토끼 적출 신동맥에 있어서 전기자극에 의한 신경성 수축작용의 neuromodulation 효과

김주헌,홍용근,Kim, Joo-heon,Hong, Yong-geun 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4

To elucidate the neuromodulation of neuropeptide Y and $\alpha,\;\beta$-methylene ATP on the neurogenic contraction of electrical perivascular nerve stimulation and the contractile response of noradrenaline from polygraph in the isolated renal artery of rabbit. 1. The neurogenic contraction induced by perivascular nerve stimulation was the voltage-dependent manner(10-100V) in the isolated renal artery of rabbit. 2. Neuropeptide Y(0.1uM) and $\alpha,\;\beta$-methylene ATP(1uM) increased the contractile responses of noradrenaline in the isolated renal artery of rabbit. 3. Neuropeptide Y(0.1uM) and $\alpha,\;\beta$-methylene ATP(1uM) increased the neurogenic contraction of electrical perivascular nerve stimulation in the isolated renal artery of rabbit. These results suggest that neuropeptide Y and $\alpha,\;\beta$-methylene ATP neuromodulated on the neurogenic contraction of electrical perivascular nerve stimulation on the isolated renal artery of rabbit.

뇨세포검사로 진단된 방광의 배아성 횡문근육종 - 1 증례 보고 -

김주헌,이호,강명재,이동근,김상호,Kim, Joo-Heon,Lee, Ho,Kang, Myoung-Jae,Lee, Dong-Geun,Kim, Sang-Ho 대한세포병리학회 1994 대한세포병리학회지 Vol.5 No.1

Rhabdomyosarcomas are found mainly in young patients, but rare in adults. A correlated cytological and histologic study of one case of embryonal rhabdomyosarcoma is presented. The cytologic appearance of the urine smear corresponded well with the histologic findings. Cytologically, two main cell types were distinguished ; a predominant primitive, small round cell with scant cytoplasm and a large cell with abundant cytoplasm. The cytologic feature proving rhabdomyoblastic differentiation, such as cross-striation, was absent.

개 적출 신동맥에 있어서 histamine receptor의 생리적 특성

김주헌,남윤정,Kim, Joo-heon,Nam, Yun-jeong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.2

To elucidate the physiological characteristics of histamine receptor of the isolated renal artery in dog, effects of various receptor blockers and $Ca^{{+}{+}}$ channel blockers on the contractile responses of histamine were investigated in the isolated renal artery of dog. The results were summarized as follows: 1. Histamine caused the contraction in the isolated renal artery of dog, and the contractile responses increased between the concentration of $5{\times}10^{-6}M$ and $10^{-2}M$ in dose-dependent manner. 2. The contractile response induced by histamine ($10^{-3}M$) was not blocked by pretreatment with atropine ($10^{-6}M$), phentolamine ($10^{-6}M$), propranolol ($10^{-6}M$) or cimetidine ($10^{-6}M$), but was completely blocked by pretreatment with pyrilamine ($10^{-6}M$). 3. The contractility induced by histamine ($10^{-3}M$) was decreased in the $Ca^{{+}{+}}$ free medium. 4. The contractile response induced by histamine ($10^{-3}M$) was markedly inhibited by pretreatment with papaverine ($5{\times}10^{-5}M$) or verapamil ($5{\times}10^{-5}M$). 5. After the pre-contraction induced by norepinephrine ($10^{-7}M$) or prostaglandin $F2{\alpha}$ ($5{\times}10^{-6}M$), the contractile response induced by the additional treatment of histamine ($10^{-3}M$) was markedly increased compared with that of histamine ($10^{-3}M$) alone.

가토의 근위와 원위대장 평활근의 운동성 비교

김주헌,장기철,윤효인,Kim, Joo-heon,Chang, Ki-churl,Yun, Hyo-in 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

To validate the comparision of proximal and distal large intestinal motility, the amplitude and frequency of spontaneous motility, the effect of acetylcholine, the effect of atropine on the response of acetylcholine, the effect of histamine and the effect of pyrilamine and cimetidine on the response of histamine were investigated in rabbit. The results were summarized as follows: 1. The amplitude of spontaneous motility was more powerful on the proximal large intestine than that of the distal large intestine, but the frequency of spontaneous motility was similar on the both proximal and distal large intestine in rabbit. 2. Acetylcholine caused the contraction of proximal and distal large intestine, and the contractile response were increased between the concentration of acetylcholne $10^{-9}$ and $5{\times}10^{-6}M$ and $10^{-7}$ and $10^{-4}M$ on the proximal and distal large intestine, respectively, with dose-dependent manner in rabbit. 3. The contractile response induced by acetylcholine was completely blocked by the post-treatment with cholinergic receptor blocker, atropine $10^{-6}M$. 4. Histamine caused the contraction of proximal and distal large intestine and the contractile response were increased between the concentration of histamine $10^{-9}$ and $5{\times}10^{-5}M$ and $10^{-5}$ and $10^{-3}M$ on the proximal and distal large intestine, respectively, with dose-depend ent manner in rabbit. 5. The contractile response induced by histamine was completely blocked by the pretreatment with $H_1$-receptor blocker, pyrilamine $10^{-6}M$, but not blocked by the pretreatment with $H_2$-receptor blocker, cimetidine $10^{-6}M$.

