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Studies on the function of DNA Polymerase β Using Transgenic Drosophila
이원호,유미애,류재련,하혜영 한국유전학회 1994 Genes & Genomics Vol.16 No.3
The biological function of DNA polymerase β in the whole organism was examined by using a transgenic Drosophila bearing chimeric gene consisting of a promoter region of Drosophila actin 5C gene and rat DNA polymerase β cDNA. Southern analysis of genomic DNA of transgenic flies and transmission pattern of p[w^+;polβ] indicated that a single copy of p[w^+;polβ] was integrated into each transgenic strain. To elucidate the role of DNA polymerase β in DNA repair, the sensitivity of transgenic strain to killing after treatment of MMC, MMS and UV were compared with that of host strain. The sensitivity of transgenic strain to mitotic recombination induced by MMC or MMS were also compared with host strain. The present results suggest that rat DNA polymerase β expressed in transgenic flies play an important role at least in the generation process of mitotic recombination.
Yoo, Mi-Ae,LEE, Won-Ho,HA, Hye-Yeong,RYU, Jae-Ryeon,YAMAGUCHI, Masamitsu,FUJIKAWA, Kazuo,MATSUKAGE, Akio,KONDO, Sohei,NISHIDA, Yasuyoshi 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-
DNA polymerase β(pol β)cDNA of rat fused to an enhamcer-promoter ergion plus a poly(A) signal sequence of actin 5C gene of Drosophila(abbreviated polβ) was transferred to the Drosophila genome. Three of four constructed transgenic strains possessing transgene polβ on different chromosomes were studied. Levels of the polβ transcript and those of the polymerization activity of polβ were markedly elevated in cultured cells trans fected with polβ-bearing vectors as well as in embryos of the transgenic strains. The popular idea that DNA polymerase β participates in DNA repair was not supported by the observation that a pair of a normal and a polβ strain, and the other pair of a mei-9 mei-41(DNA-repair deficient double mutations) strain and a polβmei-9 mei-41 strain, showed no difference in survial within each pair after treatment with ultraviolet light, methylmethane sulfonate and mitomycin C. The other idea that DNA polymerase β participates in recombination was supported by the findings that spontaneous freauency of recombination, either meiotic or mitotic, is significantly higher in a transgenic polβstrain than in a non-transgenic strain. The enhanced recomdination frequency in the polβ strain may, however, reflect an indirect effect of over-produced polβ prteins on chromosomal stability. Whatever the direct effect of rat polβis, the transgenic polβ flies will be useful for study of the physiological role of polβ and the mechanism of recombination.
Clonidine Patch 제의 효능시험 혈압강하작용
고광호(Kwang Ho Ko),류재련(Jea Ryeon Ryu),박우일(Woo Yle Park),정재훈(Jae Hoon Cheong),신찬영(Chan Young Shin) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2
Clonidine, an antihypertensive drug, stimulates postsynaptic alpha-2 adrenergic receptors in the central nervous system and lowers arterial pressure through the effects on both cardiac output and peripheral resistance. However, many patients experience that sedation and xerostomia occur upon oral administration of clonidine. These side effects are due to high plasma peak concentration and can be avoided when clonidine is given transdermally. In this study, we tested the antihypertensive effects of trandermal administration of clonidine patch on spontaneously hypertensive rat (SHR) which is a model animal for human essential hyperkension. Forty eight SHR (male) were divided into six groups according to the dose levels, respectively. After transdemal administration of clonidine patch of each dose, systolic blood pressure and heart rate were measured. Clonidine patch produced maximal antihypertensive and bradycardiac effects 48 hrs after administration and antihypertensive effects showed dose-dependency. We suggest that antihypertensive effects of clonidine patch are similar to those of orally given clonidine and clonidine patch can be used instead of clonidine tablet.
김영신,이원호,유미애,류재련,김형인,이희정 한국유전학회 1996 Genes & Genomics Vol.18 No.2
D-raf, a Drosopihila hornolog of Raf-1, functions as a signal transducer in cell proliferation and differentiation. The reporter plasmid p5'-1103 Draf-lacZ fused the Draf gene regulatory region (-1103∼+300 with respect to the translation initiation site) with the lacZ gene in a P-element vector was constructed. Expression of the Draf-lacZ fusion gene was demonstrated in Kc cells by transient expression assay. Transgenic lines bearing Draf-lacZ fusion gene were established by P-element mediated transformation with the reporter plasmid. Spatial pattern of the Draf-lacZ expression in transgenic embryos and ovaries was similar to the distribution of the endogenous D-raf transcripts examined by in situ hybridization in previous study. We first report the expression of D-raf gene in larval fat body analogous to the vertebrate liver and in testis and anterior ejaculatory duct of adult male. And our results show that the regulatory region is sufficient for expression of the D-raf gene. Draf-lacZ transgenic flies obtained in this study promise to be useful for studying multiple roles and regulation of expression of D-raf gene.
최승혁,ALISTARE ROBERT SADR,강지은,류재련,김준환,선웅,허성오 한국뇌신경과학회 2019 Experimental Neurobiology Vol.28 No.2
Rheb (Ras homolog enriched in the brain) is a small GTPase protein that plays an important role in cell signaling for development of the neocortex through modulation of mTORC1 (mammalian-target-of-rapamycin-complex-1) activity. mTORC1 is known to control various biological processes including axonal growth in forming complexes at the lysosomal membrane compartment. As such, anchoring of Rheb on the lysosomal membrane via the farnesylation of Rheb at its cysteine residue (C180) is required for its promotion of mTOR activity. To test the significance of Rheb farnesylation, we overexpressed a farnesylation mutant form of Rheb, Rheb C180S, in primary rat hippocampal neurons and also in mouse embryonic neurons using in utero electroporation. Interestingly, we found that Rheb C180S maintained promotional effect of axonal elongation similar to the wild-type Rheb in both test systems. On the other hand, Rheb C180S failed to exhibit the multiple axon-promoting effect which is found in wild-type Rheb. The levels of phospho-4EBP1, a downstream target of mTORC1, were surprisingly increased in Rheb C180S transfected neurons, despite the levels of phosphorylated mTOR being significantly decreased compared to control vector transfectants. A specific mTORC1 inhibitor, rapamycin, also could not completely abolish axon elongation characteristics of Rheb C180S in transfected cells. Our data suggests that Rheb in a non-membrane compartment can promote the axonal elongation via phosphorylation of 4EBP1 and through an mTORC1-independent pathway.
고광호(Kwang Ho Ko),조미정(Mi Jeong Jo),이장훈(Jang Hoon Lee),노민수(Min Su Noh),류재련(Jea Ryeon Ryu),이진화(Jin Hwa Lee),안재석(Jae Suk Ahn),태주호(Joo Ho Tai),민동선(Dong Sun Min),박병욱(Pyeong Uk Park),김기협(Key Hyup Kim) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.1
Anti-inflammatory and analgesic activities and skin irritation of piroxicam patch were investigated. Piroxicam patch increased the pain threshold in rat hind paw inflamed by carrageenan and inhibited writhing induced by acetic acid in mice. Piroxicam patch also inhibited the carrageenan-induced edema in rat hind paw as well as the increased vascular permeability induced by histamine in rats. In adjuvant arthritis of rats, piroxicam patch showed anti-inflammatory effects. Skin irritation of piroxicam patch was tested in Newzealand White rabbits and evaluated by Primary Irritation Index of Draize. The results from skin irritation test showed that piroxicam patch seemed practically non-irritating. The result from the present study indicates that piroxicam may be useful without serious side effects as anti-inflammatory analgesics in this patch form.