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사료 중 단백질 수준 및 CLA (Conjugated Linoleic Acid) 첨가가 비육돈의 생산성 및 육질특성에 미치는 영향
문홍길 ( Hong Kil Moon ),이성대 ( Sung Dae Lee ),정현정 ( Hyun Jung Jung ),김영화 ( Young Hwa Kim ),박준철 ( Jun Cheol Park ),지상윤 ( Sang Yun Ji ),김종대 ( Chong Dae Kim ),권오섭 ( Oh Sub Kwon ),김인철 ( In Cheol Kim ) 한국동물자원과학회 2008 한국축산학회지 Vol.50 No.5
Cu-Soy Proteinate(Cu-SP)와 Herbal Mixture(HBM)의 급여가 육계의 생산성, 소장 내 미생물 균총 및 면역체계에 미치는 영향
김찬호,신광석,강환구,김지혁,황보종,최희철,문홍길,백인기,방한태,Kim, Chan Ho,Shin, Kwang Suk,Kang, Hwan Ku,Kim, Ji Hyuk,Hwangbo, Jong,Choi, Hee Cheol,Moon, Hong Kil,Paik, In Kee,Bang, Han Tae 한국가금학회 2014 韓國家禽學會誌 Vol.41 No.3
본 실험에서는 Cu-soy proteinate(Cu-SP)와 Herb-Mix(HBM)을 단독 또는 혼합 급여가 육계의 생산성, 혈액 성상, 면역 및 분내 미생물에 미치는 영향을 조사하기 위해 실시하였다. 육계(Ross308) 1,000수를 공시하여 5처리 4반복으로 반복 당 50수씩 완전 임의 배치하여, 자유 섭식하게 하였으며, 35일간 전기(0~21일), 후기(22~35일)로 나누어 실시하였다. 처리구들은 대조구(Control : negative control), 항생제구(Antibiotics : avilamycin 6 ppm 첨가구), Cu-SP(Cu-soy proteinate로 Cu 100 ppm 첨가구), HBM(지황 10%, 산약 10%, 당귀 20%, 오미자 5%, 감초 5%, 천궁 10%) 0.15% 첨가구, Cu-SP + HBM(Cu-soy proteinate로 Cu 100 ppm 첨가구 + HBM 0.15%) 첨가구 총 5 처리구였다. 35일의 사양 시험 결과, 전기 기간(0~21일)은 증체량, 사료 섭취량, 사료 요구량, 폐사율은 처리구 간에 유의적인 차이는 나타나지 않았다. 반면, 후기 기간(22~35일)에 사료 섭취량과 사료 요구량은 대조구가 유의적으로(P<0.05) 높았다. 전 기간(0~35일)에 증체량은 첨가구들이 유의적으로(P<0.05) 높았다. 반면, 사료 섭취량, 사료 요구량은 첨가구들이 유의적으로(P<0.05) 낮았다. 림프구(LY)는 Cu-SP + HBM구가 유의적으로(P<0.05) 높았으며, HE : LY 비율은 대조구가 유의적으로(P<0.05) 가장 높았다. 혈장 IgG 함량은 HBM 첨가구들이 유의적으로(P<0.05) 높았다. 혈장 IgA 함량은 처리구 간에 유의적인 차이는 나타나지 않았다. 유해균인 Cl. Perfringens는 대조구가 유의적으로(P<0.05) 높았으며, E. coli와 Lactobacillus는 처리구 간에 유의적인 차이는 나타나지 않았다. 결론적으로 Cu-SP와 HBM의 단독 급여나 혼합 급여는 둘 다 생산성을 증가시키며, 면역성상을 개선시키는 역할을 한다. 또한 소장 내 유해 미생물을 억제하는 긍정적인 효과를 보여주었다. The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, intestinal microflora, and immune response in broiler. A total 1,000 1-d old ROSS 308 (initial $BW=41{\pm}0.38g$) were randomly allotted to 1 of 5 dietary treatments with 4 replicates during d 35 of the feeding trial. Dietary included: (1) Control : control diet, (2) Antibiotics : control diet + Avilamycin 6 ppm, (3) Cu-SP : control diet + 100 ppm Cu-soy proteinate, (4) HBM : control diet + 0.15% herbal mixture, (5) Cu-SP+HBM : control diet + 100 ppm Cu-soy proteinate + 0.2% herbal mixture. Two-phase feeding program with a starter diet from d 0 to 21, and a finisher diet from d 22 to 35 was used in the experiment. Within each phase, a diet was formulated to meet or exceed NRC requirements of broilers for macro- and micronutrients. The diet and water were available ad libitum. Result indicated that during d 22 to 35 and over all periods of the experiment, feed intake and feed conversion ratio were greater (P<0.05) for other treatment than control. Significant differences were found in lymphocyte (LY), and stress indicator (HE:LY ratio). Lymphocyte was greater (P<0.05) for Cu-SP + HBM treatment than control. However, stress indicator (HE:LY ratio) were greater (P<0.05) for control than Cu-SP + HBM treatment. The plasma IgG was higher (P<0.05) in the antibiotics, HBM, and Cu-SP+HBM treatments groups compared with control. The population of Clostridium perfringens in the antibiotics, Cu-SP, HBM, Cu-SP + HBM treatment groups were lower (P<0.05) than those control. These result suggested that dietary copper-soy proteinate or herbal mixture may be used as an alternative to antibiotics to improve growth performance, and intestinal health of birds.
