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남궁슬기,성지혜,양진우,최용민,정헌상,이준수 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.9
Crosstalk between adipocytes and macrophages has been suggested to play a crucial role in metabolic disorders such as obesity, insulin resistance, and type 2 diabetes. The objective of this study was to evaluate the effect of nobiletin on the interaction between adipocytes and macrophages. The results showed that nobiletin significantly and dose-dependently inhibited the secretion of inflammatory mediators, such as nitric oxide (NO), tumor necrosis factor (TNF-α), and monocyte chemoattractant protein (MCP)-1, in a coculture of adipocytes and macrophages. The expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), in differentiated 3T3-L1 cells cocultured in transwell system was blocked by nobiletin. Nobiletin also downregulated the expression of inducible NO synthase in cocultured differentiated RAW264.7 cells. Furthermore, heme oxygenase-1 (HO-1) was significantly induced by nobiletin treatment in both cell types, and small interfering (si) RNA-mediated knockdown of HO-1 significantly recovered the inhibitory effects of nobiletin on the NO production in cocultured cells. These results suggest that nobiletin exerts anti-inflammatory effects on the crosstalk between adipocytes and macrophages by inducing HO-1. Nobiletin may have potential for the prevention of obesity-related metabolic diseases.
남궁슬기,최용민.김영화,이준수 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1
For the complete extraction of folates from food matrices, tri-enzyme(a-amylase, proteases, and conjugase) treatment is highly recommended. Currently, AOAC official method 2004.05 uses Lactobacillus casei ssp. Rhamnosus for a microbiological assay and the tri-enzyme treatment as an extraction method. However, the official method in Korean Food Standards Codex(KFSC) for the folate assay does not use the tri-enzyme extraction method. Therefore, the purpose of this study was to compare two extraction procedures, tri-enzyme treatment and KFSC extraction method. Folate values were 1022.3 ± 16.2 (perilla leaf), 1180.6 ± 38.0 (kale), 1260.2 ± 92.3 (vitamin vegetable), 1354.9 ± 70.3 (artemisia) by tri-enzyme treatment. However, folate values by KFSC extraction method were 105.0 ± 6.9 (perilla leaf), 221.9 ± 32.3(kale), 78.5 ± 1.8(vitamin vegetable), 214.4 ± 8.0 (artemisia). Also, folate values from tri- enzyme treatment were significantly higher than those from the KFSC method in all tested grain samples. In addition, the analytical validation parameters using tri-enzyme treatment were reliable and satisfactory. In conclusion, we suggest the use of tri-enzyme treatment for the microbiological assay of folate in vegetables and grains.
최슬기,신용환,남궁은,황성민,Xin Cong,Guangyan Yu,박경표 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.6
Transient receptor potential vanilloid subtype 1 (TRPV1) was originally found in sensory neurons. Recently, it has been reported that TRPV1 is expressed in salivary gland epithelial cells (SGEC). However, the physiological role of TRPV1 in salivary secretion remains to be elucidated. We foundthat TRPV1 is expressed in mouse and human submandibular glands (SMG) and HSG cells, originatedfrom human submandibular gland ducts at both mRNA and protein levels. However, capsaicin (CAP),TRPV1 agonist, had little effect on intracellular free calcium concentration ([Ca2+]i) in these cells,although carbachol consistently increased [Ca2+]i. Exposure of cells to high temperature (>43oC) oracidic bath solution (pH5.4) did not increase [Ca2+]i, either. We further examined the role of TRPV1in salivary secretion using TRPV1 knock-out mice. There was no significant difference in the pilocarpine(PILO)-induced salivary flow rate between wild-type and TRPV1 knock-out mice. Saliva flow rate alsoshowed insignificant change in the mice treated with PILO plus CAP compared with that in micetreated with PILO alone. Taken together, our results suggest that although TRPV1 is expressed inSGEC, it appears not to play any direct roles in saliva secretion via transcellular pathway.
