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산처리(酸處理) 조건(條件)이 한천(寒天)의 수율(收率) 및 성질(性質)에 미치는 영향(影響)
박영이,이철,양한철,Park, Young-Yi,Lee, Chul,Yang, Han-Chul 한국식품과학회 1985 한국식품과학회지 Vol.17 No.5
참우뭇가사리 (Gelidium amansii sp.)를 원예(原藻)로 하여 추출조건( 抽出條件)(산(酸)의 종류(種類)와 농도(濃度) 및 추출시간(抽出時間) 등)에 따른 한천(寒天)의 수율(收率)과 이화학적성질(理化學的性質)을 분석(分析)하였으며, 조건별(條件別)로 추출(抽出)된 한천(寒天)에 대하여 agarose와 agaropectin의 비율(比率)을 측정(測定) 검토(檢討)하였다. 자숙중(煮熟中)에 염산(鹽酸), 황산(黃酸) 및 초산(酢酸)을 가했을 때, 추출(抽出)된 한천(寒天)의 수률(收率)과 젤리강도를 비교(比較)한 결과(結果) 염산(鹽酸)을 가하여 추출(抽出)한 것이 가장 우수(優秀)하였으며 산(酸)의 알맞은농도(濃度)는 0.005-0.01N HCI의 범위임을 알았다. 산(酸)을 첨가하지 않고 추출(抽出)한 대조구(對照區)의 수율(收率)과 젤리강도는 13.3%, $306g/cm^2$였다. 산(酸)의 종류(種類)와 농도별(濃度別)로 가장 높은 수율(收率)을 보인 추출조건(抽出條件)은 0.007N HCI의 38.7%, 0.007N $H_2SO_4$의 40.5%. 0.05N $CH_2 COOH의 42.4%였다. 그리고 가장 높은 젤리강도를 보인 추출조건(抽出條件)은 0.005N HCI일 때의 $511g/cm^2$, $0.005N\;CH_2 COOH일 때의 $463g/cm^2$, 0.01M $CH_2COOH$ 일때의 $436g/cm^2$였다. 그리고 구(對照區)와 젤리강도 및 수율(收率)이 가장 높게 나타난 산처리추출(酸處理抽出)된 한천중(寒天中) agarose의 함량이 가장 많은 것은 젤리강도가 가장 높은 0.005N HCI 처리구한천(處理區寒天)이었으며, 이때 성분(成分)과 황산기(黃酸基)의 함량은 가장 적었다. Agar was prepared from domestic agarophyte (Gelidium amansii) by a process of acid extraction. Optimal conditions in the acid treatment of the seaweed and the gelling properties of the agar thus prepared were investigated. The yield and gel strength of the control (the agar extracted with distilled water) showed 13,3% and 306g/$cm^2$, respectively. The yield of 38.7% was obtained when agar was extracted with 0.007N Hydorchloric acid while the gel strength of agar,511g/$cm^2$ was observed with the agar extracted with 0.005N Hydorchloric acid solution. The agar extracted with hydrochloric acid solution showed excellent properties of agar at the acid concentration range 0.005N-0.01N The cooking time of 60 min. was found to be effective to the yield and also to the gelling property of the agar and the cooking longer than 60 min. was proved to be destructive to agar. In general, yield and gelling properties of the agar obtained showed a close relation to the acid concentration and cooking time, but no consistent influence on the contents of ash and sulfur trioxide.
자궁경부암으로의 진행에 따른 vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs)의 발현
유선영 ( Sun Young Yu ),조용 ( Yong Cho ),임소이 ( So Yi Lim ),박영한 ( Young Han Park ),이용우 ( Yong Woo Lee ),최영희 ( Young Hee Choi ),노의선 ( Eu Sun Ro ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.5
Objective: To evaluate the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) in the progression of cervical cancer. Methods: A total 87 specimens of uterine cervix, representing 6 normal epithelium, 11 intraepithelial neoplasm, 25 cervical carcioma in situ (CIS), 19 cervical microinvasive carcinoma and 26 cervical invasive carcinoma, were evaluated. All specimens were prepared with tissue array method which can allow evaluation many specimens in one slide. The protein expressions of VEGF and MMPs (MMP-2, MMP-9) were evaluated by immunohistochemical staining. Results: The protein expressions of VEGF and MMP-2 were increased significantly with the progression of cervical cancer (p<0.05), but there was no statistically significant difference in MMP-9 expression (p>0.05). The VEGF and MMP-2 expressions revealed positive correlations with the progression of cervical cancer. Conclusions: The VEGF, MMP-2 in each or together may play a role in the progression of cervical cancer.