미성숙 돼지 자궁 평활근의 운동성에 대한 Adenosine Triphosphate의 작용에 있어서 수종의 Receptor 차단제의 영향

김주헌,권종국,김용근,Kim, Joo-heon,Kwun, Jong-kuk,Kim, Yong-keun 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

This study was carried out to investigate the action of ATP, which has been known as the neurotransmitter of noncholinergic- and nonadrenergic-nerve, on the motility of immature pig uterine smooth muscle. The results were summarized as follows; 1. The contraction and the contractile responses caused by ATP were increased in a dose-dependent manner between the concentration of ATP $10^{-6}M$ and $10^{-3}M$. The maximal contractile effect was appeared at the concentration of ATP $10^{-3}M$ and it was 70.2% of 100mM K contraction. 2. The contractile responses induced by ATP ($10^{-4}M$) were not blocked by the pretreatment with cholinergic receptor blocker, atropine ($10^{-6}M$). 3. The contractile responses induced by ATP ($10^{-4}M$) were not blocked by pretreatment with ${\alpha}$-adrenergic receptor blocker, phentolamine ($10^{-6}M$) and ${\beta}$-adrenergic receptor blocker, propranolol ($10^{-6}M$). 4. The contractile response induced by ATP ($10^{-4}M$) was not blocked by the pretreatment with $H_1-receptor$ blocker, pyrilamine ($10^{-6}M$) and $H_2-receptor$ blocker, cimetidine ($10^{-6}M$).

개 회장 평활근에 있어서 Histamine Receptor의 생리적 특성에 관한 연구

김주헌,박영배,권종국,Kim, Joo-heon,Park, Yong-bae,Kwun, Jong-kuk 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

To validate the physiological properties of the histamine receptors of ileal smooth muscle in dog, the effects of adrenergic-, cholineric-, and H-receptor antagonists on the responses of ileal smooth muscle strips to histamine were investigated. The results were summarized as follows; 1. Histamine caused the contraction of ileal smooth muscle and the contractile responses were increased between the concentration of histamine $10^{-7}M$ and $10^{-5}M$ with dose-dependent manner in dog. 2. The shorter the treatment interval of histamine, the lower the contractile activity until the treatment interval extended to 40 minutes. 3. The contractile response induced by histamine was completely blocked by the pre treatment with a $H_1$-receptor blocker, chlorpheniramine and not by the pretreatment with a $H_2$-receptor blockers cimetidine. 4. The contractile response induced by histamine was not blocked by the pretreatment with a cholinergic receptor blocker, atropine. 5. The contractile response induced by histamine was not blocked by the pretreatment with an ${\alpha}$-adrenergic receptor blocker, phenoxybenzamine, or a ${\beta}$-adrenergic receptor blocker, propranolol. From these results, it was suggested that the contraction induced by histamine was elicited through $H_1$-receptor on the ileal smooth muscle in dog.

Ethanol 이 고양이 신장 Na-K-ATPase 활성에 미치는 영향

김주헌,김용근,Kim, Joo-Heon,Kim, Yong-Keun 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.1

The effects of ethanol on Na-K-ATPase activity were investigated with cat kidney homogenate. The results were summarized as follows: 1. Na-K-ATPase activity was inhibited with dose-dependent manner by ethanol of higher concentration than 1%, and showed an estimated $I_{50}$ (the inhibitor concentration to cause 50% inhibition) of 7.5%. 2. Hydrolysis of ATP was linear with the incubation time in the absence and presence of 8% ethanol, whereas it was different with preincubation time in the presence of 15% ethanol. 3. Inhibition of Na-K-ATPase activity by ethanol was not affected by increased enzyme concentration, and showed the reversibility of the inhibitory pattern. 4. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed that ethanol had both properties of classical competitive inhibition for $Mg^{{+}{+}}$ or $K^+ and non-competitive inhibition for ATP or $Na^+$. 5. Arrhenius plot yield two break point at $21^{\circ}$ and $30^{\circ}C$ in the absence of ethanol, whereas showing only one break point at $18^{\circ}C$ in the presence of 8% ethanol. These results suggested that ethanol inhibited Na-K-ATPase activity reversible through a disturbance of microenvironment of lipids associated with the enzyme.

