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파프리카의 저장성 향상을 위한 플라즈마 살균 시스템 개발과 이의 효과 검정
김현승 ( Kim,H. S. ),고선민 ( S. M. Go ),정래동 ( R. D. Jeong ),권덕호 ( D. H Kwon ),박미리 ( M. R. Park ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.3
Paprika is an important agricultural export in Korea. However, paprika is not suitable for exporting over long distances as it does not store well. A major cause of damage to fresh agricultural produce, including paprika, is infection with pathogenic microorganisms that cause post-harvest diseases during storage and distribution. To address this problem, research is ongoing to develop a range of surface sterilization techniques. The recent confirmation of the sterilization effects of reactive oxygen species (ROS) generated from plasma discharge has attracted substantial research interest in applying the plasma technology to agriculture. Therefore, a plasma sterilization system was developed in this study to improve paprika storability. This system consists of an atmospheric-pressure plasma generator, a plasma controller, and a chamber for plasma treatment. To validate the system performance, the internal chamber temperature was measured; the volume of ozone, a strong oxidant that generates ROS, was also measured to compute the volume of all ROS generated through plasma discharge; based on this calculation, the concentration of ROS that can effectively deactivate microorganisms was identified. The sterilization effect of the present system was examined using Erwinia carotovora, the bacteria responsible for soft rot in paprika. Erwinia carotovora samples were treated with plasma for 30 and 60 s with 2.5 bar pressure and 500 W, 700 W, and 1000 W plasma power. The samples treated for 60 s showed a 100% sterilization effect. To then validate the effectiveness of the system in maintaining paprika freshness, paprika were treated at 1000 W plasma power for 30 and 60 s. As a result, browning was observed in the stem end of untreated paprika due to withering, whereas browning was delayed for 26 d in the stem end of plasma-treated paprika.
저온 대기압 플라즈마를 이용한 저장성병원균 Alternaria alternata 및 Botrytis cinerea 의 제어 효과
고선민 ( Go,S. M. ),김현승 ( H. S. Kim ),박미리 ( M. R. Park ),정래동 ( R. D. Jeong ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.3
Non-thermal atmospheric plasma (NTAP) has been shown to be effective for controlling postharvest fungi in vitro, but little is known about its mode of action, fungal response to NTAP, and the effect of its application on fresh produce. Non-thermal atmospheric plasma was evaluated for its in vitro and in vivo antifungal activity against Alternaria alternata and Botrytis cinerea on paprika. The treatment with NTAP treatment resulted in complete inhibition of mycelial growth of A. alternata, and B. cinerea, particularly at 1000 W for 90 s. The results of in vivo assays demonstrated that NTAP treatment for 90 s inhibited the growth of these fungal pathogens by approximately 55% (A. alternata) and 20% (B. cinerea). The color and hardness of paprika exhibited no significant changes during 7 days of storage after NTAP treatment. The mechanisms by which NTAP treatment decreased fungal growth on paprika were directly associated with the disruption of fungal cell membrane. These findings suggest that the application of NTAP is an effective antifungal treatment approach for preserving paprika.
김현승(H. S. Kim),이준상(J. S. Lee),조동현(D. H. Cho),강성웅(S. W. Kang),나태엽(T. Y. Na),장호(H. Jang) 한국트라이볼로지학회 2014 한국윤활학회지(윤활학회지) Vol.30 No.1
The temperature-dependent tribological properties of brake discs for a train were examined in this study. The discs were produced using heat-resistant alloy steel, which showed different thermal conductivity after the heat treatments. A commercial brake friction material was used to evaluate the friction effectiveness, and the friction tests were carried out using a 1/5 scale dynamometer under various initial braking temperature conditions. The results showed that the tribological property of the disc was strongly affected by the heat treatment schedule. At low temperatures (below 250℃), the friction coefficient increased as a function of disc temperature, indicating that frictional heat increased the adhesion between the disc and pad. In addition, fade was observed at high temperatures (above 250℃); it was pronounced in the case of the disc with low thermal conductivity. The different fade resistances observed in the discs with different heat treatment schedules appear to be influenced by microstructural changes such as carbide redistribution occurring during the heat treatments, which affected the thermal conductivity.
수박과실썩음병을 일으키는 Acidovorax citrulli와 Acidovorax valerianellae에 대한 살균제의 살균효과 연구
김현승 ( H S Kim ),엄용현 ( Y H Um ),김상우 ( S W Kim ),딜라즈야다브 ( D R Yadav ),마헤시아드히카리 ( M Adhikari ),이성찬 ( S C Lee ),이윤수 ( Y S Lee ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2015 강원 농업생명환경연구 Vol.27 No.3
Antimicrobial activities of nine chemical fungicides were tested against thirty one strains of Acidovorax spp.,causative agent of bacterial fruit blotch (BFB) disease on cucurbits. As a result, nine chemical fungicides showed antimicrobial activities. Fugicide C showed 99% inhibition rate of colony formation of 26 isolates of Acidovorax spp. Fungicides A, B, E, G and H also showed high inhibition rates. Fungicide B showed more than 99% growth inhibition rate against A. citrulli isolate 11-164. Howerver, it had narrow antibacterial spectrum. Fungicide A, E, G,H showed 93.18%, 93.13%, 95.79%, 95.18% inhibition rate against isolate 17000, 12-158, 13-211 and 17000,respectively. Therefore, this study suggests that fungicide B and C could be used for potential fungicides to control BFB pathogens. However, further study is necessary to confirm their effect in vivo.