매실과 홍삼 부산물이 스트레스에 대한 육계 생체 반응에 미치는 영향
봉미희(Mi Hee Bong),지상윤(Sang Yoon Ji),박준철(Jun Cheol Park),문홍길(Hong Kil Moon),이상철(Sang Cheul Lee),이준헌(Jun Heon Lee),홍준기(Joon Ki Hong) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.3
This study was conducted to test the efficacy of plum (Prunus mume) and red ginseng (Panax ginseng C.A Meyer) marc as stress inhibitors under heat stress and lipopolysaccharide (LPS) challenge in broilers by investigating their effects on blood biochemical parameters, immunoglobulin concentration and splenic cytokine mRNA expressions. A total of one hundred ninety-two 1-d-old male broiler chicks (Ross 308) were divided into 2 stress conditions (heat and LPS) experiments. Each experiment was divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, 25 ppm β-glucan), PM (1% plum marc) and RGM (3% red ginseng marc) treatments were administered with respective substance through water supplementation. During heat stress, The Ca/Mg ratio in PM and RGM was significantly decreased in comparison with that of NC (P<0.05). The immunoglobulin M was significantly lower in PM than in NC (P<0.05). Expression patterns of splenic cytokine mRNAs (IL-1, IL-2 and IL-6) were similar over the treatment. Expression rates of IL-1 and IL-2 in PM were significantly decreased in comparison with NC. Also, expression rates of IL-1, IL-2 and IL-6 were significantly lower in RGM than in NC (P<0.05). In conclusion, the dietary supplementation of plum and red ginseng marc improved coping ability to heat stress by preventing Ca/Mg ratio increment and by inhibiting inflammatory response in broiler chicks. However, it is necessary to determine optimal dietary level of red ginseng marc for improving growth performances in broiler chickens. These results suggest the possibility that plum and red ginseng marc could be used as the stress inhibitor under heat stress and inflammatory response in broiler chicks.
단위가축영양 : 육계에서 구멍갈파래의 항산화 및 면역조절 효과
홍준기 ( Joon Ki Hong ),봉미희 ( Mi Hee Bong ),박준철 ( Jun Cheol Park ),문홍길 ( Hong Kil Moon ),김동욱 ( Dong Wook Kim ),이상철 ( Sang Cheul Lee ),이준헌 ( Jun Heon Lee ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.5
This study was conducted to test the efficacy of Ulva pertusa kjellman as immunomodulators under lipopolysaccharide(LPS) challenge in broilers by investigating their effects on serum superoxide dismutase(SOD) like ability, immunoglobulin concentration, and splenic cytokine mRNA expression. A total of ninety six1-d-old male broiler chicks(Ross 308) were divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC(negative control, no immune substances), PC(positive control, β-glucan 25 ppm), Ulva P(Ulva pertusa kjellman Powder 3%), and Ulva E(Extract form Ulva pertusa kjellman 0.3%) were added in feed with respective substance. Heparinized venous blood and spleens were collected from five birds per dietary treatment at 5 wk of age. The SOD like activities in Ulva P and Ulva E were significantly increased in comparison with other groups(P<0.05). The immunoglobulin M content was lower in the Ulva E than other groups(P<0.05). Expression patterns of splenic cytokine mRNA in the IL-1β, IL-2 and IL-6 were similar. Expression rate of IL-1β, IL-2 and IL-6 in Ulva pertusa kjellman(Ulva P, Ulva E) were decreased(P<0.05) in comparison to other groups. Expression rate of IL-1β, IL-2 and IL-6 were significantly lower in groups treated with Ulva E than Ulva P. In conclusion, dietary supplementation of Ulva pertusa kjellman improved SOD like activity and affect immune system by inhibiting inflammatory response in broiler chicks. In addition, it is more effective to use Ulva pertusa kjellman extract than powder for immunomodulatory function. These results suggest the possibility that Ulva pertusa kjellman could be used as the immunomodulator for inflammatory response in broiler chicks.