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박선행,성윤영,장설,이시우,김홍준,김호경,Park, Sun Haeng,Sung, Yoon-Young,Jang, Seol,Lee, Siwoo,Kim, Hong Jun,Kim, Ho Kyoung 대한예방한의학회 2016 대한예방한의학회지 Vol.20 No.3
Objectives : Mibyeong is a korea medicine have original concept of the disease. However, no previous report has effect of mibyeong herbal medicine in fatigue types of mibyeong. This study investigated the question of whether Dang Gui Bo Hyul-tang(DGBHT) of effect on fatigue types of Mibyeong. Methods : C57BL/6 mice were randomaly divided into three group (n=10). The mice were then group (1) Nocontrol, (2) Restraunt stress(veh), (3) Dang Gui Bo Hyul-tang 200mg/kg. The administered Dang Gui Bo Hyul-tang 200mg/kg or distilled water (orally) 1 hr prior to daily exposure to repeated restraint stress (2 h) for 15 days. The performed behavior test (Mechanical hyperalgesia test,, Open-field test, Forced swimming test, Sucrose preference test and immunostaining and biochemical measured in serum. Results : Stress fatigue induced mices significantly increased lethargic, hyperalgesia through behavior test (Mechanical hyperalgesia test (decrease 43%), Open-field test ($4,809{\pm} 226.13cm$ vs. $3121{\pm}226.64cm$), Forced swimming test ($11.45{\pm}3.96$ vs. $79.10{\pm}8.12sec$), Sucrose preference test (decrease 58%)). In addition, chronic fatigue model evidently increased corticosterone level ($122.54{\pm}18.88$ vs. $186.94{\pm}18.26ng/ml$), AST level ($46.22{\pm}3.23$ vs. $31.40{\pm}3.86U/L$), ALT level ($38.78{\pm}5.72$ vs. $17.60{\pm}1.30$), liver necrosis, lateral ventricle size. These alterations were significantly ameliorated by DGBHT. DGBHT significantly attend the elevated serum concentrations of corticosterone ($155.90{\pm}6.29ng/ml$), AST ($31.40{\pm}3.86U/L$), ALT ($17.60{\pm}1.30U/L$). Moreover, DGBHT improved lethargic, hyperalgesia when compared the stress fatigue (Mechanical hyperalgesia test (improve 28%), Open-field test ($4,038{\pm}615.81cm$), Forced swimming test ($7.56{\pm}1.88sec$), Sucrose preference test (increase 21%) Conclusions : Theses result suggest that DGBHT have improved lethargic, hyperalgesia and fatigue-associated hormone and liver protective on stress fatigue model. It will be necessary to research to present evidences on benefits and effects of Korean medical treatment for Mibyeong through clinical researches based on benefits and effects of those animal models.
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Sun Haeng Park(박선행),Ji Hyun Kim(김지현),Sun Sik Bae(배순식),Ki Whan Hong(홍기환),Byung Tae Choi(최병태),Hwa Kyoung Shin(신화경) 한국생명과학회 2011 생명과학회지 Vol.21 No.5
Amyloid β (Aβ)의 신경독성은 알츠하이머병의 주된 원인이 되고 이러한 신경독성은 일련의 신경세포 사멸반응에 의해 일어난다고 알려져 있다. 본 연구에서는 알츠하이머병의 실험모델로 mouse primary neuronal cell에 Aβ25-35를 처리하여 세포독성을 유도하는 세포실험모델과 C57BL/6J mouse 뇌실에 Aβ25-35를 주입하여 인지장애를 일으키는 동물실험모델을 이용하여 phosphodiesterase Ⅲ 억제제인 cilostazol의 신경보호 효과에 대해 조사하였다. Aβ25-35를 신경세포에 처리하면 세포생존율이 감소되었고, 세포사멸이 일어난 세포의 수도 증가되었다. 이러한 Aβ25-35에 의한 세포독성이 cilostazol처리에 의해 회복되었으며, peroxisome proliferator-activated receptor (PPAR)-γ 항진제인 rosiglitazone 또한 동일한 회복효과를 나타내었다. Cilostazol과 rosiglitazone에 의한 이러한 회복효과가 PPAR-γ 길항제인 GW9662에 의해 다시 억제되는 결과를 통해 cilostazol의 효과는 PPAR-γ가 매개하는 신호전달이 관여함을 알 수 있었다. 