. Total number of the X-ray therapy patients treated at this hospital during 5years from 1955 to 1959 was 714 patients and this was equivalent to 0.7% of total out-patients of the hospital. 2. Geographic distribution of the X-ray therapy patients shows that the most patients(84.6%) were from the Kyungpuk Province. 3. Number of the X-ray therapy patients who discontinued the therapy with unknown reason was equivalent to 25% of total X-ray therapy patients. 4. Diagnosis was uncertain in 49 patients and these were subtracted from total number of the patients. Out of remaining 665 patients, there were 370 patients(55.6%) of benign disease and 295 patients(44.4%) of malignant tumor. 5. Majority of the benign disease patients was skin disease and 147 patients(39.7%) had eczema, and 50 patients(13.5%) had lichen simplex chronicus. 6. The benign diseases were most frequently occured between the age of 21 and 30, and 55.2% of the patients were before the age of 30. We have to pay attention that this is important in view of somatic and genetic radiation hazards. 7. Carcinoma was most frequent malignant tumor and 230 carcinoma patients (78.0% of the malignant tumor) were irradiated. The uterine cervix was most frequent site of carcinoma we treated and 94 patients(40.8% of the carcinoma) were irradiated.