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배추흰나비 (Pieris rapae L.)의 미세구조에 관한 연구 II. 난소(卵巢)의 발생(發生)과 난성숙(卵成熟)
김창환,김우갑,김지현,Kim, C.W.,Kim, W.K.,Kim, J.H. 한국현미경학회 1985 Applied microscopy Vol.15 No.1
A observation of the ovarian development and oogenesis of Pieris rapae Linne has been carried out during metamorphosis using stereo-microscope, light microscope and electron microscope. The results obtained through this experiment are as follows: 1. The ovarian development and vitellogenesis begin at the 3-day old pupa and the 6-day old pupa respectively, and the adult ovary right after their emergence contains a few mature eggs. 2. The species described above are further observed at six different stages in oogenesis, and the results are summarized as follows. 1) Pieris rapae has polytrophic ovarioles. The cell organelles of the nurse cells are transfered to the oocyte through the ring canal at the early oogenesis. 2) At stage 2, the nuclear envelope of oocyte nucleus is less infolding than that of nurse cell nucleus. In the oocyte cytoplasm a large number of ribosomes are observed. 3) At stage 3 and 4, many micropinocytotic vesicles are observed in the oocyte cytoplasm. These vesicles are fused together to form large proteid yolks. 4) At stage 5, the vitelline membrane is laid down in the intercellular space between the follicle cells and oocyte. 5) At stage 6, the chorion is formed by the follicle cells. 6) A micropyle and a number of aeropyle are observed on the surface of a mature egg.
금속 폼 미세 구조의 수치적 연구를 통한 다공성 모델 고찰
김창환(C.H. Kim),김구환(K.H. Kim),우민호(M.N. Woo),이정민(J.M. Lee) 한국전산유체공학회 2012 한국전산유체공학회 학술대회논문집 Vol.2012 No.5
Porous media have been frequently used as flow uniformity devices and many types of reactors in chemical processes. In recent year, porous applications are more widely expanded with increasing the issue of environmental protection and reducing energy. Porous media is not only applied to automotive after treatment system but also used to reduction equipment in plants. There are several numerical approaches for this kind of media. First, 0-D or 1-D model has been developed to calculate the chemical reaction, heat and mass transfer. This focuses on total conversion and systematical control of the process. On the other hand, CFD analysis can deal with the effect of geometry and consider more various conditions. And most CFD currently simulate these devices using porous model. Porous model evaluates the flow field through the material using the ratio between volume occupied by fluid and solid In the porous model, flow through the material is only focused on pressure drop in general. However, flow patterns in the structure are quite different from the types of material. And this affects the characteristics of heat and mass transfer. Considering this kind of effects, porous model analysis can achieve more reasonable results. In this paper, metal foam is considered as porous media, and porous media will be modeled by using pore scale approach rather than experimental calibration. Through the simulation of the flow and heat transfer in pore scale, the availability of porous model will be discussed. Moreover we will also discuss total heat transfer coefficient of porous media with analyzing conjugate heat transfer characteristics in the porous structure. Through the research, we can substitute for the process to find out model constant, which is currently depends on the experiment.
누에나방(Bombyx mori L.) 종령유충일용 전환기(轉換期)의 전흉선(前胸腺) 분필세포(分泌細胞)의 미세구조적 변화
오수자,김지현,김창환,김우갑,Oh, S.J.,Kim, J.H.,Kim, C.W.,Kim, W.K. 한국현미경학회 1982 Applied microscopy Vol.12 No.2
In order to define the morphological changes of the secreting cells of prothoracic gland during larva-pupal molt, ultrastructural observations were carried out using Bombyx mori L. as the experimental material. At first stage of present experiment, 4 day old 5th instar larva, the polyhedral secreting cells were centrally located in the prothoracic gland surrounded by the connective sheath. The secreting cells were attached to the neighboring cells by the prominent desmosomes, and the plasma membrane contacted with connective sheath were highly infolded. In cytoplasm, the most of the cell organelles, such as rod-like mitochondria, rough surfaced endoplasmic reticulum, ribosome were developed. As the stages advance from larva to pupa, general feature of the secreting cells were retained, but structural changes of the various cytoplasmic organelles-ribosome, rough surfaced endoplasmic reticulum, mitochondria, Golgi apparatus, lamellar body, and vesicle-were noted. In the perinuclear cytoplasm of the secreting cells at the stage of 6 day old 5th instar larva, it is peculiar that only a large amount of ribosomes were distributed and the other organelles were retreated from the juxtanuclear region. Just before and after spining cocoon, these features were more remarkable. Rough surfaced endoplasmic reticulum were gradually increased from the stage just before spining cocoon to the pharate pupa. Rod-like mitochondria with irregular cristae and the matrix showing low density were distributed throughout the cytoplasm in the secreting cells of the 4 day old 5th instar larva. Sometimes, longitudinally distended and curved mitochondria were observed. At the stage of pharate pupa, most of mitochondria were deformed. The rod-like mitochondria of the secreting cells of pupal prothoracic gland were narrower than those of 4 day old 5th instar larva, and the electron density of the mitochondrial matrix is increased in pupa. Golgi apparatus were a few in number in both stages, last instar larva and spining cocoon. In stages of the pharate pupa, the Golgi apparatus were frequently observed. Cytoplasmic vesicles were observed for the first time in the secreting cells of one day after spining cocoon, and the number and the size of cytoplasmic vesicles were distinctly increased inpharate pupa and just after pupation. In the secretory cells of the PG, it in concluded that the RER was closely related to syntheting the enzymes seem to produce the ecdysone.
배추흰나비 (Pieris rapae L.)와 누에나방(Bombyx mori L.) 의 변태(變態)에 따른 지방체(脂肪體)의 미세구조(微細構造) 변화(變化)에 관한 연구(硏究)
한성식,김지현,김창환,김우갑,Han, S.S.,Kim, J.H.,Kim, C.W.,Kim, W.K. 한국현미경학회 1982 Applied microscopy Vol.12 No.2
The fat bodies of cabbage worm (Pieris rapae) and silk worm (Bomyx mori) during metamorphosis was comparatively studied by electron microscope. 1. Cell oranelles: Golgi apparatus were not observed in both species. It is observed that RER of cabbage worms initiate to degenerate in prepupa stage with complete degeneration at adult stage, while that of silk worms shows similar degenerative pattern. However, mitochondria of cabbage worms are transformed into autophagic vacuole from prepupa stage until adult stage whereas those of silk worm shows a decrease in number in prepupa stage but maintains a certain level until adult stage. 2. Storage substance in cell: Lipid droplets in cabbage worms were observed to increase in numbers during larval stage but afterward decrease in number with an enlargement in size. However immediately after their pupal stage, they almost disappear. On the contrary lipid droplets in silk worms show rather increase in number until adult stage. Protein storage granules in bothspecies were arised from autophagic vacuoles(lysosome) . Fat cells of cabbage worm in adult stage turn out to be residual bodies which last until final stage, but those of silk worm rapidly decrease. Glycogen particles in both species reach maximum at last larval instar and thee gradually decrease thereafter. 3. Fat body sheath: The average width of fat body sheath was measured to be $0.2{\mu}m$ and $0.6{\mu}m$ and surface of fat cells adjacent to fat body sheath in silk worm is heavily infolded.