http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
인간 각막세포에서 국소 면역억제 인자 Indoleamine 2,3-dioxygenase의 발현
류양환,김재찬,Yang Hwan Ryu,M,S,Jae Chan Kim 대한안과학회 2007 대한안과학회지 Vol.48 No.8
Purpose: To identify the localization of indoleamine 2,3-dioxygenase (IDO) in human corneal cells and to evaluate its ability to act as a local immunosuppressive factor. Methods: The expression profile of IDO was obtained with RT-PCR and Western blot of in a primary culture of human corneal cells (fibroblasts, epithelial cells and endothelial cells). In order to investigate the immunosuppressive function of IDO, immune cells were cultured in a human corneal cell-conditioned medium, and their prolifleration was identified by the MTT assay. Moreover, apoptotic effects of IDO in immune cells treated with IFN-γ were also investigated with apoptosis ELISA. Results: Among the three different types of human corneal cells analyzed, mRNA and protein expression of IDO was observed only in human corneal fibroblasts. Immune cells cultured in a human corneal fibroblast-conditioned medium showed inhibited proliferation. Moreover, IFN-γ-induced expression of IDO significantly enhanced apoptotic ability in a dose-depandant manner. Conclusions: Our results suggest that human corneal fibroblasts are relatively immuno-resistant and that expression of IDO may be one of the factors involved in the immune tolerance observed in corneal grafts.
항녹내장 약제가 배양된 사람 결막상피세포에 미치는 영향
이정규,류양환,Jeong Kyu Lee,Yang Hwan Ryu 대한안과학회 2006 대한안과학회지 Vol.47 No.11
Purpose: To investigate the toxicity of a short-term application of timolol maleate, dorzolamide, and benzalkonium chloride (BAC) on human conjunctival epithelial cells in vitro. Methods: Chang‘s conjunctival epithelial cell line was treated for 5 min, 15 min, 30 min, and 60 min with various concentrations of timolol, dorzloamide, or BAC, and then examined 4 hrs or 24 hrs later. Cell viabilities were assessed by MTT assay. The expressions of various cytokines by timolol maleate, dorzolamide, and BAC treatment in human conjunctival epithelial cells were evaluated using ELISPOT. Results: BAC significantly decreased survival of conjunctival epithelial cells in a dose and time dependent manner compared with timolol and dorzolamide. Inflammatory cytokines, IL-6 and IL-8, were highly expressed in conjunctival epithelial cells treated with timolol, dorzolamide, and BAC. Conclusions: The present study suggests that increased expression of inflammatory markers, IL-6 and IL-8 might explain the ocular surface disorder in patients receiving antiglaucoma medication.
건성안에서의 항산화 및 염증 억제 기능성 복합식품의 효과
김재훈,김호영,류양환,전연숙,유인천,윤경철,김재찬,Jae Hoon Kim,Ho Young Kim,Yang Hwan Ryu,Yeoun Sook Chun,In Cheon You,Kyoung Chul Yoon,Jae Chan Kim 대한안과학회 2008 대한안과학회지 Vol.49 No.9
Purpose: To evaluate the effect of Optibiol®, which is composed of multiple anti-oxidative and anti-inflammatory dietary elements, on the symptoms, clinical manifestations, and expression of various inflammatory cytokines in dry eye patients. Methods: Patients suffering from dry eye were given Optibiol® for 3 months. They completed questionnaires regarding dry eye symptom and underwent slit lamp biomicroscopic examinations, tear film breakup times, Shirmer tests, and conjunctival fluorescein staining examinations on a monthly basis during the intake of Optibiol®. Sampling of serum and tears was conducted at baseline and 3 months after taking Optibiol®, and various inflammatory cytokines in the serum and tears were measured with multiplex bead immunoassay. Result: Sixty-three patients were included in this study. After taking Optibiol®, the dry eye symptoms, Schirmer scores, tear film breakup times, and conjunctival staining scores (Oxford scale) showed significant improvement, and the expression of most inflammatory cytokines had decreased: in particular, IL-1β and MIP-1β in serum, and IL-17 and MIP-1β in tears were significantly lower. Conclusions: Optibiol®, an anti-oxidative and anti-inflammatory functional dietary supplement, is an effective dietary supplement in patients with dry eye syndrome. We posit that the decreased expression of inflammatory cytokines is an important mechanism in this effect. J Korean Ophthalmol Soc 2008;49(9):1397-1405
연구논문 : 인공피부모델 KeraSkin(TM) 피부자극성 시험 대체시험법의 실험실간 검증
문지영 ( Ji Young Moon ),이수현 ( Su Hyon Lee ),정행선 ( Haeng Sun Jung ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),임경민 ( Kyung Min Lim ),박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.2
Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “Keraskin(TM)” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and Keraskin(TM), but IL-1α levels of some test items different between EPI-200 and Keraskin(TM). We concluded that this Keraskin(TM) model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.
정행선 ( Haeng Sun Jung ),이수현 ( Su Hyon Lee ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),김채욱 ( Chae Wook Kim ),임경민 ( Kyung Min Lim ),손영숙 ( Young Sook Son ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
Alternative methods to the Draize eye irritation test, such as the BCOP, HET-CAM, ICE, and IRE, are used to evaluate the ocular irritation potential of cosmetic, livelihood articles or industrial chemicals. In order to improve the sensitivity and specificity of alternative eye irritation test, we developed a three-dimensional human corneal model that uses normal human corneal epithelial cells. Our corneal tissue model consists of normal human corneal epithelial cells on cell culture inserts at the air liquid interface, differentiating to form a stratified, squamous epithelium similar to that found in the human cornea, exhibiting in vivo like morphological and histological characteristics. In this study, two laboratories have tested 20 reference chemicals using the same study protocol. The results were compared to in vivo data as well as previously published data obtained in the other three-dimensional corneal model test. Although an overall accuracy of 85% was obtained (sensitivity=100% and specificity=67%), further experiments are required to confirm and validate these preliminary results.
인공피부모델 KeraSkin(TM) 피부자극성 시험 대체시험법의 실험실간 검증
문지영 ( Ji Young Moon ),이수현 ( Su Hyon Lee ),정행선 ( Haeng Sun Jung ),류양환 ( Yang Hwan Ryu ),정경미 ( Kyoung Mi Jung ),임경민 ( Kyung Min Lim ),박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.2
Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “Keraskin(TM)” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and Keraskin(TM), but IL-1α levels of some test items different between EPI-200 and Keraskin(TM). We concluded that this Keraskin(TM) model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.