Role of LPA and the Hippo pathway on apoptosis in salivary gland epithelial cells
황성민,진미홍,신용환,최슬기,남궁은,김민경,박문용,박경표 생화학분자생물학회 2014 Experimental and molecular medicine Vol.46 No.-
Lysophosphatidic acid (LPA) is a bioactive lysophospholipid involved in numerous physiological responses. However, the expression of LPA receptors and the role of the Hippo signaling pathway in epithelial cells have remained elusive. In this experiment, we studied the functional expression of LPA receptors and the associated signaling pathway using reverse transcriptase–PCR, microspectrofluorimetry, western blotting and immunocytochemistry in salivary gland epithelial cells. We found that LPA receptors are functionally expressed and involved in activating the Hippo pathway mediated by YAP/TAZ through Lats/Mob1 and RhoA/ROCK. Upregulation of YAP/TAZ-dependent target genes, including CTGF, ANKRD1 and CYR61, has also been observed in LPA-treated cells. In addition, based on data suggesting that tumor necrosis factor (TNF)-α induces cell apoptosis, LPA upregulates TNF-induced caspase-3 and cleaved Poly(ADP-ribose)polymerase (PARP). However, small interfering RNA treatment to Yes-associated protein (YAP) or transcriptional co-activator with a PDZ-binding motif (TAZ) significantly decreased TNF-α- and LPA-induced apoptosis, suggesting that YAP and TAZ modulate the apoptotic pathway in salivary epithelial cells.
tert-Butyl Hydroperoxide로 산화 스트레스가 유도된 HepG2 세포에서 말채나무 열수추출물의 항산화 활성
연성호(Seong Ho Yeon),함현미(Hyeonmi Ham),성지혜(Jeehye Sung),김영화(Younghwa Kim),남궁슬기(Seulgi Namkoong),정헌상(Heon-Sang Jeong),이준수(Junsoo Lee) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.10
말채나무 열수추출물의 항산화 활성을 알아보기 위해 HepG2 세포에 TBHP로 산화 스트레스를 유도한 뒤 말채나무 열수추출물의 세포 보호효과, ROS 생성억제, 지질과산화 억제 및 GSH 생성에 미치는 영향에 대해 살펴보았다. 말채나무 열수추출물은 HepG2 세포에 TBHP로 산화 스트레스를 유도한 뒤 나타나는 세포독성에 대해 농도 의존적으로 유의하게 세포 보호효과를 보였으며, ROS 생성과 과산화물에 대한 지표로서 측정한 MDA도 말채나무 열수추출물에 의해 효과적으로 억제되었다(P<0.05). 또한 항산화 성분으로 생체 내에서 산화 및 환원반응에 중요한 역할을 하며 항산화 효소인 GSH-Px, GST에 전자공여체로 작용하는 GSH의 생성촉진 효능에서도 말채나무 열수추출물은 산화 스트레스로 감소된 GSH의 생성을 농도 의존적으로 촉진시켰다(P<0.05). 산화 스트레스에 의해 활성이 증가된 항산화 효소(CAT, SOD, GSH-Px, GR)도 말채나무 열수추출물의 처리로 감소하는 경향을 보였다. 이러한 결과로 미루어 보아 말채나무 열수 추출물은 인체 내에서 질병과 노화를 일으키는 원인 물질인 활성산소에 대해 강한 항산화 활성을 나타냄에 따라 보다 다양한 형태의 소재로 활용될 수 있을 것으로 생각된다. The objective of this study was to investigate the effect of hot water extract from Cornus walteri Wanger(CWE) on tert-butyl hydroperoxide (TBHP)-induced oxidative stress in HepG2 cells. Generation of reactive oxygen species (ROS), concentrations of cellular lipid peroxidation products and reduced glutathione, and antioxidant enzyme activity were used as biomakers of cellular oxidative status. Cells pretreated with CWE (25~200μg/mL) showed an increased resistance to oxidative stress in a dose-dependent manner, as revealed by a higher percentage of surviving cells compared to control cells. ROS generation induced by TBHP was significantly reduced when cells were pretreated with 200 μg/mL CWE for 4 h. Pretreatment with CWE (5~50 μg/mL) prevented the decrease in reduced glutathione and the increase in malondialdehyde and ROS evoked by TBHP in HepG2 cells. Finally, CWE pretreatments prevented the significant increase of glutathione peroxidase, catalase, glutathione reductase, and superoxide dismutase activities induced by TBHP. These results show that CWE has significant protective ability against a TBHP-induced oxidative insult and that the modulation of antioxidant enzymes by CWE may have an important antioxidant effect on TBHP-induced oxidative stress in HepG2 cells.