직접 PPAR-γ 활성화 정도를 측정한 결과, Aβ25-35 처리에 의해 감소된 PPAR-γ 활성화 정도가 cilostazol과 rosiglitazone에 의해 증가함을 관찰할 수 있었고, 이는 GW9662에 의해 다시 억제됨을 확인하였다. 게다가, cilostazol은 세포사멸이 일어난 세포의 수와 세포사멸 조절단백질인 Bax/Bcl-2의 비율도 감소시켰다. Cilostazol (20 ㎎/㎏, 구강투여)을 C57BL/6J mice 뇌실에 Aβ25-35를 주입하기 2주 동안 전처리하고, Aβ25-35 주입 후 4주 동안 처리하면, 기억력과 학습능력을 증진시킨다는 결과를 water maze 실험을 통해 알 수 있었으며, rosiglitazone (10 ㎎/㎏)을 먹인 동물에서도 동일한 결과를 얻을 수 있었다. 본 연구를 통해서 cilostazol이 PPAR-γ 활성화를 통해 Aβ25-35로 인한 신경세포 손상과 세포사멸을 약화시켜, 신경세포의 생존을 증진시키고, 알츠하이머에서 인지장애를 개선할 것으로 생각된다. 따라서, phosphodiesterase Ⅲ 억제제인 cilostazol은 알츠하이머 질병 치료에 새로운 전략이 될 수 있을 것이다. The neurotoxicity of aggregated amyloid β (Aβ) has been implicated as a critical cause in the pathogenesis of Alzheimer’s disease (AD). It can cause neurotoxicity in AD by evoking a cascade of apoptosis to neuron. Here, we investigated the neuroprotective effects of cilostazol, which acts as a phosphodiesterase Ⅲ inhibitor, on Aβ25-35-induced cytotoxicity in mouse neuronal cells and cognitive decline in the C57BL/6J AD mouse model via peroxisome proliferator-activated receptor (PPAR)-γ activation. Aβ25-35 significantly reduced cell viability and increased the number of apoptotic-like cells. Cilostazol treatment recovered cells from Aβ-induced cell death as well as rosiglitazone, a PPAR-γ activator. These effects were suppressed by GW9662, an antagonist of PPAR-γ activity, indicative of a PPAR-γ -mediated signaling. In addition, cilostazol and rosiglitazone also restored PPAR-γ activity levels that had been altered as a result of Aβ25-35 treatment, which were antagonized by GW9662. Furthermore, cilostazol also markedly decreased the number of apoptotic-like cells and decreased the Bax/Bcl-2 ratio. Intracerebroventricular injection of Aβ25-35 in C57BL/6J mice resulted in impaired cognitive function. Oral administration of cilostazol (20 ㎎/㎏) for 2 weeks before Aβ25-35 injection and once a day for 4 weeks post-surgery almost completely prevented the Aβ25-35-induced cognitive deficits, as did rosiglitazone. Taken together, our findings suggest that cilostazol could attenuate Aβ25-35-induced neuronal cell injury and apoptosis as well as promote the survival of neuronal cells, subsequently improving cognitive decline in AD, partly because of PPAR-γ activation. The phosphodiesterase Ⅲ inhibitor cilostazol may be the basis of a novel strategy for the therapy of AD.
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정우양,박선행 한국목재공학회 1999 목재공학 Vol.27 No.3
European spruce and maple were to be estimated as raw material of violin family instruments with linear modal analysis to provide the information data for the design of their bodies. Wood specimens cut in different direction were excited by impact hammer to measure the resonant frequency with typical vibrational modes. In spite of lower density than maple, European spruce showed the excellent acoustical properties with higher resonant frequency. And edge-grained spruce had more even frequencies than flat-grained ones to be more acceptable as front plate of violin. Resonant frequency was positively correlated with wood density of each specimen and the coefficients of edge-grained specimens were higher than those of flat-grained specimens of both wood species.
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