토끼 적출 위 평활근의 운동성에 대한 transmural stimulation의 효과

김주헌,심철수,홍용근,Kim, Joo-heon,Shim, Cheol-soo,Hong, Yong-geun 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

To elucidate the action of the cholinergic and adrenergic nerve on isolated gastric fundus smooth muscle of rabbit, the effects of electrical transmural stimulation were investigated in the presence of atropine, cholinergic receptor blocker; phentolamine, nonselective ${\alpha}$-adrenergic receptor blocker; propranolol, nonselective ${\beta}$-adrenergic receptor blocker and L-arginine from the isometric contraction of physiological recording system. 1. The contractile response induced by electrical transmural stimulation was increased as the frequency(1~32Hz)-dependent manner on the isolated gastric fundus smooth muscle. 2. The contractile response induced by electrical transmural stimulation was markedly inhibited by the pretreatment of atropine($1{\mu}M$). 3. The contractile response induecd by electrical transmutal stimulation was inhibited by the pretreatment of phentolamine($1{\mu}M$). 4. The relaxative response induced by electrical transmural stimulation on presence of atropine ($1{\mu}M$) was inhibited by the pretreatment of propranolol($1{\mu}M$). 5. The relaxative responses on precontraction induced by histamine($10{\mu}M$) with guanethidine ($50{\mu}M$) and atropine($1{\mu}M$) by electrical transmural stimulation were increased by L-arginine (1mM). These findings suggest that it was the excitatory action of cholinergic and ${\alpha}$-adrenergic nerve, and the inhibitory action of ${\beta}$-adrenergic nerve and nonadrenergic noncholinergic nerve on the isolated gastric fundus smooth muscle of rabbit.

돼지 심관상동맥의 이완작용에 대한 purinergic 신경의 효과

김주헌,심철수,전석철,Kim, Joo-heon,Shim, Cheol-soo,Jeon, Seok-cheol 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3

To elucidate the effects of purinergic nerve on relaxation of pig coronary artery, the effects of ATP, 2-methylthio ATP and electrical perivascular nerve stimulation were investigated from physiograph on the isolated coronary artery of pig. The results btained were as follows; 1. The relaxative responses induced by perivascular nerve stimulation(20V, 0.5msec, 10sec) were the frequency(1~8Hz) dependent manner with phentolamine($10^{-5}M$) and atropine($10^{-6}M$) on isolated coronary artery of pig. 2. The relaxative responses induced. by adenosine($10^{-7}{\sim}5{\times}10^{-3}M$) or ATP($10^{-7}{\sim}5{\times}10^{-5}M$) on precontraction with histamine($10^{-5}M$) were the dose-dependent manner, but the contractile responses were often induced by ATP($10^{-4}M$ and $10^{-3}M$). 3. The relaxative responses induced by 2-methylthio ATP($2.5{\times}10^{-8}{\sim}2.5{\times}10^{-6}M$) on precontraction with histamine($10^{-5}M$) were the dose-dependent manner. 4. The relaxative response induced by 2-methylthio ATP($10^{-7}M$) on precontraction with histamine($10^{-5}M$) was completely blocked by the pretreatment with $P_{2Y}$-purinoceptor blocker, reactive blue 2($10^{-4}M$). 5. The neurogenic relaxative response induced by perivascular nerve stimulation(20V, 8Hz, 0.5msec, 10sec) was weakly inhibited by the pretreatment with ${\beta}$-adrenoceptor blocker, propranolol($10^{-5}M$) and blocked by the addition with $P_{2Y}$-purinoceptor blocker, reactive blue 2($10^{-4}M$). The results suggest that the purinergic nerve is innervated, and its relaxative response was mediated by $P_{2Y}$-purinoceptor on isolated coronary artery in pig.

Purinergic innervation on the isolated renal artery of rabbit

김주헌,김용근,Kim, Joo-heon,Kim, Yong-keun The Korean Society of Veterinary Science 1991 大韓獸醫學會誌 Vol.31 No.4

가토 적출 신동맥에 있어서 purinergic 신경 분포를 perivascular nerve stimulation을 통해서 확인하였다. 1. 가토 적출 신동맥은 perivascular nerve stimulation에 의해 수축성 반응을 나타내었다. 2. Perivascular nerve stimulation에 의한 수축현상은 자극의 크기와 자극의 빈도에 의존적이었으며, 내피세포의 유무에는 영향을 받지 않았다. 3. Perivascular nerve stimulation에 의한 수축현상은 prazosin$(1{\mu}M)$에 의해 강하게 억제되어졌으며, tetrodotoxin$(1{\mu}M)$ 또는 ${\alpha},{\beta}-methylene$ATP$(10{\mu}M)$에 의한 $P_{2x}-purinoceptor $ desensitization에 의해 완전히 수축현상이 사라졌다. 이와같은 결과는 가토 신동맥에는 purinergic신경이 분포되어 있으며, perivascular nerve stimulation에 의해 purinergic 신경전달물질이 norepinephrine과 함께 유리되어지는 것을 시사하고 있다.