극지식물 Silene acaulis subsp. arctica의 유근 유래 캘러스로부터 다신초 유도와 기내 증식
서효원,이정윤,박영은,강성호,정호성,김지희,Seo, Hyo-Won,Yi, Jung-Yoon,Park, Young-Eun,Kang, Sung-Ho,Chung, Ho-Sung,Kim, Ji-Hee 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.4
고위도 북극지역에 분포하는 고등식물인 Silene aoaulis subsp. arctica (Caryophyllaceae)의 유근에서 유도된 캘러스로 부터 다신초를 재분화시키는 방법을 통하여 이 식물의 효율적인 기내 증식 방법을 확립하였다. 북극권 노르웨이령 Svalbard로부터 수집한 S. acaulis subsp. arctica의 종자를 발아시키고 0.25mg/L 2,4-D와 1mg/L $GA_3$가 포함된 고체 MS 배지상에서 $10{\pm}1^{\circ}C$와 $23{\pm}1^{\circ}C$ 온도조건으로 발아된 종자의 유근으로 부터 캘러스를 유도하였다. 캘러스가 형성된 2주 후부터는 0.25mg/L BA와 0.05mg/L NAA가 포함된 MS 배지에서 재분화가 효율적으로 이루어졌다. 재분화된 다신초들의 총 생체량 증가는 $23{\pm}1^{\circ}C$의 온도와 1/2 MS 배지에서 가장 높은 것으로 나타났다. 기내에서 다신초로 재분화된 식물체는 인공 상토에서 정상적인 식물체로 성장하였다. We describe here an efficient in vitro propagation method of Silene acaulis subsp. arctica (Caryophyllaceae), one of the higher arctic angiosperms, through the multiple shoot regeneration after callus induction from the radicle. The seeds of S. acaulis subsp. arctica collected from Svalbard, the Norwegian Arctic, were germinated and calli were induced from the radicle on solid MS media supplemented with 0.25mg/L 2,4-D and 1mg/L $GA_3$ at both $10{\pm}1^{\circ}C\;and\;23{\pm}1^{\circ}C$ Two weeks after callus induction, the multiple shoots were efficiently regenerated on the MS media supplemented with 0.25 g/L BA and 0.05mg/L HPh. The total biomass increment of regenerated shoots increased most efficiently of S. acaulis subsp. afctica was showed the maximum efficiency in at $23{\pm}1^{\circ}C$ on 1/2 MS salt strength. The multiple regenerated plantlets of S. acaulis subsp. arctics were grown to normal plants on soil.
한국 분리주 감자 잎말림 바이러스 (PLRV) 외피 단백질 유전자의 클로닝 및 감자 내 도입
서효원,이정윤,박영은,조지홍,함영일,조현묵,Seo Hyo-Won,Yi Jung-Yoon,Park Young-Eun,Cho Ji-Hong,Hahm Young-Il,Cho Hyun-Mook 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4
한국 분리주 감자잎말림바이러스 (PLRV)로부터 627bp 크기의 외피단백질 유전자의 ORF (AF296280)를 분리하여 장려품종 감자인 '수미'를 형질전환 하였다. 17계통의 형질전환체를 선발하여 온실과 포장에서 5세대를 증식하면서 PLRV에 대한 저항성이 큰 5계통을 선발 하였다. 도입된 유전자들의 유전적인 안정성을 확인하기위해 PCR, Southern, 그리고 northern blot 분석을 수행하였다. 또한 클론으로 증식된 형질전환 감자들의 특성과 저항성도 검정하였다. 형질전환된 감자들에서 PLRV의 외피단백질 유전자는 안전적으로 발현되며 저항성을 유지하였으며, 유사도가 비교적 낮은 감자 바이러스 Y (PVY)에는 저항성을 나타내지 않았다. 따라서 이러한 저항성은 도입된 유전자와 유사성도가 높은 바이러스에 저항성을 나타내는 homology dependent gene silencing으로 판단되었다. 유망계통 형질전환 감자 계통들의 포장평가를 통해 PLRV에 대한 저항성을 제외한 주요한 농업적 특성과 식물학적 특성은 형질전환 되지 않은 감자와 큰 차이를 보이지 않았다. The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV.
제초제 저항성 형질전환 감자의 농업적 특성, 영양 성분 및 해충 반응성 평가
안순영(Soon-Young Ahn),조광수(Kwang-Soo Cho),서효원(Hyeo-Won Seo),이정윤(Jeong-Yoon Yi),배신철(Shin-Cheol Bae),조지홍(Ji-Hong Cho),박영은(Young-Eun Park),김주일(Ju-Il Kim),김현준(Hyun-Jun Kim),조현묵(Hyun-Mook Cho) 한국원예학회 2011 원예과학기술지 Vol.29 No.3
The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.